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The map label for this gene is mhpD [H]

Identifier: 116249366

GI number: 116249366

Start: 762910

End: 763680

Strand: Reverse

Name: mhpD [H]

Synonym: pRL120704

Alternate gene names: 116249366

Gene position: 763680-762910 (Counterclockwise)

Preceding gene: 116249367

Following gene: 116249358

Centisome position: 87.78

GC content: 61.61

Gene sequence:

>771_bases
ATGTACTTCGGACTCAAAGACACCACGGCCGAAATCCTTGCCAAAAGTCGTCTGCATGATGCGCTTTGCGACCTGCCGCT
CGATCTGATCGACTCCGAGCGGGAGGCCTATGACATCCAGGCCCTAGCCCAGGACGCTCTCGGATTTGAGCGCAAGGGAT
ACGCTATCGTCGGATCCAGCGAGTCCGCCCGCCGTGCGCTGGGGCTCACCAAGCCGATTTATTCGGCGATCCCGGCTCTC
GCGCTTGCATCCGCAACGGCGGTGTTTCGATTGCCGCCGGGCGTCATCGGCGCCCAGTGCGAATTTACATTCACGATGCG
GCGAGCATTTCCTGATGCTGACGAGCCCATTTCGCTGGAAACCGTTGCCGATGCTGTTGCTGCATGCCGGCCGGCAATCG
GCATCATCGGACGTCGCACGGGTCGGGCGTTCGCCGGCGACAATTCCGCGATCGCAGATTTTGGTCTGCACGTCGCGACC
GTTCGGGGCAGTCAGACGGCTGAGGTCGATCTCTCCGCGCTTGGGAAGCTCGACGTCAATGTCTTCCTGTTCAGGGAGGT
CGGGATTTCGGGAAATGCATCATCTGTTATGGGGCATCCCCTGAACGCTGTGGCCTGGCTGGCTGGCGCCCTCTCCTCTC
GTGGCAAGCAACTTCAGACCGGCGATGTCGTCGCGACGGGCGCCTGCACGAGGGTCCTCCGCGTTCATGCGGGACAGCAT
GTTGCCGCCGATTTCGGGCCGCTGGGCCAAGTCGAATGCATTTTCGAGTAG

Upstream 100 bases:

>100_bases
TTTTAATCTCACGCGCCCGCCCAGCGCATGGAATAGCTATGGGCTGATCTCCATTCTTAACAGCATCCAAAACTTGCTGA
ACGGATATGGAGTTACTGTG

Downstream 100 bases:

>100_bases
CCGGGAGAGAAGGTTAGCATATGCGTCCCCAACAGAAACCATTCGTCGTGGAGATCAGAAAAAGTCGGCGCCGAAAGGAA
GCGTCGTCACCTTCATGCGA

Product: putative hydratase/decarboxylase

Products: NA

Alternate protein names: 2-hydroxypentadienoic acid hydratase [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MYFGLKDTTAEILAKSRLHDALCDLPLDLIDSEREAYDIQALAQDALGFERKGYAIVGSSESARRALGLTKPIYSAIPAL
ALASATAVFRLPPGVIGAQCEFTFTMRRAFPDADEPISLETVADAVAACRPAIGIIGRRTGRAFAGDNSAIADFGLHVAT
VRGSQTAEVDLSALGKLDVNVFLFREVGISGNASSVMGHPLNAVAWLAGALSSRGKQLQTGDVVATGACTRVLRVHAGQH
VAADFGPLGQVECIFE

Sequences:

>Translated_256_residues
MYFGLKDTTAEILAKSRLHDALCDLPLDLIDSEREAYDIQALAQDALGFERKGYAIVGSSESARRALGLTKPIYSAIPAL
ALASATAVFRLPPGVIGAQCEFTFTMRRAFPDADEPISLETVADAVAACRPAIGIIGRRTGRAFAGDNSAIADFGLHVAT
VRGSQTAEVDLSALGKLDVNVFLFREVGISGNASSVMGHPLNAVAWLAGALSSRGKQLQTGDVVATGACTRVLRVHAGQH
VAADFGPLGQVECIFE
>Mature_256_residues
MYFGLKDTTAEILAKSRLHDALCDLPLDLIDSEREAYDIQALAQDALGFERKGYAIVGSSESARRALGLTKPIYSAIPAL
ALASATAVFRLPPGVIGAQCEFTFTMRRAFPDADEPISLETVADAVAACRPAIGIIGRRTGRAFAGDNSAIADFGLHVAT
VRGSQTAEVDLSALGKLDVNVFLFREVGISGNASSVMGHPLNAVAWLAGALSSRGKQLQTGDVVATGACTRVLRVHAGQH
VAADFGPLGQVECIFE

Specific function: Catalyzes the conversion of 2-hydroxypentadienoic acid (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family. MhpD subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=161, Percent_Identity=29.8136645962733, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =4.2.1.80 [H]

Molecular weight: Translated: 26887; Mature: 26887

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFGLKDTTAEILAKSRLHDALCDLPLDLIDSEREAYDIQALAQDALGFERKGYAIVGSS
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEECC
ESARRALGLTKPIYSAIPALALASATAVFRLPPGVIGAQCEFTFTMRRAFPDADEPISLE
HHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEEEHHHCCCCCCCEEHH
TVADAVAACRPAIGIIGRRTGRAFAGDNSAIADFGLHVATVRGSQTAEVDLSALGKLDVN
HHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHCCEEEEEECCCCCCEEHHHHHCCCCCE
VFLFREVGISGNASSVMGHPLNAVAWLAGALSSRGKQLQTGDVVATGACTRVLRVHAGQH
EEEEEEECCCCCCCHHCCCCHHHHHHHHHHHHHCCCEECCCCEEEHHHHHHHHHHHCCCE
VAADFGPLGQVECIFE
EECCCCCCCCEEEEEC
>Mature Secondary Structure
MYFGLKDTTAEILAKSRLHDALCDLPLDLIDSEREAYDIQALAQDALGFERKGYAIVGSS
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEECC
ESARRALGLTKPIYSAIPALALASATAVFRLPPGVIGAQCEFTFTMRRAFPDADEPISLE
HHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEEEHHHCCCCCCCEEHH
TVADAVAACRPAIGIIGRRTGRAFAGDNSAIADFGLHVATVRGSQTAEVDLSALGKLDVN
HHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHCCEEEEEECCCCCCEEHHHHHCCCCCE
VFLFREVGISGNASSVMGHPLNAVAWLAGALSSRGKQLQTGDVVATGACTRVLRVHAGQH
EEEEEEECCCCCCCHHCCCCHHHHHHHHHHHHHCCCEECCCCEEEHHHHHHHHHHHCCCE
VAADFGPLGQVECIFE
EECCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA