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The map label for this gene is stcD [H]

Identifier: 116249183

GI number: 116249183

Start: 563849

End: 565885

Strand: Direct

Name: stcD [H]

Synonym: pRL120519

Alternate gene names: 116249183

Gene position: 563849-565885 (Clockwise)

Preceding gene: 116249174

Following gene: 116249184

Centisome position: 64.81

GC content: 61.86

Gene sequence:

>2037_bases
ATGTCGAACGATCCCCTTCTTCAGCCCTATCAACTCAAGCATTTGACGCTACGCAACCGTATCATCGTGACCTCCCACGA
ACCGGCCTATCCGGAAGACGGCATGCCGAAGGAGAGGTATCGCGCCTATACAGTGGAGCGGGCAAAGGGCGGTGTGGCCT
TGGCGATGACGGCAGGCTCGGCCGCGGTTTCCAGGGACAGCCCGCCGGTCTTCAACAACCTGCTCGCCTACAAGGACGAA
ATCGTGCCCTGGATCAGGGAAATGACCGACGCCGTGCATGAAGAGGGTGCGGCGATCATGATCCAGCTCACCCATCTCGG
CCGGCGCACGCGCTGGGACAAGGGCGACTGGCTGCCGGTGGTGGCGCCGTCGCATCATCGCGAGGCTTCCCACCGCTCCT
TCCCGAAGAAGATGGAAGATTGGGACATCGAGCGCATCATCAAGGATTTCGCCGACGCTGCCGAGCGCATGAAGGCGGGC
GGCATGGACGGTGTCGAACTGGAAGCCTATGGCCACCTGATCGACCAGTTCACGTCGCCGCTCACCAACGAGCTCGACGG
TCCCTATGGCGGCTCGCTCGATAACCGTTTGCGCTTCTGCCTCGATGTGTTCAAAGCGATCCGGCAAAGGGTGGGGAACG
ACTTCATTCTGGGCGTGCGCTACACCGCCGACGAATGTCTTCCCGGCGGCACCGGCAAGGGCGACGGCATCGAAATCTCC
AAGCGCCTGAAAGAAAGCGGCCTGATCGACTACCTCAATGTGATCCGCGGCCACATCGATACCGATGCGGGTCTCACCGA
CGTGATCCCGATCCAGGGCATGGCGAGTTCACCGCATCTCGATTTCGCCGGCGAAATTCGCGCCGCAACCGATTTCCCGA
CCTTCCATGCCGCGAAAATCCCTGACGTCGCTACCGCGCGCCACGCGATCGCCTCCGGCAAGGTCGACATGGTCGGCATG
ACCCGCGCCCACATGACCGACCCCCACATCGTCCGCAAGATCATCGAAAAGCGCGAAGACGATATTCGACCCTGCGTCGG
CGCCAATTACTGTCTCGATCGCATTTACCAGGGTGGGGCCGCCTTCTGTATCCACAATGCCGCCACCGGCCGCGAACTGA
CCATGCCGCATATCGTGGCGAAGGCTGAGGCCAAGAAAAAGGTCGTCATCGTCGGCGCCGGCCCGGCCGGTCTGGAAGCG
GCGCGCGTCGCCGGAGAACGCGGCCACCAGGTCGTCGTTTTCGAAGCGGCGAACAATCCCGGCGGCCAGATCCGCCTCAC
CGCCCAGAGCGAGCGCCGCAGGGAGATGATCAGCATCATCGACTGGCGCATGAGCCAGTGCGAGAAATACGACGTCACCT
TCCACTTCAACACCTGGGCGGAGGCCGATACCATCGCGGCCGAAAATCCCGATGTCGTCATCATCGCGACCGGCGGCTTG
CCGCATACCGAGGTTCTCGCCAGCGGCAACGAGCTGGTGGTCTCGTCATGGGACATCATCTCCGGCGACGTGAAGCCTGG
AACCAATGTGCTGATCTTCGACGATGCCGGCGACCATGCCGGCCTTCAGGCGGCCGAGTTTCTCGCCAAGGCAGGCGCCA
AGGTCGAGATCATGACGCCGGACCGGTCCTTCGCGCCCGAGGTCATGGCCATGAACCTGGTGCCCTATATGCGGTCTCTG
CAGAAGCATGACGTGACCTTCACCGTCACCTACCGTCTCGAAGCCGTCGAGAAGAGCGGCAACCAGCTCGTCGCCCATAT
CGGCAGCGATTACGGTGGGATCGCCAGGCAGCACAGCTTCGACCAGATCGTCGTCAATCATGGGACCATTCCGCTCGATG
ATCTCTATTTCGAGCTGAAGCCCAGCTCGAGCAATCTCGGCGAGATGTCGCACGACCAGCTTCTTTCCGGAGAACCCCAG
TCCGTCATTCGCAATCCTGATGGCAAGTTCCAGCTGTTCCGGATCGGGGATGCCGTCGCCGCACGCAACACGCATGCCGC
CGTCTATGACGGACTGCGCATCGCGAAAGATATCTGA

Upstream 100 bases:

>100_bases
TTGGTGTCGAAAACGGCGACCCATATGTTTTCAATATTGACACATATGTACATTGTGCTTAGCGTCTGCCCTGGCGTTTT
TTAATCCGGAACCACAGCCC

Downstream 100 bases:

>100_bases
TCGCAGGAGCGGACCGCTTGCCGGCCGATCGACTCGGGAGGTGATGACATGAGTTTACGCAATGAAAGCCTGCAGATTTT
CCTGGATGCGGCTTTCGTGG

Product: putative flavoprotein oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: Stachydrine utilization protein stcD [H]

Number of amino acids: Translated: 678; Mature: 677

Protein sequence:

>678_residues
MSNDPLLQPYQLKHLTLRNRIIVTSHEPAYPEDGMPKERYRAYTVERAKGGVALAMTAGSAAVSRDSPPVFNNLLAYKDE
IVPWIREMTDAVHEEGAAIMIQLTHLGRRTRWDKGDWLPVVAPSHHREASHRSFPKKMEDWDIERIIKDFADAAERMKAG
GMDGVELEAYGHLIDQFTSPLTNELDGPYGGSLDNRLRFCLDVFKAIRQRVGNDFILGVRYTADECLPGGTGKGDGIEIS
KRLKESGLIDYLNVIRGHIDTDAGLTDVIPIQGMASSPHLDFAGEIRAATDFPTFHAAKIPDVATARHAIASGKVDMVGM
TRAHMTDPHIVRKIIEKREDDIRPCVGANYCLDRIYQGGAAFCIHNAATGRELTMPHIVAKAEAKKKVVIVGAGPAGLEA
ARVAGERGHQVVVFEAANNPGGQIRLTAQSERRREMISIIDWRMSQCEKYDVTFHFNTWAEADTIAAENPDVVIIATGGL
PHTEVLASGNELVVSSWDIISGDVKPGTNVLIFDDAGDHAGLQAAEFLAKAGAKVEIMTPDRSFAPEVMAMNLVPYMRSL
QKHDVTFTVTYRLEAVEKSGNQLVAHIGSDYGGIARQHSFDQIVVNHGTIPLDDLYFELKPSSSNLGEMSHDQLLSGEPQ
SVIRNPDGKFQLFRIGDAVAARNTHAAVYDGLRIAKDI

Sequences:

>Translated_678_residues
MSNDPLLQPYQLKHLTLRNRIIVTSHEPAYPEDGMPKERYRAYTVERAKGGVALAMTAGSAAVSRDSPPVFNNLLAYKDE
IVPWIREMTDAVHEEGAAIMIQLTHLGRRTRWDKGDWLPVVAPSHHREASHRSFPKKMEDWDIERIIKDFADAAERMKAG
GMDGVELEAYGHLIDQFTSPLTNELDGPYGGSLDNRLRFCLDVFKAIRQRVGNDFILGVRYTADECLPGGTGKGDGIEIS
KRLKESGLIDYLNVIRGHIDTDAGLTDVIPIQGMASSPHLDFAGEIRAATDFPTFHAAKIPDVATARHAIASGKVDMVGM
TRAHMTDPHIVRKIIEKREDDIRPCVGANYCLDRIYQGGAAFCIHNAATGRELTMPHIVAKAEAKKKVVIVGAGPAGLEA
ARVAGERGHQVVVFEAANNPGGQIRLTAQSERRREMISIIDWRMSQCEKYDVTFHFNTWAEADTIAAENPDVVIIATGGL
PHTEVLASGNELVVSSWDIISGDVKPGTNVLIFDDAGDHAGLQAAEFLAKAGAKVEIMTPDRSFAPEVMAMNLVPYMRSL
QKHDVTFTVTYRLEAVEKSGNQLVAHIGSDYGGIARQHSFDQIVVNHGTIPLDDLYFELKPSSSNLGEMSHDQLLSGEPQ
SVIRNPDGKFQLFRIGDAVAARNTHAAVYDGLRIAKDI
>Mature_677_residues
SNDPLLQPYQLKHLTLRNRIIVTSHEPAYPEDGMPKERYRAYTVERAKGGVALAMTAGSAAVSRDSPPVFNNLLAYKDEI
VPWIREMTDAVHEEGAAIMIQLTHLGRRTRWDKGDWLPVVAPSHHREASHRSFPKKMEDWDIERIIKDFADAAERMKAGG
MDGVELEAYGHLIDQFTSPLTNELDGPYGGSLDNRLRFCLDVFKAIRQRVGNDFILGVRYTADECLPGGTGKGDGIEISK
RLKESGLIDYLNVIRGHIDTDAGLTDVIPIQGMASSPHLDFAGEIRAATDFPTFHAAKIPDVATARHAIASGKVDMVGMT
RAHMTDPHIVRKIIEKREDDIRPCVGANYCLDRIYQGGAAFCIHNAATGRELTMPHIVAKAEAKKKVVIVGAGPAGLEAA
RVAGERGHQVVVFEAANNPGGQIRLTAQSERRREMISIIDWRMSQCEKYDVTFHFNTWAEADTIAAENPDVVIIATGGLP
HTEVLASGNELVVSSWDIISGDVKPGTNVLIFDDAGDHAGLQAAEFLAKAGAKVEIMTPDRSFAPEVMAMNLVPYMRSLQ
KHDVTFTVTYRLEAVEKSGNQLVAHIGSDYGGIARQHSFDQIVVNHGTIPLDDLYFELKPSSSNLGEMSHDQLLSGEPQS
VIRNPDGKFQLFRIGDAVAARNTHAAVYDGLRIAKDI

Specific function: Possible NADH-dependent oxidase, functions as a demethylase that converts N-methylproline to proline [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789463, Length=540, Percent_Identity=30.3703703703704, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI1787939, Length=388, Percent_Identity=26.5463917525773, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17565138, Length=307, Percent_Identity=27.3615635179153, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17559802, Length=276, Percent_Identity=28.2608695652174, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI72001454, Length=262, Percent_Identity=27.4809160305344, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17559804, Length=264, Percent_Identity=26.8939393939394, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17564188, Length=264, Percent_Identity=25.3787878787879, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17566914, Length=235, Percent_Identity=24.2553191489362, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=356, Percent_Identity=24.438202247191, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 74445; Mature: 74314

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDPLLQPYQLKHLTLRNRIIVTSHEPAYPEDGMPKERYRAYTVERAKGGVALAMTAGS
CCCCCCCCCHHHHEEEEECEEEEECCCCCCCCCCCCHHHHHEEEEEECCCCEEEEEECCC
AAVSRDSPPVFNNLLAYKDEIVPWIREMTDAVHEEGAAIMIQLTHLGRRTRWDKGDWLPV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEE
VAPSHHREASHRSFPKKMEDWDIERIIKDFADAAERMKAGGMDGVELEAYGHLIDQFTSP
ECCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCH
LTNELDGPYGGSLDNRLRFCLDVFKAIRQRVGNDFILGVRYTADECLPGGTGKGDGIEIS
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHCCCCCCCCCCCCHHH
KRLKESGLIDYLNVIRGHIDTDAGLTDVIPIQGMASSPHLDFAGEIRAATDFPTFHAAKI
HHHHHCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCC
PDVATARHAIASGKVDMVGMTRAHMTDPHIVRKIIEKREDDIRPCVGANYCLDRIYQGGA
CCHHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCC
AFCIHNAATGRELTMPHIVAKAEAKKKVVIVGAGPAGLEAARVAGERGHQVVVFEAANNP
EEEEECCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCC
GGQIRLTAQSERRREMISIIDWRMSQCEKYDVTFHFNTWAEADTIAAENPDVVIIATGGL
CCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEECCCCCEEEEECCCC
PHTEVLASGNELVVSSWDIISGDVKPGTNVLIFDDAGDHAGLQAAEFLAKAGAKVEIMTP
CHHHHHHCCCEEEEEECCEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEECC
DRSFAPEVMAMNLVPYMRSLQKHDVTFTVTYRLEAVEKSGNQLVAHIGSDYGGIARQHSF
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEEECCCCCCHHHHCCC
DQIVVNHGTIPLDDLYFELKPSSSNLGEMSHDQLLSGEPQSVIRNPDGKFQLFRIGDAVA
CEEEEECCCCCHHHEEEEEECCCCCCCCCCHHHHCCCCCHHHHCCCCCCEEEEEECCHHH
ARNTHAAVYDGLRIAKDI
CCCCCHHHHCCCHHHCCC
>Mature Secondary Structure 
SNDPLLQPYQLKHLTLRNRIIVTSHEPAYPEDGMPKERYRAYTVERAKGGVALAMTAGS
CCCCCCCCHHHHEEEEECEEEEECCCCCCCCCCCCHHHHHEEEEEECCCCEEEEEECCC
AAVSRDSPPVFNNLLAYKDEIVPWIREMTDAVHEEGAAIMIQLTHLGRRTRWDKGDWLPV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEE
VAPSHHREASHRSFPKKMEDWDIERIIKDFADAAERMKAGGMDGVELEAYGHLIDQFTSP
ECCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCH
LTNELDGPYGGSLDNRLRFCLDVFKAIRQRVGNDFILGVRYTADECLPGGTGKGDGIEIS
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHCCCCCCCCCCCCHHH
KRLKESGLIDYLNVIRGHIDTDAGLTDVIPIQGMASSPHLDFAGEIRAATDFPTFHAAKI
HHHHHCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCC
PDVATARHAIASGKVDMVGMTRAHMTDPHIVRKIIEKREDDIRPCVGANYCLDRIYQGGA
CCHHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCC
AFCIHNAATGRELTMPHIVAKAEAKKKVVIVGAGPAGLEAARVAGERGHQVVVFEAANNP
EEEEECCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCC
GGQIRLTAQSERRREMISIIDWRMSQCEKYDVTFHFNTWAEADTIAAENPDVVIIATGGL
CCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEECCCCCEEEEECCCC
PHTEVLASGNELVVSSWDIISGDVKPGTNVLIFDDAGDHAGLQAAEFLAKAGAKVEIMTP
CHHHHHHCCCEEEEEECCEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEECC
DRSFAPEVMAMNLVPYMRSLQKHDVTFTVTYRLEAVEKSGNQLVAHIGSDYGGIARQHSF
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEEECCCCCCHHHHCCC
DQIVVNHGTIPLDDLYFELKPSSSNLGEMSHDQLLSGEPQSVIRNPDGKFQLFRIGDAVA
CEEEEECCCCCHHHEEEEEECCCCCCCCCCHHHHCCCCCHHHHCCCCCCEEEEEECCHHH
ARNTHAAVYDGLRIAKDI
CCCCCHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9758825; 11481431 [H]