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The map label for this gene is murF [H]

Identifier: 115350513

GI number: 115350513

Start: 519907

End: 521313

Strand: Direct

Name: murF [H]

Synonym: Bamb_0459

Alternate gene names: 115350513

Gene position: 519907-521313 (Clockwise)

Preceding gene: 115350512

Following gene: 115350514

Centisome position: 14.62

GC content: 71.36

Gene sequence:

>1407_bases
ATGACGATGCTCAGTCTCGGCGAAGCCGCCCGCCTGATTCCTGGCGCCACCGTCCACGGCGATCCGGACACGACGTTCGA
GCGCGTGTCGACGGACAGCCGCACGGTCGGCCCGGGCGACTTGTTCGTCGCGCTGAAGGGCGACCGCTTCGACGCGCACG
ACTTCCTCGGCGACGTGGCCGCGCGTGGCGCAGCCGCGGCGCTCGTCGCGCACGTGCCGGCGGGTCTCGCGATGCCGGTC
GTCGAGGGCGGTGAAACGCGTGCCGCGCTCGGCGCGCTCGCGCACGGCTGGCGCAAGCGCTTCGCGGTGCCGCTCGTCGC
GGTGACGGGCAGTAACGGCAAGACGACCGTCAAGGAAATGATCGCGTCGATCTTCGCGGCGGCAGTCGGCGCCGACGCGC
GACTGGCGACGGCCGGCAACCTGAACAACGACATCGGCGTGCCGCTGACGCTGCTGCGCCTGTCCGGCGCGCATCGCGTC
GCGGTGATCGAACTCGGGATGAACCATCCGGGCGAGACGGAAATCCTCGCGCGCCTCGCGGCACCGACCGTCGCGCTCGT
CAACAACGCGCAGCGCGAGCACCAGGAATTCATGGCCACCGTCGAAGCCGTTGCGCTCGAGCACGCGGCCGTGATCCACG
CGCTGACGCCGGACGGCGTCGCGGTGTTCCCGGCCGACGATGCGTACGCGAGCATCTGGCGCGTCGCGGCGACCGGCAAC
CCGATCCTCGATTTCGCGCTGCACGATGGCGAACGGCAGGTCGAAGCGCAGGTCACGGGCCGTCTGCACGGCGGCGAGCT
TGCGATCGACACGCCGTCGGGCGCGGTAACGGTGCGGCTGCGCGCGCTCGGCGAGCACAACGCACGCAACGCATTGGCTG
CTGCTGCTGCTGCGCTCGCGGCCGGTATCGAACTGGCCGCGATCAAGCAGGGCCTCGAATCGTTCGAGCCGGTCAAGGGG
CGTCTGCAGGTCCGCCAGGCGGACGTCGGCAGCCTCGCGGGCGCGACGGTCGTCGATGACACCTACAACGCGAACCCCGA
CTCGATGCGCGCGGCGATCGACGTGCTGGCCGCGCAACCGGCGCCGCGCGTGTTGGTGATCGGCGACATGGGCGAGGTCG
GCGACGAAGGCCCGGCATTCCACCGCGAGATCGGCGCGTATGCGCGCGAGCGCGGCATCGATGCGCTGTTCGCGCTCGGC
GACGCGTCGCGCGATGCGTGCACGGCGTACGGCGACACGGCCCGCCATTTCGGCGACGTCGGCGCGCTGTTGACTGCGCT
GCTCGCGGCGGGCTACGGCGCGCAGGCCACGGTGCTCGTGAAGGGCTCGCGGTACATGAAGATGGAGCGCGTGGTCGACG
CGCTGACGAACCAATCCGCGGCGGGCACGGTGCCCGCCGCACACTGA

Upstream 100 bases:

>100_bases
CGACGCAGGAAATCATGGGCAAGAAGCGCGCGTTCTCCGATCAGGATCACGCACGGCTCGCGCTCGCGGCACGCGCGACG
CACGTCAAGGGAGGCGCTGA

Downstream 100 bases:

>100_bases
GTAGAAGGAAGGAAGCATGCTGCTGGCGCTGGCGCAATGGCTGCAGGGCGACGCAAGCTTTTTGCGCTTGTTCACGTACC
TGACGTTCCGTGCGGTGATG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTMLSLGEAARLIPGATVHGDPDTTFERVSTDSRTVGPGDLFVALKGDRFDAHDFLGDVAARGAAAALVAHVPAGLAMPV
VEGGETRAALGALAHGWRKRFAVPLVAVTGSNGKTTVKEMIASIFAAAVGADARLATAGNLNNDIGVPLTLLRLSGAHRV
AVIELGMNHPGETEILARLAAPTVALVNNAQREHQEFMATVEAVALEHAAVIHALTPDGVAVFPADDAYASIWRVAATGN
PILDFALHDGERQVEAQVTGRLHGGELAIDTPSGAVTVRLRALGEHNARNALAAAAAALAAGIELAAIKQGLESFEPVKG
RLQVRQADVGSLAGATVVDDTYNANPDSMRAAIDVLAAQPAPRVLVIGDMGEVGDEGPAFHREIGAYARERGIDALFALG
DASRDACTAYGDTARHFGDVGALLTALLAAGYGAQATVLVKGSRYMKMERVVDALTNQSAAGTVPAAH

Sequences:

>Translated_468_residues
MTMLSLGEAARLIPGATVHGDPDTTFERVSTDSRTVGPGDLFVALKGDRFDAHDFLGDVAARGAAAALVAHVPAGLAMPV
VEGGETRAALGALAHGWRKRFAVPLVAVTGSNGKTTVKEMIASIFAAAVGADARLATAGNLNNDIGVPLTLLRLSGAHRV
AVIELGMNHPGETEILARLAAPTVALVNNAQREHQEFMATVEAVALEHAAVIHALTPDGVAVFPADDAYASIWRVAATGN
PILDFALHDGERQVEAQVTGRLHGGELAIDTPSGAVTVRLRALGEHNARNALAAAAAALAAGIELAAIKQGLESFEPVKG
RLQVRQADVGSLAGATVVDDTYNANPDSMRAAIDVLAAQPAPRVLVIGDMGEVGDEGPAFHREIGAYARERGIDALFALG
DASRDACTAYGDTARHFGDVGALLTALLAAGYGAQATVLVKGSRYMKMERVVDALTNQSAAGTVPAAH
>Mature_467_residues
TMLSLGEAARLIPGATVHGDPDTTFERVSTDSRTVGPGDLFVALKGDRFDAHDFLGDVAARGAAAALVAHVPAGLAMPVV
EGGETRAALGALAHGWRKRFAVPLVAVTGSNGKTTVKEMIASIFAAAVGADARLATAGNLNNDIGVPLTLLRLSGAHRVA
VIELGMNHPGETEILARLAAPTVALVNNAQREHQEFMATVEAVALEHAAVIHALTPDGVAVFPADDAYASIWRVAATGNP
ILDFALHDGERQVEAQVTGRLHGGELAIDTPSGAVTVRLRALGEHNARNALAAAAAALAAGIELAAIKQGLESFEPVKGR
LQVRQADVGSLAGATVVDDTYNANPDSMRAAIDVLAAQPAPRVLVIGDMGEVGDEGPAFHREIGAYARERGIDALFALGD
ASRDACTAYGDTARHFGDVGALLTALLAAGYGAQATVLVKGSRYMKMERVVDALTNQSAAGTVPAAH

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=437, Percent_Identity=43.2494279176201, Blast_Score=265, Evalue=4e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48176; Mature: 48045

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLSLGEAARLIPGATVHGDPDTTFERVSTDSRTVGPGDLFVALKGDRFDAHDFLGDVA
CCCCCCCCHHHHCCCCEECCCCCCHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
ARGAAAALVAHVPAGLAMPVVEGGETRAALGALAHGWRKRFAVPLVAVTGSNGKTTVKEM
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHH
IASIFAAAVGADARLATAGNLNNDIGVPLTLLRLSGAHRVAVIELGMNHPGETEILARLA
HHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHHH
APTVALVNNAQREHQEFMATVEAVALEHAAVIHALTPDGVAVFPADDAYASIWRVAATGN
CCHHHHHHCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCHHHHHEEEEECCC
PILDFALHDGERQVEAQVTGRLHGGELAIDTPSGAVTVRLRALGEHNARNALAAAAAALA
CEEEEEECCCCCEEEEEEEEEECCCEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHH
AGIELAAIKQGLESFEPVKGRLQVRQADVGSLAGATVVDDTYNANPDSMRAAIDVLAAQP
HCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCC
APRVLVIGDMGEVGDEGPAFHREIGAYARERGIDALFALGDASRDACTAYGDTARHFGDV
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCHHHHHHHH
GALLTALLAAGYGAQATVLVKGSRYMKMERVVDALTNQSAAGTVPAAH
HHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TMLSLGEAARLIPGATVHGDPDTTFERVSTDSRTVGPGDLFVALKGDRFDAHDFLGDVA
CCCCCCCHHHHCCCCEECCCCCCHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
ARGAAAALVAHVPAGLAMPVVEGGETRAALGALAHGWRKRFAVPLVAVTGSNGKTTVKEM
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHH
IASIFAAAVGADARLATAGNLNNDIGVPLTLLRLSGAHRVAVIELGMNHPGETEILARLA
HHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHHH
APTVALVNNAQREHQEFMATVEAVALEHAAVIHALTPDGVAVFPADDAYASIWRVAATGN
CCHHHHHHCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCHHHHHEEEEECCC
PILDFALHDGERQVEAQVTGRLHGGELAIDTPSGAVTVRLRALGEHNARNALAAAAAALA
CEEEEEECCCCCEEEEEEEEEECCCEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHH
AGIELAAIKQGLESFEPVKGRLQVRQADVGSLAGATVVDDTYNANPDSMRAAIDVLAAQP
HCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCC
APRVLVIGDMGEVGDEGPAFHREIGAYARERGIDALFALGDASRDACTAYGDTARHFGDV
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCHHHHHHHH
GALLTALLAAGYGAQATVLVKGSRYMKMERVVDALTNQSAAGTVPAAH
HHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]