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The map label for this gene is dmlR [H]

Identifier: 115352796

GI number: 115352796

Start: 3022682

End: 3023614

Strand: Direct

Name: dmlR [H]

Synonym: Bamb_2745

Alternate gene names: 115352796

Gene position: 3022682-3023614 (Clockwise)

Preceding gene: 115352792

Following gene: 115352797

Centisome position: 84.99

GC content: 70.74

Gene sequence:

>933_bases
ATGGATCGACTGGGCGACATCCGGCTGTTCGTCGAGGCGGCGGAACTGGGCAGCCTGTCAGCGGCCGGGCGCAAGCTGAA
CCTCACGCCGGCCGCCGCGAGCGCGCGCCTCGCGAAGCTCGAGGCCAAGGTCGCGACGCGACTGTTCGAGCGCTCCACCA
GACAGCTGCGGCTCACCGACGAAGGCCGGCTCTACCTGAACTGCTGCCGGCAGGCGCTGCAGGCGCTCGACGACGCGCAC
GCGCTGCTGCAGGAAGGCCGCAACGTGGTGGCCGGCAAGGTGCGGCTGTCGTCGACGTCCGATTTCGGCCGCAACCAGCT
GCTCGACTGGCTCGACGAATTCACCACGCTGCATCCGGGCGTCACGTTCTCGCTGACGGCGTCCGATTCGGCGTCGAACC
TGTGGCAGGACGAAATCGACCTCGCGATCCGCTTCGCCGCGCCACCCGACGGCGCGTTGATCGCGCGCCGGCTGGCCGCG
AACCGGCGCGTACTGTGCGCGGCGCCGTCGTTCGTCGCGCGCCACGGCGTGCCGGCCGATCCGCACGATCTGGCCCGCTT
TCCCTGCAACGTGATCACGATCGCGTCGGGCCCGATGAACACGTGGCGCTTCACGCGCGGCGACGAAGTCCAGACGCACA
CGGTGCCGGTGTCGACCGCGTTCGAGACCAACGACGGCGGCCTCACCCGCGAATGGACGCTGCGCGGCCACGGCATCGCG
CTGAAGTCGCTGTGGGACATCGCCGACGACGTGCGCGCCGGCCGGCTGCGCGTGCTGCTGCCCGACTGGCGGCACCAGGA
CGCGCCGCTGCACGCGATCTATCACAGCAAGCGCTACATGGCGCCGCGCGTGCGCGTGCTGCTCGACTTCCTCGCCGAAC
GCTTCGCGCGCGAGGAAGCGGCGCTCGACGACCTGCTGAACGCGTGCCGCTGA

Upstream 100 bases:

>100_bases
CGCACAAAAAAGCAGGATGTGCGCGCATTGTCCCGAATACGCCTATCTTTGATAATTGGCGTGGCATTTGAACCATTTTC
AAAAAATTCTGGAAAATCCC

Downstream 100 bases:

>100_bases
CGCCGACACTGACGCTCGCGTGCGCCGCCCTATCAGGGTCGGAGCGGGCAGAACGGCGTCGCGCGGCCGCAACCCGCCGC
CCGCCCGGGACGGCGCGTAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MDRLGDIRLFVEAAELGSLSAAGRKLNLTPAAASARLAKLEAKVATRLFERSTRQLRLTDEGRLYLNCCRQALQALDDAH
ALLQEGRNVVAGKVRLSSTSDFGRNQLLDWLDEFTTLHPGVTFSLTASDSASNLWQDEIDLAIRFAAPPDGALIARRLAA
NRRVLCAAPSFVARHGVPADPHDLARFPCNVITIASGPMNTWRFTRGDEVQTHTVPVSTAFETNDGGLTREWTLRGHGIA
LKSLWDIADDVRAGRLRVLLPDWRHQDAPLHAIYHSKRYMAPRVRVLLDFLAERFAREEAALDDLLNACR

Sequences:

