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The map label for this gene is opuAB [H]

Identifier: 116249197

GI number: 116249197

Start: 578738

End: 579586

Strand: Direct

Name: opuAB [H]

Synonym: pRL120533

Alternate gene names: 116249197

Gene position: 578738-579586 (Clockwise)

Preceding gene: 116249196

Following gene: 116249198

Centisome position: 66.52

GC content: 61.37

Gene sequence:

>849_bases
ATGGATAGTTCAGGCTTCACCGATCTTTTTGATGAATGGACGGACTCGGCGCTCGAGTGGGTGAGCGACAACGGCGAATT
CCTTTTCGATTCCATCAGGCAGCTTCTCGAAGGGCTCTATGACGGGATCCTCTGGCTCCTGCAGCTTCCGCCTTTCTACC
TGGTCGCGCTGATCGTGGCGCTCATCGGCTGGCGGCTGGTCAATGTCTGGTTCGCTCTGCTGAGCGGCGCAGCACTGGCG
CTTTGTTTTTCCATGGGGCTCTGGCCGGAGACGATGAGCACCCTGGCGCTGGTATTGACCGCAACCGCACTCGCCCTGGC
GATCGGGATCCCGATCGGGATTGCCGCCGGTTTCTTGCCCGCCCTCGATCGGTTCATGGAGCCCGGCCTGGATCTTATCC
AGACGCTTCCGCCATACATCTACCTGCTGCCAGCGATCGCCCTGCTCGGCTACGGACCGGCGACGGCACTGATCGCGACG
GTGATCGTCGCCGTGCCGCCGGCGATCCGCCTGACCTCTCTCGGTATCCGGATGACGCCCAAGGAGTTCATCGAACTCGG
AGAGGCGCTCGGGATGACGCCGGCGAAGATGTTCTTCAAGATCCGCCTTCCCTTTGCGCTGCCGAGCATCATGGCAGGCA
TAAACCAGAGCCTGATGATGGCCTTCGGCATGGTCGTCATCGCCGGCATCGTCGGCTCCGGCGGGCTCGGCGAGACGATC
TATGGCGCGATCAGAACGCTCGACATCGCGACCTCTATCAATGGAGCGATCGCCATCGTGGTACTGACGATGGTGCTTGA
CCGGATAACCCAGAGTGCAGCTCGCTTGGGAACGGGGAGGAAGTCATGA

Upstream 100 bases:

>100_bases
AGATCGTCGGCGTGGTGACACCACGCAGCCTGCTGATGGGCGTCAAGGGAACCTCCACCCACGATCTCACGGCGGCGTCC
CACAACTGGAGCTGATTGAC

Downstream 100 bases:

>100_bases
ACCCATCCATCCTGCAGTTTTCGCCCGGCGCTTTCCTGGCGCCGTCAGTCGATTGGCTCAACACCAACCTGCATCCGCTG
TTTGCGGCGATCAGCTATCT

Product: putative permease component of ABC transporter

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MDSSGFTDLFDEWTDSALEWVSDNGEFLFDSIRQLLEGLYDGILWLLQLPPFYLVALIVALIGWRLVNVWFALLSGAALA
LCFSMGLWPETMSTLALVLTATALALAIGIPIGIAAGFLPALDRFMEPGLDLIQTLPPYIYLLPAIALLGYGPATALIAT
VIVAVPPAIRLTSLGIRMTPKEFIELGEALGMTPAKMFFKIRLPFALPSIMAGINQSLMMAFGMVVIAGIVGSGGLGETI
YGAIRTLDIATSINGAIAIVVLTMVLDRITQSAARLGTGRKS

Sequences:

>Translated_282_residues
MDSSGFTDLFDEWTDSALEWVSDNGEFLFDSIRQLLEGLYDGILWLLQLPPFYLVALIVALIGWRLVNVWFALLSGAALA
LCFSMGLWPETMSTLALVLTATALALAIGIPIGIAAGFLPALDRFMEPGLDLIQTLPPYIYLLPAIALLGYGPATALIAT
VIVAVPPAIRLTSLGIRMTPKEFIELGEALGMTPAKMFFKIRLPFALPSIMAGINQSLMMAFGMVVIAGIVGSGGLGETI
YGAIRTLDIATSINGAIAIVVLTMVLDRITQSAARLGTGRKS
>Mature_282_residues
MDSSGFTDLFDEWTDSALEWVSDNGEFLFDSIRQLLEGLYDGILWLLQLPPFYLVALIVALIGWRLVNVWFALLSGAALA
LCFSMGLWPETMSTLALVLTATALALAIGIPIGIAAGFLPALDRFMEPGLDLIQTLPPYIYLLPAIALLGYGPATALIAT
VIVAVPPAIRLTSLGIRMTPKEFIELGEALGMTPAKMFFKIRLPFALPSIMAGINQSLMMAFGMVVIAGIVGSGGLGETI
YGAIRTLDIATSINGAIAIVVLTMVLDRITQSAARLGTGRKS

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=273, Percent_Identity=36.996336996337, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1788451, Length=191, Percent_Identity=30.3664921465969, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788449, Length=239, Percent_Identity=29.7071129707113, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30079; Mature: 30079

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSSGFTDLFDEWTDSALEWVSDNGEFLFDSIRQLLEGLYDGILWLLQLPPFYLVALIVA
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LIGWRLVNVWFALLSGAALALCFSMGLWPETMSTLALVLTATALALAIGIPIGIAAGFLP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALDRFMEPGLDLIQTLPPYIYLLPAIALLGYGPATALIATVIVAVPPAIRLTSLGIRMTP
HHHHHHCCCHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCH
KEFIELGEALGMTPAKMFFKIRLPFALPSIMAGINQSLMMAFGMVVIAGIVGSGGLGETI
HHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
YGAIRTLDIATSINGAIAIVVLTMVLDRITQSAARLGTGRKS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDSSGFTDLFDEWTDSALEWVSDNGEFLFDSIRQLLEGLYDGILWLLQLPPFYLVALIVA
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LIGWRLVNVWFALLSGAALALCFSMGLWPETMSTLALVLTATALALAIGIPIGIAAGFLP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALDRFMEPGLDLIQTLPPYIYLLPAIALLGYGPATALIATVIVAVPPAIRLTSLGIRMTP
HHHHHHCCCHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCH
KEFIELGEALGMTPAKMFFKIRLPFALPSIMAGINQSLMMAFGMVVIAGIVGSGGLGETI
HHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
YGAIRTLDIATSINGAIAIVVLTMVLDRITQSAARLGTGRKS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]