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The map label for this gene is mlaE [H]

Identifier: 115350388

GI number: 115350388

Start: 379527

End: 380294

Strand: Direct

Name: mlaE [H]

Synonym: Bamb_0333

Alternate gene names: 115350388

Gene position: 379527-380294 (Clockwise)

Preceding gene: 115350387

Following gene: 115350389

Centisome position: 10.67

GC content: 67.06

Gene sequence:

>768_bases
ATGATCAGCGCGATCGGACGTTACGTCATCGGCGGCCTCGAGCGCGCGGGCTACGGCACGCGGCTGTTCGTGCGCCTCGT
GCTGGAATTCTTCCCGCTGCTGCGCCGGCCGCGGCTGGTCACGAAGCAGATCCACTTCCTCGGCAATTATTCGTTCGTGA
TCATTGCCGTGTCGGGCCTGTTCGTCGGCTTCGTGCTCGGCCTGCAGGGCTACTACACGCTGAACCGCTACGGGTCCGAG
CAGGCGCTCGGCCTGCTGGTCGCGCTGTCGCTCGTGCGCGAGCTCGGCCCGGTCGTGACCGCGCTGCTGTTCGCGGGCCG
CGCGGGCACGTCGCTCACGGCCGAGATCGGCCTGATGAAGGCCGGCGAGCAGCTCACCGCGCTCGAGATGATGGCCGTCG
ACCCGATCAAGACGGTGATCGCGCCGCGCCTGTGGGCCGGCATCATCGCGATGCCGCTGCTCGCCGCGATCTTCAACGCG
GTCGGCGTGCTCGGCGGGTATTTCGTCGGCGTCGTGCTGATCGGCGTCGATCCGGGCGCGTTCTGGTCGCAGATGCAGGG
CGGGGTCCAGGTGTGGGCCGACGTCGGCAACGGCGTGATCAAGAGCATCGTGTTCGGGTTCGCCGTGACCTTCATTGCAC
TGTTTCAAGGGTATGAAGCGAAGCCCACGCCCGAGGGCGTGTCGCGCGCGACGACCAAGACGGTCGTGTTCGCGTCGCTC
GCCGTACTCGGCCTCGATTTCCTGCTGACCGCGCTGATGTTCAGCTAA

Upstream 100 bases:

>100_bases
GCGTGCGGCAGTTCATCGACGGCGCGCCGGACGGCCCGTTCAAATTTCATTACACGAGCCCGCCGCTGGCAGCGGATTTC
GGGCTCGGCGGAGGGCGCGC

Downstream 100 bases:

>100_bases
GCCTCAGCCAAGCCAAATTTTGGGATGACGATGAAAAAGACTGCTCTCGACTTCTGGGTCGGCCTGTTCGTGGTGGTGGG
CTTCCTTGCGGTGCTGTTCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MISAIGRYVIGGLERAGYGTRLFVRLVLEFFPLLRRPRLVTKQIHFLGNYSFVIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKAGEQLTALEMMAVDPIKTVIAPRLWAGIIAMPLLAAIFNA
VGVLGGYFVGVVLIGVDPGAFWSQMQGGVQVWADVGNGVIKSIVFGFAVTFIALFQGYEAKPTPEGVSRATTKTVVFASL
AVLGLDFLLTALMFS

Sequences:

>Translated_255_residues
MISAIGRYVIGGLERAGYGTRLFVRLVLEFFPLLRRPRLVTKQIHFLGNYSFVIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKAGEQLTALEMMAVDPIKTVIAPRLWAGIIAMPLLAAIFNA
VGVLGGYFVGVVLIGVDPGAFWSQMQGGVQVWADVGNGVIKSIVFGFAVTFIALFQGYEAKPTPEGVSRATTKTVVFASL
AVLGLDFLLTALMFS
>Mature_255_residues
MISAIGRYVIGGLERAGYGTRLFVRLVLEFFPLLRRPRLVTKQIHFLGNYSFVIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKAGEQLTALEMMAVDPIKTVIAPRLWAGIIAMPLLAAIFNA
VGVLGGYFVGVVLIGVDPGAFWSQMQGGVQVWADVGNGVIKSIVFGFAVTFIALFQGYEAKPTPEGVSRATTKTVVFASL
AVLGLDFLLTALMFS

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=220, Percent_Identity=61.3636363636364, Blast_Score=268, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27185; Mature: 27185

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]