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The map label for this gene is lysS

Identifier: 115352206

GI number: 115352206

Start: 2367985

End: 2369511

Strand: Direct

Name: lysS

Synonym: Bamb_2155

Alternate gene names: 115352206

Gene position: 2367985-2369511 (Clockwise)

Preceding gene: 115352205

Following gene: 115352207

Centisome position: 66.58

GC content: 65.88

Gene sequence:

>1527_bases
ATGACCGAACCGACCCAAACGCAGCCCGCCGTCGCGGCGGACGAAAACCAGATCATCGCCGAGCGCCGCGAGAAGCTGCG
CGCCTTGCGCGAGCAAGGCGTCGCCTATCCGAACGATTTCCGGCCGACGCATCACGCCGCCGATCTCCAGGCAACCTTCG
CCGACTCGGACAAGGCCGCGCTCGAAGCGAACCCGGTCGAGGTGTCGGTCGCCGGCCGCATGATGCTCAAGCGCGTGATG
GGCAAGGCGAGCTTCGCGACGGTGCAGGACGGTTCGGGCCAGATCCAGTTCTTCGTGACGCCGAACGACGTCGGCGCGGA
AACCTACGACGCATTCAAGAAGTGGGACCTCGGCGACATCGTCGCCGCCCGCGGCGTGCTGTTCCGCACCAACAAGGGCG
AGCTGTCGGTCCAGTGCAAGGAGCTGCGCCTGCTGTCGAAGGCGCTGCGCCCGCTGCCGGACAAGTTCCACGGCCTCGCC
GACCAGGAAATGCGCTATCGCCAGCGCTACGTCGACCTGATCGTCACGCCGGAAACGCGCGACACGTTCCGCGCGCGCAC
CCGCACCATCGCGTCGATCCGCAAGTTCATGGACAACGCCGACTTCATGGAAGTCGAAACGCCGATGCTGCACCCGATCC
CGGGCGGCGCCGCGGCCAAGCCGTTCGTCACGCATCACAACGCGCTCGACATGCAGATGTTCCTGCGCATCGCGCCGGAG
CTGTACCTGAAGCGCCTGATCGTCGGCGGCTTCGAGCGCGTGTTCGAAATCAACCGCAACTTCCGGAATGAAGGCGTGTC
GCCGCGCCACAACCCGGAATTCACGATGATGGAGTTCTACGCCGCGTACACCGATTACCGCTGGCTGATGGACTTCACCG
AGCAGCTGATCCGCCAGGCGGCGATCGATGCGCTCGGCACCGCGACGATCCAGTACCAGGGCCGCGAACTCGACCTCGCG
AAGCCGTTCCACCGCCTGACGATCACGCAGGCGATCCAGAAGTACGCGCCGCAGTACACCGACGGCCAGCTGTCGGACGA
CGCGTTCCTGCGTACCGAACTGAAGCGCTTCGGCGTCGACGTGTCGCAGCCGGCCTTCCTGAACGCCGGCATCGGCGCGC
TGCAGCTCGCACTGTTCGAGGAAACCGCCGAATCGCAGCTGTGGGAGCCGACCTACATCATCGACTACCCGGTCGAAGTG
TCGCCGCTCGCACGCGCGTCGGACACGACGCCGGGCATCACCGAGCGTTTCGAGCTGTTCATGACGGGCCGCGAGATCGC
GAACGGCTTCTCGGAGCTGAACGATCCGGAAGACCAGGCCGCGCGCTTCAAGAAGCAGGTCGAGCAGAAGGATGCCGGCG
ACGAGGAAGCGATGTTCTTCGACGCCGACTACATCCGCGCGCTCGAACACGGGATGCCGCCGACGGGTGGCTGCGGGATC
GGCATCGACCGTCTCGTGATGCTGCTGACCGACAGCCCGACGATCCGCGACGTGCTGCTGTTCCCGCACCTGCGCCGCGA
AGACTGA

Upstream 100 bases:

>100_bases
CGCGAGCCTCAAACAGGGCGTGTAAGCGCCGCGGCGCGGCCTCTCCGGTCGCGCCGCCCTTCCCGTTGCGTTGCCGCACC
CATTCCGAATTCCGACCATC

Downstream 100 bases:

>100_bases
CGTGCCTGCGTGTGCGCCGCGCCCCGCGGCGCGCACGCGTGCGGTTTGTAACCACGCGTAAATCCCGCCTGCTGGCGTCC
GCCGCCTTTCGATCCTCCCC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MTEPTQTQPAVAADENQIIAERREKLRALREQGVAYPNDFRPTHHAADLQATFADSDKAALEANPVEVSVAGRMMLKRVM
GKASFATVQDGSGQIQFFVTPNDVGAETYDAFKKWDLGDIVAARGVLFRTNKGELSVQCKELRLLSKALRPLPDKFHGLA
DQEMRYRQRYVDLIVTPETRDTFRARTRTIASIRKFMDNADFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLRIAPE
LYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQAAIDALGTATIQYQGRELDLA
KPFHRLTITQAIQKYAPQYTDGQLSDDAFLRTELKRFGVDVSQPAFLNAGIGALQLALFEETAESQLWEPTYIIDYPVEV
SPLARASDTTPGITERFELFMTGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADYIRALEHGMPPTGGCGI
GIDRLVMLLTDSPTIRDVLLFPHLRRED

Sequences:

>Translated_508_residues
MTEPTQTQPAVAADENQIIAERREKLRALREQGVAYPNDFRPTHHAADLQATFADSDKAALEANPVEVSVAGRMMLKRVM
GKASFATVQDGSGQIQFFVTPNDVGAETYDAFKKWDLGDIVAARGVLFRTNKGELSVQCKELRLLSKALRPLPDKFHGLA
DQEMRYRQRYVDLIVTPETRDTFRARTRTIASIRKFMDNADFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLRIAPE
LYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQAAIDALGTATIQYQGRELDLA
KPFHRLTITQAIQKYAPQYTDGQLSDDAFLRTELKRFGVDVSQPAFLNAGIGALQLALFEETAESQLWEPTYIIDYPVEV
SPLARASDTTPGITERFELFMTGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADYIRALEHGMPPTGGCGI
GIDRLVMLLTDSPTIRDVLLFPHLRRED
>Mature_507_residues
TEPTQTQPAVAADENQIIAERREKLRALREQGVAYPNDFRPTHHAADLQATFADSDKAALEANPVEVSVAGRMMLKRVMG
KASFATVQDGSGQIQFFVTPNDVGAETYDAFKKWDLGDIVAARGVLFRTNKGELSVQCKELRLLSKALRPLPDKFHGLAD
QEMRYRQRYVDLIVTPETRDTFRARTRTIASIRKFMDNADFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLRIAPEL
YLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQAAIDALGTATIQYQGRELDLAK
PFHRLTITQAIQKYAPQYTDGQLSDDAFLRTELKRFGVDVSQPAFLNAGIGALQLALFEETAESQLWEPTYIIDYPVEVS
PLARASDTTPGITERFELFMTGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADYIRALEHGMPPTGGCGIG
IDRLVMLLTDSPTIRDVLLFPHLRRED

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=510, Percent_Identity=39.2156862745098, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI194272210, Length=510, Percent_Identity=39.2156862745098, Blast_Score=349, Evalue=3e-96,
Organism=Homo sapiens, GI40789249, Length=330, Percent_Identity=24.2424242424242, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1789256, Length=506, Percent_Identity=58.300395256917, Blast_Score=607, Evalue=1e-175,
Organism=Escherichia coli, GI1790571, Length=506, Percent_Identity=57.5098814229249, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI87082379, Length=328, Percent_Identity=31.4024390243902, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1788173, Length=250, Percent_Identity=24.8, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=531, Percent_Identity=40.8662900188324, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17535927, Length=531, Percent_Identity=40.8662900188324, Blast_Score=358, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI71994340, Length=498, Percent_Identity=42.1686746987952, Blast_Score=357, Evalue=6e-99,
Organism=Caenorhabditis elegans, GI32566633, Length=280, Percent_Identity=24.2857142857143, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=519, Percent_Identity=41.4258188824663, Blast_Score=365, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324256, Length=551, Percent_Identity=31.0344827586207, Blast_Score=200, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6325153, Length=235, Percent_Identity=26.3829787234043, Blast_Score=63, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24640849, Length=519, Percent_Identity=40.4624277456647, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24640851, Length=519, Percent_Identity=40.4624277456647, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24584738, Length=313, Percent_Identity=25.8785942492013, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYK_BURA4 (B1YT20)

Other databases:

- EMBL:   CP001025
- RefSeq:   YP_001808725.1
- ProteinModelPortal:   B1YT20
- SMR:   B1YT20
- GeneID:   6176170
- GenomeReviews:   CP001025_GR
- KEGG:   bac:BamMC406_2028
- HOGENOM:   HBG631383
- OMA:   GHPQMMS
- ProtClustDB:   PRK00484
- BioCyc:   BAMB398577:BAMMC406_2028-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 57520; Mature: 57389

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPTQTQPAVAADENQIIAERREKLRALREQGVAYPNDFRPTHHAADLQATFADSDKAA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCE
LEANPVEVSVAGRMMLKRVMGKASFATVQDGSGQIQFFVTPNDVGAETYDAFKKWDLGDI
EECCCEEEEHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHCCCHHHH
VAARGVLFRTNKGELSVQCKELRLLSKALRPLPDKFHGLADQEMRYRQRYVDLIVTPETR
HHHCCEEEECCCCCEEEHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHEEEEECCCCH
DTFRARTRTIASIRKFMDNADFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLRIAPE
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHH
LYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIDALGTATIQYQGRELDLAKPFHRLTITQAIQKYAPQYTDGQLSDDAFLRTELKRFGVD
HHHHHCCEEEEECCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
VSQPAFLNAGIGALQLALFEETAESQLWEPTYIIDYPVEVSPLARASDTTPGITERFELF
CCCCCHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHH
MTGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADYIRALEHGMPPTGGCGI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCCCC
GIDRLVMLLTDSPTIRDVLLFPHLRRED
CHHHHEEHHCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
TEPTQTQPAVAADENQIIAERREKLRALREQGVAYPNDFRPTHHAADLQATFADSDKAA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCE
LEANPVEVSVAGRMMLKRVMGKASFATVQDGSGQIQFFVTPNDVGAETYDAFKKWDLGDI
EECCCEEEEHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHCCCHHHH
VAARGVLFRTNKGELSVQCKELRLLSKALRPLPDKFHGLADQEMRYRQRYVDLIVTPETR
HHHCCEEEECCCCCEEEHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHEEEEECCCCH
DTFRARTRTIASIRKFMDNADFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLRIAPE
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHH
LYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIDALGTATIQYQGRELDLAKPFHRLTITQAIQKYAPQYTDGQLSDDAFLRTELKRFGVD
HHHHHCCEEEEECCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
VSQPAFLNAGIGALQLALFEETAESQLWEPTYIIDYPVEVSPLARASDTTPGITERFELF
CCCCCHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHH
MTGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADYIRALEHGMPPTGGCGI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCCCC
GIDRLVMLLTDSPTIRDVLLFPHLRRED
CHHHHEEHHCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA