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The map label for this gene is hisF [H]

Identifier: 115350404

GI number: 115350404

Start: 392700

End: 393473

Strand: Direct

Name: hisF [H]

Synonym: Bamb_0349

Alternate gene names: 115350404

Gene position: 392700-393473 (Clockwise)

Preceding gene: 115350403

Following gene: 115350405

Centisome position: 11.04

GC content: 68.86

Gene sequence:

>774_bases
ATGGCTCTAGCTAAACGCATCATCCCCTGCCTGGACGTGACCGCCGGGCGCGTCGTCAAGGGCGTCAATTTCGTCGAGCT
GCGCGATGCCGGCGACCCCGTCGAAATCGCCCGCCGCTATGACGACCAGGGCGCCGACGAACTGACGTTCCTCGACATCA
CCGCGACCTCCGACCAGCGCGACCTGATCCTGCCGATCATCGAAGCCGTCGCGTCGCAGGTGTTCATTCCGCTGACCGTC
GGCGGCGGCGTGCGCGCCGTCGAGGACGTGCGGCGCCTGCTGAACGCGGGCGCGGACAAGGTCAGCATGAATTCGTCGGC
GGTCGCGAATCCGCAGCTCGTACGCGACGCGGCCGACAAGTACGGCTCGCAGTGCATCGTCGTCGCGATCGACGCGAAGC
GCGTGTCGGCCGACGGCGAGACGCCGCGCTGGGAAGTGTTCACGCACGGCGGCCGCAAGAACACGGGCCTCGACGCGATC
GAATGGGCGCGCAAGATGGCCGAGCTCGGCGCGGGCGAGATTCTGCTCACGAGCATGGATCGCGACGGCACGAAGTCGGG
CTTCGACCTCGCGCTCACGCGCGGCGTGTCGGACGCGGTGCCGGTGCCGGTGATCGCGTCGGGCGGCGTCGGCTCGCTGC
AGCACCTGGCCGACGGCATCAAGGACGGCCGCGCTGACGCGGTGCTGGCCGCCAGCATCTTCCACTACGGCGAGCACACG
GTCGGCGAGGCGAAGCGCTTCATGTCCGATCAGGGCATTCCGGTGAGGCTGTGA

Upstream 100 bases:

>100_bases
TTGTCCCGTTCGCCGGGGCTGCCTGCTGGCGGCCCCGACCGGAAGCCTCGCGCGAGGCTTCCGTAACCGGCCGTCCCATC
GCGGCCTTTGGCGCAACATC

Downstream 100 bases:

>100_bases
TGAATACGGAAACGAAAGCGCTGCCCGCGTGGCTCGACAAGGTCCGCTGGGACGACAACGGCCTCGTGCCGGTGATCGCG
CAGGAAGCATCGACGAACGA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQRDLILPIIEAVASQVFIPLTV
GGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADKYGSQCIVVAIDAKRVSADGETPRWEVFTHGGRKNTGLDAI
EWARKMAELGAGEILLTSMDRDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLQHLADGIKDGRADAVLAASIFHYGEHT
VGEAKRFMSDQGIPVRL

Sequences:

>Translated_257_residues
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQRDLILPIIEAVASQVFIPLTV
GGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADKYGSQCIVVAIDAKRVSADGETPRWEVFTHGGRKNTGLDAI
EWARKMAELGAGEILLTSMDRDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLQHLADGIKDGRADAVLAASIFHYGEHT
VGEAKRFMSDQGIPVRL
>Mature_256_residues
ALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQRDLILPIIEAVASQVFIPLTVG
GGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADKYGSQCIVVAIDAKRVSADGETPRWEVFTHGGRKNTGLDAIE
WARKMAELGAGEILLTSMDRDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLQHLADGIKDGRADAVLAASIFHYGEHTV
GEAKRFMSDQGIPVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=259, Percent_Identity=42.8571428571429, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI87082028, Length=242, Percent_Identity=31.404958677686, Blast_Score=102, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319725, Length=316, Percent_Identity=35.126582278481, Blast_Score=172, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 27277; Mature: 27146

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQR
CCHHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCC
DLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADK
CHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHH
YGSQCIVVAIDAKRVSADGETPRWEVFTHGGRKNTGLDAIEWARKMAELGAGEILLTSMD
HCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
RDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLQHLADGIKDGRADAVLAASIFHYGEHT
CCCCCCCCCEEEECCCCCCCCCCEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
VGEAKRFMSDQGIPVRL
HHHHHHHHCCCCCEECC
>Mature Secondary Structure 
ALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQR
CHHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCC
DLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADK
CHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHH
YGSQCIVVAIDAKRVSADGETPRWEVFTHGGRKNTGLDAIEWARKMAELGAGEILLTSMD
HCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
RDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLQHLADGIKDGRADAVLAASIFHYGEHT
CCCCCCCCCEEEECCCCCCCCCCEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
VGEAKRFMSDQGIPVRL
HHHHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA