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The map label for this gene is bkdA1 [H]

Identifier: 115351176

GI number: 115351176

Start: 1231116

End: 1232348

Strand: Direct

Name: bkdA1 [H]

Synonym: Bamb_1123

Alternate gene names: 115351176

Gene position: 1231116-1232348 (Clockwise)

Preceding gene: 115351175

Following gene: 115351177

Centisome position: 34.62

GC content: 67.64

Gene sequence:

>1233_bases
ATGAGCCTGTCAGAGCCATTGCGTCTGCATGTGCCGGAGCCCACCGGGCGTCCGGGCTGCAAGACGGATTTTTCCTATCT
GCATCTATCGCCGGCCGGCGCGGTTCGCCGCCCGCCGATCGACGTTGCCCCCGCGGACACCGCCCATCTCGCCCGCAGCC
TCGTGCGCGTGCTCGACGACCACGGCGCAGCGCTCGGCCCATGGGCGCCGGACCTCGACGATGCGCGCCTGATCGCCGGG
ATGCGCGCGATGCTGAAAACCCGCATCTTCGACGCGCGCATGATGATCGCGCAGCGTCAAAAGAAAATCTCCTTCTACAT
GGTGAGCCTCGGCGAAGAGGCGATCGGCGCCGCGCACGCGATGGCGCTGCGTCACGGCGACATGTGCTTCCCGACCTATC
GCCAGCAAAGCATCCTGATCGCGCGCGACGTGCCGCTCGAACGGATGATCTGTCAGCTGATGTCGAACGAAGGCGACCCG
CTGAAGGGGCGCCAGCTCCCGGTGATGTACTCGGACCGCGAAGCCGGCTTCTTCTCGATCTCCGGCAATCTCGCGACGCA
GTTCATCCAGGCGGTCGGCTGGGCGATGGCGTCGGCGATCAAGGGCGACACGAAGATCGCGTCCGCATGGATCGGCGACG
GCGCGACCGCGGAAGCCGACTTCCACACCGCGCTGACGTTCGCGCACGTGTATCGCGCGCCGGTCGTGCTCAACGTGGTC
AACAACCAGTGGGCGATCTCGACGTTCCAGGCGATCGCGGGCGGCGAGGGCACCACCTTCGCCGGGCGCGGCGTCGGCTG
CGGCATCGCTTCGCTGCGGGTCGACGGCAACGACTTCCTCGCGATCTACGCGGCGTCGACCTGGGCCGCCGAACGCGCGC
GCCGCAATCTCGGCCCGACGCTGATCGAATGGGTCACCTACCGCGCGGGCGCGCATTCGACGTCGGACGATCCGACGAAA
TACCGGCCGGCCGACGACTGGGCCCATTTCCCGCTCGGCGATCCGATCGCGCGCTTCAAGCAGCATCTGATCGCGAAAGG
CATCTGGTCGGACAGCGCGCACGACGCGCTCACGGCCGAGCTGGAAGCCGAAGTGATCGCCGCGCAGAAGGAAGCGGAGC
AGTTCGGCACGCTGGTCGACGACCGGATTCCGTCGCCGGCCTCGATGTTCGACGACGTGTACAAGGAGCTGCCCGCGCAC
CTGCGCCGTCAGCGCCAGGAACTGGGAGCGTGA

Upstream 100 bases:

>100_bases
CAAGGTTGGCGTGCTTATCCGTGCGCCAGCCCGCGCGTGAGCCGGCAACCGGCCGCGCGTTCCATCAGCGTGAGACACAA
TCGAGCGTGGAGACGACACG

Downstream 100 bases:

>100_bases
TCATGGCGCAACAGGAAACGACGACGGCATCGGCGCAGCCGATGACGATGATCCAGGCGCTGCGCTCCGCGATGGACGTG
ATGCTCGAGCGCGACGGCGA

Product: 3-methyl-2-oxobutanoate dehydrogenase

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MSLSEPLRLHVPEPTGRPGCKTDFSYLHLSPAGAVRRPPIDVAPADTAHLARSLVRVLDDHGAALGPWAPDLDDARLIAG
MRAMLKTRIFDARMMIAQRQKKISFYMVSLGEEAIGAAHAMALRHGDMCFPTYRQQSILIARDVPLERMICQLMSNEGDP
LKGRQLPVMYSDREAGFFSISGNLATQFIQAVGWAMASAIKGDTKIASAWIGDGATAEADFHTALTFAHVYRAPVVLNVV
NNQWAISTFQAIAGGEGTTFAGRGVGCGIASLRVDGNDFLAIYAASTWAAERARRNLGPTLIEWVTYRAGAHSTSDDPTK
YRPADDWAHFPLGDPIARFKQHLIAKGIWSDSAHDALTAELEAEVIAAQKEAEQFGTLVDDRIPSPASMFDDVYKELPAH
LRRQRQELGA

Sequences:

>Translated_410_residues
MSLSEPLRLHVPEPTGRPGCKTDFSYLHLSPAGAVRRPPIDVAPADTAHLARSLVRVLDDHGAALGPWAPDLDDARLIAG
MRAMLKTRIFDARMMIAQRQKKISFYMVSLGEEAIGAAHAMALRHGDMCFPTYRQQSILIARDVPLERMICQLMSNEGDP
LKGRQLPVMYSDREAGFFSISGNLATQFIQAVGWAMASAIKGDTKIASAWIGDGATAEADFHTALTFAHVYRAPVVLNVV
NNQWAISTFQAIAGGEGTTFAGRGVGCGIASLRVDGNDFLAIYAASTWAAERARRNLGPTLIEWVTYRAGAHSTSDDPTK
YRPADDWAHFPLGDPIARFKQHLIAKGIWSDSAHDALTAELEAEVIAAQKEAEQFGTLVDDRIPSPASMFDDVYKELPAH
LRRQRQELGA
>Mature_409_residues
SLSEPLRLHVPEPTGRPGCKTDFSYLHLSPAGAVRRPPIDVAPADTAHLARSLVRVLDDHGAALGPWAPDLDDARLIAGM
RAMLKTRIFDARMMIAQRQKKISFYMVSLGEEAIGAAHAMALRHGDMCFPTYRQQSILIARDVPLERMICQLMSNEGDPL
KGRQLPVMYSDREAGFFSISGNLATQFIQAVGWAMASAIKGDTKIASAWIGDGATAEADFHTALTFAHVYRAPVVLNVVN
NQWAISTFQAIAGGEGTTFAGRGVGCGIASLRVDGNDFLAIYAASTWAAERARRNLGPTLIEWVTYRAGAHSTSDDPTKY
RPADDWAHFPLGDPIARFKQHLIAKGIWSDSAHDALTAELEAEVIAAQKEAEQFGTLVDDRIPSPASMFDDVYKELPAHL
RRQRQELGA

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=357, Percent_Identity=37.5350140056022, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI258645172, Length=358, Percent_Identity=37.4301675977654, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI4885543, Length=314, Percent_Identity=25.4777070063694, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI4505685, Length=352, Percent_Identity=25.2840909090909, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI291084742, Length=299, Percent_Identity=25.752508361204, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI291084744, Length=274, Percent_Identity=25.5474452554745, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI86563357, Length=344, Percent_Identity=38.0813953488372, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI86563355, Length=344, Percent_Identity=38.0813953488372, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17536047, Length=305, Percent_Identity=26.2295081967213, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32564172, Length=305, Percent_Identity=26.2295081967213, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321026, Length=347, Percent_Identity=29.971181556196, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21355903, Length=375, Percent_Identity=34.6666666666667, Blast_Score=198, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24639748, Length=323, Percent_Identity=26.0061919504644, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24639746, Length=263, Percent_Identity=26.2357414448669, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24639744, Length=263, Percent_Identity=26.2357414448669, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28571106, Length=263, Percent_Identity=26.2357414448669, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24639740, Length=263, Percent_Identity=26.2357414448669, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR022593 [H]

Pfam domain/function: PF00676 E1_dh; PF12573 OxoDH_E1alpha_N [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 44747; Mature: 44616

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSEPLRLHVPEPTGRPGCKTDFSYLHLSPAGAVRRPPIDVAPADTAHLARSLVRVLDD
CCCCCCCEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HGAALGPWAPDLDDARLIAGMRAMLKTRIFDARMMIAQRQKKISFYMVSLGEEAIGAAHA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHH
MALRHGDMCFPTYRQQSILIARDVPLERMICQLMSNEGDPLKGRQLPVMYSDREAGFFSI
HHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEE
SGNLATQFIQAVGWAMASAIKGDTKIASAWIGDGATAEADFHTALTFAHVYRAPVVLNVV
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEE
NNQWAISTFQAIAGGEGTTFAGRGVGCGIASLRVDGNDFLAIYAASTWAAERARRNLGPT
CCCCHHHHHHHHHCCCCCEECCCCCCCCEEEEEECCCCEEEEEEEHHHHHHHHHHCCCHH
LIEWVTYRAGAHSTSDDPTKYRPADDWAHFPLGDPIARFKQHLIAKGIWSDSAHDALTAE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
LEAEVIAAQKEAEQFGTLVDDRIPSPASMFDDVYKELPAHLRRQRQELGA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLSEPLRLHVPEPTGRPGCKTDFSYLHLSPAGAVRRPPIDVAPADTAHLARSLVRVLDD
CCCCCCEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HGAALGPWAPDLDDARLIAGMRAMLKTRIFDARMMIAQRQKKISFYMVSLGEEAIGAAHA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHH
MALRHGDMCFPTYRQQSILIARDVPLERMICQLMSNEGDPLKGRQLPVMYSDREAGFFSI
HHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEE
SGNLATQFIQAVGWAMASAIKGDTKIASAWIGDGATAEADFHTALTFAHVYRAPVVLNVV
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEE
NNQWAISTFQAIAGGEGTTFAGRGVGCGIASLRVDGNDFLAIYAASTWAAERARRNLGPT
CCCCHHHHHHHHHCCCCCEECCCCCCCCEEEEEECCCCEEEEEEEHHHHHHHHHHCCCHH
LIEWVTYRAGAHSTSDDPTKYRPADDWAHFPLGDPIARFKQHLIAKGIWSDSAHDALTAE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
LEAEVIAAQKEAEQFGTLVDDRIPSPASMFDDVYKELPAHLRRQRQELGA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]