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The map label for this gene is murE

Identifier: 115350512

GI number: 115350512

Start: 518372

End: 519910

Strand: Direct

Name: murE

Synonym: Bamb_0458

Alternate gene names: 115350512

Gene position: 518372-519910 (Clockwise)

Preceding gene: 115350511

Following gene: 115350513

Centisome position: 14.58

GC content: 70.63

Gene sequence:

>1539_bases
ATGAGCGCCGCTCGCAGTTCCCATCCGGCGCATCAGCAGATCGCAGCCGCGCTTGCGTGGCTGCGCCAGCATGTGGCCCC
CGCGGCGCAACTGCATGCCGACACGCGCAGCCTGCGGGCCGGCGACGTGTTTCTCGGCTATGCGGTCGACGGGGCAGACA
ATCGCGCCTTCATTGCCGATGCCGTCGCGCGTGGCGCGGCCGCCGTGCTGTATCAGCCGGAAGGCCTCGCGGCCGCACCG
GCCGTGCCCGTCGCACTCGCGGTGCCGGCGCTCGACCAACTGGCCGGCGAGATCGCCAGCGGCTGGTACGGCGACCCGAG
CGACAGCATGCTCGCAATCGGCGTGACCGGCACCAACGGCAAGACGTCGTGCACGCAATGGATCGCCGCCGCGCTGACGG
CGCTGCACCAGCCGTGCGCGGTGATCGGCACGCTCGGCAGCGGCATGCACGGGCATCTGGTGCCGACCGGCTTCACGACG
CCGGACGCGCCGCAGCTGCAGCGCAGCCTCGCGCAGTTGCACGACGCGGGCGCGAAGGCCGTCGCGATGGAAGTGTCGTC
GCACGCGTTGCATCAGGGCCGCGTGAACGGCACGGCATTCGACATCGCCGTGTTCACGAACCTCACCCAGGATCACCTCG
ACTATCACGGCACGTTCGACGCGTACGAAGCCGCGAAGGCGAAGCTGTTCGCGTGGCGCGGCCTGCGCGCGGCGGTCGTC
AATCGCGACGACGCAGCCGGCCGCCGCCTGCTCGAGAAGCTGGCCGGCCGCGTGCGCACGATCGCATACGGAATCGGCGA
CGCGCAGGCGCCCGACGCCGATCGCGAGCTGGTCGCGCTCGACGTGCGCGCCACCGCCACGGGCACGGCATTCCACCTGC
GTTCGTCGTGGGGCGACGCTGACGTCGAGGTCGGTACGCTCGGCGCGTTCAACGTCAGCAATCTGCTCGCGGTGCTCGGC
TCGCTGCTCGCGGCCGACGTGCCGTTCGACGCGGCGGTCGCGGAGATCGCACGGCTCGAGTCCGTCAATGGCCGCATGCA
GCGCCTGGGCGGCCGGCTGCAGAACGACGAACCGCTGGTCGTGATCGACTATGCGCATACGCCCGATGCGCTCGAGAAGA
CGCTCGACGCGTTGCGCCCGATCGCGGCCGCACGCGGCGGTCGGCTCGTGTGCATGTTCGGCTGCGGCGGCGATCGCGAT
GCGACGAAGCGCCCGCTGATGGGCGTGATCGCCGAGCGGCTCGCCGATGAAGTCGTCGTCACGAGCGACAACCCGCGCAG
CGAAGATCCGCAGCGAATCATCGACCAGGTCATCGCGGGCATGACGGCGCCCGATCACGCGCGCCGCATCGAGGATCGCG
CAAGCGCGATCCTGCAGGCCGTGCGTGGCGCCGCCCGCGAGGATGTCGTCGTGCTGGCCGGCAAGGGTCACGAGGCGACG
CAGGAAATCATGGGCAAGAAGCGCGCGTTCTCCGATCAGGATCACGCACGGCTCGCGCTCGCGGCACGCGCGACGCACGT
CAAGGGAGGCGCTGAATGA

Upstream 100 bases:

>100_bases
GCGCGCCCGCCAAGCCGGGTCCGCAAAAGCTCGCCGCGGGCAGCGGTGCGAAGCATATGATCGTATCCAGCACGACACGT
AATTCACCAGGAGTTGTTCG

Downstream 100 bases:

>100_bases
CGATGCTCAGTCTCGGCGAAGCCGCCCGCCTGATTCCTGGCGCCACCGTCCACGGCGATCCGGACACGACGTTCGAGCGC
GTGTCGACGGACAGCCGCAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MSAARSSHPAHQQIAAALAWLRQHVAPAAQLHADTRSLRAGDVFLGYAVDGADNRAFIADAVARGAAAVLYQPEGLAAAP
AVPVALAVPALDQLAGEIASGWYGDPSDSMLAIGVTGTNGKTSCTQWIAAALTALHQPCAVIGTLGSGMHGHLVPTGFTT
PDAPQLQRSLAQLHDAGAKAVAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHGTFDAYEAAKAKLFAWRGLRAAVV
NRDDAAGRRLLEKLAGRVRTIAYGIGDAQAPDADRELVALDVRATATGTAFHLRSSWGDADVEVGTLGAFNVSNLLAVLG
SLLAADVPFDAAVAEIARLESVNGRMQRLGGRLQNDEPLVVIDYAHTPDALEKTLDALRPIAAARGGRLVCMFGCGGDRD
ATKRPLMGVIAERLADEVVVTSDNPRSEDPQRIIDQVIAGMTAPDHARRIEDRASAILQAVRGAAREDVVVLAGKGHEAT
QEIMGKKRAFSDQDHARLALAARATHVKGGAE

Sequences:

>Translated_512_residues
MSAARSSHPAHQQIAAALAWLRQHVAPAAQLHADTRSLRAGDVFLGYAVDGADNRAFIADAVARGAAAVLYQPEGLAAAP
AVPVALAVPALDQLAGEIASGWYGDPSDSMLAIGVTGTNGKTSCTQWIAAALTALHQPCAVIGTLGSGMHGHLVPTGFTT
PDAPQLQRSLAQLHDAGAKAVAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHGTFDAYEAAKAKLFAWRGLRAAVV
NRDDAAGRRLLEKLAGRVRTIAYGIGDAQAPDADRELVALDVRATATGTAFHLRSSWGDADVEVGTLGAFNVSNLLAVLG
SLLAADVPFDAAVAEIARLESVNGRMQRLGGRLQNDEPLVVIDYAHTPDALEKTLDALRPIAAARGGRLVCMFGCGGDRD
ATKRPLMGVIAERLADEVVVTSDNPRSEDPQRIIDQVIAGMTAPDHARRIEDRASAILQAVRGAAREDVVVLAGKGHEAT
QEIMGKKRAFSDQDHARLALAARATHVKGGAE
>Mature_511_residues
SAARSSHPAHQQIAAALAWLRQHVAPAAQLHADTRSLRAGDVFLGYAVDGADNRAFIADAVARGAAAVLYQPEGLAAAPA
VPVALAVPALDQLAGEIASGWYGDPSDSMLAIGVTGTNGKTSCTQWIAAALTALHQPCAVIGTLGSGMHGHLVPTGFTTP
DAPQLQRSLAQLHDAGAKAVAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHGTFDAYEAAKAKLFAWRGLRAAVVN
RDDAAGRRLLEKLAGRVRTIAYGIGDAQAPDADRELVALDVRATATGTAFHLRSSWGDADVEVGTLGAFNVSNLLAVLGS
LLAADVPFDAAVAEIARLESVNGRMQRLGGRLQNDEPLVVIDYAHTPDALEKTLDALRPIAAARGGRLVCMFGCGGDRDA
TKRPLMGVIAERLADEVVVTSDNPRSEDPQRIIDQVIAGMTAPDHARRIEDRASAILQAVRGAAREDVVVLAGKGHEATQ
EIMGKKRAFSDQDHARLALAARATHVKGGAE

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=470, Percent_Identity=43.8297872340426, Blast_Score=309, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53688; Mature: 53557

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAARSSHPAHQQIAAALAWLRQHVAPAAQLHADTRSLRAGDVFLGYAVDGADNRAFIAD
CCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHH
AVARGAAAVLYQPEGLAAAPAVPVALAVPALDQLAGEIASGWYGDPSDSMLAIGVTGTNG
HHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
KTSCTQWIAAALTALHQPCAVIGTLGSGMHGHLVPTGFTTPDAPQLQRSLAQLHDAGAKA
CHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCE
VAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHGTFDAYEAAKAKLFAWRGLRAAVV
EEEHHHHHHHHCCCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEE
NRDDAAGRRLLEKLAGRVRTIAYGIGDAQAPDADRELVALDVRATATGTAFHLRSSWGDA
CCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCC
DVEVGTLGAFNVSNLLAVLGSLLAADVPFDAAVAEIARLESVNGRMQRLGGRLQNDEPLV
CEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
VIDYAHTPDALEKTLDALRPIAAARGGRLVCMFGCGGDRDATKRPLMGVIAERLADEVVV
EEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEE
TSDNPRSEDPQRIIDQVIAGMTAPDHARRIEDRASAILQAVRGAAREDVVVLAGKGHEAT
ECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHH
QEIMGKKRAFSDQDHARLALAARATHVKGGAE
HHHHHHHHCCCCCCHHHEEHHHHHHCCCCCCC
>Mature Secondary Structure 
SAARSSHPAHQQIAAALAWLRQHVAPAAQLHADTRSLRAGDVFLGYAVDGADNRAFIAD
CCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHH
AVARGAAAVLYQPEGLAAAPAVPVALAVPALDQLAGEIASGWYGDPSDSMLAIGVTGTNG
HHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
KTSCTQWIAAALTALHQPCAVIGTLGSGMHGHLVPTGFTTPDAPQLQRSLAQLHDAGAKA
CHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCE
VAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHGTFDAYEAAKAKLFAWRGLRAAVV
EEEHHHHHHHHCCCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEE
NRDDAAGRRLLEKLAGRVRTIAYGIGDAQAPDADRELVALDVRATATGTAFHLRSSWGDA
CCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCC
DVEVGTLGAFNVSNLLAVLGSLLAADVPFDAAVAEIARLESVNGRMQRLGGRLQNDEPLV
CEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
VIDYAHTPDALEKTLDALRPIAAARGGRLVCMFGCGGDRDATKRPLMGVIAERLADEVVV
EEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEE
TSDNPRSEDPQRIIDQVIAGMTAPDHARRIEDRASAILQAVRGAAREDVVVLAGKGHEAT
ECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHH
QEIMGKKRAFSDQDHARLALAARATHVKGGAE
HHHHHHHHCCCCCCHHHEEHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]