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The map label for this gene is bkdA2 [H]

Identifier: 115351177

GI number: 115351177

Start: 1232351

End: 1233394

Strand: Direct

Name: bkdA2 [H]

Synonym: Bamb_1124

Alternate gene names: 115351177

Gene position: 1232351-1233394 (Clockwise)

Preceding gene: 115351176

Following gene: 115351178

Centisome position: 34.65

GC content: 67.72

Gene sequence:

>1044_bases
ATGGCGCAACAGGAAACGACGACGGCATCGGCGCAGCCGATGACGATGATCCAGGCGCTGCGCTCCGCGATGGACGTGAT
GCTCGAGCGCGACGGCGACGTGGTCGTGTTCGGGCAGGACGTCGGCTACTTCGGCGGCGTGTTCCGCTGCACAGAAGGGC
TGCAGGCCAAGTACGGCAAGTCGCGCGTGTTCGATGCGCCGATCTCGGAAGGCGGGATCGTCGGCGCGGCGGTCGGGATG
GGCGCGTATGGATTGCGGCCGGTCTGCGAGATCCAGTTCGCGGACTACTTCTACCCGGCGTCCGATCAGATCGTGTCGGA
AGGCGCACGGCTGCGCTATCGCTCCGCGGGCCAGTTCACCGCGCCGATGACGATCCGGATGCCGTGCGGCGGCGGCATCT
ACGGCGGCCAGACGCACAGCCAGAGCCCGGAGGCGATGTTCACGCAGGTCTGCGGGTTGCGCACGGTGATGCCGTCGAAT
CCGTACGACGCGAAAGGGCTGCTGATCGCGTCGATCGAGAACGACGATCCGGTGATCTTCCTCGAGCCGAAACGGCTGTA
CAACGGGCCGTTCGACGGCCATCACGAGCGTCCGGTCACGTCGTGGCTCAAGCATCCGGGCAGCGTGGTGCCGGACGGCT
ATTACACGGTGCCGCTCGATACGGCGGCCGTCGTGCGGCCCGGCAACGACCTGACGGTGCTGACGTACGGCACGACCGTC
CACGTGTCGCTCGCCGCGGCCGAGGAGACCGGCATCGACGCGGAAGTGATCGACCTGCGCACGCTGTGGCCGGTCGACCT
CGACACGATCGTCGCGTCGGTGCGCAAGACCGGCCGCTGCGTAGTGGTGCACGAAGCGACGCGCACCTGCGGCTACGGCG
CGGAGCTGGTATCGCTCGTCCAGGAGCACTGCTTCTACCATCTGGAAGCGCCGGTCGAGCGGACGACGGGCTGGGACACG
CCGTATCCGCACGCGCAGGAATGGGCGTATTTCCCCGGCCCGGCCCGGGTTGGCGAAGCATTGCGACGCGTGATGGAGGC
CTGA

Upstream 100 bases:

>100_bases
TCGACGACCGGATTCCGTCGCCGGCCTCGATGTTCGACGACGTGTACAAGGAGCTGCCCGCGCACCTGCGCCGTCAGCGC
CAGGAACTGGGAGCGTGATC

Downstream 100 bases:

>100_bases
GATGGGGATTCATGTGATCAAGATGCCGGATATCGGCGAAGGGATCGCCGAGGTCGAGCTGGTGGCGTGGCACGTGGAAG
TCGGCCAGACGATCAAGGAA

Product: transketolase, central region

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MAQQETTTASAQPMTMIQALRSAMDVMLERDGDVVVFGQDVGYFGGVFRCTEGLQAKYGKSRVFDAPISEGGIVGAAVGM
GAYGLRPVCEIQFADYFYPASDQIVSEGARLRYRSAGQFTAPMTIRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSN
PYDAKGLLIASIENDDPVIFLEPKRLYNGPFDGHHERPVTSWLKHPGSVVPDGYYTVPLDTAAVVRPGNDLTVLTYGTTV
HVSLAAAEETGIDAEVIDLRTLWPVDLDTIVASVRKTGRCVVVHEATRTCGYGAELVSLVQEHCFYHLEAPVERTTGWDT
PYPHAQEWAYFPGPARVGEALRRVMEA

Sequences:

