Click here to switch to the map view.

The map label for this gene is fcl [H]

Identifier: 115350801

GI number: 115350801

Start: 826458

End: 827393

Strand: Direct

Name: fcl [H]

Synonym: Bamb_0747

Alternate gene names: 115350801

Gene position: 826458-827393 (Clockwise)

Preceding gene: 115350800

Following gene: 115350802

Centisome position: 23.24

GC content: 54.59

Gene sequence:

>936_bases
ATGGCGGGATCTGAAGAAAGCGTATTGATCACGGGCGCCGGTGGAGTGATCGGCCACGCATTAAAGCAGGAATTGGCGGA
CTCGGGCTACTCGAACGTCGTCGCAATCACGAGCAGTGATATCGATCTTAGAGATCAGAGCGCGACTGAAAAAATGTTCG
ATGAATTGCGCCCGACTATCGTTTTCCATATGGCGGCGCGGGTGTACGGCATCATGGGGAATATGTCCAATCGTGGCATC
GCGTATCTCGACAACGTTCGAATCAACACCAATGTAGTCGAGGCTGCACGTCAGACAGGGTGCAAGAAGTTTGTCGCCAT
GGGCTCGACAGCCATTTACTCTGATCAGGTCCGTCTCCCGATGTCGGAGGAGCAAATCTGGGTTGGGGCGCCGCATCATT
CCGAGGCTCCGTACGCCCACAGCAAGCGCGGTATGCTTGCGCAGCTCGAGGCCTACAAAGATCAATACGGAATGGACTAC
GCATTTTGCGTATCCACCAATCTCTTTGGTCCGCACGACAAGTTCGACGAGAAATTCGGCCATGTCATTCCGTCGCTTGT
GTCGAAGTTCTATCGTGCATCTGTTCTAGGGCAGCCCATTTCCGTCTGGGGGTCTGGGAAGGCTGAACGGGATTTTCTCT
TCAGTGGCGACGCTGCGTACGCGCTTCGTCTTATCGCCGAAAATCACACGGGCGCAATCAATCTGGCGACCGGTCAATCG
CACACGATCCGGCATACCGTCGACACGCTTTGCCAGATTTCGGGCTTCAGCGGCAGCGTGGAATGGGATGCGACAAAGCC
GGATGGCCAAAAATTGCGTGCATACGATATCTCGCGTCTGACGGCTTTGGGCTTCAAGCCACGATTCTCGTTCGACGAAG
CGTTGGCGATTACTTACGACTGGTATTGCAAGAACGTCGCGTCTGCCAGAAAGTGA

Upstream 100 bases:

>100_bases
CTGACGTTTGCAGCATCACGTTCGATCCATCGTTGAACTCGATTGCGGTCGCGAAGTGTTTGACGGGGAAGCAAATGGTA
AAAATTTGGAGAATTTAGGA

Downstream 100 bases:

>100_bases
TTGCATGGCATTGCTCCCGGTGTGCTTGCGCGTAAATGGGCCGCACACCATCTCTCTAACGTGTGGTCATTCGAGTTGTT
TGATCGATGAAATTTGTATC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MAGSEESVLITGAGGVIGHALKQELADSGYSNVVAITSSDIDLRDQSATEKMFDELRPTIVFHMAARVYGIMGNMSNRGI
AYLDNVRINTNVVEAARQTGCKKFVAMGSTAIYSDQVRLPMSEEQIWVGAPHHSEAPYAHSKRGMLAQLEAYKDQYGMDY
AFCVSTNLFGPHDKFDEKFGHVIPSLVSKFYRASVLGQPISVWGSGKAERDFLFSGDAAYALRLIAENHTGAINLATGQS
HTIRHTVDTLCQISGFSGSVEWDATKPDGQKLRAYDISRLTALGFKPRFSFDEALAITYDWYCKNVASARK

Sequences:

>Translated_311_residues
MAGSEESVLITGAGGVIGHALKQELADSGYSNVVAITSSDIDLRDQSATEKMFDELRPTIVFHMAARVYGIMGNMSNRGI
AYLDNVRINTNVVEAARQTGCKKFVAMGSTAIYSDQVRLPMSEEQIWVGAPHHSEAPYAHSKRGMLAQLEAYKDQYGMDY
AFCVSTNLFGPHDKFDEKFGHVIPSLVSKFYRASVLGQPISVWGSGKAERDFLFSGDAAYALRLIAENHTGAINLATGQS
HTIRHTVDTLCQISGFSGSVEWDATKPDGQKLRAYDISRLTALGFKPRFSFDEALAITYDWYCKNVASARK
>Mature_310_residues
AGSEESVLITGAGGVIGHALKQELADSGYSNVVAITSSDIDLRDQSATEKMFDELRPTIVFHMAARVYGIMGNMSNRGIA
YLDNVRINTNVVEAARQTGCKKFVAMGSTAIYSDQVRLPMSEEQIWVGAPHHSEAPYAHSKRGMLAQLEAYKDQYGMDYA
FCVSTNLFGPHDKFDEKFGHVIPSLVSKFYRASVLGQPISVWGSGKAERDFLFSGDAAYALRLIAENHTGAINLATGQSH
TIRHTVDTLCQISGFSGSVEWDATKPDGQKLRAYDISRLTALGFKPRFSFDEALAITYDWYCKNVASARK

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=312, Percent_Identity=33.974358974359, Blast_Score=183, Evalue=1e-46,
Organism=Homo sapiens, GI7657641, Length=335, Percent_Identity=22.3880597014925, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI42516563, Length=316, Percent_Identity=24.0506329113924, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788365, Length=314, Percent_Identity=33.7579617834395, Blast_Score=188, Evalue=3e-49,
Organism=Escherichia coli, GI48994969, Length=347, Percent_Identity=23.0547550432277, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788353, Length=355, Percent_Identity=23.3802816901408, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17554428, Length=314, Percent_Identity=31.8471337579618, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI19922778, Length=322, Percent_Identity=30.1242236024845, Blast_Score=157, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 34144; Mature: 34013

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSEESVLITGAGGVIGHALKQELADSGYSNVVAITSSDIDLRDQSATEKMFDELRPTI
CCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHH
VFHMAARVYGIMGNMSNRGIAYLDNVRINTNVVEAARQTGCKKFVAMGSTAIYSDQVRLP
HHHHHHHHHHHHCCCCCCCEEEEECEEECHHHHHHHHHHHHHHHHHCCCCEEECCCEECC
MSEEQIWVGAPHHSEAPYAHSKRGMLAQLEAYKDQYGMDYAFCVSTNLFGPHDKFDEKFG
CCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
HVIPSLVSKFYRASVLGQPISVWGSGKAERDFLFSGDAAYALRLIAENHTGAINLATGQS
HHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEECCCHHHEEEEEECCCCCEEEEECCCC
HTIRHTVDTLCQISGFSGSVEWDATKPDGQKLRAYDISRLTALGFKPRFSFDEALAITYD
CHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCEEEEEEH
WYCKNVASARK
HHHHHHHHCCC
>Mature Secondary Structure 
AGSEESVLITGAGGVIGHALKQELADSGYSNVVAITSSDIDLRDQSATEKMFDELRPTI
CCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHH
VFHMAARVYGIMGNMSNRGIAYLDNVRINTNVVEAARQTGCKKFVAMGSTAIYSDQVRLP
HHHHHHHHHHHHCCCCCCCEEEEECEEECHHHHHHHHHHHHHHHHHCCCCEEECCCEECC
MSEEQIWVGAPHHSEAPYAHSKRGMLAQLEAYKDQYGMDYAFCVSTNLFGPHDKFDEKFG
CCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
HVIPSLVSKFYRASVLGQPISVWGSGKAERDFLFSGDAAYALRLIAENHTGAINLATGQS
HHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEECCCHHHEEEEEECCCCCEEEEECCCC
HTIRHTVDTLCQISGFSGSVEWDATKPDGQKLRAYDISRLTALGFKPRFSFDEALAITYD
CHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCEEEEEEH
WYCKNVASARK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]