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The map label for this gene is fixJ [H]

Identifier: 116249452

GI number: 116249452

Start: 861948

End: 862325

Strand: Reverse

Name: fixJ [H]

Synonym: pRL120791

Alternate gene names: 116249452

Gene position: 862325-861948 (Counterclockwise)

Preceding gene: 116249453

Following gene: 116249451

Centisome position: 99.12

GC content: 58.2

Gene sequence:

>378_bases
ATGAACAAGACAAGACACGTCGTGGCAATTGTCGATGACGATCCAAGGTTGCTTGAATCGATGGGCGACCTTCTGGAATC
TGCGGGTTATGTGGCCCGCAGTTTCTCATCGGCGGGCTCGCTGCTCGTCAACGGGCTGTCGGACCTCGACCTGCTCATCA
CCGATATCGGAATGCCTGGTATCGACGGCTTCGAACTTCGTGACCTGGTGAGGAAGTCGCGTCCGGAGCTGCCGGTATTC
ATGATCACGGGCCGCCACGAGATAGCCGATCAGGGACGCGCTCAAGGCGCAAGCGGATTTTTCCGCAAGCCCTTCGATGC
CCAGGCCCTGCTGGCGGCCATTGCCAACGCTTTGCATAAATCGACAGATGGAGGCTGA

Upstream 100 bases:

>100_bases
GCAGAAAATGGCCGCGGCATCGCTCGCCGATCTGGTGCGGATCGCGGAGAGATTGGAAATACCGATAACCCACTCGCGCC
GAGTGGGAGGGAATTGAAAC

Downstream 100 bases:

>100_bases
CATGAGAGTTGACCAGCCGTTGCTGCGGAGATCGGCGCCGTTATCATTGCAATGCAGTTATGAAGACAACCGGCCGCTTG
TCATTATCGTCGACGACGAC

Product: putative two-component response regulator transcriptional regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MNKTRHVVAIVDDDPRLLESMGDLLESAGYVARSFSSAGSLLVNGLSDLDLLITDIGMPGIDGFELRDLVRKSRPELPVF
MITGRHEIADQGRAQGASGFFRKPFDAQALLAAIANALHKSTDGG

Sequences:

>Translated_125_residues
MNKTRHVVAIVDDDPRLLESMGDLLESAGYVARSFSSAGSLLVNGLSDLDLLITDIGMPGIDGFELRDLVRKSRPELPVF
MITGRHEIADQGRAQGASGFFRKPFDAQALLAAIANALHKSTDGG
>Mature_125_residues
MNKTRHVVAIVDDDPRLLESMGDLLESAGYVARSFSSAGSLLVNGLSDLDLLITDIGMPGIDGFELRDLVRKSRPELPVF
MITGRHEIADQGRAQGASGFFRKPFDAQALLAAIANALHKSTDGG

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=123, Percent_Identity=39.0243902439024, Blast_Score=60, Evalue=3e-11,
Organism=Escherichia coli, GI1790299, Length=117, Percent_Identity=31.6239316239316, Blast_Score=60, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 13395; Mature: 13395

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTRHVVAIVDDDPRLLESMGDLLESAGYVARSFSSAGSLLVNGLSDLDLLITDIGMPG
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
IDGFELRDLVRKSRPELPVFMITGRHEIADQGRAQGASGFFRKPFDAQALLAAIANALHK
CCCHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
STDGG
CCCCC
>Mature Secondary Structure
MNKTRHVVAIVDDDPRLLESMGDLLESAGYVARSFSSAGSLLVNGLSDLDLLITDIGMPG
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
IDGFELRDLVRKSRPELPVFMITGRHEIADQGRAQGASGFFRKPFDAQALLAAIANALHK
CCCHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
STDGG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]