>Translated_310_residues
MDRLGDIRLFVEAAELGSLSAAGRKLNLTPAAASARLAKLEAKVATRLFERSTRQLRLTDEGRLYLNCCRQALQALDDAH
ALLQEGRNVVAGKVRLSSTSDFGRNQLLDWLDEFTTLHPGVTFSLTASDSASNLWQDEIDLAIRFAAPPDGALIARRLAA
NRRVLCAAPSFVARHGVPADPHDLARFPCNVITIASGPMNTWRFTRGDEVQTHTVPVSTAFETNDGGLTREWTLRGHGIA
LKSLWDIADDVRAGRLRVLLPDWRHQDAPLHAIYHSKRYMAPRVRVLLDFLAERFAREEAALDDLLNACR
>Mature_310_residues
MDRLGDIRLFVEAAELGSLSAAGRKLNLTPAAASARLAKLEAKVATRLFERSTRQLRLTDEGRLYLNCCRQALQALDDAH
ALLQEGRNVVAGKVRLSSTSDFGRNQLLDWLDEFTTLHPGVTFSLTASDSASNLWQDEIDLAIRFAAPPDGALIARRLAA
NRRVLCAAPSFVARHGVPADPHDLARFPCNVITIASGPMNTWRFTRGDEVQTHTVPVSTAFETNDGGLTREWTLRGHGIA
LKSLWDIADDVRAGRLRVLLPDWRHQDAPLHAIYHSKRYMAPRVRVLLDFLAERFAREEAALDDLLNACR

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=293, Percent_Identity=32.7645051194539, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI1789639, Length=296, Percent_Identity=30.4054054054054, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=32.5259515570934, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1789440, Length=290, Percent_Identity=31.3793103448276, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=29.9319727891156, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1787128, Length=292, Percent_Identity=27.7397260273973, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1786448, Length=253, Percent_Identity=25.296442687747, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788706, Length=175, Percent_Identity=27.4285714285714, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789173, Length=254, Percent_Identity=24.4094488188976, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI157672245, Length=143, Percent_Identity=28.6713286713287, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=172, Percent_Identity=25.5813953488372, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34422; Mature: 34422

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLGDIRLFVEAAELGSLSAAGRKLNLTPAAASARLAKLEAKVATRLFERSTRQLRLTD
CCCCCCEEEEEEHHHHCCHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
EGRLYLNCCRQALQALDDAHALLQEGRNVVAGKVRLSSTSDFGRNQLLDWLDEFTTLHPG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC
VTFSLTASDSASNLWQDEIDLAIRFAAPPDGALIARRLAANRRVLCAAPSFVARHGVPAD
CEEEEECCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCEEEEECCHHHHHCCCCCC
PHDLARFPCNVITIASGPMNTWRFTRGDEVQTHTVPVSTAFETNDGGLTREWTLRGHGIA
HHHHHHCCCCEEEEECCCCCCCEECCCCCCEEEEEEEEEEEECCCCCCEEEEEECCCCEE
LKSLWDIADDVRAGRLRVLLPDWRHQDAPLHAIYHSKRYMAPRVRVLLDFLAERFAREEA
HHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHH
ALDDLLNACR
HHHHHHHHCC
>Mature Secondary Structure
MDRLGDIRLFVEAAELGSLSAAGRKLNLTPAAASARLAKLEAKVATRLFERSTRQLRLTD
CCCCCCEEEEEEHHHHCCHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
EGRLYLNCCRQALQALDDAHALLQEGRNVVAGKVRLSSTSDFGRNQLLDWLDEFTTLHPG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC
VTFSLTASDSASNLWQDEIDLAIRFAAPPDGALIARRLAANRRVLCAAPSFVARHGVPAD
CEEEEECCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCEEEEECCHHHHHCCCCCC
PHDLARFPCNVITIASGPMNTWRFTRGDEVQTHTVPVSTAFETNDGGLTREWTLRGHGIA
HHHHHHCCCCEEEEECCCCCCCEECCCCCCEEEEEEEEEEEECCCCCCEEEEEECCCCEE
LKSLWDIADDVRAGRLRVLLPDWRHQDAPLHAIYHSKRYMAPRVRVLLDFLAERFAREEA
HHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHH
ALDDLLNACR
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]