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The map label for this gene is anfA [H]

Identifier: 115351464

GI number: 115351464

Start: 1555033

End: 1556424

Strand: Direct

Name: anfA [H]

Synonym: Bamb_1411

Alternate gene names: 115351464

Gene position: 1555033-1556424 (Clockwise)

Preceding gene: 115351463

Following gene: 115351465

Centisome position: 43.72

GC content: 65.09

Gene sequence:

>1392_bases
ATGAGAAACACGCCCGCAATCGCAGAACTCGATCTGTACGTCTGGGAAGGCAAGGCGGACATTGTCGACCGCGTTGCCCG
CTGCATGGCGAGCTTCGACGTCGAAGTGATCCGCGCCGACAATGCGGAAATCTCGCCGGAGCGCGCCGCATTGCGGCCCT
CGCTCGCGATCATCAGCGTGACGATGATCGAAACCGGCGCGGCGTTCCTGCGCGACTGGCAAGCCAACATCGGCATGCCG
GTCGTCTGGGTCGGCGCGGCGCGCGACCACGACGCATCGCAGTATCCGCCCGACTATTCGCACATTCTGCCGCTCGACTT
CACCTGCGCCGAACTGCGCGGGATGATCGGCAAGCTCGTCACGCAGCTGCGCGCGCACGCGGCCGAAACGCTGCAGCCGT
CCGAACTCGTCGCGCACTCGGAGTCGATGCAGGCGCTGCTGCACGAAGTCGACACATTCGCCGACTGCGACACCAACGTG
CTGCTGCACGGCGAAACCGGTGTCGGCAAGGAGCGCATCGCGCAATTGCTCCACCAGAAGCATTCGCGCTATCGCAACGG
CGAATTCGTGCCGGTCAACTGCGGTGCGATCCCCGACGGCCTGTTCGAATCGCTGTTCTTCGGGCATGCGAAAGGTTCGT
TCACCGGCGCAGTCGTCGCGCATAAAGGCTATTTCGAACAGGCGGCGGGCGGCACGCTGTTCCTCGACGAAGTCGGTGAC
CTGCCGTTGTACCAGCAGGTCAAGCTGTTGCGCGTGCTCGAGGACGGCGCCGTGCTGCGCGTTGGCGCGACGTCGCCGAT
CAAGGTCGATTTTCGTCTGGTCGCGGCCAGCAACAAGAAGCTGCCGCAGCTCGTGAAGGACGGCCTGTTCCGCGCGGATC
TGTACTATCGGCTCGCGGTGATCGAGCTGAGCATTCCGTCGCTCGAGGAGCGGGGCGCGGTCGACAAGATCGCGCTGTTC
AAGTCGTTCGTCGCGGAAGTGGTGGGCGACGAGCGGCTCGCGCAGCTGTCCGATCTGCCTTACTGGCTCGCGGACGCGGT
CGCGGACAGCTATTTCCCCGGCAACGTGCGCGAACTGCGCAACCTCGCGGAACGCGTCGGCGTGACGGTGCGGCAGACTG
GCGGCTGGGACGCGGCGCGGCTGCAGCGGCTGGTCGCGCATGCGCGCAACTCGGCGCAGCCGGTGCCGGTGGAAAGCGCG
GCGGAAGTCTTCGTCGACCGAAGCAAGTGGGACATGAACGAGCGCAGCCGCGTGATCGCCGCCCTCGACGCAAACGGCTG
GCGGCGCCAGGATACGGCGCTGCAGCTCGGCATCAGCCGCAAGGTGTTATGGGAAAAGATGCGCAAGTATCAGATATTCG
ACGAAGAGCCCGAGACCCGCGAAAGCGAATAA

Upstream 100 bases:

>100_bases
ATCTGCTTGGCGTCCAGCTCGGTGCCGCCACGGTACATCAGATGTCGCTGACCTGCGGCGCTGCGCAAACTGTCAGTAAT
TAGAGAAGCAAGACCACCCG

Downstream 100 bases:

>100_bases
TTAATGAGATAAAATCGCGCTGCATTACAACGATACGGGCGGGGAATAAAAATTCATGAGCCATATGACGGGGTTGGCGC
GGGCGTGTGTGTTGCTCGCG

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MRNTPAIAELDLYVWEGKADIVDRVARCMASFDVEVIRADNAEISPERAALRPSLAIISVTMIETGAAFLRDWQANIGMP
VVWVGAARDHDASQYPPDYSHILPLDFTCAELRGMIGKLVTQLRAHAAETLQPSELVAHSESMQALLHEVDTFADCDTNV
LLHGETGVGKERIAQLLHQKHSRYRNGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVGD
LPLYQQVKLLRVLEDGAVLRVGATSPIKVDFRLVAASNKKLPQLVKDGLFRADLYYRLAVIELSIPSLEERGAVDKIALF
KSFVAEVVGDERLAQLSDLPYWLADAVADSYFPGNVRELRNLAERVGVTVRQTGGWDAARLQRLVAHARNSAQPVPVESA
AEVFVDRSKWDMNERSRVIAALDANGWRRQDTALQLGISRKVLWEKMRKYQIFDEEPETRESE

Sequences:

>Translated_463_residues
MRNTPAIAELDLYVWEGKADIVDRVARCMASFDVEVIRADNAEISPERAALRPSLAIISVTMIETGAAFLRDWQANIGMP
VVWVGAARDHDASQYPPDYSHILPLDFTCAELRGMIGKLVTQLRAHAAETLQPSELVAHSESMQALLHEVDTFADCDTNV
LLHGETGVGKERIAQLLHQKHSRYRNGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVGD
LPLYQQVKLLRVLEDGAVLRVGATSPIKVDFRLVAASNKKLPQLVKDGLFRADLYYRLAVIELSIPSLEERGAVDKIALF
KSFVAEVVGDERLAQLSDLPYWLADAVADSYFPGNVRELRNLAERVGVTVRQTGGWDAARLQRLVAHARNSAQPVPVESA
AEVFVDRSKWDMNERSRVIAALDANGWRRQDTALQLGISRKVLWEKMRKYQIFDEEPETRESE
>Mature_463_residues
MRNTPAIAELDLYVWEGKADIVDRVARCMASFDVEVIRADNAEISPERAALRPSLAIISVTMIETGAAFLRDWQANIGMP
VVWVGAARDHDASQYPPDYSHILPLDFTCAELRGMIGKLVTQLRAHAAETLQPSELVAHSESMQALLHEVDTFADCDTNV
LLHGETGVGKERIAQLLHQKHSRYRNGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVGD
LPLYQQVKLLRVLEDGAVLRVGATSPIKVDFRLVAASNKKLPQLVKDGLFRADLYYRLAVIELSIPSLEERGAVDKIALF
KSFVAEVVGDERLAQLSDLPYWLADAVADSYFPGNVRELRNLAERVGVTVRQTGGWDAARLQRLVAHARNSAQPVPVESA
AEVFVDRSKWDMNERSRVIAALDANGWRRQDTALQLGISRKVLWEKMRKYQIFDEEPETRESE

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=339, Percent_Identity=37.1681415929204, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI1788550, Length=344, Percent_Identity=35.1744186046512, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1790437, Length=406, Percent_Identity=33.9901477832512, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1790299, Length=335, Percent_Identity=36.4179104477612, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789087, Length=238, Percent_Identity=43.2773109243698, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789233, Length=338, Percent_Identity=35.5029585798817, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI87082152, Length=257, Percent_Identity=42.4124513618677, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1788905, Length=290, Percent_Identity=35.8620689655172, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI87081872, Length=230, Percent_Identity=41.304347826087, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1786524, Length=335, Percent_Identity=35.8208955223881, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI1787583, Length=350, Percent_Identity=33.7142857142857, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1789828, Length=205, Percent_Identity=36.0975609756098, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI87081858, Length=301, Percent_Identity=29.5681063122924, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51420; Mature: 51420

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNTPAIAELDLYVWEGKADIVDRVARCMASFDVEVIRADNAEISPERAALRPSLAIISV
CCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHCCCEEEEEE
TMIETGAAFLRDWQANIGMPVVWVGAARDHDASQYPPDYSHILPLDFTCAELRGMIGKLV
EHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TQLRAHAAETLQPSELVAHSESMQALLHEVDTFADCDTNVLLHGETGVGKERIAQLLHQK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHH
HSRYRNGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVGD
HHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHCCCCEEEECCCCC
LPLYQQVKLLRVLEDGAVLRVGATSPIKVDFRLVAASNKKLPQLVKDGLFRADLYYRLAV
CCHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCCCHHHHHCCCHHHHHEEEEEE
IELSIPSLEERGAVDKIALFKSFVAEVVGDERLAQLSDLPYWLADAVADSYFPGNVRELR
EEEECCCCHHCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHH
NLAERVGVTVRQTGGWDAARLQRLVAHARNSAQPVPVESAAEVFVDRSKWDMNERSRVIA
HHHHHHCEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEE
ALDANGWRRQDTALQLGISRKVLWEKMRKYQIFDEEPETRESE
EECCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRNTPAIAELDLYVWEGKADIVDRVARCMASFDVEVIRADNAEISPERAALRPSLAIISV
CCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHCCCEEEEEE
TMIETGAAFLRDWQANIGMPVVWVGAARDHDASQYPPDYSHILPLDFTCAELRGMIGKLV
EHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TQLRAHAAETLQPSELVAHSESMQALLHEVDTFADCDTNVLLHGETGVGKERIAQLLHQK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHH
HSRYRNGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVGD
HHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHCCCCEEEECCCCC
LPLYQQVKLLRVLEDGAVLRVGATSPIKVDFRLVAASNKKLPQLVKDGLFRADLYYRLAV
CCHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCCCHHHHHCCCHHHHHEEEEEE
IELSIPSLEERGAVDKIALFKSFVAEVVGDERLAQLSDLPYWLADAVADSYFPGNVRELR
EEEECCCCHHCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHH
NLAERVGVTVRQTGGWDAARLQRLVAHARNSAQPVPVESAAEVFVDRSKWDMNERSRVIA
HHHHHHCEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEE
ALDANGWRRQDTALQLGISRKVLWEKMRKYQIFDEEPETRESE
EECCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]