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The map label for this gene is proB

Identifier: 115350541

GI number: 115350541

Start: 550744

End: 551862

Strand: Direct

Name: proB

Synonym: Bamb_0487

Alternate gene names: 115350541

Gene position: 550744-551862 (Clockwise)

Preceding gene: 115350540

Following gene: 115350544

Centisome position: 15.49

GC content: 67.74

Gene sequence:

>1119_bases
ATGCGTTCGATCATCGCGGATTCGAAGCGATTGGTGGTGAAGGTGGGCTCGAGCCTCGTGACCAACGACGGCAAGGGGCT
CGATCATGCTGCAATCGGCCGCTGGGCGGCCCAGATCGCCGCGCTGCGCGCGCAGGGCAAGGAAGTGGTGCTCGTCAGTT
CGGGTGCGATTGCCGAAGGGATGCAGCGGCTCGGCTGGAGCAAGCGTCCGCGCGAGATCGACGAGCTGCAGGCTGCCGCC
GCGGTCGGCCAGATGGGGCTCGCGCAAGTCTATGAAAGCCGCTTTACCGAGCACGACATCCGCACCGCGCAGATCCTGCT
CACGCACGCCGACCTCGCAGATCGCGAACGCTACCTGAATGCACGCTCGACGCTGCTCACGCTGCTGCGGCTCGGCGTCG
TGCCGATCATCAACGAGAACGACACGGTCGTCACCGACGAAATCAAGTTCGGCGACAACGACACGCTCGGCGCACTCGTC
GCGAACCTGATCGAAGGCGACGCACTGATCATCCTGACCGACCAGTCGGGGCTGTTCACCGCCGATCCGCGCAAGGATCC
GAACGCGACGCTCGTCGCGGAAGCCAGCGCGGGCGCGCCGGACCTCGAGGCGATGGCGGGTGGCGCAGGCTCGAGCCTCG
GCCGCGGCGGGATGCTGACGAAGATCCTCGCGGCGAAGCGCGCGGCGCACAGCGGCGCGAACACCGTGATTGCGAGCGGC
CGCGAAACCGACGTGCTGGTGCGGCTCGCGGCCGGTGAGGCGATCGGCACGCAGCTGATCGCGCGCACCGCGCGGATGGC
CGCGCGCAAGCAATGGATGGCCGATCACCTGCAGGTGCGCGGGCACGTCGTGATCGATGCGGGCGCGGTCGAGAAGCTGA
CGGCCGGCGGCAAGAGCCTGCTGCCGATCGGCGTGATCGGCGTGCAGGGTGCGTTCGCGCGCGGCGAGGTCATTGCCTGC
GTTGGCCCCGACGGGCGTGAAGTGGCGCGCGGCCTCACGAACTACAGCAGCGCGGAAACGAAGCTGATTCATCGCAAGCC
GAGCGGCGAGATCGAGACTGTGCTCGGCTACATGCTGGAGCCGGAGCTGATTCACCGCGACAACCTCGTGCTGGTGTAA

Upstream 100 bases:

>100_bases
CGACGCCTGAGCGGACCCGAAACGCACCGGGCATCGCGCCCGGTGCCGGAATTCAATCGCGCCCGGCCGGGCAGGCATCA
GATACAGGAGACGGCGCACG

Downstream 100 bases:

>100_bases
CGGTTCGGCCCGGCGTCGCGAGACAGCCAAAAAGCTAAAAAGCCCGCATTTGCGCGGGCTTTTTCGTTGCGACGAAGCGG
AGCAGGACCGGCCAGGCGGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEGMQRLGWSKRPREIDELQAAA
AVGQMGLAQVYESRFTEHDIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLIEGDALIILTDQSGLFTADPRKDPNATLVAEASAGAPDLEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
RETDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSLLPIGVIGVQGAFARGEVIAC
VGPDGREVARGLTNYSSAETKLIHRKPSGEIETVLGYMLEPELIHRDNLVLV

Sequences:

>Translated_372_residues
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEGMQRLGWSKRPREIDELQAAA
AVGQMGLAQVYESRFTEHDIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLIEGDALIILTDQSGLFTADPRKDPNATLVAEASAGAPDLEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
RETDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSLLPIGVIGVQGAFARGEVIAC
VGPDGREVARGLTNYSSAETKLIHRKPSGEIETVLGYMLEPELIHRDNLVLV
>Mature_372_residues
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEGMQRLGWSKRPREIDELQAAA
AVGQMGLAQVYESRFTEHDIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLIEGDALIILTDQSGLFTADPRKDPNATLVAEASAGAPDLEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
RETDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSLLPIGVIGVQGAFARGEVIAC
VGPDGREVARGLTNYSSAETKLIHRKPSGEIETVLGYMLEPELIHRDNLVLV

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=292, Percent_Identity=37.3287671232877, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI21361368, Length=294, Percent_Identity=37.0748299319728, Blast_Score=145, Evalue=6e-35,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=43.1693989071038, Blast_Score=277, Evalue=8e-76,
Organism=Caenorhabditis elegans, GI17569969, Length=281, Percent_Identity=34.8754448398576, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17569967, Length=281, Percent_Identity=34.8754448398576, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320506, Length=420, Percent_Identity=37.1428571428571, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6321822, Length=414, Percent_Identity=32.6086956521739, Blast_Score=192, Evalue=9e-50,
Organism=Drosophila melanogaster, GI21357643, Length=350, Percent_Identity=31.7142857142857, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_BURA4 (B1YSU8)

Other databases:

- EMBL:   CP001025
- RefSeq:   YP_001807225.1
- ProteinModelPortal:   B1YSU8
- GeneID:   6176526
- GenomeReviews:   CP001025_GR
- KEGG:   bac:BamMC406_0512
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   BAMB398577:BAMMC406_0512-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39216; Mature: 39216

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEG
CCCCCCCCCEEEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHH
MQRLGWSKRPREIDELQAAAAVGQMGLAQVYESRFTEHDIRTAQILLTHADLADRERYLN
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHH
ARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALVANLIEGDALIILTDQSGLFT
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEE
ADPRKDPNATLVAEASAGAPDLEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
CCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECC
RETDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSL
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCC
LPIGVIGVQGAFARGEVIACVGPDGREVARGLTNYSSAETKLIHRKPSGEIETVLGYMLE
CEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHEEEECCCCCCHHHHHHHHCC
PELIHRDNLVLV
CHHCCCCCEEEC
>Mature Secondary Structure
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEG
CCCCCCCCCEEEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHH
MQRLGWSKRPREIDELQAAAAVGQMGLAQVYESRFTEHDIRTAQILLTHADLADRERYLN
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHH
ARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALVANLIEGDALIILTDQSGLFT
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEE
ADPRKDPNATLVAEASAGAPDLEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
CCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECC
RETDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSL
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCC
LPIGVIGVQGAFARGEVIACVGPDGREVARGLTNYSSAETKLIHRKPSGEIETVLGYMLE
CEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHEEEECCCCCCHHHHHHHHCC
PELIHRDNLVLV
CHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA