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The map label for this gene is gcl [H]

Identifier: 115351866

GI number: 115351866

Start: 2002770

End: 2004545

Strand: Direct

Name: gcl [H]

Synonym: Bamb_1815

Alternate gene names: 115351866

Gene position: 2002770-2004545 (Clockwise)

Preceding gene: 115351855

Following gene: 115351867

Centisome position: 56.31

GC content: 66.84

Gene sequence:

>1776_bases
ATGGCCAAGATGAGAGCCGTCGACGCAGCCGTACTCGTGCTCGAGAAGGAAGGCATCCAGACCGCGTTCGGCGTGCCGGG
CGCAGCGATCAACCCGTTCTATTCCGCGATGCGCAAGTCGGGCGGCATCAGCCACGTGCTGGCCCGCCACGTCGAAGGTG
CTTCGCACATGGCCGAAGGCTTCACGCGTGCCGCGCCCGGCAACATCGGCGTGTGCATCGGCACGTCGGGCCCCGCCGGC
ACCGACATGATCACCGGCCTGTACTCCGCATCGGCCGACTCGATTCCGATCCTCGCGATCACGGGCCAGGCACCGCGTGC
CCGCCTGTACAAGGAAGACTTCCAGGCCGTCGACATCGAGTCGATCGCGAAGCCCGTCACCAAGTGGGCCGTCACCGTGC
GCGAACCCGCGCTGGTGCCACGCGTGTTCCAGCAGGCGTTCCACCTGATGCGCTCGGGCCGGCCGGGCCCGGTGCTGGTC
GACCTGCCGATCGACGTGCAGCTCGCCGAAATCGAATTCGACATCGACACGTATGAACCGCTGCCGGTCTACAAGCCCGC
GGCGAGCCGCGCGCAGATCGAGAAGGCGCTCGCGATGCTCAACGACGCGGACAAGCCGCTGATCGTGTCGGGCGGCGGCG
TGCTCAACGCGGCGGCCGAAGACTTGCTCGTGCAGTTCGCCGAAACGATCGGCGTGCCGGTGATCCCGACGCTGATGTCG
TGGGGCGCGATTCCCGACGACCACCCGCTGATGGCCGGCATGGTCGGCCTGCAGACGTCGCACCGCTACGGCAACGCGAC
GATGCTCGCGTCCGACTTCGTGCTCGGCATCGGCAACCGCTGGGCGAACCGCCACACCGGCAGCGTCGAGGTTTATACGA
AGGGCCGCAAGTTCGTGCACGTCGACATCGAGCCGACGCAGATCGGCCGCGTGTTCGGCCCGGATCTCGGCATCGTGTCC
GACGCGAAGGCCGCGCTCGAGCTGTTCGTCGCGGTGGCGCAGGAATGGAAGGCCGCCGGCAAGCTGAAGGATCGCAGCGC
GTGGGTGTCCGAGTGCCAGGCGCGCAAGCGCACGCTGCAGCGCAAGACCCACTTCGACAACGTGCCGGTCAAGCCGCAGC
GCGTGTACGAAGAGATGAACAAGGTGTTCGGCCGCGATACGTGCTACGTCAGCACGATCGGCCTGTCGCAGATCGCCGCC
GCGCAGTTCCTGCACGTGTTCAAGGCACGCAACTGGATCAACTGCGGCCAGGCCGGCCCGCTCGGCTGGACGATCCCCGC
CGCGCTCGGCGTGCGCGCGGCCGATCCGACCCGCCCGATCGTCGCGCTGTCCGGCGACTACGACTTCCAGTTCATGATCG
AAGAGCTGGCCGCCGGCGCGCAATTCAAGCTGCCGTACGTGCACGTGGTGGTGAACAACTCGTACCTCGGCCTGATCCGC
CAGGCCCAGCGCGCGTTCGACATGGACTACTGCGTGCAGCTCGCGTTCGACAACGTCAACGCACCGGAGCTGAACGGTTA
TGGCGTCGATCACGTGGCGGTGGCAGAAGGCCTCGGCTGCAAGGCGCTGCGCGTGTCCAAGCCGGAAGAGATCGAGCCGG
CGCTGAAGCAGGCGCAGGCGCTCGCGCAGGAGTTCAGCGTGCCGGTGGTCGTCGAAGTGATCCTCGAGCGCGTGACGAAC
ATTTCGATGGGCACTGAAATCGATGCGATCAACGAGTTCGAGGAACTCGCCGAGAAGGCCGAGCACGCGCCGACCGCGAT
CTCGATGCTCGACTGA

Upstream 100 bases:

>100_bases
GAAATAAGCGACTTCGGCTGATCTTATCAAACCTTTCCTGCATCCTTAAAGTGTTCCCAACAGAGCGATTCGCAAGATCC
ACACATTCAAGGAGACATGC

Downstream 100 bases:

>100_bases
TCCGCACGCCATTTTTTGACTGACCGACCGAAGAGGCTTAGCTCATGCCGAAGTTTGCTGCAAACCTGACCATGCTGTTC
AACGAAGTGCCGTTCCTCGA

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MAKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEGFTRAAPGNIGVCIGTSGPAG
TDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLV
DLPIDVQLAEIEFDIDTYEPLPVYKPAASRAQIEKALAMLNDADKPLIVSGGGVLNAAAEDLLVQFAETIGVPVIPTLMS
WGAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSVEVYTKGRKFVHVDIEPTQIGRVFGPDLGIVS
DAKAALELFVAVAQEWKAAGKLKDRSAWVSECQARKRTLQRKTHFDNVPVKPQRVYEEMNKVFGRDTCYVSTIGLSQIAA
AQFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPTRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIR
QAQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVSKPEEIEPALKQAQALAQEFSVPVVVEVILERVTN
ISMGTEIDAINEFEELAEKAEHAPTAISMLD

Sequences:

>Translated_591_residues
MAKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEGFTRAAPGNIGVCIGTSGPAG
TDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLV
DLPIDVQLAEIEFDIDTYEPLPVYKPAASRAQIEKALAMLNDADKPLIVSGGGVLNAAAEDLLVQFAETIGVPVIPTLMS
WGAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSVEVYTKGRKFVHVDIEPTQIGRVFGPDLGIVS
DAKAALELFVAVAQEWKAAGKLKDRSAWVSECQARKRTLQRKTHFDNVPVKPQRVYEEMNKVFGRDTCYVSTIGLSQIAA
AQFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPTRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIR
QAQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVSKPEEIEPALKQAQALAQEFSVPVVVEVILERVTN
ISMGTEIDAINEFEELAEKAEHAPTAISMLD
>Mature_590_residues
AKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEGFTRAAPGNIGVCIGTSGPAGT
DMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLVD
LPIDVQLAEIEFDIDTYEPLPVYKPAASRAQIEKALAMLNDADKPLIVSGGGVLNAAAEDLLVQFAETIGVPVIPTLMSW
GAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSVEVYTKGRKFVHVDIEPTQIGRVFGPDLGIVSD
AKAALELFVAVAQEWKAAGKLKDRSAWVSECQARKRTLQRKTHFDNVPVKPQRVYEEMNKVFGRDTCYVSTIGLSQIAAA
QFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPTRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIRQ
AQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVSKPEEIEPALKQAQALAQEFSVPVVVEVILERVTNI
SMGTEIDAINEFEELAEKAEHAPTAISMLD

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=570, Percent_Identity=23.5087719298246, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI21361361, Length=597, Percent_Identity=24.6231155778894, Blast_Score=116, Evalue=5e-26,
Organism=Escherichia coli, GI1786717, Length=590, Percent_Identity=79.3220338983051, Blast_Score=981, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=544, Percent_Identity=34.375, Blast_Score=311, Evalue=7e-86,
Organism=Escherichia coli, GI1790104, Length=476, Percent_Identity=32.3529411764706, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1787096, Length=553, Percent_Identity=26.0397830018083, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1788716, Length=477, Percent_Identity=25.3668763102725, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17542570, Length=554, Percent_Identity=25.4512635379061, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17531299, Length=572, Percent_Identity=22.2027972027972, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17531301, Length=572, Percent_Identity=22.2027972027972, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=598, Percent_Identity=29.933110367893, Blast_Score=259, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6320816, Length=564, Percent_Identity=24.645390070922, Blast_Score=109, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=496, Percent_Identity=20.9677419354839, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19922626, Length=572, Percent_Identity=25, Blast_Score=143, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 63863; Mature: 63732

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEG
CCCCHHHCEEEEEEECCCCCHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHH
FTRAAPGNIGVCIGTSGPAGTDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIE
HHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLVDLPIDVQLAEIEFDIDTYEP
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCC
LPVYKPAASRAQIEKALAMLNDADKPLIVSGGGVLNAAAEDLLVQFAETIGVPVIPTLMS
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
WGAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSVEVYTKGRKFVH
CCCCCCCCCHHHHHHHHCCCCCCCCEEEEHHHHHEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAALELFVAVAQEWKAAGKLKDRSAWVSECQARKRTLQ
EECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RKTHFDNVPVKPQRVYEEMNKVFGRDTCYVSTIGLSQIAAAQFLHVFKARNWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
LGWTIPAALGVRAADPTRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIR
CCEEHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEEECCEEEEEECCCHHHHHH
QAQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVSKPEEIEPALKQAQA
HHHHHHCHHHHHHEEECCCCCCCCCCCCCCHHHHHCCCCCCEEECCCCHHHHHHHHHHHH
LAQEFSVPVVVEVILERVTNISMGTEIDAINEFEELAEKAEHAPTAISMLD
HHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCC
>Mature Secondary Structure 
AKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEG
CCCHHHCEEEEEEECCCCCHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHH
FTRAAPGNIGVCIGTSGPAGTDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIE
HHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLVDLPIDVQLAEIEFDIDTYEP
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCC
LPVYKPAASRAQIEKALAMLNDADKPLIVSGGGVLNAAAEDLLVQFAETIGVPVIPTLMS
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
WGAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSVEVYTKGRKFVH
CCCCCCCCCHHHHHHHHCCCCCCCCEEEEHHHHHEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAALELFVAVAQEWKAAGKLKDRSAWVSECQARKRTLQ
EECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RKTHFDNVPVKPQRVYEEMNKVFGRDTCYVSTIGLSQIAAAQFLHVFKARNWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
LGWTIPAALGVRAADPTRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIR
CCEEHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEEECCEEEEEECCCHHHHHH
QAQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVSKPEEIEPALKQAQA
HHHHHHCHHHHHHEEECCCCCCCCCCCCCCHHHHHCCCCCCEEECCCCHHHHHHHHHHHH
LAQEFSVPVVVEVILERVTNISMGTEIDAINEFEELAEKAEHAPTAISMLD
HHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]