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The map label for this gene is argT [H]

Identifier: 115350778

GI number: 115350778

Start: 806420

End: 807199

Strand: Direct

Name: argT [H]

Synonym: Bamb_0724

Alternate gene names: 115350778

Gene position: 806420-807199 (Clockwise)

Preceding gene: 115350773

Following gene: 115350779

Centisome position: 22.67

GC content: 67.31

Gene sequence:

>780_bases
ATGAACTGGAAGCTTTCCCTCTGCGCCGTCGCGGCGCTCGCGTGCGCGGCCGTCACGGCCCACGCGGAACAGACCACGCT
GCGCTTCGGGATCGAAGCCGCCTACCCGCCGTTCGAGAGCAAGTCGCCGGCCGGCCAGCTGCAGGGCTTCGACGTCGACA
TCGGCAACGCGGTGTGCGCGAAGCTGAACATGAAGTGCGTGTGGGTCGAGAATGCGTTCGACGGGTTGATCCCGGCGCTG
CAGGCGCGCAAGTTCGACGCGATCAATTCGGCGATGAACATCACCGCGAAGCGCAAGCAGAGCATCGACTTCACGCCGGC
GATCTACGTCGTGCCGATCGTGATGGTCGCGCACCGCGGCTCGCCGCTGCGGCCCGATGCCGCGAGCCTGCGCGGCAAGC
ACGTCGGCGTGCTGCAGGGCTCGTCGCAGGAGGATTTCCTGAAGGCACACTGGGCGAATGCGGGCGTGGCCGTCGTGTCC
TATCCGGACCAGGACCAGATCTACGCCGATCTCGTCGCCGGGCGTCTCGACGCGGCCGTGCAGGAAGCGCAGACCGCGCA
GGACGGCTTCCTCGACAAGCCGGCCGGCCGCGACTACCAGATCGTCGGCGAGCCGCTGAAGGATCCCGCGACGCTCGGCG
AAGGCACCGGCTTCGGGATGCGCAAGGGCGACAAGGCGCTGCAGACTAAGATCGCCGGCGCGCTCGACGCACTGAAGAAG
GACGGCACGCTGAGCGCGCTGTCGCAGAAGTACTTCAAGCGCGACATCGTCGCGAAGTAA

Upstream 100 bases:

>100_bases
CGGCTGCATCGCAAGCCGATCCGGCGCGGGCGCAGCGCCCGGCAACGGCGTCGCGCGACCGGTCTTCATCGCCTCAATCC
GGAATCGAAGGACATCCGTC

Downstream 100 bases:

>100_bases
GCCTGGCCGCGCGCCGGCCGCCGTGCCGGCGCGCGTCGCACCGAATCTCGACGCAGTACGGGGAAGCATGGATTTCGACG
TCATCGTTCTGGGGGCCGGC

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MNWKLSLCAVAALACAAVTAHAEQTTLRFGIEAAYPPFESKSPAGQLQGFDVDIGNAVCAKLNMKCVWVENAFDGLIPAL
QARKFDAINSAMNITAKRKQSIDFTPAIYVVPIVMVAHRGSPLRPDAASLRGKHVGVLQGSSQEDFLKAHWANAGVAVVS
YPDQDQIYADLVAGRLDAAVQEAQTAQDGFLDKPAGRDYQIVGEPLKDPATLGEGTGFGMRKGDKALQTKIAGALDALKK
DGTLSALSQKYFKRDIVAK

Sequences:

>Translated_259_residues
MNWKLSLCAVAALACAAVTAHAEQTTLRFGIEAAYPPFESKSPAGQLQGFDVDIGNAVCAKLNMKCVWVENAFDGLIPAL
QARKFDAINSAMNITAKRKQSIDFTPAIYVVPIVMVAHRGSPLRPDAASLRGKHVGVLQGSSQEDFLKAHWANAGVAVVS
YPDQDQIYADLVAGRLDAAVQEAQTAQDGFLDKPAGRDYQIVGEPLKDPATLGEGTGFGMRKGDKALQTKIAGALDALKK
DGTLSALSQKYFKRDIVAK
>Mature_259_residues
MNWKLSLCAVAALACAAVTAHAEQTTLRFGIEAAYPPFESKSPAGQLQGFDVDIGNAVCAKLNMKCVWVENAFDGLIPAL
QARKFDAINSAMNITAKRKQSIDFTPAIYVVPIVMVAHRGSPLRPDAASLRGKHVGVLQGSSQEDFLKAHWANAGVAVVS
YPDQDQIYADLVAGRLDAAVQEAQTAQDGFLDKPAGRDYQIVGEPLKDPATLGEGTGFGMRKGDKALQTKIAGALDALKK
DGTLSALSQKYFKRDIVAK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=252, Percent_Identity=44.8412698412698, Blast_Score=240, Evalue=5e-65,
Organism=Escherichia coli, GI1788648, Length=231, Percent_Identity=45.4545454545455, Blast_Score=228, Evalue=3e-61,
Organism=Escherichia coli, GI1787088, Length=245, Percent_Identity=32.2448979591837, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1787085, Length=246, Percent_Identity=32.1138211382114, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1788228, Length=263, Percent_Identity=29.277566539924, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787031, Length=247, Percent_Identity=29.5546558704453, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27647; Mature: 27647

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWKLSLCAVAALACAAVTAHAEQTTLRFGIEAAYPPFESKSPAGQLQGFDVDIGNAVCA
CCCCHHHHHHHHHHHHHHHHHHHHHHEEECCEECCCCCCCCCCCCCCCCCEEECCCHHHH
KLNMKCVWVENAFDGLIPALQARKFDAINSAMNITAKRKQSIDFTPAIYVVPIVMVAHRG
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
SPLRPDAASLRGKHVGVLQGSSQEDFLKAHWANAGVAVVSYPDQDQIYADLVAGRLDAAV
CCCCCCHHHHCCCEEEEEECCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHH
QEAQTAQDGFLDKPAGRDYQIVGEPLKDPATLGEGTGFGMRKGDKALQTKIAGALDALKK
HHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
DGTLSALSQKYFKRDIVAK
CCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNWKLSLCAVAALACAAVTAHAEQTTLRFGIEAAYPPFESKSPAGQLQGFDVDIGNAVCA
CCCCHHHHHHHHHHHHHHHHHHHHHHEEECCEECCCCCCCCCCCCCCCCCEEECCCHHHH
KLNMKCVWVENAFDGLIPALQARKFDAINSAMNITAKRKQSIDFTPAIYVVPIVMVAHRG
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
SPLRPDAASLRGKHVGVLQGSSQEDFLKAHWANAGVAVVSYPDQDQIYADLVAGRLDAAV
CCCCCCHHHHCCCEEEEEECCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHH
QEAQTAQDGFLDKPAGRDYQIVGEPLKDPATLGEGTGFGMRKGDKALQTKIAGALDALKK
HHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
DGTLSALSQKYFKRDIVAK
CCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]