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The map label for this gene is rluE [H]

Identifier: 115352624

GI number: 115352624

Start: 2834658

End: 2835236

Strand: Direct

Name: rluE [H]

Synonym: Bamb_2573

Alternate gene names: 115352624

Gene position: 2834658-2835236 (Clockwise)

Preceding gene: 115352621

Following gene: 115352626

Centisome position: 79.7

GC content: 71.33

Gene sequence:

>579_bases
ATGACCCTGATCGCCCTCAACAAGCCGTTCGGCACGATTTGCCAGTTTTCCGCGCACGAGACGCGGCCGTCGCTCGGCGA
CTGGGTAAAAACGCCCGGCGTCTACCCGGCCGGCCGGCTCGACGCGGACAGCGAGGGCCTGCTGCTGCTCACCGACGACG
GCGCGCTGCAGGCGCGCATCGCGGAGCCGCGCCACAAGCTCGTCAAGCGCTACTGGGCGCAGGTCGACGGCGCGCCGGGC
CCGGCCGACCTGAAGGCGCTCGCGCGCGGCGTCGATCTCGGCGACTACGTGACGCGCCCGTGCCGCGCCGAATTCATCGA
GCCGCCCGACGCGCTGTGGCCGCGCAACCCGCCGATCCGCTACCGCGCCGCGATCCCGACCACGTGGATCGAACTGGCGA
TCACCGAAGGCAAGAATCGGCAGGTGCGCCGGATGACGGCCGCGATCGGCTTCCCGACGTTGCGTCTCGTGCGCGTGGGC
ATCGGCGCGCTGGACATATTCGCGCTCGGCATTGCGCCGGGCGAAACAATCGCGCTGCCGCCGCGCGCCCCGTGGGACGG
TTTTGCCCGCGACGCATGA

Upstream 100 bases:

>100_bases
AGACAGATGACTTGCCGTTCCGTATTATGCATTAAGATTCCGCCATTTGCCATAGACCGCCGGCCCGCCTTCCCGCGGCA
CGCCGCGCGGGCGCCCCGCC

Downstream 100 bases:

>100_bases
GTGCGCCGGGCAACTTTCGATTTTTTCGTCATCTTTTTCGTCAACCGCCGGCGAAGATCGCGCGTTAAATCACGCAGATG
CCATCCTGAGCTATCCGGCA

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MTLIALNKPFGTICQFSAHETRPSLGDWVKTPGVYPAGRLDADSEGLLLLTDDGALQARIAEPRHKLVKRYWAQVDGAPG
PADLKALARGVDLGDYVTRPCRAEFIEPPDALWPRNPPIRYRAAIPTTWIELAITEGKNRQVRRMTAAIGFPTLRLVRVG
IGALDIFALGIAPGETIALPPRAPWDGFARDA

Sequences:

>Translated_192_residues
MTLIALNKPFGTICQFSAHETRPSLGDWVKTPGVYPAGRLDADSEGLLLLTDDGALQARIAEPRHKLVKRYWAQVDGAPG
PADLKALARGVDLGDYVTRPCRAEFIEPPDALWPRNPPIRYRAAIPTTWIELAITEGKNRQVRRMTAAIGFPTLRLVRVG
IGALDIFALGIAPGETIALPPRAPWDGFARDA
>Mature_191_residues
TLIALNKPFGTICQFSAHETRPSLGDWVKTPGVYPAGRLDADSEGLLLLTDDGALQARIAEPRHKLVKRYWAQVDGAPGP
ADLKALARGVDLGDYVTRPCRAEFIEPPDALWPRNPPIRYRAAIPTTWIELAITEGKNRQVRRMTAAIGFPTLRLVRVGI
GALDIFALGIAPGETIALPPRAPWDGFARDA

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=170, Percent_Identity=51.1764705882353, Blast_Score=173, Evalue=7e-45,
Organism=Escherichia coli, GI1788510, Length=175, Percent_Identity=33.1428571428571, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1790453, Length=180, Percent_Identity=29.4444444444444, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 20956; Mature: 20825

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLIALNKPFGTICQFSAHETRPSLGDWVKTPGVYPAGRLDADSEGLLLLTDDGALQARI
CEEEEECCCCCHHEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEEC
AEPRHKLVKRYWAQVDGAPGPADLKALARGVDLGDYVTRPCRAEFIEPPDALWPRNPPIR
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHCCCCCHHCCCCCCCCCCCCCCEE
YRAAIPTTWIELAITEGKNRQVRRMTAAIGFPTLRLVRVGIGALDIFALGIAPGETIALP
EEEECCCEEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHCCCHHHEEEEECCCCCEEECC
PRAPWDGFARDA
CCCCCCCCCCCC
>Mature Secondary Structure 
TLIALNKPFGTICQFSAHETRPSLGDWVKTPGVYPAGRLDADSEGLLLLTDDGALQARI
EEEEECCCCCHHEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEEC
AEPRHKLVKRYWAQVDGAPGPADLKALARGVDLGDYVTRPCRAEFIEPPDALWPRNPPIR
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHCCCCCHHCCCCCCCCCCCCCCEE
YRAAIPTTWIELAITEGKNRQVRRMTAAIGFPTLRLVRVGIGALDIFALGIAPGETIALP
EEEECCCEEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHCCCHHHEEEEECCCCCEEECC
PRAPWDGFARDA
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]