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The map label for this gene is murD

Identifier: 115350515

GI number: 115350515

Start: 522527

End: 524035

Strand: Direct

Name: murD

Synonym: Bamb_0461

Alternate gene names: 115350515

Gene position: 522527-524035 (Clockwise)

Preceding gene: 115350514

Following gene: 115350516

Centisome position: 14.69

GC content: 71.37

Gene sequence:

>1509_bases
ATGTTTGGAGATCGGCAGCGGCCGATGGTGCTCGTACTGGGGCTCGGGGAATCGGGCCTCGCGATCGCGCGATGGTGCGC
GAGGCACGGGTGCCGGCTGCGTATTGCCGATACCCGCGAGGCGCCGCCTAACCTTGCCGCGCTGCAGGCCGAAGGCATCG
ATGCCGAGTTCGTCGGCGGGCCGTTCACGCCCGCGCTGCTCGACGGCGGTGTCGAGATCGTCGGCCTGAGCCCTGGCCTG
TCGCCGCTCGAACCGGCACTCGCCGCGCTGATCGCGGCCGCGAACGAGCGCGCGATCGCGGTGTGGGGCGAGCTCGAATT
CTTCGCGCAGGCGCTGCGCGCGCTCGGCACGAGCGGCTACCAGCCGAAGGTGCTCGCGATCACCGGCACCAACGGCAAGA
CCACGACGACGAACCTCACGGGCCTGCTGTGCCAGCGTTCGGGCAAGAAGGTCGCGGTCGCCGGCAACATCAGCCCGGCG
ATGCTCGACCGGCTCGCGCGCGCGATCGACGAGACGGCGCTGCCCGACGTCTGGGTGCTCGAACTGTCGAGCTTCCAGCT
CGAGACGGCGCGCACGTTCGCGCCCGACGCGGCCGCGATTCTCAACATCACGCAGGATCACCTCGACTGGCATGGCAGCT
TCGACGCGTATGCGGCGGCGAAGGGCCGGATCTTCGGCGCGACGACCACCCGCGTGCTCAATCGCGACGATGCGGCCGTG
ATGAAGTTCGCGCCGGCCGCCGGCGCGGCCGATGCGGCGCGCACGGTCACGTTCGGCCTGAACGAACCGGCGCAGCAGGG
CGACTACGGCCTGTCGCGCGACAACGGCATCGCGTGGCTGGTCGAGGCGGTCGACCGCGACGCGCCGGACGAGACGACGA
GCCGCCGGCGCAAGCGCGACGGCGCGCATACGCCCGACATTGCACAAAAGCGCCTGATGCCGGCCGACGCGCTGCGCATC
CGCGGGCTGCACAACGCGGCGAACGCGCTGGCCGCGTTCGCGCTCGCGCGTGCGATCGACCTGCCGGCCGCGCCGCTGCT
GCACGCGCTGCGCGAATACCGCGGCGAAGCGCATCGCGTCGAAGTGATCGCGACGATCGACGACGTGGACTACGTCGACG
ACAGCAAGGGAACCAACGTCGGCGCGACGGTGGCCGCGCTCGACGGACTCGCGCAGAAGATCGTGCTGATCGCGGGCGGT
GACGGCAAGGGGCAGGATTTCGCGCCGCTCGTCGCGCCCGTCGCGCGGTGGTGCCGCGCGGTGATGCTGATCGGCCGCGA
TGCGCCGGCGATCCGCGACACGCTCGCCGAGACGGGCGTGCCGCTTGCCGACCACGCGACGCTCGAGGCGGCGGTGCATG
CGGCGGCCGAGCTCGCGGAGCCGGGCGACGCGGTGCTGCTGTCGCCCGCTTGCGCGAGCCTCGACATGTTCAGGAACTAC
GCCCACCGCGCTGACGTGTTCCGCGCGGCCGTGGACGAAATCGCCATCGACAAAGGAGCGACGACATGA

Upstream 100 bases:

>100_bases
GGTGGTGGTGCGTTTCTGGATCATCACGCTGATGCTGTGCCTGTTCGGTCTGTCCACCCTCAAGTTGCGGTAAAGGAAAG
GTAAAAGGATGTCTGGCGAG

Downstream 100 bases:

>100_bases
GCTGGTCCGATCGCCTCGTCTCCCGTTTCAACGACCGGCGCGGCACCGGCGGCAATGCCGCGGGCGGCCGCGTCGCGTCG
GCCACGCGCGCCGCGACGGG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALQAEGIDAEFVGGPFTPALLDGGVEIVGLSPGL
SPLEPALAALIAAANERAIAVWGELEFFAQALRALGTSGYQPKVLAITGTNGKTTTTNLTGLLCQRSGKKVAVAGNISPA
MLDRLARAIDETALPDVWVLELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAAAKGRIFGATTTRVLNRDDAAV
MKFAPAAGAADAARTVTFGLNEPAQQGDYGLSRDNGIAWLVEAVDRDAPDETTSRRRKRDGAHTPDIAQKRLMPADALRI
RGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHRVEVIATIDDVDYVDDSKGTNVGATVAALDGLAQKIVLIAGG
DGKGQDFAPLVAPVARWCRAVMLIGRDAPAIRDTLAETGVPLADHATLEAAVHAAAELAEPGDAVLLSPACASLDMFRNY
AHRADVFRAAVDEIAIDKGATT

Sequences:

>Translated_502_residues
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALQAEGIDAEFVGGPFTPALLDGGVEIVGLSPGL
SPLEPALAALIAAANERAIAVWGELEFFAQALRALGTSGYQPKVLAITGTNGKTTTTNLTGLLCQRSGKKVAVAGNISPA
MLDRLARAIDETALPDVWVLELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAAAKGRIFGATTTRVLNRDDAAV
MKFAPAAGAADAARTVTFGLNEPAQQGDYGLSRDNGIAWLVEAVDRDAPDETTSRRRKRDGAHTPDIAQKRLMPADALRI
RGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHRVEVIATIDDVDYVDDSKGTNVGATVAALDGLAQKIVLIAGG
DGKGQDFAPLVAPVARWCRAVMLIGRDAPAIRDTLAETGVPLADHATLEAAVHAAAELAEPGDAVLLSPACASLDMFRNY
AHRADVFRAAVDEIAIDKGATT
>Mature_502_residues
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALQAEGIDAEFVGGPFTPALLDGGVEIVGLSPGL
SPLEPALAALIAAANERAIAVWGELEFFAQALRALGTSGYQPKVLAITGTNGKTTTTNLTGLLCQRSGKKVAVAGNISPA
MLDRLARAIDETALPDVWVLELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAAAKGRIFGATTTRVLNRDDAAV
MKFAPAAGAADAARTVTFGLNEPAQQGDYGLSRDNGIAWLVEAVDRDAPDETTSRRRKRDGAHTPDIAQKRLMPADALRI
RGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHRVEVIATIDDVDYVDDSKGTNVGATVAALDGLAQKIVLIAGG
DGKGQDFAPLVAPVARWCRAVMLIGRDAPAIRDTLAETGVPLADHATLEAAVHAAAELAEPGDAVLLSPACASLDMFRNY
AHRADVFRAAVDEIAIDKGATT

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=502, Percent_Identity=35.8565737051793, Blast_Score=219, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 52794; Mature: 52794

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALQAEGIDAEFVGG
CCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCEECCC
PFTPALLDGGVEIVGLSPGLSPLEPALAALIAAANERAIAVWGELEFFAQALRALGTSGY
CCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCC
QPKVLAITGTNGKTTTTNLTGLLCQRSGKKVAVAGNISPAMLDRLARAIDETALPDVWVL
CCEEEEEECCCCCEEECHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEE
ELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAAAKGRIFGATTTRVLNRDDAAV
EECCCEEHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCEEEECHHHHHHCCCCCHH
MKFAPAAGAADAARTVTFGLNEPAQQGDYGLSRDNGIAWLVEAVDRDAPDETTSRRRKRD
EEECCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCCCEEEEEHHCCCCCCHHHHHHHHHC
GAHTPDIAQKRLMPADALRIRGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHRV
CCCCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEE
EVIATIDDVDYVDDSKGTNVGATVAALDGLAQKIVLIAGGDGKGQDFAPLVAPVARWCRA
EEEEEECCCCCCCCCCCCCCCHHHHHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
VMLIGRDAPAIRDTLAETGVPLADHATLEAAVHAAAELAEPGDAVLLSPACASLDMFRNY
HHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHH
AHRADVFRAAVDEIAIDKGATT
HHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALQAEGIDAEFVGG
CCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCEECCC
PFTPALLDGGVEIVGLSPGLSPLEPALAALIAAANERAIAVWGELEFFAQALRALGTSGY
CCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCC
QPKVLAITGTNGKTTTTNLTGLLCQRSGKKVAVAGNISPAMLDRLARAIDETALPDVWVL
CCEEEEEECCCCCEEECHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEE
ELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAAAKGRIFGATTTRVLNRDDAAV
EECCCEEHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCEEEECHHHHHHCCCCCHH
MKFAPAAGAADAARTVTFGLNEPAQQGDYGLSRDNGIAWLVEAVDRDAPDETTSRRRKRD
EEECCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCCCEEEEEHHCCCCCCHHHHHHHHHC
GAHTPDIAQKRLMPADALRIRGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHRV
CCCCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEE
EVIATIDDVDYVDDSKGTNVGATVAALDGLAQKIVLIAGGDGKGQDFAPLVAPVARWCRA
EEEEEECCCCCCCCCCCCCCCHHHHHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
VMLIGRDAPAIRDTLAETGVPLADHATLEAAVHAAAELAEPGDAVLLSPACASLDMFRNY
HHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHH
AHRADVFRAAVDEIAIDKGATT
HHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA