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The map label for this gene is opuAB [H]

Identifier: 116249198

GI number: 116249198

Start: 579583

End: 580434

Strand: Direct

Name: opuAB [H]

Synonym: pRL120534

Alternate gene names: 116249198

Gene position: 579583-580434 (Clockwise)

Preceding gene: 116249197

Following gene: 116249199

Centisome position: 66.62

GC content: 63.85

Gene sequence:

>852_bases
ATGAACCCATCCATCCTGCAGTTTTCGCCCGGCGCTTTCCTGGCGCCGTCAGTCGATTGGCTCAACACCAACCTGCATCC
GCTGTTTGCGGCGATCAGCTATCTGATCGAAGCAGTCCTTTCCGGATTCGAGGCGCTCCTCCTCTTTGTGCCGCCCTATG
GGCTTATCGCGATGGTCGTCGTCCTGGCATTCGTCGCCGTCGGTCTGCGCGCCGCGATCCTTGCGGGGCTTTGCCTGGGG
TTCTGTCTCCTCATGGGGCTGTGGACGGCATCGATGCAGACCCTCTCCCTGGTCACTGTCGCGGTTCTGATCTCCGTGCT
CATCGCCTTCCCGATCGGCGTCCTGTGCTCGCGCCACAAGACATTGGAGGCAATGGTTCGCCCGGTTCTCGATGTGATGC
AGACCGTGCCGCCGTGGGTCTATCTCATTCCTGCGGTGATGATCTTCAGCCTTGGACGCGTGCCGGCAATCATCGCCACC
ATCGTTTATGGTATTCCGCCGATGCTCCGCCTGACCACGCTGGCGTTCAACCAGGTGCCGAGAGAATTCGTGGAACTCGG
CAGCGCCATCGGCGCGCCGCCTCGTTCGGTGCTTTGGAAGATCGAGATCCCCTTGGCCAGGCAGACGCTCCTGGTTGGGC
TCAACCAGTGCATCCTTCTGTCGCTGGCGATGGTCGTATTGGCCGGGCTCGTCGGCGCCGGCGGTCTCGGCGCGGAGGTG
ACGCGCGGCCTGACGCGCATGGAGATGGGGCTTGGCCTGCGGGCGGGCCTGGCGATCGTTGCAGTCGCCCTCCTGCTCGA
CCGACTGTCCCGCGGCCTGCTCGACCGCGACAGGCCACGGAAGGCAGGATGA

Upstream 100 bases:

>100_bases
TCGCGACCTCTATCAATGGAGCGATCGCCATCGTGGTACTGACGATGGTGCTTGACCGGATAACCCAGAGTGCAGCTCGC
TTGGGAACGGGGAGGAAGTC

Downstream 100 bases:

>100_bases
GCAAGGACATGAAAAGATGAGAAACAGCTTCTTCTGTATCGATGCGCATACCTGCGGCAATCCCGTCCGGCTCGTCGCCG
GAGGCGGTCCGCTGCTTCTG

Product: putative permease component of ABC transporter

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MNPSILQFSPGAFLAPSVDWLNTNLHPLFAAISYLIEAVLSGFEALLLFVPPYGLIAMVVVLAFVAVGLRAAILAGLCLG
FCLLMGLWTASMQTLSLVTVAVLISVLIAFPIGVLCSRHKTLEAMVRPVLDVMQTVPPWVYLIPAVMIFSLGRVPAIIAT
IVYGIPPMLRLTTLAFNQVPREFVELGSAIGAPPRSVLWKIEIPLARQTLLVGLNQCILLSLAMVVLAGLVGAGGLGAEV
TRGLTRMEMGLGLRAGLAIVAVALLLDRLSRGLLDRDRPRKAG

Sequences:

>Translated_283_residues
MNPSILQFSPGAFLAPSVDWLNTNLHPLFAAISYLIEAVLSGFEALLLFVPPYGLIAMVVVLAFVAVGLRAAILAGLCLG
FCLLMGLWTASMQTLSLVTVAVLISVLIAFPIGVLCSRHKTLEAMVRPVLDVMQTVPPWVYLIPAVMIFSLGRVPAIIAT
IVYGIPPMLRLTTLAFNQVPREFVELGSAIGAPPRSVLWKIEIPLARQTLLVGLNQCILLSLAMVVLAGLVGAGGLGAEV
TRGLTRMEMGLGLRAGLAIVAVALLLDRLSRGLLDRDRPRKAG
>Mature_283_residues
MNPSILQFSPGAFLAPSVDWLNTNLHPLFAAISYLIEAVLSGFEALLLFVPPYGLIAMVVVLAFVAVGLRAAILAGLCLG
FCLLMGLWTASMQTLSLVTVAVLISVLIAFPIGVLCSRHKTLEAMVRPVLDVMQTVPPWVYLIPAVMIFSLGRVPAIIAT
IVYGIPPMLRLTTLAFNQVPREFVELGSAIGAPPRSVLWKIEIPLARQTLLVGLNQCILLSLAMVVLAGLVGAGGLGAEV
TRGLTRMEMGLGLRAGLAIVAVALLLDRLSRGLLDRDRPRKAG

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=277, Percent_Identity=36.8231046931408, Blast_Score=188, Evalue=3e-49,
Organism=Escherichia coli, GI1788451, Length=196, Percent_Identity=29.5918367346939, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788449, Length=181, Percent_Identity=28.1767955801105, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30138; Mature: 30138

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPSILQFSPGAFLAPSVDWLNTNLHPLFAAISYLIEAVLSGFEALLLFVPPYGLIAMVV
CCCCCEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VLAFVAVGLRAAILAGLCLGFCLLMGLWTASMQTLSLVTVAVLISVLIAFPIGVLCSRHK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLEAMVRPVLDVMQTVPPWVYLIPAVMIFSLGRVPAIIATIVYGIPPMLRLTTLAFNQVP
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
REFVELGSAIGAPPRSVLWKIEIPLARQTLLVGLNQCILLSLAMVVLAGLVGAGGLGAEV
HHHHHHHHHCCCCHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
TRGLTRMEMGLGLRAGLAIVAVALLLDRLSRGLLDRDRPRKAG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNPSILQFSPGAFLAPSVDWLNTNLHPLFAAISYLIEAVLSGFEALLLFVPPYGLIAMVV
CCCCCEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VLAFVAVGLRAAILAGLCLGFCLLMGLWTASMQTLSLVTVAVLISVLIAFPIGVLCSRHK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLEAMVRPVLDVMQTVPPWVYLIPAVMIFSLGRVPAIIATIVYGIPPMLRLTTLAFNQVP
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
REFVELGSAIGAPPRSVLWKIEIPLARQTLLVGLNQCILLSLAMVVLAGLVGAGGLGAEV
HHHHHHHHHCCCCHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
TRGLTRMEMGLGLRAGLAIVAVALLLDRLSRGLLDRDRPRKAG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]