Click here to switch to the map view.

The map label for this gene is puuB [H]

Identifier: 115350614

GI number: 115350614

Start: 627227

End: 628648

Strand: Direct

Name: puuB [H]

Synonym: Bamb_0560

Alternate gene names: 115350614

Gene position: 627227-628648 (Clockwise)

Preceding gene: 115350612

Following gene: 115350616

Centisome position: 17.64

GC content: 68.42

Gene sequence:

>1422_bases
ATGACCACCTACAACAAACCCCTGATCCAGGGCGAATCGCGTTTCGACCAGACGCAACTGAACATCGAAAAGGACAACTG
GAACTGGAGCGAGCCGTCCCGCTACGACGGCGAGCGTCCGGCCAACTGGTACGAGGCGTCGCTGTCGCGCCACGAAAACA
GTGCGCCGCTCGCGCACGACGCCGTGTGCGACGTGCTGGTGATCGGCTCCGGGCTGCTCGGCGCATCGGCCGCGCTGCAT
CTCGCGGAAGCGGGCGTCGACACGATCCTCGTCGACAAGCATCACGTCGGCTCCGCGGCGTCGGGCCGCAACGGCGGCCA
GCTCACGCCGGGCCTCGCGCGCTGGGAAGCCGCCGACATGATCGACTACCTGTCGCACGACGATGCGAAGCGGCTGTGGC
GCTTCGCATCGACCGAATCGATGGACCTGATCGATGAAATCGGCGCGCGTTACGCGCTCGACCTCGATCGCAAGCGCGGC
CACGTGACGGCGGCGGTCCACCCGGGCCACATGAGCGCGCTGCTCGACGGCGCGGACGCCCGCCGCCATCTCGGCGACGC
AGGCGTGACGCTCGTCGGCCGCCATCAGCTGCACGACGAATATGTGCGCTCGGGCCTGTATCACGGCGCGGCGATCGACG
CGCTCGGCGGCCAGCTTCATCCACTCGCGCTGGTGCGCGGCCTCGTGCACGGCTTCCAGTTGAACGGCGGCGCGCTGTAC
GAGGGCACCGAGGTGCTCGAGCTCGACGAAACGCCCGCGGGCGTCGTCGCGACGACGCCGGGCGGCACGATCACCGCGCG
TCGCGGCGTCGTGCTCGCGCTGCACAACACGACGTTCCGGCTGCTGGACGACGGCGGCGCGACCACCGTGCCGTTCTTCA
CGTATGTGAGCGTGACGGCGCCGCTCGACGTCGATGTCGCGACGCTGATGCCGGCCGGCATGCCGGTGTACGACACGCAG
TTCCAGATCGACTACTACCGGCCGGTGCGCGGCAACCGGCTGCTGTTCGGCGGGCAGGGCACCGGCTCGTGCTGGCCGCA
GCAGGACGTCAACGCGTATCTGCTGACGCGGCTGAATACCGTGTTCCCGCAGCACGACGGCCGCTTCGCGCTCGACTACT
GCTGGAGCGGCGTCAGCGACTTCACGCTGAACGGCGCGACCGACAGCCGCAAGACCGACGGCCGCGTGCCGATGTACATG
GTGCAGGGCTGGAGCGGGCACGGCGTCGCGCAGACGGTGCGGATCGGCAAGGCGATCTGCGACGATTTCGTCGGACGCAA
CGACGACTTCTCGATGCTGACCGGCATCGACCATCGCGAGATTCCGCTCGGCCGGCAGTTGTCGCCGATCGCGATTCCGG
CGGCGAAAGCGGCGATGAGTGTGATGAGCGCGCTGAATCCGGGGCGGATGATCTCGTTCTGA

Upstream 100 bases:

>100_bases
CGCGCCCTTTATCTCGTTGACACCCAAGTGGAACCGGCTGCCCCACCCGCGAAGCCTCACGGCCCTTCGACTGGCGGCCC
AAGACCATGCAAGTGCAATC

Downstream 100 bases:

>100_bases
GGTGCCGGTTTTCCGACGCGCTGGCCGCGCGCCGGCCGCCAACGAAAAAGCCCGATGCGAACTTGCTCGCATCGGGCTTT
TTTTCCGTCCGTTTTCCGTC

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MTTYNKPLIQGESRFDQTQLNIEKDNWNWSEPSRYDGERPANWYEASLSRHENSAPLAHDAVCDVLVIGSGLLGASAALH
LAEAGVDTILVDKHHVGSAASGRNGGQLTPGLARWEAADMIDYLSHDDAKRLWRFASTESMDLIDEIGARYALDLDRKRG
HVTAAVHPGHMSALLDGADARRHLGDAGVTLVGRHQLHDEYVRSGLYHGAAIDALGGQLHPLALVRGLVHGFQLNGGALY
EGTEVLELDETPAGVVATTPGGTITARRGVVLALHNTTFRLLDDGGATTVPFFTYVSVTAPLDVDVATLMPAGMPVYDTQ
FQIDYYRPVRGNRLLFGGQGTGSCWPQQDVNAYLLTRLNTVFPQHDGRFALDYCWSGVSDFTLNGATDSRKTDGRVPMYM
VQGWSGHGVAQTVRIGKAICDDFVGRNDDFSMLTGIDHREIPLGRQLSPIAIPAAKAAMSVMSALNPGRMISF

Sequences:

>Translated_473_residues
MTTYNKPLIQGESRFDQTQLNIEKDNWNWSEPSRYDGERPANWYEASLSRHENSAPLAHDAVCDVLVIGSGLLGASAALH
LAEAGVDTILVDKHHVGSAASGRNGGQLTPGLARWEAADMIDYLSHDDAKRLWRFASTESMDLIDEIGARYALDLDRKRG
HVTAAVHPGHMSALLDGADARRHLGDAGVTLVGRHQLHDEYVRSGLYHGAAIDALGGQLHPLALVRGLVHGFQLNGGALY
EGTEVLELDETPAGVVATTPGGTITARRGVVLALHNTTFRLLDDGGATTVPFFTYVSVTAPLDVDVATLMPAGMPVYDTQ
FQIDYYRPVRGNRLLFGGQGTGSCWPQQDVNAYLLTRLNTVFPQHDGRFALDYCWSGVSDFTLNGATDSRKTDGRVPMYM
VQGWSGHGVAQTVRIGKAICDDFVGRNDDFSMLTGIDHREIPLGRQLSPIAIPAAKAAMSVMSALNPGRMISF
>Mature_472_residues
TTYNKPLIQGESRFDQTQLNIEKDNWNWSEPSRYDGERPANWYEASLSRHENSAPLAHDAVCDVLVIGSGLLGASAALHL
AEAGVDTILVDKHHVGSAASGRNGGQLTPGLARWEAADMIDYLSHDDAKRLWRFASTESMDLIDEIGARYALDLDRKRGH
VTAAVHPGHMSALLDGADARRHLGDAGVTLVGRHQLHDEYVRSGLYHGAAIDALGGQLHPLALVRGLVHGFQLNGGALYE
GTEVLELDETPAGVVATTPGGTITARRGVVLALHNTTFRLLDDGGATTVPFFTYVSVTAPLDVDVATLMPAGMPVYDTQF
QIDYYRPVRGNRLLFGGQGTGSCWPQQDVNAYLLTRLNTVFPQHDGRFALDYCWSGVSDFTLNGATDSRKTDGRVPMYMV
QGWSGHGVAQTVRIGKAICDDFVGRNDDFSMLTGIDHREIPLGRQLSPIAIPAAKAAMSVMSALNPGRMISF

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=411, Percent_Identity=31.8734793187348, Blast_Score=182, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 51245; Mature: 51114

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTYNKPLIQGESRFDQTQLNIEKDNWNWSEPSRYDGERPANWYEASLSRHENSAPLAHD
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHH
AVCDVLVIGSGLLGASAALHLAEAGVDTILVDKHHVGSAASGRNGGQLTPGLARWEAADM
HHHHHHEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
IDYLSHDDAKRLWRFASTESMDLIDEIGARYALDLDRKRGHVTAAVHPGHMSALLDGADA
HHHHCCCCHHHHHHHCCCCCHHHHHHHCCEEEEEECCCCCCEEEEECCCHHHHHHCCCHH
RRHLGDAGVTLVGRHQLHDEYVRSGLYHGAAIDALGGQLHPLALVRGLVHGFQLNGGALY
HHHCCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHCEEECCCEEE
EGTEVLELDETPAGVVATTPGGTITARRGVVLALHNTTFRLLDDGGATTVPFFTYVSVTA
CCCEEEEECCCCCCEEEECCCCEEEECCCEEEEEECCEEEEEECCCCCEEEEEEEEEEEC
PLDVDVATLMPAGMPVYDTQFQIDYYRPVRGNRLLFGGQGTGSCWPQQDVNAYLLTRLNT
CCCCCHHHHCCCCCCEECCEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHH
VFPQHDGRFALDYCWSGVSDFTLNGATDSRKTDGRVPMYMVQGWSGHGVAQTVRIGKAIC
HCCCCCCCEEEEHHHCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
DDFVGRNDDFSMLTGIDHREIPLGRQLSPIAIPAAKAAMSVMSALNPGRMISF
HHHCCCCCCCHHEECCCCCCCCCCCCCCCEECHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTYNKPLIQGESRFDQTQLNIEKDNWNWSEPSRYDGERPANWYEASLSRHENSAPLAHD
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHH
AVCDVLVIGSGLLGASAALHLAEAGVDTILVDKHHVGSAASGRNGGQLTPGLARWEAADM
HHHHHHEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
IDYLSHDDAKRLWRFASTESMDLIDEIGARYALDLDRKRGHVTAAVHPGHMSALLDGADA
HHHHCCCCHHHHHHHCCCCCHHHHHHHCCEEEEEECCCCCCEEEEECCCHHHHHHCCCHH
RRHLGDAGVTLVGRHQLHDEYVRSGLYHGAAIDALGGQLHPLALVRGLVHGFQLNGGALY
HHHCCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHCEEECCCEEE
EGTEVLELDETPAGVVATTPGGTITARRGVVLALHNTTFRLLDDGGATTVPFFTYVSVTA
CCCEEEEECCCCCCEEEECCCCEEEECCCEEEEEECCEEEEEECCCCCEEEEEEEEEEEC
PLDVDVATLMPAGMPVYDTQFQIDYYRPVRGNRLLFGGQGTGSCWPQQDVNAYLLTRLNT
CCCCCHHHHCCCCCCEECCEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHH
VFPQHDGRFALDYCWSGVSDFTLNGATDSRKTDGRVPMYMVQGWSGHGVAQTVRIGKAIC
HCCCCCCCEEEEHHHCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
DDFVGRNDDFSMLTGIDHREIPLGRQLSPIAIPAAKAAMSVMSALNPGRMISF
HHHCCCCCCCHHEECCCCCCCCCCCCCCCEECHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]