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The map label for this gene is dgoD [H]

Identifier: 115352770

GI number: 115352770

Start: 2995302

End: 2996450

Strand: Direct

Name: dgoD [H]

Synonym: Bamb_2719

Alternate gene names: 115352770

Gene position: 2995302-2996450 (Clockwise)

Preceding gene: 115352768

Following gene: 115352772

Centisome position: 84.22

GC content: 66.75

Gene sequence:

>1149_bases
ATGAAAATCATCCGCCTCGAAACCTTCGTCGTCCCGCCGCGCTGGCTGTTCCTCAAGATCGAAACCGACGAGGGCATCGT
CGGCTGGGGCGAGCCGGTGGTCGAGGGCCGCGCGCACACGGTCGAAGCCGCCGTGCAGGAACTCGCCGACTATCTGGTCG
GCAAGGATCCGCTGCTGATCGAGGATCACTGGCAGGTGATGTACCGTGCGGGCTTCTACCGCGGCGGCCCGATCATGATG
AGCGCGATCGCGGGCGTCGACCAGGCGCTGTGGGACATCAAGGGCAAGCATCACGGCGTACCGGTGCACGCGCTGCTCGG
CGGACAGGTGCGCGACCGCATCAAGGTGTATTCGTGGATCGGCGGCGACCGGCCGAGCGATGTCGCGAACAACGCGCGCG
CGGTGGTCGAGCGCGGCTTCAAGGCCGTGAAGATGAACGGCTCCGAGGAGCTGCAGATCGTCGACACCTACGACAAGGTC
GAGCAGGTGATCGCGAACGTCGCGGCGGTGCGCGACGCGGTCGGCCCGCACGTCGGGATCGGCGTCGACTTCCATGGCCG
CGTGCACAAGCCAATGGCGAAGGTGCTCGCGAAGGAGCTCGATCCGTACAAGCTGATGTTCATCGAGGAGCCGGTGCTGT
CGGAGAACGCCGAGGCGCTGCGCGACATCGTGAACCAGACCAACACGCCGATCGCGCTCGGCGAGCGGCTCTACTCGCGC
TGGGACTTCAAGCACATCCTCGCGGGCGGCTACGTCGACATCATCCAGCCCGACGCGTCGCACGCGGGCGGCATCACCGA
GTGCCGCAAGATCGCGACGCTCGCGGAAAGCTACGACGTCGCGCTCGCGCTGCACTGCCCGCTCGGCCCGATCGCGCTGG
CCGCGTGCCTGCAGCTCGATGCGGTCAGCTACAACGCGTTCATCCAGGAACAGAGCCTCGGCATCCACTACAACCAGGGG
AACGACCTGCTCGACTACCTGCGCAACCCCGAGGTGTTCCGCTACGAAGACGGCTTCGTCGCGATCCCGCAAGGGCCGGG
GCTCGGCATCGACGTCAACGAGGAGAAGGTGCGCGAGATGGCGAAGACGGGCCATCGCTGGCGCAATCCGGTGTGGCGCC
ATGCGGACGGCAGCGTCGCCGAGTGGTGA

Upstream 100 bases:

>100_bases
ATTAATACTATTTTTTATCACGCTACGCTAGGATAACCGTCACGTACTCGACGGCGCCGCATCCGCACGTCCGCGCCGCG
CAACCCAGGAGACTCGCGAC

Downstream 100 bases:

>100_bases
TGCGGAGGTGAAGTGAGAGGAGATGCATTGCGCGGCGGACCACCCGCGCATTGCAGGTCGAGACGGCCGCCCGACAGGCG
GCCGCTTTTACATCCGCGGC

Product: galactonate dehydratase

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKIIRLETFVVPPRWLFLKIETDEGIVGWGEPVVEGRAHTVEAAVQELADYLVGKDPLLIEDHWQVMYRAGFYRGGPIMM
SAIAGVDQALWDIKGKHHGVPVHALLGGQVRDRIKVYSWIGGDRPSDVANNARAVVERGFKAVKMNGSEELQIVDTYDKV
EQVIANVAAVRDAVGPHVGIGVDFHGRVHKPMAKVLAKELDPYKLMFIEEPVLSENAEALRDIVNQTNTPIALGERLYSR
WDFKHILAGGYVDIIQPDASHAGGITECRKIATLAESYDVALALHCPLGPIALAACLQLDAVSYNAFIQEQSLGIHYNQG
NDLLDYLRNPEVFRYEDGFVAIPQGPGLGIDVNEEKVREMAKTGHRWRNPVWRHADGSVAEW

Sequences:

