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The map label for this gene is hyuB [H]

Identifier: 116249078

GI number: 116249078

Start: 446335

End: 448011

Strand: Direct

Name: hyuB [H]

Synonym: pRL120412

Alternate gene names: 116249078

Gene position: 446335-448011 (Clockwise)

Preceding gene: 116249077

Following gene: 116249079

Centisome position: 51.3

GC content: 61.0

Gene sequence:

>1677_bases
ATGTCGAAACCCGCCACCGATTTCAACGACCCGATCAACCTTCAGGTCATGTGGAACCGCCTGATCTTCATCGCAGACCA
GGCTGACAACGTCCTCGGCAAGACGGCATTCTCGCCGATCGTTCGCGAGAACCACGATTACGTCACCGTCCTGCTGGACA
GCAAGGGACGGGCGCTGGCGCAATGCACCTGGTCGATCCCGGTCTTCATCACCTCGCTTCCGGCCGCGGCCCAGAATTAT
TTCCTGCCGAAGTTCCCGGCAGACAAGCTCGAAGAGGGTGACGTGCTGGCGACCAACGATCCGGAGATCGGTACCGGCCA
TCTTCCCGATGTCACGATGATCACGCCGATCTTCAAGAACGGCAAGGTCGTCGCCTATGCCGGCTCCATCGCCCATCTTC
CCGATATCGGCGGCGCCCCTCTGCATTCCGAAGCGAGCGATATCTACGAAGAAGGCATCCGCTTCCCGATTATCAAGCTG
CTGAAGGCCGGCGTTCCGAACCAGGACGTCTTCGACATCATCGAAGCGTCGGTCCGCCTTCCGACCGAAGTGCGCGGCGA
TCTCGAGTCGATGATCGCTGCCAACAACGTCATGGGCCGCGAACTCGTCAAATTCCTGGATGAGTACGGTCTCGACGACG
TCGAAGGCCTGGCAACCGCCATCCATTCCCGCTCCGAAGCGCAGACCCGCAAGGCGATCCGGGAATGGCCGAACGGCAGC
TACGCCGCCGAGGTTCTGCTCGACGGTTATGATGTCGACGTCACGCTGAAGGCTTCAGTCATTGTCAACGATGACTCGAT
CCATGTCGATTACACCGGCACCTCCGACCAGGTTTTGCACTCGATCAACTGCCGGACGAACTATCGCTACGCCCATTCGG
TCTATGCTCTGAAATGCCTGCTCGACCCCGAGACTCCGAACAACGAGGGCTGCATCGTTCCGATCACCGACGAGGCGCCG
CTCGGCTGCATCCTCAATCCGCAGCACTGGACGGCGGGCAACTCGCGGAACCTGATCGGGCACGTTATTCCCTCGCTGAT
CTTCAAGGCGCTCGAAGGCGTGGTGCCCGAGAAGGTTATGGGCGACAGCGGCGGCGCGCCGATCTGGGCTGCCAATTGCG
TTGGTCAGCGCAATGACGGCACGCAATATGGCTCCGTCCAGAATTTCCATGGCGGGCAGGGCGCGCGCGCGGAGCTCGAC
GGTCTGGATACGCTGAGTTTCCCCTCGAACTGCAAGGTAACGGCCATCGAGATGTTCGAGGTTGCCGTTCCCGTGCTGAC
GGAGCGGAAGGAACTGATCGCGGACTCCGGCGGTGCCGGCAAGCATCGCGGCGGTCTCGGCCAGCGTGTCGTGCTGCGGA
ACCTCGGCAAGAACCCGATGAACATCTACCTCGCCTCCGAGCGTGTTCGCCATCCCTGCTTCGGTGTCGTGGAAGGCCAG
AGCGGATCGGCTGGAAAGGTCATGAAGGATGGCAAGCCTCAGTTCCCGAAGGGCAAGGTGGTTCTGAAGACAGGCCAGCG
CCTCGAGGTCGAGACGCCAGGCGGCGGCGGTTGGGGAGCTGCGAGTAATCGTTCGTCTGCACTCATCGAGCAGGATCTTT
CCGAAAACCTGATTACGGCGAAGGCTGCGAAGGAAATTTACGGCTATTCTGGCCCGCTCGCTGCTGCGGCCGAATAG

Upstream 100 bases:

>100_bases
TCGAGCAGCGTGAATCGACTGCGGTCGTCGGGCCAAGCGGCGCCTTCCATGTGGATGCCCATGGCAACCTCGTGATCAAC
ATTCGCTAAGGGGTACTCAG

Downstream 100 bases:

>100_bases
GGGAAAACCATGGGACTTCTCGAAGGAAAAATCGCTCTCGTGACGGGAGCCGGCTCCGGCATCGGTCGCGAAACCGCCCT
GCTGCTGGCAAAGGACGGCG

Product: putative hydantoin utilization protein b

Products: NA

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MSKPATDFNDPINLQVMWNRLIFIADQADNVLGKTAFSPIVRENHDYVTVLLDSKGRALAQCTWSIPVFITSLPAAAQNY
FLPKFPADKLEEGDVLATNDPEIGTGHLPDVTMITPIFKNGKVVAYAGSIAHLPDIGGAPLHSEASDIYEEGIRFPIIKL
LKAGVPNQDVFDIIEASVRLPTEVRGDLESMIAANNVMGRELVKFLDEYGLDDVEGLATAIHSRSEAQTRKAIREWPNGS
YAAEVLLDGYDVDVTLKASVIVNDDSIHVDYTGTSDQVLHSINCRTNYRYAHSVYALKCLLDPETPNNEGCIVPITDEAP
LGCILNPQHWTAGNSRNLIGHVIPSLIFKALEGVVPEKVMGDSGGAPIWAANCVGQRNDGTQYGSVQNFHGGQGARAELD
GLDTLSFPSNCKVTAIEMFEVAVPVLTERKELIADSGGAGKHRGGLGQRVVLRNLGKNPMNIYLASERVRHPCFGVVEGQ
SGSAGKVMKDGKPQFPKGKVVLKTGQRLEVETPGGGGWGAASNRSSALIEQDLSENLITAKAAKEIYGYSGPLAAAAE

Sequences:

>Translated_558_residues
MSKPATDFNDPINLQVMWNRLIFIADQADNVLGKTAFSPIVRENHDYVTVLLDSKGRALAQCTWSIPVFITSLPAAAQNY
FLPKFPADKLEEGDVLATNDPEIGTGHLPDVTMITPIFKNGKVVAYAGSIAHLPDIGGAPLHSEASDIYEEGIRFPIIKL
LKAGVPNQDVFDIIEASVRLPTEVRGDLESMIAANNVMGRELVKFLDEYGLDDVEGLATAIHSRSEAQTRKAIREWPNGS
YAAEVLLDGYDVDVTLKASVIVNDDSIHVDYTGTSDQVLHSINCRTNYRYAHSVYALKCLLDPETPNNEGCIVPITDEAP
LGCILNPQHWTAGNSRNLIGHVIPSLIFKALEGVVPEKVMGDSGGAPIWAANCVGQRNDGTQYGSVQNFHGGQGARAELD
GLDTLSFPSNCKVTAIEMFEVAVPVLTERKELIADSGGAGKHRGGLGQRVVLRNLGKNPMNIYLASERVRHPCFGVVEGQ
SGSAGKVMKDGKPQFPKGKVVLKTGQRLEVETPGGGGWGAASNRSSALIEQDLSENLITAKAAKEIYGYSGPLAAAAE
>Mature_557_residues
SKPATDFNDPINLQVMWNRLIFIADQADNVLGKTAFSPIVRENHDYVTVLLDSKGRALAQCTWSIPVFITSLPAAAQNYF
LPKFPADKLEEGDVLATNDPEIGTGHLPDVTMITPIFKNGKVVAYAGSIAHLPDIGGAPLHSEASDIYEEGIRFPIIKLL
KAGVPNQDVFDIIEASVRLPTEVRGDLESMIAANNVMGRELVKFLDEYGLDDVEGLATAIHSRSEAQTRKAIREWPNGSY
AAEVLLDGYDVDVTLKASVIVNDDSIHVDYTGTSDQVLHSINCRTNYRYAHSVYALKCLLDPETPNNEGCIVPITDEAPL
GCILNPQHWTAGNSRNLIGHVIPSLIFKALEGVVPEKVMGDSGGAPIWAANCVGQRNDGTQYGSVQNFHGGQGARAELDG
LDTLSFPSNCKVTAIEMFEVAVPVLTERKELIADSGGAGKHRGGLGQRVVLRNLGKNPMNIYLASERVRHPCFGVVEGQS
GSAGKVMKDGKPQFPKGKVVLKTGQRLEVETPGGGGWGAASNRSSALIEQDLSENLITAKAAKEIYGYSGPLAAAAE

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=542, Percent_Identity=28.2287822878229, Blast_Score=152, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI133901900, Length=559, Percent_Identity=28.0858676207513, Blast_Score=168, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI133901902, Length=518, Percent_Identity=27.4131274131274, Blast_Score=142, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6322634, Length=560, Percent_Identity=29.1071428571429, Blast_Score=167, Evalue=5e-42,
Organism=Drosophila melanogaster, GI45550492, Length=572, Percent_Identity=26.5734265734266, Blast_Score=160, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: NA

Molecular weight: Translated: 59995; Mature: 59863

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPATDFNDPINLQVMWNRLIFIADQADNVLGKTAFSPIVRENHDYVTVLLDSKGRALA
CCCCCCCCCCCEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEE
QCTWSIPVFITSLPAAAQNYFLPKFPADKLEEGDVLATNDPEIGTGHLPDVTMITPIFKN
EEEEECEEEEECCCHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEEEEECC
GKVVAYAGSIAHLPDIGGAPLHSEASDIYEEGIRFPIIKLLKAGVPNQDVFDIIEASVRL
CEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHCC
PTEVRGDLESMIAANNVMGRELVKFLDEYGLDDVEGLATAIHSRSEAQTRKAIREWPNGS
CHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCC
YAAEVLLDGYDVDVTLKASVIVNDDSIHVDYTGTSDQVLHSINCRTNYRYAHSVYALKCL
EEEEEEECCCCEEEEEEEEEEECCCEEEEEECCCHHHHHEEECCCCCCEEHHEEEEEEEE
LDPETPNNEGCIVPITDEAPLGCILNPQHWTAGNSRNLIGHVIPSLIFKALEGVVPEKVM
ECCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHC
GDSGGAPIWAANCVGQRNDGTQYGSVQNFHGGQGARAELDGLDTLSFPSNCKVTAIEMFE
CCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEHHH
VAVPVLTERKELIADSGGAGKHRGGLGQRVVLRNLGKNPMNIYLASERVRHPCFGVVEGQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCEEEEECC
SGSAGKVMKDGKPQFPKGKVVLKTGQRLEVETPGGGGWGAASNRSSALIEQDLSENLITA
CCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
KAAKEIYGYSGPLAAAAE
HHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SKPATDFNDPINLQVMWNRLIFIADQADNVLGKTAFSPIVRENHDYVTVLLDSKGRALA
CCCCCCCCCCEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEE
QCTWSIPVFITSLPAAAQNYFLPKFPADKLEEGDVLATNDPEIGTGHLPDVTMITPIFKN
EEEEECEEEEECCCHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEEEEECC
GKVVAYAGSIAHLPDIGGAPLHSEASDIYEEGIRFPIIKLLKAGVPNQDVFDIIEASVRL
CEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHCC
PTEVRGDLESMIAANNVMGRELVKFLDEYGLDDVEGLATAIHSRSEAQTRKAIREWPNGS
CHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCC
YAAEVLLDGYDVDVTLKASVIVNDDSIHVDYTGTSDQVLHSINCRTNYRYAHSVYALKCL
EEEEEEECCCCEEEEEEEEEEECCCEEEEEECCCHHHHHEEECCCCCCEEHHEEEEEEEE
LDPETPNNEGCIVPITDEAPLGCILNPQHWTAGNSRNLIGHVIPSLIFKALEGVVPEKVM
ECCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHC
GDSGGAPIWAANCVGQRNDGTQYGSVQNFHGGQGARAELDGLDTLSFPSNCKVTAIEMFE
CCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEHHH
VAVPVLTERKELIADSGGAGKHRGGLGQRVVLRNLGKNPMNIYLASERVRHPCFGVVEGQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCEEEEECC
SGSAGKVMKDGKPQFPKGKVVLKTGQRLEVETPGGGGWGAASNRSSALIEQDLSENLITA
CCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
KAAKEIYGYSGPLAAAAE
HHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]