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The map label for this gene is ech [C]

Identifier: 116249169

GI number: 116249169

Start: 547692

End: 548516

Strand: Direct

Name: ech [C]

Synonym: pRL120505

Alternate gene names: 116249169

Gene position: 547692-548516 (Clockwise)

Preceding gene: 116249168

Following gene: 116249170

Centisome position: 62.95

GC content: 62.42

Gene sequence:

>825_bases
ATGACTGAAGACAAATCGCCGGTTCTGGTCGAATTCGACAGCGGCATCGCCTTCGTGACCCTCAACCGTCCGGAAAAGCG
CAATGCGATGAATCCGGCGCTGAATGCCCGAATGCTCGAAGTGCTTGACGAGCTCGAGGGCGACGAGCGTTGCGGCGTCC
TCGTCCTGCGCGGCGCCGGCGAATCCTGGTCGGCCGGCATGGATCTGAAGGAATATTTCCGCGACAATGACGACAAGCCG
CGTGACGTCACGCTGAAGGCGCGGCGCCAGTCCGGCAACTGGTGGGGCCGGCTGATGTATTTCGAAAAGCCGACGATCGC
CATGGTCAACGGCTGGTGCTTCGGCGGCGCATTCACACCGCTGGTTTCCTGCGATCTCGCCATCGCCGCCGAGGAGGCAA
ATTTCGGCCTCTCCGAGATCAACTGGGGCATTCTGCCGGGCGGCAATGTCACCCGCGCGGTCGCCGAAGTGATGCGCCAT
CGCGACGCCCTCTATTACATCATGACCGGCGAACTGTTCGGCGGACGCAAGGCCGCCGAAATGGGTCTCGTCAACGAGGC
GGTGCCGCTGGCGGAGCTGGAAATCCGGGTCCGCAAGATCTGCGCCAGCCTTCTCGAAAAGAACCCGGTGACGCTGAAGG
CCGCCAAGGACACCTACAAGCGGGTGCGCAACCTGCCATGGGATCTCGCCGACGATTACATCTACGCCAAGCTGGAGCAG
ATGCTGTTTCTCGACAAGACCAAGGGGCGCGACGAGGGGCTGAAACAGTTCCTCGACGACAAAACCTATCAGCCGGGGCT
CGGCGCCTACCAGCGCGACCGCTGA

Upstream 100 bases:

>100_bases
GAAACATGTCCTGCGCCAGTTCCATTCCGACGAAACCCTTCAACGACAGGCCCTGTGAAGCGGCCCCCCGGCATCACGCC
GAAACCCATGGAGTAACCTC

Downstream 100 bases:

>100_bases
GCACGGGTCCGGTTCGCCGGGCCGTTCACGAGCCGATCATCGGGAGGAGTAAAGCAATGAAAATCCATGCCGCGGTGGCG
CGTGCGCCCCATACGCCGTT

Product: p-hydroxycinnamoyl CoA hydratase/lyase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTEDKSPVLVEFDSGIAFVTLNRPEKRNAMNPALNARMLEVLDELEGDERCGVLVLRGAGESWSAGMDLKEYFRDNDDKP
RDVTLKARRQSGNWWGRLMYFEKPTIAMVNGWCFGGAFTPLVSCDLAIAAEEANFGLSEINWGILPGGNVTRAVAEVMRH
RDALYYIMTGELFGGRKAAEMGLVNEAVPLAELEIRVRKICASLLEKNPVTLKAAKDTYKRVRNLPWDLADDYIYAKLEQ
MLFLDKTKGRDEGLKQFLDDKTYQPGLGAYQRDR

Sequences:

>Translated_274_residues
MTEDKSPVLVEFDSGIAFVTLNRPEKRNAMNPALNARMLEVLDELEGDERCGVLVLRGAGESWSAGMDLKEYFRDNDDKP
RDVTLKARRQSGNWWGRLMYFEKPTIAMVNGWCFGGAFTPLVSCDLAIAAEEANFGLSEINWGILPGGNVTRAVAEVMRH
RDALYYIMTGELFGGRKAAEMGLVNEAVPLAELEIRVRKICASLLEKNPVTLKAAKDTYKRVRNLPWDLADDYIYAKLEQ
MLFLDKTKGRDEGLKQFLDDKTYQPGLGAYQRDR
>Mature_273_residues
TEDKSPVLVEFDSGIAFVTLNRPEKRNAMNPALNARMLEVLDELEGDERCGVLVLRGAGESWSAGMDLKEYFRDNDDKPR
DVTLKARRQSGNWWGRLMYFEKPTIAMVNGWCFGGAFTPLVSCDLAIAAEEANFGLSEINWGILPGGNVTRAVAEVMRHR
DALYYIMTGELFGGRKAAEMGLVNEAVPLAELEIRVRKICASLLEKNPVTLKAAKDTYKRVRNLPWDLADDYIYAKLEQM
LFLDKTKGRDEGLKQFLDDKTYQPGLGAYQRDR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=253, Percent_Identity=25.6916996047431, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI31542718, Length=256, Percent_Identity=28.90625, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI70995211, Length=216, Percent_Identity=28.7037037037037, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI45643119, Length=217, Percent_Identity=26.2672811059908, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI260275230, Length=217, Percent_Identity=26.2672811059908, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI260274832, Length=217, Percent_Identity=26.2672811059908, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI213417737, Length=176, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI157694516, Length=176, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1787659, Length=268, Percent_Identity=29.8507462686567, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1788597, Length=208, Percent_Identity=32.2115384615385, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI221142681, Length=223, Percent_Identity=30.9417040358744, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI87082183, Length=276, Percent_Identity=25.7246376811594, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787660, Length=181, Percent_Identity=29.2817679558011, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790281, Length=184, Percent_Identity=26.6304347826087, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1788682, Length=178, Percent_Identity=25.2808988764045, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=271, Percent_Identity=26.5682656826568, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=205, Percent_Identity=28.780487804878, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=28.2352941176471, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=214, Percent_Identity=28.9719626168224, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=235, Percent_Identity=24.6808510638298, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=246, Percent_Identity=23.5772357723577, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=201, Percent_Identity=26.3681592039801, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=199, Percent_Identity=26.6331658291457, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=188, Percent_Identity=27.6595744680851, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=188, Percent_Identity=27.6595744680851, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=188, Percent_Identity=27.6595744680851, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=203, Percent_Identity=26.6009852216749, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=200, Percent_Identity=25, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI20129971, Length=181, Percent_Identity=29.2817679558011, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24653477, Length=181, Percent_Identity=29.2817679558011, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24653139, Length=272, Percent_Identity=28.3088235294118, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357171, Length=185, Percent_Identity=30.2702702702703, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=28.735632183908, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24650670, Length=182, Percent_Identity=30.7692307692308, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24583165, Length=269, Percent_Identity=25.6505576208178, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30912; Mature: 30781

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEDKSPVLVEFDSGIAFVTLNRPEKRNAMNPALNARMLEVLDELEGDERCGVLVLRGAG
CCCCCCCEEEEECCCEEEEEECCCHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCC
ESWSAGMDLKEYFRDNDDKPRDVTLKARRQSGNWWGRLMYFEKPTIAMVNGWCFGGAFTP
CCCCCCCCHHHHHCCCCCCCCCEEEEEECCCCCEEEEEEEEECCCEEEEECEEECCHHHH
LVSCDLAIAAEEANFGLSEINWGILPGGNVTRAVAEVMRHRDALYYIMTGELFGGRKAAE
HHHCCEEEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHH
MGLVNEAVPLAELEIRVRKICASLLEKNPVTLKAAKDTYKRVRNLPWDLADDYIYAKLEQ
HCCHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHH
MLFLDKTKGRDEGLKQFLDDKTYQPGLGAYQRDR
HHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEDKSPVLVEFDSGIAFVTLNRPEKRNAMNPALNARMLEVLDELEGDERCGVLVLRGAG
CCCCCCEEEEECCCEEEEEECCCHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCC
ESWSAGMDLKEYFRDNDDKPRDVTLKARRQSGNWWGRLMYFEKPTIAMVNGWCFGGAFTP
CCCCCCCCHHHHHCCCCCCCCCEEEEEECCCCCEEEEEEEEECCCEEEEECEEECCHHHH
LVSCDLAIAAEEANFGLSEINWGILPGGNVTRAVAEVMRHRDALYYIMTGELFGGRKAAE
HHHCCEEEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHH
MGLVNEAVPLAELEIRVRKICASLLEKNPVTLKAAKDTYKRVRNLPWDLADDYIYAKLEQ
HCCHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHH
MLFLDKTKGRDEGLKQFLDDKTYQPGLGAYQRDR
HHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA