Click here to switch to the map view.

The map label for this gene is pilQ [H]

Identifier: 115350365

GI number: 115350365

Start: 350309

End: 351970

Strand: Direct

Name: pilQ [H]

Synonym: Bamb_0310

Alternate gene names: 115350365

Gene position: 350309-351970 (Clockwise)

Preceding gene: 115350364

Following gene: 115350366

Centisome position: 9.85

GC content: 70.88

Gene sequence:

>1662_bases
ATGACGAAATACGGATGGTGGGCGATCGCATTCCAGGTCGCATCATGCATGGCCGCGGCACACGCGGCGCTACCGCCTTT
GCCTCCGTTGCCCCCTTTGCCGCCGGCCGGCTGGTCGATCGCATCGATGCAGGCGTCCGTTGAGGACACACCGCTGCCGC
ACGGCGCGCTCGATGGCGCGGACGGCATGAATGGTGCCGCCTCCACACAGGACGGCCCACAGCCGTTCGATCGGGCCGCG
CGTGCAGCCGTTCGGGCAGGCACGACCGCGCCGGTACCGTTGCTGGAAGGGCCGCCGATCCCCTTGCCGCCGCCGACGCG
CATGAGCGACGCCGCGGCGCGGCACCCCGGCGATGCGGACGACGCGCGGCGGATCTCGCTGAACCTGCAAGGCGCCGGGC
TCGCGGCTGCATTCGATGCGCTCGCACGATTCACCGGGCTCAACATCATCGTCGGTGAACAGGTGCGCGGCACCGTGACG
TTACGTCTGAACAATGTCCGCTGGCGCGATGCGTTCGATACGTTGCTCGATACGCACGGGCTGGCCATGTCGCGGCGCGG
CAACGTGATCTGGGTCACGCCGGCGGCCGAACTCGCGGCGCGGGAGCGCGAGCGCTTCGAAACGCACGCCCGCGCGGCCG
AGCTGGAGCCGCTCGCGAGCCGGACCTTCGCGCTGCATTACCCGCGCGCGCTGGACGTGCAGCGGCTGCTGGCCGGTGCG
ACCGGCCAGCGCCTGCTGTCGAAACGCGGCGCGGCGGCGGCCGATCCGCGCACGAACCTGCTGTTCGTCACCGATCTTGC
GCCGCGCATCGCGCAGATCGCGGGGCTGATCGACGCGATCGACCGGCCGTCGCGACAGGTGCGGATCGAGGCGCGCATCG
TCGAAGGCGAACAGGGCTTCTCGCGTAACCTCGGTGCTCGCATCGCGTTGCGCGCGCAGGGACGGGCGCCCACCGCCGAC
GGCGCGGCATCCGCGACCGACACGCGCAACGCGCTCGATCTCGCCGCGCGGCCGCTCGGCGGCTTCGAGGCGGCGACGGC
CGGCTTCACGTTGTTCGCCGCGCCGCTGAGCCGCGTGCTCGACGTCGAGCTGAGCGCGCTCGAGGCGCAGGGGCGGGGCC
AGATCGTCTCCAGCCCGCGGGTGGTCACGGCCGACCGCGTGAAGGCGATCGTCGAACAGGGCTCGGAGCTGCCGTATCAG
GCCAAGGTCGGCAATGGCGTGAGCGGCGTGCAGTTCCGCCGCGCGACACTGAAGCTCGAGGTCGAGCCGCAGATCACGCC
CGACGGGCGGGTCGTGCTCGACCTCGACGTGACGAAGGACAGCATCGGCGAGCCGACCGCGGCCGGCCCCGCGATCCATA
CGAAGCACGTCCAGACGCGCGTGGAGGTCGAAAACGGCGGGACGGTCGCGATCGGCGGGATTTACGAGCAGTTGAACCGG
GATGATGTGACGCGGGTGCCGCTCTTGGGCAAAATACCGGTACTGGGCGCGCTTTTCCGGCACCGCGCGCGGCGCGACCA
GCGCAGCGAACTGGTCGTCTTCATCACGCCGACGGTCGTCGGCACGCAGTGCGCGACGTCCAGCGCGGGCGATGCGGACG
CGGGAAAAAGGGGGCCGGAAGCGGGTGGCGCAGGCTCGACAAGGCAGCCGCTTTGCCAGTAA

Upstream 100 bases:

>100_bases
CGCTTGGGGCCGCGCGGGTGATGCGCGTCGAAGCCGATGCCGTAACGCTTGCAACGGCCGATGGCGCGCGCCGGTTCGTC
ATGGACGACGGAGGTCGTCC

Downstream 100 bases:

>100_bases
GCTGCGCCACACACCAGCAAGATTCAAGCAGAGGAAGCCGTTGCAAGCGCGGGACCCACACGCAAACGTATTTTTCGTCG
GCCTTATGGGAGCGGGTAAG

Product: type IV pilus secretin PilQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MTKYGWWAIAFQVASCMAAAHAALPPLPPLPPLPPAGWSIASMQASVEDTPLPHGALDGADGMNGAASTQDGPQPFDRAA
RAAVRAGTTAPVPLLEGPPIPLPPPTRMSDAAARHPGDADDARRISLNLQGAGLAAAFDALARFTGLNIIVGEQVRGTVT
LRLNNVRWRDAFDTLLDTHGLAMSRRGNVIWVTPAAELAARERERFETHARAAELEPLASRTFALHYPRALDVQRLLAGA
TGQRLLSKRGAAAADPRTNLLFVTDLAPRIAQIAGLIDAIDRPSRQVRIEARIVEGEQGFSRNLGARIALRAQGRAPTAD
GAASATDTRNALDLAARPLGGFEAATAGFTLFAAPLSRVLDVELSALEAQGRGQIVSSPRVVTADRVKAIVEQGSELPYQ
AKVGNGVSGVQFRRATLKLEVEPQITPDGRVVLDLDVTKDSIGEPTAAGPAIHTKHVQTRVEVENGGTVAIGGIYEQLNR
DDVTRVPLLGKIPVLGALFRHRARRDQRSELVVFITPTVVGTQCATSSAGDADAGKRGPEAGGAGSTRQPLCQ

Sequences:

>Translated_553_residues
MTKYGWWAIAFQVASCMAAAHAALPPLPPLPPLPPAGWSIASMQASVEDTPLPHGALDGADGMNGAASTQDGPQPFDRAA
RAAVRAGTTAPVPLLEGPPIPLPPPTRMSDAAARHPGDADDARRISLNLQGAGLAAAFDALARFTGLNIIVGEQVRGTVT
LRLNNVRWRDAFDTLLDTHGLAMSRRGNVIWVTPAAELAARERERFETHARAAELEPLASRTFALHYPRALDVQRLLAGA
TGQRLLSKRGAAAADPRTNLLFVTDLAPRIAQIAGLIDAIDRPSRQVRIEARIVEGEQGFSRNLGARIALRAQGRAPTAD
GAASATDTRNALDLAARPLGGFEAATAGFTLFAAPLSRVLDVELSALEAQGRGQIVSSPRVVTADRVKAIVEQGSELPYQ
AKVGNGVSGVQFRRATLKLEVEPQITPDGRVVLDLDVTKDSIGEPTAAGPAIHTKHVQTRVEVENGGTVAIGGIYEQLNR
DDVTRVPLLGKIPVLGALFRHRARRDQRSELVVFITPTVVGTQCATSSAGDADAGKRGPEAGGAGSTRQPLCQ
>Mature_552_residues
TKYGWWAIAFQVASCMAAAHAALPPLPPLPPLPPAGWSIASMQASVEDTPLPHGALDGADGMNGAASTQDGPQPFDRAAR
AAVRAGTTAPVPLLEGPPIPLPPPTRMSDAAARHPGDADDARRISLNLQGAGLAAAFDALARFTGLNIIVGEQVRGTVTL
RLNNVRWRDAFDTLLDTHGLAMSRRGNVIWVTPAAELAARERERFETHARAAELEPLASRTFALHYPRALDVQRLLAGAT
GQRLLSKRGAAAADPRTNLLFVTDLAPRIAQIAGLIDAIDRPSRQVRIEARIVEGEQGFSRNLGARIALRAQGRAPTADG
AASATDTRNALDLAARPLGGFEAATAGFTLFAAPLSRVLDVELSALEAQGRGQIVSSPRVVTADRVKAIVEQGSELPYQA
KVGNGVSGVQFRRATLKLEVEPQITPDGRVVLDLDVTKDSIGEPTAAGPAIHTKHVQTRVEVENGGTVAIGGIYEQLNRD
DVTRVPLLGKIPVLGALFRHRARRDQRSELVVFITPTVVGTQCATSSAGDADAGKRGPEAGGAGSTRQPLCQ