>Translated_382_residues
MKIIRLETFVVPPRWLFLKIETDEGIVGWGEPVVEGRAHTVEAAVQELADYLVGKDPLLIEDHWQVMYRAGFYRGGPIMM
SAIAGVDQALWDIKGKHHGVPVHALLGGQVRDRIKVYSWIGGDRPSDVANNARAVVERGFKAVKMNGSEELQIVDTYDKV
EQVIANVAAVRDAVGPHVGIGVDFHGRVHKPMAKVLAKELDPYKLMFIEEPVLSENAEALRDIVNQTNTPIALGERLYSR
WDFKHILAGGYVDIIQPDASHAGGITECRKIATLAESYDVALALHCPLGPIALAACLQLDAVSYNAFIQEQSLGIHYNQG
NDLLDYLRNPEVFRYEDGFVAIPQGPGLGIDVNEEKVREMAKTGHRWRNPVWRHADGSVAEW
>Mature_382_residues
MKIIRLETFVVPPRWLFLKIETDEGIVGWGEPVVEGRAHTVEAAVQELADYLVGKDPLLIEDHWQVMYRAGFYRGGPIMM
SAIAGVDQALWDIKGKHHGVPVHALLGGQVRDRIKVYSWIGGDRPSDVANNARAVVERGFKAVKMNGSEELQIVDTYDKV
EQVIANVAAVRDAVGPHVGIGVDFHGRVHKPMAKVLAKELDPYKLMFIEEPVLSENAEALRDIVNQTNTPIALGERLYSR
WDFKHILAGGYVDIIQPDASHAGGITECRKIATLAESYDVALALHCPLGPIALAACLQLDAVSYNAFIQEQSLGIHYNQG
NDLLDYLRNPEVFRYEDGFVAIPQGPGLGIDVNEEKVREMAKTGHRWRNPVWRHADGSVAEW

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=382, Percent_Identity=61.5183246073298, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI1787864, Length=413, Percent_Identity=31.4769975786925, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI226510960, Length=268, Percent_Identity=30.9701492537313, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 42421; Mature: 42421

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIRLETFVVPPRWLFLKIETDEGIVGWGEPVVEGRAHTVEAAVQELADYLVGKDPLLI
CEEEEEEEEEECCEEEEEEEECCCCEEECCCHHHCCCHHHHHHHHHHHHHHHHCCCCEEE
EDHWQVMYRAGFYRGGPIMMSAIAGVDQALWDIKGKHHGVPVHALLGGQVRDRIKVYSWI
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHEEEEC
GGDRPSDVANNARAVVERGFKAVKMNGSEELQIVDTYDKVEQVIANVAAVRDAVGPHVGI
CCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCEEE
GVDFHGRVHKPMAKVLAKELDPYKLMFIEEPVLSENAEALRDIVNQTNTPIALGERLYSR
EECCCCCHHHHHHHHHHHHCCCEEEEEEECCHHCCCHHHHHHHHHCCCCCHHHHHHHHHH
WDFKHILAGGYVDIIQPDASHAGGITECRKIATLAESYDVALALHCPLGPIALAACLQLD
CCHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
AVSYNAFIQEQSLGIHYNQGNDLLDYLRNPEVFRYEDGFVAIPQGPGLGIDVNEEKVREM
HCCHHHEEEECCCCEEECCCCHHHHHHCCCCEEEECCCEEECCCCCCCCCCCCHHHHHHH
AKTGHRWRNPVWRHADGSVAEW
HHCCHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIIRLETFVVPPRWLFLKIETDEGIVGWGEPVVEGRAHTVEAAVQELADYLVGKDPLLI
CEEEEEEEEEECCEEEEEEEECCCCEEECCCHHHCCCHHHHHHHHHHHHHHHHCCCCEEE
EDHWQVMYRAGFYRGGPIMMSAIAGVDQALWDIKGKHHGVPVHALLGGQVRDRIKVYSWI
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHEEEEC
GGDRPSDVANNARAVVERGFKAVKMNGSEELQIVDTYDKVEQVIANVAAVRDAVGPHVGI
CCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCEEE
GVDFHGRVHKPMAKVLAKELDPYKLMFIEEPVLSENAEALRDIVNQTNTPIALGERLYSR
EECCCCCHHHHHHHHHHHHCCCEEEEEEECCHHCCCHHHHHHHHHCCCCCHHHHHHHHHH
WDFKHILAGGYVDIIQPDASHAGGITECRKIATLAESYDVALALHCPLGPIALAACLQLD
CCHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
AVSYNAFIQEQSLGIHYNQGNDLLDYLRNPEVFRYEDGFVAIPQGPGLGIDVNEEKVREM
HCCHHHEEEECCCCEEECCCCHHHHHHCCCCEEEECCCEEECCCCCCCCCCCCHHHHHHH
AKTGHRWRNPVWRHADGSVAEW
HHCCHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA