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The map label for this gene is nodW [H]

Identifier: 116249451

GI number: 116249451

Start: 861254

End: 861946

Strand: Reverse

Name: nodW [H]

Synonym: pRL120790

Alternate gene names: 116249451

Gene position: 861946-861254 (Counterclockwise)

Preceding gene: 116249452

Following gene: 116249450

Centisome position: 99.07

GC content: 61.9

Gene sequence:

>693_bases
ATGAGAGTTGACCAGCCGTTGCTGCGGAGATCGGCGCCGTTATCATTGCAATGCAGTTATGAAGACAACCGGCCGCTTGT
CATTATCGTCGACGACGACGCATCGGTCCGCGAAGCGCTGTCGGAGCTGATCCAGTCGGCGGGTTTCCAGTCGACCAGTT
TTGCTTCGACCCGAGAATTGCTTGACGCCGATATACTGGATAGCCCCGGCTGCCTGATCCTCGACGTGCGCATGCCAGGC
GCAAGCGGCCTTCACCTGCAGAGCCATCTGGCCGAAAACGGCATTTCCAAGCCGATCATCTTCCTGACCGGGCATGGCGA
CATCCCGATGACCGTCCAGGCGATGAAGGCCGGTGCCGTGGATTTTCTCACCAAACCGGTGCGAGACCAGACCCTGCTCG
ACGCCGTGACTGCCGGCATTGCGATGGATGCCGAGCGACGGGCGGAAGCCGCGATCGCCGAGCGCAATATCAAGCGCCTG
GAGACACTGACGCAGCGCGAGCGCGAGGTCCTGCATGAAGTGGCGCGCGGCCGCCTCAACAAGCAGATCGCCTTCGACCT
CGGCATCAGCGAAGTGACGGTCAAGCTGCATCGCAGCAATGTCATGCACAAGATGGAGGCGGCCTCCATCGGCGAACTGA
TCCGGGCCTGGGAAACGCTGCCCACGCAAATGCGCCAGGTCGGCCCGCGATAG

Upstream 100 bases:

>100_bases
GCTCAAGGCGCAAGCGGATTTTTCCGCAAGCCCTTCGATGCCCAGGCCCTGCTGGCGGCCATTGCCAACGCTTTGCATAA
ATCGACAGATGGAGGCTGAC

Downstream 100 bases:

>100_bases
CTTATAGGCGAAGGATGCCGGCCTCGAGAATGATCTGCCGTCGCTGTCCATTTGATGCGCGCCGTATCCGATTCGCGCCG
GAAAAGACGATGCGGCGCCG

Product: putative NodW-like nodulation transcriptional regulatory protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MRVDQPLLRRSAPLSLQCSYEDNRPLVIIVDDDASVREALSELIQSAGFQSTSFASTRELLDADILDSPGCLILDVRMPG
ASGLHLQSHLAENGISKPIIFLTGHGDIPMTVQAMKAGAVDFLTKPVRDQTLLDAVTAGIAMDAERRAEAAIAERNIKRL
ETLTQREREVLHEVARGRLNKQIAFDLGISEVTVKLHRSNVMHKMEAASIGELIRAWETLPTQMRQVGPR

Sequences:

>Translated_230_residues
MRVDQPLLRRSAPLSLQCSYEDNRPLVIIVDDDASVREALSELIQSAGFQSTSFASTRELLDADILDSPGCLILDVRMPG
ASGLHLQSHLAENGISKPIIFLTGHGDIPMTVQAMKAGAVDFLTKPVRDQTLLDAVTAGIAMDAERRAEAAIAERNIKRL
ETLTQREREVLHEVARGRLNKQIAFDLGISEVTVKLHRSNVMHKMEAASIGELIRAWETLPTQMRQVGPR
>Mature_230_residues
MRVDQPLLRRSAPLSLQCSYEDNRPLVIIVDDDASVREALSELIQSAGFQSTSFASTRELLDADILDSPGCLILDVRMPG
ASGLHLQSHLAENGISKPIIFLTGHGDIPMTVQAMKAGAVDFLTKPVRDQTLLDAVTAGIAMDAERRAEAAIAERNIKRL
ETLTQREREVLHEVARGRLNKQIAFDLGISEVTVKLHRSNVMHKMEAASIGELIRAWETLPTQMRQVGPR

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=105, Percent_Identity=37.1428571428571, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788521, Length=199, Percent_Identity=33.6683417085427, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790102, Length=198, Percent_Identity=32.3232323232323, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 25354; Mature: 25354

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDQPLLRRSAPLSLQCSYEDNRPLVIIVDDDASVREALSELIQSAGFQSTSFASTREL
CCCCHHHHHCCCCCEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
LDADILDSPGCLILDVRMPGASGLHLQSHLAENGISKPIIFLTGHGDIPMTVQAMKAGAV
HHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCHH
DFLTKPVRDQTLLDAVTAGIAMDAERRAEAAIAERNIKRLETLTQREREVLHEVARGRLN
HHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KQIAFDLGISEVTVKLHRSNVMHKMEAASIGELIRAWETLPTQMRQVGPR
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRVDQPLLRRSAPLSLQCSYEDNRPLVIIVDDDASVREALSELIQSAGFQSTSFASTREL
CCCCHHHHHCCCCCEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
LDADILDSPGCLILDVRMPGASGLHLQSHLAENGISKPIIFLTGHGDIPMTVQAMKAGAV
HHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCHH
DFLTKPVRDQTLLDAVTAGIAMDAERRAEAAIAERNIKRLETLTQREREVLHEVARGRLN
HHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KQIAFDLGISEVTVKLHRSNVMHKMEAASIGELIRAWETLPTQMRQVGPR
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]