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The map label for this gene is glpF [H]

Identifier: 115352783

GI number: 115352783

Start: 3010274

End: 3010990

Strand: Direct

Name: glpF [H]

Synonym: Bamb_2732

Alternate gene names: 115352783

Gene position: 3010274-3010990 (Clockwise)

Preceding gene: 115352782

Following gene: 115352787

Centisome position: 84.64

GC content: 67.78

Gene sequence:

>717_bases
ATGTCACCTTATATTGCGGAATTCATTGGCACGGCGATCCTCGTGCTGCTCGGCAACGGCGCGGTCGCAAACGTGCTGCT
TGCGAAGACCAAGGGCAAAGGCGCGGATCTGATCGTCATCGTGATGGGCTGGGCGATGGCGGTGTTCGTCGCTGTCTACG
TGACGGCGTCGTTCAGCGGCGCGCACCTGAACCCGATCGTCACGATCAGCCTCGCGCTCGCGGGCAAGTTCGCGTGGTCG
AAGGTCGGCGGCTACATTGTTGCGCAGATGCTCGGCGGGATGGCCGGCGCGTTGCTCGTGTGGCTCGCGTATCGCCAGCA
CTTCGCGAAGGAAGCCGATGCGGACCTGAAGCTCGCGGTGTTCTGCACGGCGCCCGCGATCCGCAGCGTCACGCACAACG
TGCTGACCGAAGCGATCTGCACGTTCGTGCTGATTCTCGGCGTGCTGTACCTCGCGTCGCCGCAGGTCGGCCTCGGCGCG
CTCGACGCGCTGCCCGTCGGCTTGCTCGTGCTCGGCATCGGCATCTCGCTCGGCGGCCCGACCGGCTATGCGATGAGCCC
GGCGCGCGACCTGTCGCCGCGCCTGATGCATGCGCTGCTGCCGATCCCCGGCAAGCGCGACAGCGACTGGCGCTATGCGT
GGGTGCCGGTGATCGGCCCGCTGCTCGGCGGCGCCGCCGCGGCCGGCCTGTATCTGCGTCTGCACGCGATGGCCTGA

Upstream 100 bases:

>100_bases
CGACGACACGCAGTAACCGCGGTACATCTAAACAAACGAAATACAACAACACCGGCGGACCGCGCGGCGCGCGAGTCCGC
CGTCATCTACGAGAGACAAC

Downstream 100 bases:

>100_bases
GCAACGTTTCTTCACTCGAGTGCGAGCGCGCGGCAGCGGCTGCCGCGCGCTTACGGTTCTTCCGCCCTTCTTCCTCCTCC
CTCTTCCCTTCGGGCACGGC

Product: MIP family channel protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSPYIAEFIGTAILVLLGNGAVANVLLAKTKGKGADLIVIVMGWAMAVFVAVYVTASFSGAHLNPIVTISLALAGKFAWS
KVGGYIVAQMLGGMAGALLVWLAYRQHFAKEADADLKLAVFCTAPAIRSVTHNVLTEAICTFVLILGVLYLASPQVGLGA
LDALPVGLLVLGIGISLGGPTGYAMSPARDLSPRLMHALLPIPGKRDSDWRYAWVPVIGPLLGGAAAAGLYLRLHAMA

Sequences:

>Translated_238_residues
MSPYIAEFIGTAILVLLGNGAVANVLLAKTKGKGADLIVIVMGWAMAVFVAVYVTASFSGAHLNPIVTISLALAGKFAWS
KVGGYIVAQMLGGMAGALLVWLAYRQHFAKEADADLKLAVFCTAPAIRSVTHNVLTEAICTFVLILGVLYLASPQVGLGA
LDALPVGLLVLGIGISLGGPTGYAMSPARDLSPRLMHALLPIPGKRDSDWRYAWVPVIGPLLGGAAAAGLYLRLHAMA
>Mature_237_residues
SPYIAEFIGTAILVLLGNGAVANVLLAKTKGKGADLIVIVMGWAMAVFVAVYVTASFSGAHLNPIVTISLALAGKFAWSK
VGGYIVAQMLGGMAGALLVWLAYRQHFAKEADADLKLAVFCTAPAIRSVTHNVLTEAICTFVLILGVLYLASPQVGLGAL
DALPVGLLVLGIGISLGGPTGYAMSPARDLSPRLMHALLPIPGKRDSDWRYAWVPVIGPLLGGAAAAGLYLRLHAMA

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=242, Percent_Identity=34.297520661157, Blast_Score=120, Evalue=7e-28,
Organism=Homo sapiens, GI4826645, Length=238, Percent_Identity=34.453781512605, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI22538420, Length=247, Percent_Identity=31.5789473684211, Blast_Score=104, Evalue=6e-23,
Organism=Homo sapiens, GI4502187, Length=241, Percent_Identity=30.2904564315353, Blast_Score=94, Evalue=9e-20,
Organism=Escherichia coli, GI1790362, Length=248, Percent_Identity=35.0806451612903, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71994009, Length=228, Percent_Identity=32.8947368421053, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17544068, Length=241, Percent_Identity=31.5352697095436, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17558372, Length=243, Percent_Identity=30.8641975308642, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17531429, Length=240, Percent_Identity=27.0833333333333, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17531431, Length=240, Percent_Identity=27.0833333333333, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71992966, Length=251, Percent_Identity=28.2868525896414, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71993722, Length=230, Percent_Identity=29.5652173913043, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=211, Percent_Identity=32.2274881516588, Blast_Score=93, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322985, Length=271, Percent_Identity=27.6752767527675, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24762342, Length=236, Percent_Identity=27.1186440677966, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19922828, Length=231, Percent_Identity=29.8701298701299, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24705; Mature: 24574

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPYIAEFIGTAILVLLGNGAVANVLLAKTKGKGADLIVIVMGWAMAVFVAVYVTASFSG
CCCHHHHHHHHHHHHHHCCCHHHHHEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCC
AHLNPIVTISLALAGKFAWSKVGGYIVAQMLGGMAGALLVWLAYRQHFAKEADADLKLAV
CCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
FCTAPAIRSVTHNVLTEAICTFVLILGVLYLASPQVGLGALDALPVGLLVLGIGISLGGP
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
TGYAMSPARDLSPRLMHALLPIPGKRDSDWRYAWVPVIGPLLGGAAAAGLYLRLHAMA
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
SPYIAEFIGTAILVLLGNGAVANVLLAKTKGKGADLIVIVMGWAMAVFVAVYVTASFSG
CCHHHHHHHHHHHHHHCCCHHHHHEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCC
AHLNPIVTISLALAGKFAWSKVGGYIVAQMLGGMAGALLVWLAYRQHFAKEADADLKLAV
CCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
FCTAPAIRSVTHNVLTEAICTFVLILGVLYLASPQVGLGALDALPVGLLVLGIGISLGGP
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
TGYAMSPARDLSPRLMHALLPIPGKRDSDWRYAWVPVIGPLLGGAAAAGLYLRLHAMA
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]