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The map label for this gene is proS [H]

Identifier: 115350544

GI number: 115350544

Start: 553467

End: 555203

Strand: Direct

Name: proS [H]

Synonym: Bamb_0490

Alternate gene names: 115350544

Gene position: 553467-555203 (Clockwise)

Preceding gene: 115350541

Following gene: 115350545

Centisome position: 15.56

GC content: 65.69

Gene sequence:

>1737_bases
ATGAAAGCTTCCCGTTTCTTTATCGGCACCCTCAAAGAAGCACCGGCCGACGCAGAGATCGTCAGCCACAAGCTGATGGT
GCGCGCCGGCATGATCCGTCGTGTCGCCGGCGGCATCTATAACTACCTGCCGGTCGGCCTGCGTTCGATTCGCAAGGTCG
AAGCGATCGTGCGCGAGGAAATGAACCGGGCAGGCGCCATCGAGCTGCTGATGCCGGCCGTGCAGCCGGCCGAGCTGTGG
CAGGAGTCGGGCCGCTGGGAACAGTACGGCCCCGAGCTGCTGCGCTTCAAGGACCGCAAGGACAACGACTTCGTGATCGG
GCCGACGCACGAGGAAGTGGTCACCGACATCGCGCGCAACCAGATCAAGAGCTACCGGCAGATGCCGGTGAACTTCTACC
AGATCCAGACGAAGTTCCGCGACGAGATCCGTCCGCGCTTCGGCGTGATGCGCGGCCGCGAATTCCTCATGAAGGACGCG
TATTCGTTCGACAAGGACACGGCCGGCCTGAACGAGTCGTATCGCAAGATGTACGACGCGTACGTGCGCATCTTCACGCG
CCTCGGCCTCGAGTTCCGGGCGGTCGCGGCCGACAGCGGCTCGATCGGCGGCAACTTCTCGCACGAGTTCCACGTGATCG
CTGATACCGGCGAGGACGCGATCGCGTACTGCCCGACGTCCGACTTCGCGGCGAACATCGAAGCGGCCGAGGCGCTGCCG
TTGATCGCCGAGCGTGCCGCACCGGCCGAAGCGATGGAGAAGGTCGCGACGCCCGGCAAGGCGAAGTGCGAAGCGGTCGC
CGAACTGCTGGCAATCCCGCTCGAGCGCACGATCAAGTCGATCGTGCTCGCCACCGAGAACGAAGGCGCCGAGCCGACCA
TCTGGCTCATCATGCTGCGCGGCGACCACGACCTGAACGAGATCAAGGTGTCGAAGCTGCCGGGGCTGAAGAACCACCGC
TTCGCGACCGAGCAGGAAATCGTCGACTGGTTCGGCACGCCGCCGGGCTATCTCGGCCCGGTCGGCACGAAGAAGCCGGT
GAAGGTGATCGCCGATCGCACGGTCGCGAACATGAGCGACTTCGTCGTCGGCGCGAACGAGGTCGACTATCACATCGCGG
GCGTGAACTGGGGCCGCGACCTGCCGGAACCGGAAGTCGCCGATGTGCGCGACGTGAAGAAGGGCGATCCGTCGCCGGAC
GGCAAGGGCACGATCGACATCTGCCGCGGCATCGAAGTCGGTCACGTGTTCCAGCTCGGCACCAAGTATTCGGACGCGAT
GGGCGCGACGTTCCTCGATGAATCGGGCAAGCCGCAGCCGATGCTGATGGGCTGCTACGGCGTCGGCATCACGCGGATTC
TCGGCGCGGCGATCGAACAGAACTTCGACGATAAGGGCATCATCTGGCCCGAGTCGATCGCGCCGTTCGAGGTCGTGCTG
TGCCCGATGGGCTACGATCGCAGCGACATGGTCCGCGAGGCCGCCGACAAGCTGTACGCGGAACTGGCCGCCGCCGGCAT
CGACGTGATCCTCGACGATCGCGGCGAGCGCCCGGGCGTGATGTTCGCCGACTGGGAACTGATCGGCGTGCCGCATCGCC
TGGTGATCGGCGAGCGCGGCCTGAAGGAAGGCAAGATCGAGTACCAGGGCCGCCGCGACGCGGAGGCGACGCTGCTGCCG
GCGGACGAGGCTGCGGCGACGGTGACGGAGAAGATCCGCGCCGCGCTCGCGCACTAA

Upstream 100 bases:

>100_bases
CTACGTCATCTGCGCCATCGTCGTTCGTCGCGCGCAGCTTTTTCACCTTGAAACTTTTTCGGGCGCCCCGCCTGGGGGCG
CCGTATTTGGAACCGTCCGC

Downstream 100 bases:

>100_bases
GCGCCGCGACCGGGGGACACGGTGGAATACACCTTCCTGTCGGCCACCGTGCTCCTCGTGCTGATTACGGATCCACTCGG
CAACATCCCGCTGTTCATCA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPVGLRSIRKVEAIVREEMNRAGAIELLMPAVQPAELW
QESGRWEQYGPELLRFKDRKDNDFVIGPTHEEVVTDIARNQIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFLMKDA
YSFDKDTAGLNESYRKMYDAYVRIFTRLGLEFRAVAADSGSIGGNFSHEFHVIADTGEDAIAYCPTSDFAANIEAAEALP
LIAERAAPAEAMEKVATPGKAKCEAVAELLAIPLERTIKSIVLATENEGAEPTIWLIMLRGDHDLNEIKVSKLPGLKNHR
FATEQEIVDWFGTPPGYLGPVGTKKPVKVIADRTVANMSDFVVGANEVDYHIAGVNWGRDLPEPEVADVRDVKKGDPSPD
GKGTIDICRGIEVGHVFQLGTKYSDAMGATFLDESGKPQPMLMGCYGVGITRILGAAIEQNFDDKGIIWPESIAPFEVVL
CPMGYDRSDMVREAADKLYAELAAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERGLKEGKIEYQGRRDAEATLLP
ADEAAATVTEKIRAALAH

Sequences:

>Translated_578_residues
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPVGLRSIRKVEAIVREEMNRAGAIELLMPAVQPAELW
QESGRWEQYGPELLRFKDRKDNDFVIGPTHEEVVTDIARNQIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFLMKDA
YSFDKDTAGLNESYRKMYDAYVRIFTRLGLEFRAVAADSGSIGGNFSHEFHVIADTGEDAIAYCPTSDFAANIEAAEALP
LIAERAAPAEAMEKVATPGKAKCEAVAELLAIPLERTIKSIVLATENEGAEPTIWLIMLRGDHDLNEIKVSKLPGLKNHR
FATEQEIVDWFGTPPGYLGPVGTKKPVKVIADRTVANMSDFVVGANEVDYHIAGVNWGRDLPEPEVADVRDVKKGDPSPD
GKGTIDICRGIEVGHVFQLGTKYSDAMGATFLDESGKPQPMLMGCYGVGITRILGAAIEQNFDDKGIIWPESIAPFEVVL
CPMGYDRSDMVREAADKLYAELAAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERGLKEGKIEYQGRRDAEATLLP
ADEAAATVTEKIRAALAH
>Mature_578_residues
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPVGLRSIRKVEAIVREEMNRAGAIELLMPAVQPAELW
QESGRWEQYGPELLRFKDRKDNDFVIGPTHEEVVTDIARNQIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFLMKDA
YSFDKDTAGLNESYRKMYDAYVRIFTRLGLEFRAVAADSGSIGGNFSHEFHVIADTGEDAIAYCPTSDFAANIEAAEALP
LIAERAAPAEAMEKVATPGKAKCEAVAELLAIPLERTIKSIVLATENEGAEPTIWLIMLRGDHDLNEIKVSKLPGLKNHR
FATEQEIVDWFGTPPGYLGPVGTKKPVKVIADRTVANMSDFVVGANEVDYHIAGVNWGRDLPEPEVADVRDVKKGDPSPD
GKGTIDICRGIEVGHVFQLGTKYSDAMGATFLDESGKPQPMLMGCYGVGITRILGAAIEQNFDDKGIIWPESIAPFEVVL
CPMGYDRSDMVREAADKLYAELAAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERGLKEGKIEYQGRRDAEATLLP
ADEAAATVTEKIRAALAH

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=241, Percent_Identity=42.3236514522822, Blast_Score=222, Evalue=8e-58,
Organism=Escherichia coli, GI1786392, Length=574, Percent_Identity=56.2717770034843, Blast_Score=684, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=252, Percent_Identity=38.4920634920635, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI193203271, Length=82, Percent_Identity=41.4634146341463, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=221, Percent_Identity=41.1764705882353, Blast_Score=192, Evalue=8e-50,
Organism=Drosophila melanogaster, GI24656200, Length=245, Percent_Identity=37.9591836734694, Blast_Score=180, Evalue=3e-45,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63903; Mature: 63903

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPVGLRSIRKVEAIVREE
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAIELLMPAVQPAELWQESGRWEQYGPELLRFKDRKDNDFVIGPTHEEVVTDIARN
HHHCCCHHHHHCCCCHHHHHHHCCCHHHCCCHHHHCCCCCCCCEEECCCHHHHHHHHHHH
QIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFLMKDAYSFDKDTAGLNESYRKMYDA
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
YVRIFTRLGLEFRAVAADSGSIGGNFSHEFHVIADTGEDAIAYCPTSDFAANIEAAEALP
HHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEECCCCCHHCCCHHHHHHH
LIAERAAPAEAMEKVATPGKAKCEAVAELLAIPLERTIKSIVLATENEGAEPTIWLIMLR
HHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEEEE
GDHDLNEIKVSKLPGLKNHRFATEQEIVDWFGTPPGYLGPVGTKKPVKVIADRTVANMSD
CCCCCCCEEHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHH
FVVGANEVDYHIAGVNWGRDLPEPEVADVRDVKKGDPSPDGKGTIDICRGIEVGHVFQLG
HEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCEEECC
TKYSDAMGATFLDESGKPQPMLMGCYGVGITRILGAAIEQNFDDKGIIWPESIAPFEVVL
CCHHHHHCCCEECCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
CPMGYDRSDMVREAADKLYAELAAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERG
ECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECEEEECCCCEEEECCCC
LKEGKIEYQGRRDAEATLLPADEAAATVTEKIRAALAH
CCCCCEEECCCCCCCEEEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRVAGGIYNYLPVGLRSIRKVEAIVREE
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAIELLMPAVQPAELWQESGRWEQYGPELLRFKDRKDNDFVIGPTHEEVVTDIARN
HHHCCCHHHHHCCCCHHHHHHHCCCHHHCCCHHHHCCCCCCCCEEECCCHHHHHHHHHHH
QIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFLMKDAYSFDKDTAGLNESYRKMYDA
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
YVRIFTRLGLEFRAVAADSGSIGGNFSHEFHVIADTGEDAIAYCPTSDFAANIEAAEALP
HHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEECCCCCHHCCCHHHHHHH
LIAERAAPAEAMEKVATPGKAKCEAVAELLAIPLERTIKSIVLATENEGAEPTIWLIMLR
HHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEEEE
GDHDLNEIKVSKLPGLKNHRFATEQEIVDWFGTPPGYLGPVGTKKPVKVIADRTVANMSD
CCCCCCCEEHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHH
FVVGANEVDYHIAGVNWGRDLPEPEVADVRDVKKGDPSPDGKGTIDICRGIEVGHVFQLG
HEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCEEECC
TKYSDAMGATFLDESGKPQPMLMGCYGVGITRILGAAIEQNFDDKGIIWPESIAPFEVVL
CCHHHHHCCCEECCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
CPMGYDRSDMVREAADKLYAELAAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERG
ECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECEEEECCCCEEEECCCC
LKEGKIEYQGRRDAEATLLPADEAAATVTEKIRAALAH
CCCCCEEECCCCCCCEEEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA