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The map label for this gene is miaB [H]

Identifier: 115352797

GI number: 115352797

Start: 3023892

End: 3025265

Strand: Direct

Name: miaB [H]

Synonym: Bamb_2746

Alternate gene names: 115352797

Gene position: 3023892-3025265 (Clockwise)

Preceding gene: 115352796

Following gene: 115352798

Centisome position: 85.02

GC content: 65.21

Gene sequence:

>1374_bases
ATGACGAAAAAAGTTTACGTAAAGACCTTCGGCTGCCAGATGAACGAGTACGACTCGGACAAGATGGTGGACGTGCTCAA
TGCGGCCGAAGGCCTCGAAAAGACCGATACCCCGGAAGACGCGGACATCATCCTGTTCAACACGTGCTCGGTGCGTGAGA
AGGCGCAGGAAAAGGTGTTCTCCGACCTCGGCCGCGTGCGCGAGCTGAAGGAAGCGAAGCCGGGCCTGCTGATCGGCGTC
GGCGGCTGCGTCGCCAGCCAGGAAGGCGCGTCGATCGTGTCGCGCGCACCGTACGTCGACCTCGTGTTCGGCCCGCAGAC
GCTGCACCGCCTGCCGCAGATGATCGACCAGCGCCGCGCGAGCGGCCGTGCACAGGTCGACATCTCGTTCCCCGAGATCG
AGAAGTTCGACCACCTGCCGCCCGCGCGCGTCGAGGGGCCGAGCGCGTTCGTGTCGATCATGGAAGGCTGTTCGAAGTAC
TGCAGCTACTGCGTCGTGCCGTACACGCGCGGCGACGAAGTGTCGCGCCCGCTCGACGACGTGCTGACCGAAGTGGCCGG
CCTCGCCGACCAGGGCGTGCGCGAAGTCACGCTGCTCGGCCAGAACGTGAATGCATACCGCGGCGCGCTGACGGCCGGCT
CGACGGAAATCGCCGATTTCGCGACGCTGATCGAATACGTCGCCGACATCCCCGGCATCGAGCGCATCCGCTACACGACG
TCGCACCCGAAGGAATTCACGCAGCGCCTGATCGACACCTACGCGAAGGTGCCGAAGCTCGTGAGCCACCTGCACCTGCC
GGTCCAGCACGGCTCCGACCGCATCCTGATGGCGATGAAGCGCGGCTACACGGTGCTCGAATACAAGTCGGTGATCCGCA
AGCTGCGCGCGATCCGCCCGGACCTGTCGCTGTCGACCGACATGATCGTCGGCTTCCCCGGCGAGACCGAGGAGGATTTC
GACAAGATGATGGCGCTCGTGCACGAGATGAGCTACGACACCAGCTTCTCGTTCATCTACAGCCCGCGCCCCGGCACGCC
GGCCGCGAACCTGCACGACGACACGCCGCGCGAGGTCAAGCTCAAACGCCTGCAACATCTGCAGGCGACCATCGAGGAAA
ACGTCGCGCGCATCAGCCGGTCGATGGTCGGGAAGGTCGAGCGGATCCTCGTCGAGGGCCCGTCGCGCAAGGATCCGAAC
GAACTGTCGGGCCGCACCGAGAACAACCGGGTCGTGAATTTCCCGGCGCCGCTCGCGTCGCACCCGCGCCTGATCGGCCA
GATGATCGACGTGAAGATCAACCATGCGTACCCGCATTCGCTGCGCGGCGAGCTGCTGCTCGTGAGCGACGACGCGAGCG
CGGCCACCCATTGA

Upstream 100 bases:

>100_bases
TCCGCCACGCGGCGCCGGCACGAGCCGCTGCATCGCTGCAGCCGTCCCGATACACCCTTGACGACGCCCCCAGGTCAACC
ACTGCGAACGGCGAATCCCC

Downstream 100 bases:

>100_bases
CCGACTGACGCCCGACAGGAGCCCGACGCTACTTTGAAGACCGTCCAAGCACTGGAATTCACCGCGCCGCGCGAAGACAA
CGCGCGCCTCGCCAACCTCT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDLGRVRELKEAKPGLLIGV
GGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDQRRASGRAQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKY
CSYCVVPYTRGDEVSRPLDDVLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSTEIADFATLIEYVADIPGIERIRYTT
SHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPDLSLSTDMIVGFPGETEEDF
DKMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPREVKLKRLQHLQATIEENVARISRSMVGKVERILVEGPSRKDPN
ELSGRTENNRVVNFPAPLASHPRLIGQMIDVKINHAYPHSLRGELLLVSDDASAATH

Sequences:

>Translated_457_residues
MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDLGRVRELKEAKPGLLIGV
GGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDQRRASGRAQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKY
CSYCVVPYTRGDEVSRPLDDVLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSTEIADFATLIEYVADIPGIERIRYTT
SHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPDLSLSTDMIVGFPGETEEDF
DKMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPREVKLKRLQHLQATIEENVARISRSMVGKVERILVEGPSRKDPN
ELSGRTENNRVVNFPAPLASHPRLIGQMIDVKINHAYPHSLRGELLLVSDDASAATH
>Mature_456_residues
TKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDLGRVRELKEAKPGLLIGVG
GCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDQRRASGRAQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYC
SYCVVPYTRGDEVSRPLDDVLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSTEIADFATLIEYVADIPGIERIRYTTS
HPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPDLSLSTDMIVGFPGETEEDFD
KMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPREVKLKRLQHLQATIEENVARISRSMVGKVERILVEGPSRKDPNE
LSGRTENNRVVNFPAPLASHPRLIGQMIDVKINHAYPHSLRGELLLVSDDASAATH

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=37.0292887029289, Blast_Score=299, Evalue=4e-81,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=37.0292887029289, Blast_Score=298, Evalue=9e-81,
Organism=Homo sapiens, GI93277076, Length=459, Percent_Identity=27.4509803921569, Blast_Score=173, Evalue=2e-43,
Organism=Escherichia coli, GI1786882, Length=451, Percent_Identity=62.0842572062084, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI1787057, Length=454, Percent_Identity=29.9559471365639, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17553146, Length=464, Percent_Identity=35.3448275862069, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI71996771, Length=300, Percent_Identity=30, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21356207, Length=482, Percent_Identity=32.9875518672199, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI19922432, Length=467, Percent_Identity=28.6937901498929, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50785; Mature: 50653

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVF
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
SDLGRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDQRRA
HHHHHHHHHHHCCCCEEEEECCHHCCCCCHHHHHCCCEEEEEECHHHHHHHHHHHHHHHH
SGRAQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD
CCCEEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHH
VLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSTEIADFATLIEYVADIPGIERIRYTT
HHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECC
SHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEHHHCCCCHHHHHHHHHHHHHHCC
DLSLSTDMIVGFPGETEEDFDKMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPREVK
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHH
LKRLQHLQATIEENVARISRSMVGKVERILVEGPSRKDPNELSGRTENNRVVNFPAPLAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEEECCCCCCC
HPRLIGQMIDVKINHAYPHSLRGELLLVSDDASAATH
CHHHHHHHHHEEECCCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
TKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVF
CCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
SDLGRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDQRRA
HHHHHHHHHHHCCCCEEEEECCHHCCCCCHHHHHCCCEEEEEECHHHHHHHHHHHHHHHH
SGRAQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD
CCCEEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHH
VLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSTEIADFATLIEYVADIPGIERIRYTT
HHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECC
SHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEHHHCCCCHHHHHHHHHHHHHHCC
DLSLSTDMIVGFPGETEEDFDKMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPREVK
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHH
LKRLQHLQATIEENVARISRSMVGKVERILVEGPSRKDPNELSGRTENNRVVNFPAPLAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEEECCCCCCC
HPRLIGQMIDVKINHAYPHSLRGELLLVSDDASAATH
CHHHHHHHHHEEECCCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA