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The map label for this gene is psdht [H]

Identifier: 115351189

GI number: 115351189

Start: 1247748

End: 1248668

Strand: Direct

Name: psdht [H]

Synonym: Bamb_1136

Alternate gene names: 115351189

Gene position: 1247748-1248668 (Clockwise)

Preceding gene: 115351186

Following gene: 115351192

Centisome position: 35.08

GC content: 71.44

Gene sequence:

>921_bases
ATGCCGCTTCATATCCCGACTCCCTATATCCGTTCGCAAATCGCGTCGCGCCGCCTCGGCACGAACATCCGGCTGAAGCT
CGACGCGCTGCAGCCGTCCGGTTCGTTCAAGTTGCGCGGCATCGGCGCGGTTTGCGAAGCGCGGCATGCGGCCGGCGCGC
GCCGGTTCGTGTCGTCGTCGGGCGGCAATGCGGGCATCGGGGTCGCGTATTGCGGCCGTGAACTCGGCGTGCCGGTGCTC
GTCGTCGTGCCGGAAACCGCGTCGGCCCGCGCGCGCGAGCTGATTCGTGTCGAAGGCGCGGAGGTGGTCGTGCACGGCGC
GAGCTGGGTGGAGGCGAACGCGTTCGCGCTGGCGGCCGTCGGCGCGCAGGACGCGTTCGTGCATCCGTTCGACGACCCGG
TGCTGTGGCAAGGGCACGCGACGATGATCGACGAGATGGCGCTGACCGGCCCGAAGCCCGACGCGGTCGTGCTGTCGGTC
GGCGGCGGCGGGCTGCTGTGCGGCGTGCTCGAGGGGCTTGCGCGCAACGGCTGGCACGACGTGCCGGTGGTCGCGGTCGA
GACGGAGGGCGCCGACAGCTATGCGCGCTCGGTCGCGCAGGGGCGCCCGGTCGAGTTGCCGGCGATCACGAGCGTCGCGA
CGACGCTCGGCGCGAAGCGGCCGTGCGATGCGGCGGTCGAATGGGCGACGCGGCATCCGATTCATCCGGTCGTCGTGACG
GATGCGCAGGCGGTGGCGGCGTCGTTGCGGTTCCTGGACGAGCACCGGATCGTCGTCGAGCCGGCGTGCGGCGCGGCGCT
GGCCGCGCTCGAGCAGTCGGTGCCAGTGCTTGCGTCGGCGTCGGACATCGCGGTGATCGTGTGCGGCGGCGTGACGGCGA
CGGTCGAGCAACTGCAGACGTTGAACGCGACGTTGCGGTAA

Upstream 100 bases:

>100_bases
AAGCATGGCCCGCGTGCCGTACGATGGGCGCCATTGCCGGGCCTGTTCACAGGCTGCGTGCGATTCCCTTGTCCCGACTG
TCCTCACGGTGTCCCGCGCC

Downstream 100 bases:

>100_bases
CGCGCTGACGCCCCGGCGTACGTCACGCGGCGCAGCGGGACGACGTTGCAGAAGAAGCCGTAACGGCGCCTGTGCAGTTA
GTTCGCGGCGGGCGCGCTGG

Product: pyridoxal-5'-phosphate-dependent enzyme subunit beta

Products: oxobutanoate; NH3; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MPLHIPTPYIRSQIASRRLGTNIRLKLDALQPSGSFKLRGIGAVCEARHAAGARRFVSSSGGNAGIGVAYCGRELGVPVL
VVVPETASARARELIRVEGAEVVVHGASWVEANAFALAAVGAQDAFVHPFDDPVLWQGHATMIDEMALTGPKPDAVVLSV
GGGGLLCGVLEGLARNGWHDVPVVAVETEGADSYARSVAQGRPVELPAITSVATTLGAKRPCDAAVEWATRHPIHPVVVT
DAQAVAASLRFLDEHRIVVEPACGAALAALEQSVPVLASASDIAVIVCGGVTATVEQLQTLNATLR

Sequences:

>Translated_306_residues
MPLHIPTPYIRSQIASRRLGTNIRLKLDALQPSGSFKLRGIGAVCEARHAAGARRFVSSSGGNAGIGVAYCGRELGVPVL
VVVPETASARARELIRVEGAEVVVHGASWVEANAFALAAVGAQDAFVHPFDDPVLWQGHATMIDEMALTGPKPDAVVLSV
GGGGLLCGVLEGLARNGWHDVPVVAVETEGADSYARSVAQGRPVELPAITSVATTLGAKRPCDAAVEWATRHPIHPVVVT
DAQAVAASLRFLDEHRIVVEPACGAALAALEQSVPVLASASDIAVIVCGGVTATVEQLQTLNATLR
>Mature_305_residues
PLHIPTPYIRSQIASRRLGTNIRLKLDALQPSGSFKLRGIGAVCEARHAAGARRFVSSSGGNAGIGVAYCGRELGVPVLV
VVPETASARARELIRVEGAEVVVHGASWVEANAFALAAVGAQDAFVHPFDDPVLWQGHATMIDEMALTGPKPDAVVLSVG
GGGLLCGVLEGLARNGWHDVPVVAVETEGADSYARSVAQGRPVELPAITSVATTLGAKRPCDAAVEWATRHPIHPVVVTD
AQAVAASLRFLDEHRIVVEPACGAALAALEQSVPVLASASDIAVIVCGGVTATVEQLQTLNATLR

Specific function: Catalyzes The Formation Of Alpha-Ketobutyrate From Threonine In A Two-Step Reaction. The First Step Is A Dehydration Of Threonine, Followed By Rehydration And Liberation Of Ammonia. Deaminates L-Threonine, But Also L-Serine To A Lesser Extent. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI33469958, Length=317, Percent_Identity=45.7413249211356, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI19923959, Length=317, Percent_Identity=43.8485804416404, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI11345492, Length=296, Percent_Identity=27.027027027027, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1790207, Length=275, Percent_Identity=31.6363636363636, Blast_Score=95, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71991565, Length=296, Percent_Identity=28.7162162162162, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17508781, Length=148, Percent_Identity=28.3783783783784, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6319788, Length=332, Percent_Identity=35.2409638554217, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6322024, Length=201, Percent_Identity=38.3084577114428, Blast_Score=141, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320930, Length=298, Percent_Identity=27.1812080536913, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322023, Length=121, Percent_Identity=35.5371900826446, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21355833, Length=295, Percent_Identity=31.1864406779661, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19 [C]

Molecular weight: Translated: 31693; Mature: 31561

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLHIPTPYIRSQIASRRLGTNIRLKLDALQPSGSFKLRGIGAVCEARHAAGARRFVSSS
CCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHCC
GGNAGIGVAYCGRELGVPVLVVVPETASARARELIRVEGAEVVVHGASWVEANAFALAAV
CCCCCEEHEECCHHHCCCEEEEECCCCCHHHHHHHEECCCEEEEECCCEEECCEEEEEEE
GAQDAFVHPFDDPVLWQGHATMIDEMALTGPKPDAVVLSVGGGGLLCGVLEGLARNGWHD
CCCCCEECCCCCCEEECCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCC
VPVVAVETEGADSYARSVAQGRPVELPAITSVATTLGAKRPCDAAVEWATRHPIHPVVVT
CCEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEE
DAQAVAASLRFLDEHRIVVEPACGAALAALEQSVPVLASASDIAVIVCGGVTATVEQLQT
CHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCEEECCCCEEEEEECCCHHHHHHHHH
LNATLR
HHHCCC
>Mature Secondary Structure 
PLHIPTPYIRSQIASRRLGTNIRLKLDALQPSGSFKLRGIGAVCEARHAAGARRFVSSS
CCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHCC
GGNAGIGVAYCGRELGVPVLVVVPETASARARELIRVEGAEVVVHGASWVEANAFALAAV
CCCCCEEHEECCHHHCCCEEEEECCCCCHHHHHHHEECCCEEEEECCCEEECCEEEEEEE
GAQDAFVHPFDDPVLWQGHATMIDEMALTGPKPDAVVLSVGGGGLLCGVLEGLARNGWHD
CCCCCEECCCCCCEEECCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCC
VPVVAVETEGADSYARSVAQGRPVELPAITSVATTLGAKRPCDAAVEWATRHPIHPVVVT
CCEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEE
DAQAVAASLRFLDEHRIVVEPACGAALAALEQSVPVLASASDIAVIVCGGVTATVEQLQT
CHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCEEECCCCEEEEEECCCHHHHHHHHH
LNATLR
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {L-Ser}} 5 {L-Ser}} 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} [C]

Substrates: @THRE01.txt*L-threonine!; H2O [C]

Specific reaction: @THRE01.txt*L-threonine! + H2O = oxobutanoate + NH3 + H2O [C]

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]