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The map label for this gene is gcvA [H]

Identifier: 116249203

GI number: 116249203

Start: 585005

End: 585895

Strand: Direct

Name: gcvA [H]

Synonym: pRL120539

Alternate gene names: 116249203

Gene position: 585005-585895 (Clockwise)

Preceding gene: 116249200

Following gene: 116249206

Centisome position: 67.24

GC content: 62.51

Gene sequence:

>891_bases
GTGATGCACTTTAGAAGACGGATCCCGTCGCTGACGGCACTGGTGACCCTGGAGGCGGTGCTCAGAAAGAAGAGCTTCAC
CACGGCTGCGACCGAATTGGGTGTCACCCAGGCGGCGGTCAGCAGGCAGATTGCATTGCTGGAGGAGGAGTTCGGCCAGC
CCTTATTCGTGCGCAAGCATCGAGCGATCGAGCCGACGGCGGCATGCATCAACCTCGGAGCCACGCTGGCCAAGAGCTTT
GCCGATATTGCCGAGAGCGTGGAAGCGCTCCAGTCGCGCAGCCAGGACGTGTTGACCATAGGGGCGACCGTTGCATTCTC
TTCCTTCTGGCTGCTGCCGCGGCTGGCCGAATTCCGCCGGGCGAACCCGGGCATCCTGGTGCGGGTGATATCCCAGGACA
GCCCGATCGCTCTCGACGATGGAGAAGTCGATGTGGCAGTTCGATATGGTTTGCCCCCCTTCAGCGATAGCACCGTCATC
GCCTCGCACGGCGACGTCATCTGCCCCGTCTGCTCTCCCGACTATCTCAGGCGTCGCGGAGATGGCGCGCTCGGCTCCGC
CGACGAATTCATCGAAACGGACGTTCTCGATCGTTCCTGGTATAGCTGGGCGCAGTGGTTTTCGCTCACCGGCGCCAACC
TCGAGGTAAAGCCGTCGCTTCGGTTCAACCACTACACCGAAACCATCGCAGCCGCGCGCGCAGGGCAAGGGATTGCATTG
GGATGGCGTATGCTGGTGGGCACCTTTCTGGAGGATGGAACCCTGGTGCAGGTCGACGGCGGCGAGCTTGCCGCGGAGGA
TCGTTACAACGTGATCGTGCCCGTCAAAGCCAAGCGAAGCAACGCACGCGACCTTGCCGCCGCCTGGCTGACGGCATCAT
TGCACGGGTGA

Upstream 100 bases:

>100_bases
CTTATGCGAGAGTTTCCCGAGATTGCCCTTGCAGTGCCGTTGTTTCAACGGCATAAAAAATCAGGTTCGCATAAGTTATT
GTCATGCGATGGGGAGTTCT

Downstream 100 bases:

>100_bases
TCGAAGCGTAGTCACAAACCCAGCCCCACGGTTGCCGTTCGGTGAACGCCTTTAGGGACGAAGGCGAAGCAATCTGCCAG
TCCCAGGAAATCGGCCGCCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MMHFRRRIPSLTALVTLEAVLRKKSFTTAATELGVTQAAVSRQIALLEEEFGQPLFVRKHRAIEPTAACINLGATLAKSF
ADIAESVEALQSRSQDVLTIGATVAFSSFWLLPRLAEFRRANPGILVRVISQDSPIALDDGEVDVAVRYGLPPFSDSTVI
ASHGDVICPVCSPDYLRRRGDGALGSADEFIETDVLDRSWYSWAQWFSLTGANLEVKPSLRFNHYTETIAAARAGQGIAL
GWRMLVGTFLEDGTLVQVDGGELAAEDRYNVIVPVKAKRSNARDLAAAWLTASLHG

Sequences:

>Translated_296_residues
MMHFRRRIPSLTALVTLEAVLRKKSFTTAATELGVTQAAVSRQIALLEEEFGQPLFVRKHRAIEPTAACINLGATLAKSF
ADIAESVEALQSRSQDVLTIGATVAFSSFWLLPRLAEFRRANPGILVRVISQDSPIALDDGEVDVAVRYGLPPFSDSTVI
ASHGDVICPVCSPDYLRRRGDGALGSADEFIETDVLDRSWYSWAQWFSLTGANLEVKPSLRFNHYTETIAAARAGQGIAL
GWRMLVGTFLEDGTLVQVDGGELAAEDRYNVIVPVKAKRSNARDLAAAWLTASLHG
>Mature_296_residues
MMHFRRRIPSLTALVTLEAVLRKKSFTTAATELGVTQAAVSRQIALLEEEFGQPLFVRKHRAIEPTAACINLGATLAKSF
ADIAESVEALQSRSQDVLTIGATVAFSSFWLLPRLAEFRRANPGILVRVISQDSPIALDDGEVDVAVRYGLPPFSDSTVI
ASHGDVICPVCSPDYLRRRGDGALGSADEFIETDVLDRSWYSWAQWFSLTGANLEVKPSLRFNHYTETIAAARAGQGIAL
GWRMLVGTFLEDGTLVQVDGGELAAEDRYNVIVPVKAKRSNARDLAAAWLTASLHG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=256, Percent_Identity=33.984375, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788706, Length=258, Percent_Identity=29.8449612403101, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1786448, Length=287, Percent_Identity=26.8292682926829, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=26.027397260274, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789440, Length=260, Percent_Identity=24.2307692307692, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32244; Mature: 32244

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50931 HTH_LYSR ; PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMHFRRRIPSLTALVTLEAVLRKKSFTTAATELGVTQAAVSRQIALLEEEFGQPLFVRKH
CCCHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
RAIEPTAACINLGATLAKSFADIAESVEALQSRSQDVLTIGATVAFSSFWLLPRLAEFRR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHC
ANPGILVRVISQDSPIALDDGEVDVAVRYGLPPFSDSTVIASHGDVICPVCSPDYLRRRG
CCCCEEEEEECCCCCEEECCCCEEEEEEECCCCCCCCEEEECCCCEEEECCCHHHHHHCC
DGALGSADEFIETDVLDRSWYSWAQWFSLTGANLEVKPSLRFNHYTETIAAARAGQGIAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCHHH
GWRMLVGTFLEDGTLVQVDGGELAAEDRYNVIVPVKAKRSNARDLAAAWLTASLHG
HHHHHHHHHHCCCEEEEECCCCEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMHFRRRIPSLTALVTLEAVLRKKSFTTAATELGVTQAAVSRQIALLEEEFGQPLFVRKH
CCCHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
RAIEPTAACINLGATLAKSFADIAESVEALQSRSQDVLTIGATVAFSSFWLLPRLAEFRR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHC
ANPGILVRVISQDSPIALDDGEVDVAVRYGLPPFSDSTVIASHGDVICPVCSPDYLRRRG
CCCCEEEEEECCCCCEEECCCCEEEEEEECCCCCCCCEEEECCCCEEEECCCHHHHHHCC
DGALGSADEFIETDVLDRSWYSWAQWFSLTGANLEVKPSLRFNHYTETIAAARAGQGIAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCHHH
GWRMLVGTFLEDGTLVQVDGGELAAEDRYNVIVPVKAKRSNARDLAAAWLTASLHG
HHHHHHHHHHCCCEEEEECCCCEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]