>Translated_347_residues
MAQQETTTASAQPMTMIQALRSAMDVMLERDGDVVVFGQDVGYFGGVFRCTEGLQAKYGKSRVFDAPISEGGIVGAAVGM
GAYGLRPVCEIQFADYFYPASDQIVSEGARLRYRSAGQFTAPMTIRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSN
PYDAKGLLIASIENDDPVIFLEPKRLYNGPFDGHHERPVTSWLKHPGSVVPDGYYTVPLDTAAVVRPGNDLTVLTYGTTV
HVSLAAAEETGIDAEVIDLRTLWPVDLDTIVASVRKTGRCVVVHEATRTCGYGAELVSLVQEHCFYHLEAPVERTTGWDT
PYPHAQEWAYFPGPARVGEALRRVMEA
>Mature_346_residues
AQQETTTASAQPMTMIQALRSAMDVMLERDGDVVVFGQDVGYFGGVFRCTEGLQAKYGKSRVFDAPISEGGIVGAAVGMG
AYGLRPVCEIQFADYFYPASDQIVSEGARLRYRSAGQFTAPMTIRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSNP
YDAKGLLIASIENDDPVIFLEPKRLYNGPFDGHHERPVTSWLKHPGSVVPDGYYTVPLDTAAVVRPGNDLTVLTYGTTVH
VSLAAAEETGIDAEVIDLRTLWPVDLDTIVASVRKTGRCVVVHEATRTCGYGAELVSLVQEHCFYHLEAPVERTTGWDTP
YPHAQEWAYFPGPARVGEALRRVMEA

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=340, Percent_Identity=45.8823529411765, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI34101272, Length=340, Percent_Identity=45.8823529411765, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI156564403, Length=324, Percent_Identity=35.1851851851852, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI291084858, Length=324, Percent_Identity=33.9506172839506, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17506935, Length=335, Percent_Identity=45.9701492537313, Blast_Score=281, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI17538422, Length=347, Percent_Identity=37.463976945245, Blast_Score=212, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6319698, Length=344, Percent_Identity=34.8837209302326, Blast_Score=210, Evalue=2e-55,
Organism=Drosophila melanogaster, GI160714832, Length=346, Percent_Identity=44.7976878612717, Blast_Score=296, Evalue=1e-80,
Organism=Drosophila melanogaster, GI160714828, Length=346, Percent_Identity=44.7976878612717, Blast_Score=296, Evalue=1e-80,
Organism=Drosophila melanogaster, GI21358145, Length=321, Percent_Identity=38.9408099688474, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24650940, Length=321, Percent_Identity=38.9408099688474, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24650943, Length=93, Percent_Identity=43.010752688172, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24650945, Length=93, Percent_Identity=43.010752688172, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI160714826, Length=73, Percent_Identity=43.8356164383562, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 37765; Mature: 37634

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQQETTTASAQPMTMIQALRSAMDVMLERDGDVVVFGQDVGYFGGVFRCTEGLQAKYGK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHCHHHHHHHHHHHHCCC
SRVFDAPISEGGIVGAAVGMGAYGLRPVCEIQFADYFYPASDQIVSEGARLRYRSAGQFT
CEEEECCCCCCCEEEHHHCCCCCCCCCEEEEEEECEECCCCHHHHHCCCCEEEECCCCEE
APMTIRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSNPYDAKGLLIASIENDDPVIF
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCEEEEEECCCCCEEE
LEPKRLYNGPFDGHHERPVTSWLKHPGSVVPDGYYTVPLDTAAVVRPGNDLTVLTYGTTV
ECCHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCEEEEECCCEEEEECCCCEEEEEECCEE
HVSLAAAEETGIDAEVIDLRTLWPVDLDTIVASVRKTGRCVVVHEATRTCGYGAELVSLV
EEEEEECCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHH
QEHCFYHLEAPVERTTGWDTPYPHAQEWAYFPGPARVGEALRRVMEA
HHHCEEEECCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQQETTTASAQPMTMIQALRSAMDVMLERDGDVVVFGQDVGYFGGVFRCTEGLQAKYGK
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHCHHHHHHHHHHHHCCC
SRVFDAPISEGGIVGAAVGMGAYGLRPVCEIQFADYFYPASDQIVSEGARLRYRSAGQFT
CEEEECCCCCCCEEEHHHCCCCCCCCCEEEEEEECEECCCCHHHHHCCCCEEEECCCCEE
APMTIRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSNPYDAKGLLIASIENDDPVIF
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCEEEEEECCCCCEEE
LEPKRLYNGPFDGHHERPVTSWLKHPGSVVPDGYYTVPLDTAAVVRPGNDLTVLTYGTTV
ECCHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCEEEEECCCEEEEECCCCEEEEEECCEE
HVSLAAAEETGIDAEVIDLRTLWPVDLDTIVASVRKTGRCVVVHEATRTCGYGAELVSLV
EEEEEECCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHH
QEHCFYHLEAPVERTTGWDTPYPHAQEWAYFPGPARVGEALRRVMEA
HHHCEEEECCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3416875; 10426958 [H]