Specific function: Required for type IV pilus biogenesis and competence. Could function as a pore for exit of the pilus but also as a channel for entry of heme and antimicrobial agents and uptake of transforming DNA [H]

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI1789793, Length=410, Percent_Identity=33.9024390243902, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI87082242, Length=358, Percent_Identity=26.536312849162, Blast_Score=113, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR013355
- InterPro:   IPR011662
- InterPro:   IPR004846
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N; PF07660 STN [H]

EC number: NA

Molecular weight: Translated: 58298; Mature: 58167

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYGWWAIAFQVASCMAAAHAALPPLPPLPPLPPAGWSIASMQASVEDTPLPHGALDGA
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHEECCCCCCCCCCCCCCCC
DGMNGAASTQDGPQPFDRAARAAVRAGTTAPVPLLEGPPIPLPPPTRMSDAAARHPGDAD
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC
DARRISLNLQGAGLAAAFDALARFTGLNIIVGEQVRGTVTLRLNNVRWRDAFDTLLDTHG
CCEEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCEEHHHHHHHHHHHCC
LAMSRRGNVIWVTPAAELAARERERFETHARAAELEPLASRTFALHYPRALDVQRLLAGA
CEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEECCCHHHHHHHHCCC
TGQRLLSKRGAAAADPRTNLLFVTDLAPRIAQIAGLIDAIDRPSRQVRIEARIVEGEQGF
HHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCC
SRNLGARIALRAQGRAPTADGAASATDTRNALDLAARPLGGFEAATAGFTLFAAPLSRVL
CCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHH
DVELSALEAQGRGQIVSSPRVVTADRVKAIVEQGSELPYQAKVGNGVSGVQFRRATLKLE
HHHHHHHCCCCCCCEECCCCEEEHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEEEEEE
VEPQITPDGRVVLDLDVTKDSIGEPTAAGPAIHTKHVQTRVEVENGGTVAIGGIYEQLNR
ECCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEEEEECCCCEEEECHHHHHCCC
DDVTRVPLLGKIPVLGALFRHRARRDQRSELVVFITPTVVGTQCATSSAGDADAGKRGPE
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCEECCCCCCCCCCCCCCCCCCCC
AGGAGSTRQPLCQ
CCCCCCCCCCCCC
>Mature Secondary Structure 
TKYGWWAIAFQVASCMAAAHAALPPLPPLPPLPPAGWSIASMQASVEDTPLPHGALDGA
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHEECCCCCCCCCCCCCCCC
DGMNGAASTQDGPQPFDRAARAAVRAGTTAPVPLLEGPPIPLPPPTRMSDAAARHPGDAD
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC
DARRISLNLQGAGLAAAFDALARFTGLNIIVGEQVRGTVTLRLNNVRWRDAFDTLLDTHG
CCEEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCEEHHHHHHHHHHHCC
LAMSRRGNVIWVTPAAELAARERERFETHARAAELEPLASRTFALHYPRALDVQRLLAGA
CEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEECCCHHHHHHHHCCC
TGQRLLSKRGAAAADPRTNLLFVTDLAPRIAQIAGLIDAIDRPSRQVRIEARIVEGEQGF
HHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCC
SRNLGARIALRAQGRAPTADGAASATDTRNALDLAARPLGGFEAATAGFTLFAAPLSRVL
CCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHH
DVELSALEAQGRGQIVSSPRVVTADRVKAIVEQGSELPYQAKVGNGVSGVQFRRATLKLE
HHHHHHHCCCCCCCEECCCCEEEHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEEEEEE
VEPQITPDGRVVLDLDVTKDSIGEPTAAGPAIHTKHVQTRVEVENGGTVAIGGIYEQLNR
ECCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEEEEECCCCEEEECHHHHHCCC
DDVTRVPLLGKIPVLGALFRHRARRDQRSELVVFITPTVVGTQCATSSAGDADAGKRGPE
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCEECCCCCCCCCCCCCCCCCCCC
AGGAGSTRQPLCQ
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]