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The map label for this gene is ycjZ [C]

Identifier: 116249212

GI number: 116249212

Start: 595761

End: 596657

Strand: Direct

Name: ycjZ [C]

Synonym: pRL120548

Alternate gene names: 116249212

Gene position: 595761-596657 (Clockwise)

Preceding gene: 116249208

Following gene: 116249215

Centisome position: 68.48

GC content: 60.87

Gene sequence:

>897_bases
ATGCGGCGCGACGAGTTCACGGAAATGCGTGCGTTTCTGGAGGTGGCTCAGGAGCGGAGTTTCACCCGCGCTGCATCGAA
ACTGGGAGTCACGCGCTCGGCGCTGAGCCATACGATCAGCGCGCTCGAAGCCCGGCTTGGCGTCCGGCTTCTCTCCCGCA
CGACGCGTGACGTGGCTCCCACGGCGGCCGGTGCCCGGCTCATGGAAAGTATTCAGCCGCATTTCGAGAGCATCGCCGCC
GGGATCAGTGCCCTCGGCGCGCTGCGCGACAAGCCTTCGGGCACAGTCCGCCTCGTATGTCCCGATGATGCCGTCGAGCT
GGTCTTCCGTTCGAGGCTGCCGGCATTTTTACGGGATTACCCCGATATCACCGTGGAACTCATCGTCGACAACGGTTTTA
CAAATATCGTCGAGCGCCAATTCGATGCCGGCGTGCGGCTCGGCGAAGCGATTGCGCGCGACATGGTGGCCGTTCGCATT
GGACCCGATATCTCTTACGCTGTGGTCGGATCAACCGATTATTTTGCGGCCCGCACTGTGCCTTCGACCCCGCATGACTT
GACGGACCACAATTGCGTCAACCTGCGTCTTCCGACGTCAGGCGCTCTCTATGCCTGGGAGTTTGAAAAGGATGGCCGCG
AGTTCAGCGTACGGGTGGAAGGCCAGCTCGTGATGAGCAACATAGGGCCGGCGATCAATGCCGCTCTCGATGGGGCGGGG
CTGTCTTATGTGCCGAGGGATCTGATCAGGCAATATTGTGACAGCGGTCAACTGCACGAAGTGCTGGCGGACTGGGCGCC
AACATTCCAAGGCTATCATCTCTATTACCCCAGCCGGCGCCATCCATCCCCTGCTTTCTCCGCCTTCGTTGAAGCTTTCC
GCTACCGGTATCGGTAG

Upstream 100 bases:

>100_bases
GGTCATGCATGTGCTCCTGTCCGCTGCCCGATAGATAGCCGGACTGGTTTTGCGCGACTAGAAGCTATAATCTGAACGTC
GTGATGAAGGAGATTCAACA

Downstream 100 bases:

>100_bases
CGGAAAACAGGCCGTGCGAAGCAGCGCAACCCGTCGCGCCTCTTGTTCATCGACAATTACCGGGCGCTGTCGATGATGGC
GCCACCGTCGGGGGTAAGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MRRDEFTEMRAFLEVAQERSFTRAASKLGVTRSALSHTISALEARLGVRLLSRTTRDVAPTAAGARLMESIQPHFESIAA
GISALGALRDKPSGTVRLVCPDDAVELVFRSRLPAFLRDYPDITVELIVDNGFTNIVERQFDAGVRLGEAIARDMVAVRI
GPDISYAVVGSTDYFAARTVPSTPHDLTDHNCVNLRLPTSGALYAWEFEKDGREFSVRVEGQLVMSNIGPAINAALDGAG
LSYVPRDLIRQYCDSGQLHEVLADWAPTFQGYHLYYPSRRHPSPAFSAFVEAFRYRYR

Sequences:

>Translated_298_residues
MRRDEFTEMRAFLEVAQERSFTRAASKLGVTRSALSHTISALEARLGVRLLSRTTRDVAPTAAGARLMESIQPHFESIAA
GISALGALRDKPSGTVRLVCPDDAVELVFRSRLPAFLRDYPDITVELIVDNGFTNIVERQFDAGVRLGEAIARDMVAVRI
GPDISYAVVGSTDYFAARTVPSTPHDLTDHNCVNLRLPTSGALYAWEFEKDGREFSVRVEGQLVMSNIGPAINAALDGAG
LSYVPRDLIRQYCDSGQLHEVLADWAPTFQGYHLYYPSRRHPSPAFSAFVEAFRYRYR
>Mature_298_residues
MRRDEFTEMRAFLEVAQERSFTRAASKLGVTRSALSHTISALEARLGVRLLSRTTRDVAPTAAGARLMESIQPHFESIAA
GISALGALRDKPSGTVRLVCPDDAVELVFRSRLPAFLRDYPDITVELIVDNGFTNIVERQFDAGVRLGEAIARDMVAVRI
GPDISYAVVGSTDYFAARTVPSTPHDLTDHNCVNLRLPTSGALYAWEFEKDGREFSVRVEGQLVMSNIGPAINAALDGAG
LSYVPRDLIRQYCDSGQLHEVLADWAPTFQGYHLYYPSRRHPSPAFSAFVEAFRYRYR

Specific function: May have a role in the regulation of oprD expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787589, Length=296, Percent_Identity=44.9324324324324, Blast_Score=286, Evalue=8e-79,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=38.0622837370242, Blast_Score=221, Evalue=5e-59,
Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=29.6551724137931, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI145693193, Length=295, Percent_Identity=28.4745762711864, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1789639, Length=291, Percent_Identity=24.7422680412371, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=25.6849315068493, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789440, Length=270, Percent_Identity=22.5925925925926, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=26.2975778546713, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788748, Length=118, Percent_Identity=33.8983050847458, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI145693105, Length=155, Percent_Identity=32.258064516129, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786713, Length=262, Percent_Identity=25.5725190839695, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788706, Length=303, Percent_Identity=23.4323432343234, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787879, Length=145, Percent_Identity=31.0344827586207, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788887, Length=254, Percent_Identity=29.1338582677165, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1790011, Length=278, Percent_Identity=23.021582733813, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33073; Mature: 33073

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRDEFTEMRAFLEVAQERSFTRAASKLGVTRSALSHTISALEARLGVRLLSRTTRDVAP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TAAGARLMESIQPHFESIAAGISALGALRDKPSGTVRLVCPDDAVELVFRSRLPAFLRDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCHHHHCC
PDITVELIVDNGFTNIVERQFDAGVRLGEAIARDMVAVRIGPDISYAVVGSTDYFAARTV
CCCEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHEEEEECCCCEEEEECCCCCEEECCC
PSTPHDLTDHNCVNLRLPTSGALYAWEFEKDGREFSVRVEGQLVMSNIGPAINAALDGAG
CCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEEECEEEECCCCCHHHHHHCCCC
LSYVPRDLIRQYCDSGQLHEVLADWAPTFQGYHLYYPSRRHPSPAFSAFVEAFRYRYR
CCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRRDEFTEMRAFLEVAQERSFTRAASKLGVTRSALSHTISALEARLGVRLLSRTTRDVAP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TAAGARLMESIQPHFESIAAGISALGALRDKPSGTVRLVCPDDAVELVFRSRLPAFLRDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCHHHHCC
PDITVELIVDNGFTNIVERQFDAGVRLGEAIARDMVAVRIGPDISYAVVGSTDYFAARTV
CCCEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHEEEEECCCCEEEEECCCCCEEECCC
PSTPHDLTDHNCVNLRLPTSGALYAWEFEKDGREFSVRVEGQLVMSNIGPAINAALDGAG
CCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEEECEEEECCCCCHHHHHHCCCC
LSYVPRDLIRQYCDSGQLHEVLADWAPTFQGYHLYYPSRRHPSPAFSAFVEAFRYRYR
CCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043; 1427017 [H]