Click here to switch to the map view.

The map label for this gene is trmD

Identifier: 115351001

GI number: 115351001

Start: 1038737

End: 1039531

Strand: Direct

Name: trmD

Synonym: Bamb_0947

Alternate gene names: 115351001

Gene position: 1038737-1039531 (Clockwise)

Preceding gene: 115351000

Following gene: 115351002

Centisome position: 29.21

GC content: 65.03

Gene sequence:

>795_bases
ATGAACCAGGTTACGGAGAGCGCGGTGCAGTTCGACGTCGTCACGCTCTTTCCTGAAATGTTCCGCGCGCTGACCGACTG
GGGTATCACCAGCCGGGCCGTGAAGCAGGAGCGCTTCGGGTTGCGCACCTGGAACCCGCGTGATTTCACGACGGACAACT
ACCGCACGGTCGACGATCGTCCGTATGGTGGTGGTCCGGGCATGGTGATGCTGGCCAAGCCGCTCGAGGCCGCGATCGGC
GCTGCGAAAGCAGCGCAGGCGGAGCAGGGCGTCGCGAGCACGCGTGTGGTGATGATGTCGCCGCAAGGTGCGCCGTTCAC
GCACGAACGTGCGGTGCGGATGGCGCAGGAGCCTGGTGTCATCGTGCTGTGCGGCCGCTACGAGGCGATCGATCAGCGTC
TGCTCGACCGTTGTGTAGACGAGGAAATCAGCCTCGGCGATTTCGTTCTGTCCGGTGGCGAACTGCCGGCGATGGCGATG
ATGGATGCCGTCGTGCGCCTGCTGCCCGGCGTGCTGAACGATGCGCAGTCAGCCGTGCAGGACAGCTTCGTCGACGGGCT
GCTCGATTGTCCGCATTACACGCGCCCCGAGGAATACGAGGGAATGCGCGTGCCTGACGTGCTGCTCGGCGGGCATCATG
CCGAGATCGAGCGGTGGCGCCGCCAGGAAGCATTGAAGAATACGTTGCGGAAGCGGCCGGATCTGATCGTCCGGGCGCGC
CGCGAGAAGTTGTTGAGCCGGGCCGACGAGGCGTGGCTTGCGAACCTCGCACGCGAAGCGAAGAACGCCTCCTGA

Upstream 100 bases:

>100_bases
CGACCACCGACGAACGGCTGATTCCGTTCGTCGGCGTATACGTCAAGACGGTGGATCAGGCGGCACGTCGCATCGTCGTC
GACTGGGAAGCCGATTACTC

Downstream 100 bases:

>100_bases
GGCGGCGTCGCGGGCGGGAAATGGCGGTGCCGTGCATGCGTGCGCGGCGCCTGATGTGAATCCATCCTCTGTCGGGGCCA
GGCCTGGAAGCAGGCGGGCG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MNQVTESAVQFDVVTLFPEMFRALTDWGITSRAVKQERFGLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMLAKPLEAAIG
AAKAAQAEQGVASTRVVMMSPQGAPFTHERAVRMAQEPGVIVLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMAM
MDAVVRLLPGVLNDAQSAVQDSFVDGLLDCPHYTRPEEYEGMRVPDVLLGGHHAEIERWRRQEALKNTLRKRPDLIVRAR
REKLLSRADEAWLANLAREAKNAS

Sequences:

>Translated_264_residues
MNQVTESAVQFDVVTLFPEMFRALTDWGITSRAVKQERFGLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMLAKPLEAAIG
AAKAAQAEQGVASTRVVMMSPQGAPFTHERAVRMAQEPGVIVLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMAM
MDAVVRLLPGVLNDAQSAVQDSFVDGLLDCPHYTRPEEYEGMRVPDVLLGGHHAEIERWRRQEALKNTLRKRPDLIVRAR
REKLLSRADEAWLANLAREAKNAS
>Mature_264_residues
MNQVTESAVQFDVVTLFPEMFRALTDWGITSRAVKQERFGLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMLAKPLEAAIG
AAKAAQAEQGVASTRVVMMSPQGAPFTHERAVRMAQEPGVIVLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMAM
MDAVVRLLPGVLNDAQSAVQDSFVDGLLDCPHYTRPEEYEGMRVPDVLLGGHHAEIERWRRQEALKNTLRKRPDLIVRAR
REKLLSRADEAWLANLAREAKNAS

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=224, Percent_Identity=54.9107142857143, Blast_Score=252, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 29439; Mature: 29439

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQVTESAVQFDVVTLFPEMFRALTDWGITSRAVKQERFGLRTWNPRDFTTDNYRTVDDR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCC
PYGGGPGMVMLAKPLEAAIGAAKAAQAEQGVASTRVVMMSPQGAPFTHERAVRMAQEPGV
CCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCE
IVLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMAMMDAVVRLLPGVLNDAQSAVQ
EEEECCHHHHHHHHHHHHHHCCCCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DSFVDGLLDCPHYTRPEEYEGMRVPDVLLGGHHAEIERWRRQEALKNTLRKRPDLIVRAR
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
REKLLSRADEAWLANLAREAKNAS
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNQVTESAVQFDVVTLFPEMFRALTDWGITSRAVKQERFGLRTWNPRDFTTDNYRTVDDR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCC
PYGGGPGMVMLAKPLEAAIGAAKAAQAEQGVASTRVVMMSPQGAPFTHERAVRMAQEPGV
CCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCE
IVLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMAMMDAVVRLLPGVLNDAQSAVQ
EEEECCHHHHHHHHHHHHHHCCCCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DSFVDGLLDCPHYTRPEEYEGMRVPDVLLGGHHAEIERWRRQEALKNTLRKRPDLIVRAR
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
REKLLSRADEAWLANLAREAKNAS
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA