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The map label for this gene is amiC [H]

Identifier: 115352662

GI number: 115352662

Start: 2874174

End: 2875691

Strand: Direct

Name: amiC [H]

Synonym: Bamb_2611

Alternate gene names: 115352662

Gene position: 2874174-2875691 (Clockwise)

Preceding gene: 115352661

Following gene: 115352664

Centisome position: 80.81

GC content: 68.84

Gene sequence:

>1518_bases
ATGTTGATCAAACCGTTCCGCTCGATCGAATCGGCGGCCACCGCGACGCACAACTGGCGGCGCCGCCAGATCCTGCGCGC
GGGCGCGTCGACGCTGGTGCTCGGTCTCGCCGCACCGCGGCTCGCGCACGCGTCGTCGGTGCTCGGCGTGCGCGTGTGGC
CCGCGCGCGATTACACGCGCGTCACGATCGAATCGGACCAGCCGCTGCAGAATAGCCAACAGCTGTTGCAGGGCCCCGAC
CGGCTCGTCGTCGACCTGAGCGGGCTCGATCTCGATCAGGCCCTGCGCGACCTCGTGTCGAAGATCGCGCCGAACGATCC
GCAGATCCAGTCGGTGCGCGTCGGCCAGTACCAGCCGCACGTCGTGCGGATGGTGTTCGACCTGAAAGGCTCGGTGAAGC
CGCAGGTGTTCACGCTGCCGCCGGTGGGCACCTACAAGTACCGGCTCGTGTTCGACCTGTATCCGGCCGTCGCGCCCGAT
CCGCTGTCCGACCTGATCGCGCAGACGGAACGCAAGGAACAGGCGCTCAACGACACCGCGCGCGCGCAGCAAATGCAGCC
GCCGACCTCGCTCGCGGGGCCGGCCGCGCCGTCGGCACCCGCCGCGGGCGACAACACCGACGCGTTCTTCCAGCGCTTCG
CGCAGAACACGCCGGCCACGCCGCGCACGCCGCCGGCCGCCGCGACGCCTTCCACGCCCGCGAAGCCGGCCGTCAAGCCG
CCGCCCGTCATCGCGCGCCGCGACGACAGCGACGACGACGGCGACACCTACAAGTTCACCGCACCGAAATCCGGCAAGGG
CGGCACCATGCGCCTGCTGACGGTCGCGATCGATCCGGGCCACGGCGGCGAGGATCCGGGCGCGATCGGCGGCGGCGGCA
CGTACGAGAAGCACATCGCGCTCGACATCGCGAAGAAGCTGCGCGCGAAGATCGACGGCGCGCCGAACATGCGCGCGATG
ATGACGCGCGACGCCGACTTCTTCGTGCCGCTGAACGTGCGCGTGCAGAAGGCGCGCCGCGTCGGCGCCGACCTGTTCGT
GTCGATCCACGCGGATGCGTTCACGACGCCTTCCGCGCGCGGCTCGTCGGTGTTCGCGCTGTCCGACCACGGCGCGACGA
GCGCCGCGGCACGCTGGATGGCGAACAAGGAAAACTCGTCGGACCTGATCGGCGGCATCAACATCAAGACGCAGGACGCG
GCCGTGAGCCGCGCGCTGTTCGACATGTCGACGACCGCGCAGATCCGCGATTCGCTGCGCTACGGCGGCTACGTGCTGAA
GGAAGTCGGCGGCATCAACAAGCTGCACAAGGGTTCGGTCGAGCAGGCCGGATTCGCGGTGCTGAAGGCACCCGACATTC
CGTCGATCCTCGTCGAGACCGCGTTCATCAGCAACCCGGAAGAAGAGAGCCGGCTGAACGACGAAAGCTATCGCGACCAG
ATGGCCGACGCGATCTTCCGCGGCATCAAGCGTTACTTCGCCGCGAACCCACCGCTCGCGAAGAACCGGATGGCCTGA

Upstream 100 bases:

>100_bases
TCGGCGTGCCCGATCTGGTTTTCTCGCTCGACGTGGACGGCGACGGTCGCGCCCTCACCGTCAGGGCGTTCAGCGCTTCA
GGAAAGGCATGTCTCGAAAG

Downstream 100 bases:

>100_bases
CGCACGGCCCCTCGTCTTCCCCGCCGCCGGCACGCGCTGCGTGCCGGCGCGTCACGACGCCGCGAAGCGGCGCACCAGCT
TCCCGCCGAATACGTTCACC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MLIKPFRSIESAATATHNWRRRQILRAGASTLVLGLAAPRLAHASSVLGVRVWPARDYTRVTIESDQPLQNSQQLLQGPD
RLVVDLSGLDLDQALRDLVSKIAPNDPQIQSVRVGQYQPHVVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAVAPD
PLSDLIAQTERKEQALNDTARAQQMQPPTSLAGPAAPSAPAAGDNTDAFFQRFAQNTPATPRTPPAAATPSTPAKPAVKP
PPVIARRDDSDDDGDTYKFTAPKSGKGGTMRLLTVAIDPGHGGEDPGAIGGGGTYEKHIALDIAKKLRAKIDGAPNMRAM
MTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSARGSSVFALSDHGATSAAARWMANKENSSDLIGGINIKTQDA
AVSRALFDMSTTAQIRDSLRYGGYVLKEVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPEEESRLNDESYRDQ
MADAIFRGIKRYFAANPPLAKNRMA

Sequences:

>Translated_505_residues
MLIKPFRSIESAATATHNWRRRQILRAGASTLVLGLAAPRLAHASSVLGVRVWPARDYTRVTIESDQPLQNSQQLLQGPD
RLVVDLSGLDLDQALRDLVSKIAPNDPQIQSVRVGQYQPHVVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAVAPD
PLSDLIAQTERKEQALNDTARAQQMQPPTSLAGPAAPSAPAAGDNTDAFFQRFAQNTPATPRTPPAAATPSTPAKPAVKP
PPVIARRDDSDDDGDTYKFTAPKSGKGGTMRLLTVAIDPGHGGEDPGAIGGGGTYEKHIALDIAKKLRAKIDGAPNMRAM
MTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSARGSSVFALSDHGATSAAARWMANKENSSDLIGGINIKTQDA
AVSRALFDMSTTAQIRDSLRYGGYVLKEVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPEEESRLNDESYRDQ
MADAIFRGIKRYFAANPPLAKNRMA
>Mature_505_residues
MLIKPFRSIESAATATHNWRRRQILRAGASTLVLGLAAPRLAHASSVLGVRVWPARDYTRVTIESDQPLQNSQQLLQGPD
RLVVDLSGLDLDQALRDLVSKIAPNDPQIQSVRVGQYQPHVVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAVAPD
PLSDLIAQTERKEQALNDTARAQQMQPPTSLAGPAAPSAPAAGDNTDAFFQRFAQNTPATPRTPPAAATPSTPAKPAVKP
PPVIARRDDSDDDGDTYKFTAPKSGKGGTMRLLTVAIDPGHGGEDPGAIGGGGTYEKHIALDIAKKLRAKIDGAPNMRAM
MTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSARGSSVFALSDHGATSAAARWMANKENSSDLIGGINIKTQDA
AVSRALFDMSTTAQIRDSLRYGGYVLKEVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPEEESRLNDESYRDQ
MADAIFRGIKRYFAANPPLAKNRMA

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=486, Percent_Identity=40.3292181069959, Blast_Score=330, Evalue=2e-91,
Organism=Escherichia coli, GI1788776, Length=234, Percent_Identity=47.008547008547, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1790611, Length=229, Percent_Identity=41.0480349344978, Blast_Score=184, Evalue=8e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 54461; Mature: 54461

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIKPFRSIESAATATHNWRRRQILRAGASTLVLGLAAPRLAHASSVLGVRVWPARDYTR
CCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHEEHHHHCCHHHHHCCEEEEEEECCCCCEE
VTIESDQPLQNSQQLLQGPDRLVVDLSGLDLDQALRDLVSKIAPNDPQIQSVRVGQYQPH
EEECCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
VVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAVAPDPLSDLIAQTERKEQALNDTA
HEEEEECCCCCCCCEEEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
RAQQMQPPTSLAGPAAPSAPAAGDNTDAFFQRFAQNTPATPRTPPAAATPSTPAKPAVKP
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
PPVIARRDDSDDDGDTYKFTAPKSGKGGTMRLLTVAIDPGHGGEDPGAIGGGGTYEKHIA
CCEEEECCCCCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHH
LDIAKKLRAKIDGAPNMRAMMTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSAR
HHHHHHHHHHCCCCCCCEEEEECCCCEEEECCHHHHHHHHCCCEEEEEEECCCCCCCCCC
GSSVFALSDHGATSAAARWMANKENSSDLIGGINIKTQDAAVSRALFDMSTTAQIRDSLR
CCEEEEEECCCCHHHHHHHHCCCCCCCCEEECCEEECHHHHHHHHHHHCCHHHHHHHHHH
YGGYVLKEVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPEEESRLNDESYRDQ
HCCCHHHHHCCCCHHCCCCCHHCCEEEEECCCCCHHHHHHHHCCCCHHHHHCCCHHHHHH
MADAIFRGIKRYFAANPPLAKNRMA
HHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLIKPFRSIESAATATHNWRRRQILRAGASTLVLGLAAPRLAHASSVLGVRVWPARDYTR
CCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHEEHHHHCCHHHHHCCEEEEEEECCCCCEE
VTIESDQPLQNSQQLLQGPDRLVVDLSGLDLDQALRDLVSKIAPNDPQIQSVRVGQYQPH
EEECCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
VVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAVAPDPLSDLIAQTERKEQALNDTA
HEEEEECCCCCCCCEEEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
RAQQMQPPTSLAGPAAPSAPAAGDNTDAFFQRFAQNTPATPRTPPAAATPSTPAKPAVKP
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
PPVIARRDDSDDDGDTYKFTAPKSGKGGTMRLLTVAIDPGHGGEDPGAIGGGGTYEKHIA
CCEEEECCCCCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHH
LDIAKKLRAKIDGAPNMRAMMTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSAR
HHHHHHHHHHCCCCCCCEEEEECCCCEEEECCHHHHHHHHCCCEEEEEEECCCCCCCCCC
GSSVFALSDHGATSAAARWMANKENSSDLIGGINIKTQDAAVSRALFDMSTTAQIRDSLR
CCEEEEEECCCCHHHHHHHHCCCCCCCCEEECCEEECHHHHHHHHHHHCCHHHHHHHHHH
YGGYVLKEVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPEEESRLNDESYRDQ
HCCCHHHHHCCCCHHCCCCCHHCCEEEEECCCCCHHHHHHHHCCCCHHHHHCCCHHHHHH
MADAIFRGIKRYFAANPPLAKNRMA
HHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]