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The map label for this gene is rsuA [H]

Identifier: 115352585

GI number: 115352585

Start: 2791983

End: 2792699

Strand: Direct

Name: rsuA [H]

Synonym: Bamb_2534

Alternate gene names: 115352585

Gene position: 2791983-2792699 (Clockwise)

Preceding gene: 115352582

Following gene: 115352586

Centisome position: 78.5

GC content: 70.43

Gene sequence:

>717_bases
ATGGATCTCGAAAGCATTCTCTACACGCAGGGCTTCGGCTCGCGCCGCCAGTGCCGCGGCCTGATCGAAACGGGCCGCGT
CACCGTCGCGGGCGCGAGCGCGACCGACCCCGACGCGTCGTTCGACACCGACGGCCTCGTCTTCACGGTCGACGACACCG
CCTGGCCGTTCCACGCCCGCGCGTATCTGGCGCTCAACAAGCCGGCCGGCTACGAGTGTTCGCGCGACCCGCAACACCAC
GCGAGCGTGTTCAGCCTGCTGCCCGCGCCGCTCGTCGCACGCGGCGTGCAGTGTGTCGGCCGTCTGGACCAGGACACGAC
GGGCTTGCTGCTGCTGTCCGACGACGGCCAGTTCGTGCACGCGTACACGTCGCCCAAGCGCAAGGTGCCGAAGACCTACG
TCGCGACCGTGCGCCATCCGCTCGACGACACCCAGCTGAACGCGCTGCGCGCCGGCGTGCAGCTCCACGGCGAGCCGAAG
CCGATCGCGGCGGTCGCCGCCCACGCGCGCGACACGCACGCGCTCGCGCTGACCGTCCTCGAAGGCAAATACCATCAGGT
GAAACGCATGGTCGCGGCGGCCAGCAACCGCGTCGAGGCGCTGCACCGCGAAAGCATCGGCGGCTTCACGCTGCCGGAGG
ATCTCGCGCCCGGCGCGTGGCGCTGGCTCGGGGAACGCGAGCTGTCGGCGCTGCGCGATCCCGTCAAAACCCTCTAA

Upstream 100 bases:

>100_bases
ACTTTCCGTGAAGAATGACCGCGCACAGAGCACCGGGAGCTTCCCGGTGCGAGCGCGTTCGTGCCGGTGCCGCCCCGCCG
CTTGATAGAATGCGCGCGCT

Downstream 100 bases:

>100_bases
GGGAAAATCCGCACGACCGTTCGGTGTCTGGCCGCATCTCGGCCGCACGATCCCCCGCTATACTGAAATCACAAGCTGTA
TGAGCAGCGATGCAGGGGGA

Product: pseudouridine synthase, Rsu

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MDLESILYTQGFGSRRQCRGLIETGRVTVAGASATDPDASFDTDGLVFTVDDTAWPFHARAYLALNKPAGYECSRDPQHH
ASVFSLLPAPLVARGVQCVGRLDQDTTGLLLLSDDGQFVHAYTSPKRKVPKTYVATVRHPLDDTQLNALRAGVQLHGEPK
PIAAVAAHARDTHALALTVLEGKYHQVKRMVAAASNRVEALHRESIGGFTLPEDLAPGAWRWLGERELSALRDPVKTL

Sequences:

>Translated_238_residues
MDLESILYTQGFGSRRQCRGLIETGRVTVAGASATDPDASFDTDGLVFTVDDTAWPFHARAYLALNKPAGYECSRDPQHH
ASVFSLLPAPLVARGVQCVGRLDQDTTGLLLLSDDGQFVHAYTSPKRKVPKTYVATVRHPLDDTQLNALRAGVQLHGEPK
PIAAVAAHARDTHALALTVLEGKYHQVKRMVAAASNRVEALHRESIGGFTLPEDLAPGAWRWLGERELSALRDPVKTL
>Mature_238_residues
MDLESILYTQGFGSRRQCRGLIETGRVTVAGASATDPDASFDTDGLVFTVDDTAWPFHARAYLALNKPAGYECSRDPQHH
ASVFSLLPAPLVARGVQCVGRLDQDTTGLLLLSDDGQFVHAYTSPKRKVPKTYVATVRHPLDDTQLNALRAGVQLHGEPK
PIAAVAAHARDTHALALTVLEGKYHQVKRMVAAASNRVEALHRESIGGFTLPEDLAPGAWRWLGERELSALRDPVKTL

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=174, Percent_Identity=45.9770114942529, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1787524, Length=242, Percent_Identity=33.4710743801653, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1790453, Length=231, Percent_Identity=26.8398268398268, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 25897; Mature: 25897

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLESILYTQGFGSRRQCRGLIETGRVTVAGASATDPDASFDTDGLVFTVDDTAWPFHAR
CCHHHHHHHCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCEEE
AYLALNKPAGYECSRDPQHHASVFSLLPAPLVARGVQCVGRLDQDTTGLLLLSDDGQFVH
EEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEE
AYTSPKRKVPKTYVATVRHPLDDTQLNALRAGVQLHGEPKPIAAVAAHARDTHALALTVL
EECCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCEECCCCCCHHHHHHHCCCCCEEEEEEE
EGKYHQVKRMVAAASNRVEALHRESIGGFTLPEDLAPGAWRWLGERELSALRDPVKTL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHHCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLESILYTQGFGSRRQCRGLIETGRVTVAGASATDPDASFDTDGLVFTVDDTAWPFHAR
CCHHHHHHHCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCEEE
AYLALNKPAGYECSRDPQHHASVFSLLPAPLVARGVQCVGRLDQDTTGLLLLSDDGQFVH
EEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEE
AYTSPKRKVPKTYVATVRHPLDDTQLNALRAGVQLHGEPKPIAAVAAHARDTHALALTVL
EECCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCEECCCCCCHHHHHHHCCCCCEEEEEEE
EGKYHQVKRMVAAASNRVEALHRESIGGFTLPEDLAPGAWRWLGERELSALRDPVKTL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHHCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]