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The map label for this gene is prfB [H]

Identifier: 115352205

GI number: 115352205

Start: 2366689

End: 2367909

Strand: Direct

Name: prfB [H]

Synonym: Bamb_2154

Alternate gene names: 115352205

Gene position: 2366689-2367909 (Clockwise)

Preceding gene: 115352204

Following gene: 115352206

Centisome position: 66.54

GC content: 63.8

Gene sequence:

>1221_bases
ATGCGCGCCGCGCCGAACGGGCCACGGCGCGCATGCAAGCACACTCCGCGTCACCGGGAAACCCCGCCGATCGGCTATAA
TTCCGCTTTTTTACGAAGCAAGAACAGCGAACGATCATGGAAGCGGAACGTCTCAACGCGATCGAAAGCTCCCTGCTCGA
CCTGCGCACCCGCGCGGGCGAGCTTCGGGGGTATCTTTGACTACGACGCCAAATCTGCGCGTCTGACCGAAGTCAACAAG
GAACTCGAAGACCCGAACGTCTGGAACGATTCGAAGAACGCCCAGGCACTCGGCCGCGAGAAGAAGCTGCTCGAAGGTGT
CGTCACGACGCTCACCGCGCTCGATAGCGACCTGCGCGATGCGCTCGACCTGTTCGAGCTCGCACGCGAGGAAGGCGACG
AAGACACCCTCGTCGCATCCGAAGACGACGCGAAGAAGCTCGAAGCGCGCGTCGGCGACATCGAATTCCGCCGGATGTTC
TCGAACCCGGCCGACCCGAACAACTGCTTCATCGACATCCAGGCCGGCGCCGGCGGCACCGAGGCGTGCGACTGGGCGTC
GATGCTGCTGCGCCAATACCTGCGCTACTGCGAGCGCAAGGGCTTCAAGGCCGAAGTGCTCGAAGAGTCGGACGGCGACG
TCGCCGGCATCAAGAACGCGACGGTCAAGGTCACGGGCGAATACGCATACGGCTTCCTGCGCACCGAAACGGGCATCCAC
CGCCTCGTGCGCAAGTCGCCGTTCGACTCGTCCGGCGGCCGCCACACGTCGTTCTCGTCGGTGTTCGTGTATCCGGAAAT
CGACGACTCGATCGAAGTCGAGGTCAATCCGGCCGACCTGCGCATCGACACGTACCGCGCGTCGGGCGCGGGCGGCCAGC
ACATCAACAAGACCGACTCGGCCGTGCGGATCACGCACATGCCGACCGGCATCGTCGTGCAGTGCCAGAACGACCGCTCG
CAGCACCGCAACCGTGCCGAAGCGATGGCGATGCTGAAGTCGCGCCTGTACGAAGCCGAGATGCGTAAGCGCCAGGCCGA
GCAGGACAAGCTCGAATCGAGCAAGACCGATGTGGGCTGGGGCCACCAGATCCGTTCGTACGTGCTCGACCAGAGCCGCG
TGAAGGACCTGCGCACGAACGTCGAAATGAGCAACACGCGAGCCGTGCTCGACGGCGATCTCGACGACTTCATCAGCGCG
AGCCTCAAACAGGGCGTGTAA

Upstream 100 bases:

>100_bases
TCGTCGCCGACACGTGGAACGGCGTGACGCGCGTCCAGCTGATCGTCGAGCACGCGGCCGGCTGAGCACCGGCCACCCGG
CCCGCGCGCTCGCGGGCCGC

Downstream 100 bases:

>100_bases
GCGCCGCGGCGCGGCCTCTCCGGTCGCGCCGCCCTTCCCGTTGCGTTGCCGCACCCATTCCGAATTCCGACCATCATGAC
CGAACCGACCCAAACGCAGC

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MRAAPNGPRRACKHTPRHRETPPIGYNSAFLRSKNSERSWKRNVSTRSKAPCSTCAPARASFGGIFDYDAKSARLTEVNK
ELEDPNVWNDSKNAQALGREKKLLEGVVTTLTALDSDLRDALDLFELAREEGDEDTLVASEDDAKKLEARVGDIEFRRMF
SNPADPNNCFIDIQAGAGGTEACDWASMLLRQYLRYCERKGFKAEVLEESDGDVAGIKNATVKVTGEYAYGFLRTETGIH
RLVRKSPFDSSGGRHTSFSSVFVYPEIDDSIEVEVNPADLRIDTYRASGAGGQHINKTDSAVRITHMPTGIVVQCQNDRS
QHRNRAEAMAMLKSRLYEAEMRKRQAEQDKLESSKTDVGWGHQIRSYVLDQSRVKDLRTNVEMSNTRAVLDGDLDDFISA
SLKQGV

Sequences:

>Translated_406_residues
MRAAPNGPRRACKHTPRHRETPPIGYNSAFLRSKNSERSWKRNVSTRSKAPCSTCAPARASFGGIFDYDAKSARLTEVNK
ELEDPNVWNDSKNAQALGREKKLLEGVVTTLTALDSDLRDALDLFELAREEGDEDTLVASEDDAKKLEARVGDIEFRRMF
SNPADPNNCFIDIQAGAGGTEACDWASMLLRQYLRYCERKGFKAEVLEESDGDVAGIKNATVKVTGEYAYGFLRTETGIH
RLVRKSPFDSSGGRHTSFSSVFVYPEIDDSIEVEVNPADLRIDTYRASGAGGQHINKTDSAVRITHMPTGIVVQCQNDRS
QHRNRAEAMAMLKSRLYEAEMRKRQAEQDKLESSKTDVGWGHQIRSYVLDQSRVKDLRTNVEMSNTRAVLDGDLDDFISA
SLKQGV
>Mature_406_residues
MRAAPNGPRRACKHTPRHRETPPIGYNSAFLRSKNSERSWKRNVSTRSKAPCSTCAPARASFGGIFDYDAKSARLTEVNK
ELEDPNVWNDSKNAQALGREKKLLEGVVTTLTALDSDLRDALDLFELAREEGDEDTLVASEDDAKKLEARVGDIEFRRMF
SNPADPNNCFIDIQAGAGGTEACDWASMLLRQYLRYCERKGFKAEVLEESDGDVAGIKNATVKVTGEYAYGFLRTETGIH
RLVRKSPFDSSGGRHTSFSSVFVYPEIDDSIEVEVNPADLRIDTYRASGAGGQHINKTDSAVRITHMPTGIVVQCQNDRS
QHRNRAEAMAMLKSRLYEAEMRKRQAEQDKLESSKTDVGWGHQIRSYVLDQSRVKDLRTNVEMSNTRAVLDGDLDDFISA
SLKQGV

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=310, Percent_Identity=34.5161290322581, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI34577120, Length=250, Percent_Identity=37.6, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI166795305, Length=210, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=6e-28,
Organism=Escherichia coli, GI2367172, Length=343, Percent_Identity=63.265306122449, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1787462, Length=246, Percent_Identity=43.4959349593496, Blast_Score=213, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=268, Percent_Identity=32.8358208955224, Blast_Score=112, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=249, Percent_Identity=34.5381526104418, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI19921226, Length=306, Percent_Identity=35.9477124183007, Blast_Score=162, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 45323; Mature: 45323

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAAPNGPRRACKHTPRHRETPPIGYNSAFLRSKNSERSWKRNVSTRSKAPCSTCAPARA
CCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCHH
SFGGIFDYDAKSARLTEVNKELEDPNVWNDSKNAQALGREKKLLEGVVTTLTALDSDLRD
CCCCEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALDLFELAREEGDEDTLVASEDDAKKLEARVGDIEFRRMFSNPADPNNCFIDIQAGAGGT
HHHHHHHHHHCCCCCCEEECCCHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEECCCCCC
EACDWASMLLRQYLRYCERKGFKAEVLEESDGDVAGIKNATVKVTGEYAYGFLRTETGIH
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEECCCCCEEEEEECHHEEEEHHHHHHH
RLVRKSPFDSSGGRHTSFSSVFVYPEIDDSIEVEVNPADLRIDTYRASGAGGQHINKTDS
HHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCC
AVRITHMPTGIVVQCQNDRSQHRNRAEAMAMLKSRLYEAEMRKRQAEQDKLESSKTDVGW
EEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
GHQIRSYVLDQSRVKDLRTNVEMSNTRAVLDGDLDDFISASLKQGV
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRAAPNGPRRACKHTPRHRETPPIGYNSAFLRSKNSERSWKRNVSTRSKAPCSTCAPARA
CCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCHH
SFGGIFDYDAKSARLTEVNKELEDPNVWNDSKNAQALGREKKLLEGVVTTLTALDSDLRD
CCCCEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALDLFELAREEGDEDTLVASEDDAKKLEARVGDIEFRRMFSNPADPNNCFIDIQAGAGGT
HHHHHHHHHHCCCCCCEEECCCHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEECCCCCC
EACDWASMLLRQYLRYCERKGFKAEVLEESDGDVAGIKNATVKVTGEYAYGFLRTETGIH
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEECCCCCEEEEEECHHEEEEHHHHHHH
RLVRKSPFDSSGGRHTSFSSVFVYPEIDDSIEVEVNPADLRIDTYRASGAGGQHINKTDS
HHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCC
AVRITHMPTGIVVQCQNDRSQHRNRAEAMAMLKSRLYEAEMRKRQAEQDKLESSKTDVGW
EEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
GHQIRSYVLDQSRVKDLRTNVEMSNTRAVLDGDLDDFISASLKQGV
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA