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The map label for this gene is 116249105

Identifier: 116249105

GI number: 116249105

Start: 473656

End: 475407

Strand: Reverse

Name: 116249105

Synonym: pRL120440

Alternate gene names: NA

Gene position: 475407-473656 (Counterclockwise)

Preceding gene: 116249106

Following gene: 116249104

Centisome position: 54.64

GC content: 61.53

Gene sequence:

>1752_bases
ATGGGAACAACCATGACATCGACACAAAGCTATACCGCGGCCACCATCCAGTTCGAACCGACCATGTTCGAAAAGGCGCG
CAATATCAGCCGGCTGACAGCCCTTTGCGAGGAGGCAGCGGAGGCAGGTGCGCGCCTGATCGTCACGCCCGAAATGGGCA
CGACCGGCTATTGCTGGTTCGACAGGGCCGAGGTGAAACCTTTCGTCGAGACTATCCCCGGAGCGACCACGGATGTTTTC
CAGGCCATTGCCAGCAAACACCGCTGCTATATCGTCGTCGGCATGCCGGAAGTCGATCCGGCAAGCGATCTTTACTACAA
TACCGCCGTCCTGATCGGCCCAGGCGGCGTCGTTGGCCGGCATCGCAAATCGCATCCCTATATCGCGGAACCGAAATGGG
CTGCCAACGGCGACATCGTCCACGAGGTATTCGAAACCGAGATCGGCCGGATCTCGATGCTGGTCTGCATGGATCTGCAC
TTCTTCGAGACGGCGCGCCTGGAGGCACTCGCCGGTGCCGACATCATCTGCCATATCTCCAACTGGCTGCAGGAGCGCAC
GCCGGCACCCTATTGGATCAACCGCGCCTTCGAAAACGCCTGCTATGTGATCGAAAGCAACCGGTGGGGGCTGGAGAGAA
CCGTGCAGTTTTCCGGTGGAAGCTGCCTGATCGAACCGGACGGCACGGTCGCAGCATCGATCGATACGGGCGACGGGATT
GCCTATGGCACGGTCGATCTTGCCCGCGCCCGCCGCCGCGAGGTCTTGTTCGAACCGGTCTTCAAGTCCCGCCGGCCCGA
GCTCTACATGAACATGATGACCAACAGCTTCACCTGGAATCCGGGCGATTACTTCCGTCTCTATGGCTACCAGCCGATCC
CGCGCGGACGCGCATCGCGCGCCGCCGTCGCCCAGTTCGCCCCCACCTCCGTTGTCGCCGACAATCTCGCCCGCATCGCC
GATCTGGCAGCCGAGGCGAAGGCAACGACAGCACCGGATATTCTGGTGTTCCCGGAACTATCTCTGACAGGATTGGAGGC
CCCGCATGGCAGGGCGGAATCGCTCTCGGGCCCGACGGTATCGGCCTTCGTTCGTCTGGCGATGAAGCTCGGTTTCTATC
TTGTAGCCGGATTTGCCGAAGAGGACGGGGACAAGGTCTACAACAGCGCTGTTCTCGCCGGCCCCGAGGGCCTCGTGGGC
AGCTATCGCAAGACACATCTCGGCATTGCCGACAGCTGGGCGACGGCCGGCGACGAATGGAAAGTCTACGATCTTGCCAT
CGGCCGCGTCGGCCTGGCGGTCGGCCATGACGCGCTCTATCCGGAAGCCATCCGTTCGCTCGCGCTGATGGGATGCGACG
TGGTCGCTTGCCCATCAGCCATAGCAGGCACTTTCACCGGCAGCCACACCGGCACGAAAATCCCGCATAACTATCCGATC
CCCAAGGGCGCCGATCCCTACCACTGGCATGCGTTGCGCGTGCGCGGTGGCGAGAACAACCTCTATTTCGCCTTCGCCAA
CGTCCTGGATGCGGCACGGGGATACCAAGGCAAGAGCGCTGTCTTCGGGCCGGATTCCTTTGCCTTTCCGCGTCAGGAAT
CGGCAATCCTGGACGAGGACGGGATTGCGGCGGCAGCCGTCGACACCACCAATCTCGACACGCCTTATCCGACGAACATC
GTCAGACGAAAGGATCTTGTCGTCATGCGCCAGCCGCATCACTATCAGCCGCTCGTCAAATGGCATCAGTGA

Upstream 100 bases:

>100_bases
GGAGGCGTGGTCGCGGCCGGCGCCATCGACACGCTCTCCGACGCGCTTGTTCATCGGCATATGGCTGTCTGAAACAGACC
GATATCCTTTCATAGAGACA

Downstream 100 bases:

>100_bases
GAGACAGGCCAAGATGTCCACATTCACATTCGCGACCGTCCCGCAGATCATCGCGGGACCGGGCTGCATCGCAAGGCTTT
CGGAGCTGACGAAAACGCGA

Product: putative nitrilase/cyanide hydratase family protein

Products: NA

Alternate protein names: ORF 5 [H]

Number of amino acids: Translated: 583; Mature: 582

Protein sequence:

>583_residues
MGTTMTSTQSYTAATIQFEPTMFEKARNISRLTALCEEAAEAGARLIVTPEMGTTGYCWFDRAEVKPFVETIPGATTDVF
QAIASKHRCYIVVGMPEVDPASDLYYNTAVLIGPGGVVGRHRKSHPYIAEPKWAANGDIVHEVFETEIGRISMLVCMDLH
FFETARLEALAGADIICHISNWLQERTPAPYWINRAFENACYVIESNRWGLERTVQFSGGSCLIEPDGTVAASIDTGDGI
AYGTVDLARARRREVLFEPVFKSRRPELYMNMMTNSFTWNPGDYFRLYGYQPIPRGRASRAAVAQFAPTSVVADNLARIA
DLAAEAKATTAPDILVFPELSLTGLEAPHGRAESLSGPTVSAFVRLAMKLGFYLVAGFAEEDGDKVYNSAVLAGPEGLVG
SYRKTHLGIADSWATAGDEWKVYDLAIGRVGLAVGHDALYPEAIRSLALMGCDVVACPSAIAGTFTGSHTGTKIPHNYPI
PKGADPYHWHALRVRGGENNLYFAFANVLDAARGYQGKSAVFGPDSFAFPRQESAILDEDGIAAAAVDTTNLDTPYPTNI
VRRKDLVVMRQPHHYQPLVKWHQ

Sequences:

>Translated_583_residues
MGTTMTSTQSYTAATIQFEPTMFEKARNISRLTALCEEAAEAGARLIVTPEMGTTGYCWFDRAEVKPFVETIPGATTDVF
QAIASKHRCYIVVGMPEVDPASDLYYNTAVLIGPGGVVGRHRKSHPYIAEPKWAANGDIVHEVFETEIGRISMLVCMDLH
FFETARLEALAGADIICHISNWLQERTPAPYWINRAFENACYVIESNRWGLERTVQFSGGSCLIEPDGTVAASIDTGDGI
AYGTVDLARARRREVLFEPVFKSRRPELYMNMMTNSFTWNPGDYFRLYGYQPIPRGRASRAAVAQFAPTSVVADNLARIA
DLAAEAKATTAPDILVFPELSLTGLEAPHGRAESLSGPTVSAFVRLAMKLGFYLVAGFAEEDGDKVYNSAVLAGPEGLVG
SYRKTHLGIADSWATAGDEWKVYDLAIGRVGLAVGHDALYPEAIRSLALMGCDVVACPSAIAGTFTGSHTGTKIPHNYPI
PKGADPYHWHALRVRGGENNLYFAFANVLDAARGYQGKSAVFGPDSFAFPRQESAILDEDGIAAAAVDTTNLDTPYPTNI
VRRKDLVVMRQPHHYQPLVKWHQ
>Mature_582_residues
GTTMTSTQSYTAATIQFEPTMFEKARNISRLTALCEEAAEAGARLIVTPEMGTTGYCWFDRAEVKPFVETIPGATTDVFQ
AIASKHRCYIVVGMPEVDPASDLYYNTAVLIGPGGVVGRHRKSHPYIAEPKWAANGDIVHEVFETEIGRISMLVCMDLHF
FETARLEALAGADIICHISNWLQERTPAPYWINRAFENACYVIESNRWGLERTVQFSGGSCLIEPDGTVAASIDTGDGIA
YGTVDLARARRREVLFEPVFKSRRPELYMNMMTNSFTWNPGDYFRLYGYQPIPRGRASRAAVAQFAPTSVVADNLARIAD
LAAEAKATTAPDILVFPELSLTGLEAPHGRAESLSGPTVSAFVRLAMKLGFYLVAGFAEEDGDKVYNSAVLAGPEGLVGS
YRKTHLGIADSWATAGDEWKVYDLAIGRVGLAVGHDALYPEAIRSLALMGCDVVACPSAIAGTFTGSHTGTKIPHNYPIP
KGADPYHWHALRVRGGENNLYFAFANVLDAARGYQGKSAVFGPDSFAFPRQESAILDEDGIAAAAVDTTNLDTPYPTNIV
RRKDLVVMRQPHHYQPLVKWHQ

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=283, Percent_Identity=27.5618374558304, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI297632348, Length=292, Percent_Identity=27.7397260273973, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI297632350, Length=292, Percent_Identity=27.7397260273973, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI5031947, Length=292, Percent_Identity=27.7397260273973, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI7706509, Length=246, Percent_Identity=27.6422764227642, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17533173, Length=253, Percent_Identity=29.6442687747036, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17557111, Length=293, Percent_Identity=24.2320819112628, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323383, Length=297, Percent_Identity=24.9158249158249, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21358471, Length=254, Percent_Identity=27.1653543307087, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355835, Length=267, Percent_Identity=27.7153558052434, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: NA

Molecular weight: Translated: 63718; Mature: 63587

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTTMTSTQSYTAATIQFEPTMFEKARNISRLTALCEEAAEAGARLIVTPEMGTTGYCWF
CCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEE
DRAEVKPFVETIPGATTDVFQAIASKHRCYIVVGMPEVDPASDLYYNTAVLIGPGGVVGR
CCCCCCHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEEECCCCCCCC
HRKSHPYIAEPKWAANGDIVHEVFETEIGRISMLVCMDLHFFETARLEALAGADIICHIS
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEHH
NWLQERTPAPYWINRAFENACYVIESNRWGLERTVQFSGGSCLIEPDGTVAASIDTGDGI
HHHHHCCCCCHHHHHHHCCEEEEEECCCCCCEEEEEECCCEEEECCCCCEEEEEECCCCE
AYGTVDLARARRREVLFEPVFKSRRPELYMNMMTNSFTWNPGDYFRLYGYQPIPRGRASR
EECHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCEECCCCCEEEEECCCCCCCCCCCH
AAVAQFAPTSVVADNLARIADLAAEAKATTAPDILVFPELSLTGLEAPHGRAESLSGPTV
HHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEECCCCCCCCCCCCCCCHH
SAFVRLAMKLGFYLVAGFAEEDGDKVYNSAVLAGPEGLVGSYRKTHLGIADSWATAGDEW
HHHHHHHHHHHHHEEEECCCCCCCHHHCCEEEECCCCHHCCCHHHCCCCCCCCCCCCCCE
KVYDLAIGRVGLAVGHDALYPEAIRSLALMGCDVVACPSAIAGTFTGSHTGTKIPHNYPI
EEEEEEECCEEEEECCCCCCHHHHHHHHHHCCCCEECCCHHHCCCCCCCCCCCCCCCCCC
PKGADPYHWHALRVRGGENNLYFAFANVLDAARGYQGKSAVFGPDSFAFPRQESAILDED
CCCCCCCEEEEEEEECCCCCEEEEHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCC
GIAAAAVDTTNLDTPYPTNIVRRKDLVVMRQPHHYQPLVKWHQ
CCEEEEEECCCCCCCCCCCHHCCCCEEEEECCCCCCCHHCCCC
>Mature Secondary Structure 
GTTMTSTQSYTAATIQFEPTMFEKARNISRLTALCEEAAEAGARLIVTPEMGTTGYCWF
CCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEE
DRAEVKPFVETIPGATTDVFQAIASKHRCYIVVGMPEVDPASDLYYNTAVLIGPGGVVGR
CCCCCCHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEEECCCCCCCC
HRKSHPYIAEPKWAANGDIVHEVFETEIGRISMLVCMDLHFFETARLEALAGADIICHIS
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEHH
NWLQERTPAPYWINRAFENACYVIESNRWGLERTVQFSGGSCLIEPDGTVAASIDTGDGI
HHHHHCCCCCHHHHHHHCCEEEEEECCCCCCEEEEEECCCEEEECCCCCEEEEEECCCCE
AYGTVDLARARRREVLFEPVFKSRRPELYMNMMTNSFTWNPGDYFRLYGYQPIPRGRASR
EECHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCEECCCCCEEEEECCCCCCCCCCCH
AAVAQFAPTSVVADNLARIADLAAEAKATTAPDILVFPELSLTGLEAPHGRAESLSGPTV
HHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEECCCCCCCCCCCCCCCHH
SAFVRLAMKLGFYLVAGFAEEDGDKVYNSAVLAGPEGLVGSYRKTHLGIADSWATAGDEW
HHHHHHHHHHHHHEEEECCCCCCCHHHCCEEEECCCCHHCCCHHHCCCCCCCCCCCCCCE
KVYDLAIGRVGLAVGHDALYPEAIRSLALMGCDVVACPSAIAGTFTGSHTGTKIPHNYPI
EEEEEEECCEEEEECCCCCCHHHHHHHHHHCCCCEECCCHHHCCCCCCCCCCCCCCCCCC
PKGADPYHWHALRVRGGENNLYFAFANVLDAARGYQGKSAVFGPDSFAFPRQESAILDED
CCCCCCCEEEEEEEECCCCCEEEEHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCC
GIAAAAVDTTNLDTPYPTNIVRRKDLVVMRQPHHYQPLVKWHQ
CCEEEEEECCCCCCCCCCCHHCCCCEEEEECCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7565609 [H]