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The map label for this gene is nfdA [H]

Identifier: 116249118

GI number: 116249118

Start: 488239

End: 490116

Strand: Reverse

Name: nfdA [H]

Synonym: pRL120453

Alternate gene names: 116249118

Gene position: 490116-488239 (Counterclockwise)

Preceding gene: 116249119

Following gene: 116249117

Centisome position: 56.33

GC content: 61.93

Gene sequence:

>1878_bases
ATGCACGCCGATATCGTTCTTCACAATGGGCGGATCACAACGCTTGACCGCGCAAATCCGAACGCCACCGCGATCGCCGT
GAAAGACGGCCTGTTCCTCGAGGTGGGAACCGACAGCGAGGTGATGGCGCTCGCCGGACTGGATACGAAGATCGTCGACC
TCAAGGGCAAGCGCGTGCTTCCCGGCCTGATCGACAACCACACGCATGTCGTGCGTGGCGGCCTCAACTTCAACATGGAA
CTCCGCTGGGACGGTGTTCGCTCGCTTGCCGATGCGATGGACATGTTGAAGCGACAGGTGGCGATCACGCCCGCGCCGCA
ATGGGTTCGCGTGGTCGGTGGTTTTACCGAGCATCAGTTTACCGAGAAGCGCCTGCCGACGATCGACGAGATCAACGCGA
TCGCACCCGATACCCCGGTCTTCCTGCTCCACCTCTATGACCGCGCGCTGCTGAACGGCGCCGCCCTTCGCGCCGTCGGC
TATACCAAAGACACGCCGAACCCTCCAGGCGGCGAGATCACCCGCGATGCCAACGGCAATCCGACGGGGCTTCTGCTTGC
CAAGCCGAATGCGGGCATTCTCTATTCGACCCTTTCCAAGGGGCCGAAGCTTCCGTTCGAATATCAGGTCAATTCCACCC
GCCACTTCATGCGCGAACTCAACCGGCTGGGCATCACCGGCGTGATCGACGCCGGCGGCGGTTTCCAGAACTATCCGGAC
GATTATGCGGTCATCCAGAAGCTTTCGGACGAGGACCAGTTGACGGTGCGCCTCGCCTACAACCTGTTCACGCAGAAGCC
GAAGGAAGAGAAGCAGGACTTCCTCAACTGGACGCAGTCGGTCAAATACAAGCAGGGCAACGATTACTTCCGGCATAACG
GCGCTGGGGAGATGCTCGTCTTCTCCGCCGCCGACTTCGAGGATTTCCGTCAACCACGGCCCGAAATGGCGCCGGAAATG
GAAGGAGAACTTGAGGAAGTTGTTCGCGTGCTTGCCGAGAACCGTTGGCCCTGGCGCATGCATGCCACCTATGACGAAAC
CATTTCCCGTTCGCTCGACGTCTTCGAGAAGGTCAACAAGGACATTCCGCTCGAAGGTTTGAACTGGTTCTTCGACCATG
CCGAAACGATCTCGGATCGTTCGATCGACCGGATCGCGGCGCTCGGCGGCGGCATCGCCACTCAGCACCGCATGGCCTAT
CAGGGCGAATATTTCGTCGAGCGCTACGGTCACGGCGTCGCCGAGGCCACGCCGCCGATCAGGCGCATGCTTGACAAGGG
CGTCAACGTATCGGCAGGGACTGACGCGACGCGTGTCGCCTCCTACAATCCATGGGTTTCCCTTGCCTGGATGGTGACCG
GCAAGACGGTGGGCGGCATGCAGCTCTACCCGCGCGCCAACTGCCTCGACCGCGAGACGGCGCTGCGCATGTGGACGGAA
AAGGTGACCTGGTTCTCCAACGAGGAAGGCAAGAAGGGCCGGATCGAAAAAGGCCAGTTTGCCGACGTCGTCGTGCCGGA
CAAGGACTTCTTCACCTGCGCCGAAGACGAGATCTCGTACCTCACCTCGCAGTTGACCATGGTCGGCGGCAAGATCGTCT
ATGGCGCGGGCGATTTTGCGGCGCTCGACGAGAATGACGTCCCGCCCGCCATGCCCGACTGGTCACCCGTTCGCACGTTC
GGCGGCTATGCGGCCTGGGGTGAACCGCAGGGTGCCGGCGTGAATTCCCTGCGCCGGACGGCAATCTCGACCTGCGGCTG
CGCCAGTTCCTGCAATGTCCACGGTCACGACCACGCTGGCGCCTGGACATCGAAGCTGCCGATCGCCGATCTCAAGGGCT
TCTTCGGCGCCCTTGGCTGCGCCTGCTGGGCGGTGTGA

Upstream 100 bases:

>100_bases
CACGCGCCGCACATTCCTGGGCGCAGCATCGAGCCTCGCCTTTTCAAACTTGTTTTCACCGGCCAATGCCGCCGATCCTA
TCAAGACCGGAGCCGCTTCC

Downstream 100 bases:

>100_bases
TTTTCAACATCATCCATGAGGCAGCAGCAGATCGCTGCCCCATGGAAGACTATTGCGAATATCGCAAGAGAATCTCAATT
TTTGATGCAATTGTTGGCTC

Product: putative transcriptional regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MHADIVLHNGRITTLDRANPNATAIAVKDGLFLEVGTDSEVMALAGLDTKIVDLKGKRVLPGLIDNHTHVVRGGLNFNME
LRWDGVRSLADAMDMLKRQVAITPAPQWVRVVGGFTEHQFTEKRLPTIDEINAIAPDTPVFLLHLYDRALLNGAALRAVG
YTKDTPNPPGGEITRDANGNPTGLLLAKPNAGILYSTLSKGPKLPFEYQVNSTRHFMRELNRLGITGVIDAGGGFQNYPD
DYAVIQKLSDEDQLTVRLAYNLFTQKPKEEKQDFLNWTQSVKYKQGNDYFRHNGAGEMLVFSAADFEDFRQPRPEMAPEM
EGELEEVVRVLAENRWPWRMHATYDETISRSLDVFEKVNKDIPLEGLNWFFDHAETISDRSIDRIAALGGGIATQHRMAY
QGEYFVERYGHGVAEATPPIRRMLDKGVNVSAGTDATRVASYNPWVSLAWMVTGKTVGGMQLYPRANCLDRETALRMWTE
KVTWFSNEEGKKGRIEKGQFADVVVPDKDFFTCAEDEISYLTSQLTMVGGKIVYGAGDFAALDENDVPPAMPDWSPVRTF
GGYAAWGEPQGAGVNSLRRTAISTCGCASSCNVHGHDHAGAWTSKLPIADLKGFFGALGCACWAV

Sequences:

>Translated_625_residues
MHADIVLHNGRITTLDRANPNATAIAVKDGLFLEVGTDSEVMALAGLDTKIVDLKGKRVLPGLIDNHTHVVRGGLNFNME
LRWDGVRSLADAMDMLKRQVAITPAPQWVRVVGGFTEHQFTEKRLPTIDEINAIAPDTPVFLLHLYDRALLNGAALRAVG
YTKDTPNPPGGEITRDANGNPTGLLLAKPNAGILYSTLSKGPKLPFEYQVNSTRHFMRELNRLGITGVIDAGGGFQNYPD
DYAVIQKLSDEDQLTVRLAYNLFTQKPKEEKQDFLNWTQSVKYKQGNDYFRHNGAGEMLVFSAADFEDFRQPRPEMAPEM
EGELEEVVRVLAENRWPWRMHATYDETISRSLDVFEKVNKDIPLEGLNWFFDHAETISDRSIDRIAALGGGIATQHRMAY
QGEYFVERYGHGVAEATPPIRRMLDKGVNVSAGTDATRVASYNPWVSLAWMVTGKTVGGMQLYPRANCLDRETALRMWTE
KVTWFSNEEGKKGRIEKGQFADVVVPDKDFFTCAEDEISYLTSQLTMVGGKIVYGAGDFAALDENDVPPAMPDWSPVRTF
GGYAAWGEPQGAGVNSLRRTAISTCGCASSCNVHGHDHAGAWTSKLPIADLKGFFGALGCACWAV
>Mature_625_residues
MHADIVLHNGRITTLDRANPNATAIAVKDGLFLEVGTDSEVMALAGLDTKIVDLKGKRVLPGLIDNHTHVVRGGLNFNME
LRWDGVRSLADAMDMLKRQVAITPAPQWVRVVGGFTEHQFTEKRLPTIDEINAIAPDTPVFLLHLYDRALLNGAALRAVG
YTKDTPNPPGGEITRDANGNPTGLLLAKPNAGILYSTLSKGPKLPFEYQVNSTRHFMRELNRLGITGVIDAGGGFQNYPD
DYAVIQKLSDEDQLTVRLAYNLFTQKPKEEKQDFLNWTQSVKYKQGNDYFRHNGAGEMLVFSAADFEDFRQPRPEMAPEM
EGELEEVVRVLAENRWPWRMHATYDETISRSLDVFEKVNKDIPLEGLNWFFDHAETISDRSIDRIAALGGGIATQHRMAY
QGEYFVERYGHGVAEATPPIRRMLDKGVNVSAGTDATRVASYNPWVSLAWMVTGKTVGGMQLYPRANCLDRETALRMWTE
KVTWFSNEEGKKGRIEKGQFADVVVPDKDFFTCAEDEISYLTSQLTMVGGKIVYGAGDFAALDENDVPPAMPDWSPVRTF
GGYAAWGEPQGAGVNSLRRTAISTCGCASSCNVHGHDHAGAWTSKLPIADLKGFFGALGCACWAV

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 69140; Mature: 69140

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHADIVLHNGRITTLDRANPNATAIAVKDGLFLEVGTDSEVMALAGLDTKIVDLKGKRVL
CCCEEEEECCEEEEEECCCCCEEEEEEECCEEEEECCCCCEEEEECCCEEEEECCCCEEC
PGLIDNHTHVVRGGLNFNMELRWDGVRSLADAMDMLKRQVAITPAPQWVRVVGGFTEHQF
CCHHCCCCEEEECCCEEEEEEEECHHHHHHHHHHHHHHHEECCCCCHHHHHHCCCHHHHH
TEKRLPTIDEINAIAPDTPVFLLHLYDRALLNGAALRAVGYTKDTPNPPGGEITRDANGN
HHHHCCCHHHHCCCCCCCCEEHHHHHHHHHHCCHHEEEECCCCCCCCCCCCCEEECCCCC
PTGLLLAKPNAGILYSTLSKGPKLPFEYQVNSTRHFMRELNRLGITGVIDAGGGFQNYPD
CCEEEEECCCCCEEEHHHCCCCCCCEEEECCHHHHHHHHHHHCCCEEEEECCCCCCCCCC
DYAVIQKLSDEDQLTVRLAYNLFTQKPKEEKQDFLNWTQSVKYKQGNDYFRHNGAGEMLV
HHHHHHHCCCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCCCEEE
FSAADFEDFRQPRPEMAPEMEGELEEVVRVLAENRWPWRMHATYDETISRSLDVFEKVNK
EECCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCCC
DIPLEGLNWFFDHAETISDRSIDRIAALGGGIATQHRMAYQGEYFVERYGHGVAEATPPI
CCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCCCCCCCHHH
RRMLDKGVNVSAGTDATRVASYNPWVSLAWMVTGKTVGGMQLYPRANCLDRETALRMWTE
HHHHHCCCCCCCCCCCCEEECCCCCEEEEEEEECCCCCCEEECCCCCCCCHHHHHHHHHH
KVTWFSNEEGKKGRIEKGQFADVVVPDKDFFTCAEDEISYLTSQLTMVGGKIVYGAGDFA
HHEEECCCCCCCCCCCCCCEEEEEECCCCCEEECHHHHHHHHHHHHHHCCEEEECCCCEE
ALDENDVPPAMPDWSPVRTFGGYAAWGEPQGAGVNSLRRTAISTCGCASSCNVHGHDHAG
ECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
AWTSKLPIADLKGFFGALGCACWAV
CCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHADIVLHNGRITTLDRANPNATAIAVKDGLFLEVGTDSEVMALAGLDTKIVDLKGKRVL
CCCEEEEECCEEEEEECCCCCEEEEEEECCEEEEECCCCCEEEEECCCEEEEECCCCEEC
PGLIDNHTHVVRGGLNFNMELRWDGVRSLADAMDMLKRQVAITPAPQWVRVVGGFTEHQF
CCHHCCCCEEEECCCEEEEEEEECHHHHHHHHHHHHHHHEECCCCCHHHHHHCCCHHHHH
TEKRLPTIDEINAIAPDTPVFLLHLYDRALLNGAALRAVGYTKDTPNPPGGEITRDANGN
HHHHCCCHHHHCCCCCCCCEEHHHHHHHHHHCCHHEEEECCCCCCCCCCCCCEEECCCCC
PTGLLLAKPNAGILYSTLSKGPKLPFEYQVNSTRHFMRELNRLGITGVIDAGGGFQNYPD
CCEEEEECCCCCEEEHHHCCCCCCCEEEECCHHHHHHHHHHHCCCEEEEECCCCCCCCCC
DYAVIQKLSDEDQLTVRLAYNLFTQKPKEEKQDFLNWTQSVKYKQGNDYFRHNGAGEMLV
HHHHHHHCCCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCCCEEE
FSAADFEDFRQPRPEMAPEMEGELEEVVRVLAENRWPWRMHATYDETISRSLDVFEKVNK
EECCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCCC
DIPLEGLNWFFDHAETISDRSIDRIAALGGGIATQHRMAYQGEYFVERYGHGVAEATPPI
CCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCCCCCCCHHH
RRMLDKGVNVSAGTDATRVASYNPWVSLAWMVTGKTVGGMQLYPRANCLDRETALRMWTE
HHHHHCCCCCCCCCCCCEEECCCCCEEEEEEEECCCCCCEEECCCCCCCCHHHHHHHHHH
KVTWFSNEEGKKGRIEKGQFADVVVPDKDFFTCAEDEISYLTSQLTMVGGKIVYGAGDFA
HHEEECCCCCCCCCCCCCCEEEEEECCCCCEEECHHHHHHHHHHHHHHCCEEEECCCCEE
ALDENDVPPAMPDWSPVRTFGGYAAWGEPQGAGVNSLRRTAISTCGCASSCNVHGHDHAG
ECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
AWTSKLPIADLKGFFGALGCACWAV
CCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA