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The map label for this gene is hyuA [H]

Identifier: 116249077

GI number: 116249077

Start: 444260

End: 446323

Strand: Direct

Name: hyuA [H]

Synonym: pRL120411

Alternate gene names: 116249077

Gene position: 444260-446323 (Clockwise)

Preceding gene: 116249076

Following gene: 116249078

Centisome position: 51.06

GC content: 63.32

Gene sequence:

>2064_bases
ATGGCAAAACTGGCTTTCGACACGGGCGGTACCTTTACCGACTTCGCGCTCCTGGACGACAAGGGCGACCTTCACCTTCA
CAAGGTCCTGAGCACGCCAAAGAATCCTGCAGAAGCAGTCGTGCAGGGTGTGTCCGAATTGCTGGAAAAATTCGCGGGCA
CCATCGCCATCGAGAACCTGCAAGTGCTGGGCGCCACCACCGTCGTCACCAACGCGGTTCTCGAGCGCAAGGGCGTCGAG
ACGGGGTTCGTCACCACCGACGGTTTTCAGGACATGCTGCGTATCCGCAACGAAGGGCGCTACGACCTCTACGACCTGAA
TATCAAATATCCCGACCCGCTGGTCACCCGCGCAAACAGCTTCGGCGCCGAAGAGCGTATCGCGGCTGACGGTTCTGTCA
TGACCGAGCTCAAGGAAGAGACGGTGCGAGAAATCGCCGGCCGCCTGAAGGAGAAGGGCATCCGCTCCGTCGCCGTCTGC
CTTCTCCATGCCTACAAGTATCCCCAGCACGAGCAGCGCGTCGCAGCGCTGCTGCGCGAAGAACACCCTGACATCTTCGT
CTCGCTTTCTTCCGAAGTCTGCCCTGAGATGCGCGAGTTCGATCGCGCGTCGACGACTGTTGTCAATGCCTATACCAGGC
CGCAGATGGCTGGGCACGTCGCTCATCTCCAGCGTGAGTTCGCTGCGAAGGGCATCGATCGGCAGGTCCTCTGGATGACG
TCATCGGGCGGGCTGGTGCCGAGCCGGCGTGCCGCAGAACTGCCGGTACGCCTGATCGAGTCAGGCCCTGCGGCCGGCGC
CGTGGCCGCTGCCGAGTTCGGCCGCACGGCAGGTGAGGCGAGTGTCCTGTCCTTCGACATGGGCGGCACGACCGCGAAGC
TCTGCCTGATCCCCGACGGTGAGCCGAATGTCGGCACCGACCTCGAAGTCGCCCATTATCAGCGCTTCCGCAAAGGATCC
GGTTTCCCGCTGAAGATCCAGTCGATCCAGATGATCGAGATCGGCGCGGGCGGCGGTTCGATTGCGGCCAAAAACCCTCT
CGGTCTGCTTGACGTCGGCCCGCACTCTGCAGGCGCCATGCCCGGGCCGGCCGCCTACCAGCGCGGCGGCACCCAGCCGA
CCGTCACGGATGCCGACATCCTGCTCGGTTACATGGGCACCGAATCCTTCGTCGGCGGCTCGTTCAAAGTTTCCAAGGAA
GCCGCCCACGAGGCGATGGCCAAACTTGCCGCATCGCTCGAGGTCTCGGTCGAACGCTGCGCATGGGGCATCCATGACCT
CGTCAACGAATCCATGAGCAAGGCGGCCGCCATGCATGCGACCGATCTCGGCGTCGATCCCCGTTCGCTGCCCATGGTCG
CGTTCGGCGGCGCCGGTCCGGTCCACGCCTATGGCATCGCCCGCAAGCTCGGCATTTCCCGCATCATCTGCCCGACCGGT
GCAGGCGTGAGCTCGGCGATCGGCCTGTTGATCGCTCCCGTGGCGGTCGATCTGTCCGCAAGCCATCCGATGGCACTCGA
CGCTTGGAATATGGACGCCATGAACCGTCTGCTGGACGAACTCTCGGCCCAAGGTGCCGAAGTCGTGTCGGCGGCGGGCG
TGCCGAAGGATACGATCAGCAACCGCTACACCGTCGACATGCGCCATGTCGGCCAGGGCCACGAGATCACCGTGGTTCTG
CCTGACCGGAACCTGCCGAAGGAGGAATTCCTCAGTGAGCTCCGTGGCAATTTCTTCAAGCTGTACCATGAACTGTTTGG
CCGTACCGTTGCCGCGCCGCTGGAGGTCATCACCTGGCGCCTGCGCGCCGGCGGCAAAAAAGATCAGGTCACGCGCCCGC
ACGAGACCGTCGTTGCCGACGCGAAGAAGGGCAGCCGCAAGGTCTACTTCCAGGAGATCGGCGGCTTTACCGAGACTGCG
GTCTACGACCATTACAAGCTTCCCGTCGGCGAAGAGGTCAGGGGTCCGGCAATCGTCGAGCAGCGTGAATCGACTGCGGT
CGTCGGGCCAAGCGGCGCCTTCCATGTGGATGCCCATGGCAACCTCGTGATCAACATTCGCTAA

Upstream 100 bases:

>100_bases
ACGCGCCGGGCATTCCCCTAACCTTCAAGCAGACACCGGGTCCGGGCATCGGATCCGCATAGCGGCGCCTGCCGCGCCGA
ACAGTCGCTTCGAGGACAAC

Downstream 100 bases:

>100_bases
GGGGTACTCAGATGTCGAAACCCGCCACCGATTTCAACGACCCGATCAACCTTCAGGTCATGTGGAACCGCCTGATCTTC
ATCGCAGACCAGGCTGACAA

Product: putative hydantoin utilization protein a

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 687; Mature: 686

Protein sequence:

>687_residues
MAKLAFDTGGTFTDFALLDDKGDLHLHKVLSTPKNPAEAVVQGVSELLEKFAGTIAIENLQVLGATTVVTNAVLERKGVE
TGFVTTDGFQDMLRIRNEGRYDLYDLNIKYPDPLVTRANSFGAEERIAADGSVMTELKEETVREIAGRLKEKGIRSVAVC
LLHAYKYPQHEQRVAALLREEHPDIFVSLSSEVCPEMREFDRASTTVVNAYTRPQMAGHVAHLQREFAAKGIDRQVLWMT
SSGGLVPSRRAAELPVRLIESGPAAGAVAAAEFGRTAGEASVLSFDMGGTTAKLCLIPDGEPNVGTDLEVAHYQRFRKGS
GFPLKIQSIQMIEIGAGGGSIAAKNPLGLLDVGPHSAGAMPGPAAYQRGGTQPTVTDADILLGYMGTESFVGGSFKVSKE
AAHEAMAKLAASLEVSVERCAWGIHDLVNESMSKAAAMHATDLGVDPRSLPMVAFGGAGPVHAYGIARKLGISRIICPTG
AGVSSAIGLLIAPVAVDLSASHPMALDAWNMDAMNRLLDELSAQGAEVVSAAGVPKDTISNRYTVDMRHVGQGHEITVVL
PDRNLPKEEFLSELRGNFFKLYHELFGRTVAAPLEVITWRLRAGGKKDQVTRPHETVVADAKKGSRKVYFQEIGGFTETA
VYDHYKLPVGEEVRGPAIVEQRESTAVVGPSGAFHVDAHGNLVINIR

Sequences:

>Translated_687_residues
MAKLAFDTGGTFTDFALLDDKGDLHLHKVLSTPKNPAEAVVQGVSELLEKFAGTIAIENLQVLGATTVVTNAVLERKGVE
TGFVTTDGFQDMLRIRNEGRYDLYDLNIKYPDPLVTRANSFGAEERIAADGSVMTELKEETVREIAGRLKEKGIRSVAVC
LLHAYKYPQHEQRVAALLREEHPDIFVSLSSEVCPEMREFDRASTTVVNAYTRPQMAGHVAHLQREFAAKGIDRQVLWMT
SSGGLVPSRRAAELPVRLIESGPAAGAVAAAEFGRTAGEASVLSFDMGGTTAKLCLIPDGEPNVGTDLEVAHYQRFRKGS
GFPLKIQSIQMIEIGAGGGSIAAKNPLGLLDVGPHSAGAMPGPAAYQRGGTQPTVTDADILLGYMGTESFVGGSFKVSKE
AAHEAMAKLAASLEVSVERCAWGIHDLVNESMSKAAAMHATDLGVDPRSLPMVAFGGAGPVHAYGIARKLGISRIICPTG
AGVSSAIGLLIAPVAVDLSASHPMALDAWNMDAMNRLLDELSAQGAEVVSAAGVPKDTISNRYTVDMRHVGQGHEITVVL
PDRNLPKEEFLSELRGNFFKLYHELFGRTVAAPLEVITWRLRAGGKKDQVTRPHETVVADAKKGSRKVYFQEIGGFTETA
VYDHYKLPVGEEVRGPAIVEQRESTAVVGPSGAFHVDAHGNLVINIR
>Mature_686_residues
AKLAFDTGGTFTDFALLDDKGDLHLHKVLSTPKNPAEAVVQGVSELLEKFAGTIAIENLQVLGATTVVTNAVLERKGVET
GFVTTDGFQDMLRIRNEGRYDLYDLNIKYPDPLVTRANSFGAEERIAADGSVMTELKEETVREIAGRLKEKGIRSVAVCL
LHAYKYPQHEQRVAALLREEHPDIFVSLSSEVCPEMREFDRASTTVVNAYTRPQMAGHVAHLQREFAAKGIDRQVLWMTS
SGGLVPSRRAAELPVRLIESGPAAGAVAAAEFGRTAGEASVLSFDMGGTTAKLCLIPDGEPNVGTDLEVAHYQRFRKGSG
FPLKIQSIQMIEIGAGGGSIAAKNPLGLLDVGPHSAGAMPGPAAYQRGGTQPTVTDADILLGYMGTESFVGGSFKVSKEA
AHEAMAKLAASLEVSVERCAWGIHDLVNESMSKAAAMHATDLGVDPRSLPMVAFGGAGPVHAYGIARKLGISRIICPTGA
GVSSAIGLLIAPVAVDLSASHPMALDAWNMDAMNRLLDELSAQGAEVVSAAGVPKDTISNRYTVDMRHVGQGHEITVVLP
DRNLPKEEFLSELRGNFFKLYHELFGRTVAAPLEVITWRLRAGGKKDQVTRPHETVVADAKKGSRKVYFQEIGGFTETAV
YDHYKLPVGEEVRGPAIVEQRESTAVVGPSGAFHVDAHGNLVINIR

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0145

COG function: function code EQ; N-methylhydantoinase A/acetone carboxylase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=743, Percent_Identity=29.878869448183, Blast_Score=223, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI133901900, Length=723, Percent_Identity=26.9709543568465, Blast_Score=207, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6322634, Length=759, Percent_Identity=27.6679841897233, Blast_Score=197, Evalue=6e-51,
Organism=Drosophila melanogaster, GI45550492, Length=755, Percent_Identity=29.4039735099338, Blast_Score=224, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008040
- InterPro:   IPR002821 [H]

Pfam domain/function: PF05378 Hydant_A_N; PF01968 Hydantoinase_A [H]

EC number: 3.5.2.14

Molecular weight: Translated: 73603; Mature: 73472

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLAFDTGGTFTDFALLDDKGDLHLHKVLSTPKNPAEAVVQGVSELLEKFAGTIAIENL
CCEEEECCCCCEEEEEEECCCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCEEEEECE
QVLGATTVVTNAVLERKGVETGFVTTDGFQDMLRIRNEGRYDLYDLNIKYPDPLVTRANS
EEECHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHCCCCEEEEEEEEECCCHHHHCCCC
FGAEERIAADGSVMTELKEETVREIAGRLKEKGIRSVAVCLLHAYKYPQHEQRVAALLRE
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EHPDIFVSLSSEVCPEMREFDRASTTVVNAYTRPQMAGHVAHLQREFAAKGIDRQVLWMT
CCCCEEEEECHHHCHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
SSGGLVPSRRAAELPVRLIESGPAAGAVAAAEFGRTAGEASVLSFDMGGTTAKLCLIPDG
CCCCCCCCCCHHHCCHHHCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCEEEEEEECCC
EPNVGTDLEVAHYQRFRKGSGFPLKIQSIQMIEIGAGGGSIAAKNPLGLLDVGPHSAGAM
CCCCCCCCCHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCEEECCCCCCCCC
PGPAAYQRGGTQPTVTDADILLGYMGTESFVGGSFKVSKEAAHEAMAKLAASLEVSVERC
CCCHHHHCCCCCCCCCCHHHHEEECCCHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHH
AWGIHDLVNESMSKAAAMHATDLGVDPRSLPMVAFGGAGPVHAYGIARKLGISRIICPTG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCEEECCCC
AGVSSAIGLLIAPVAVDLSASHPMALDAWNMDAMNRLLDELSAQGAEVVSAAGVPKDTIS
CCHHHHHHHHHHHHEEECCCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHCCCCHHHHC
NRYTVDMRHVGQGHEITVVLPDRNLPKEEFLSELRGNFFKLYHELFGRTVAAPLEVITWR
CCEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCHHHHHEEH
LRAGGKKDQVTRPHETVVADAKKGSRKVYFQEIGGFTETAVYDHYKLPVGEEVRGPAIVE
HHCCCCCCCCCCCHHHHEECCCCCCCEEEHHHHCCCHHHHEECCEECCCCCCCCCCHHHC
QRESTAVVGPSGAFHVDAHGNLVINIR
CCCCCEEECCCCCEEEECCCCEEEEEC
>Mature Secondary Structure 
AKLAFDTGGTFTDFALLDDKGDLHLHKVLSTPKNPAEAVVQGVSELLEKFAGTIAIENL
CEEEECCCCCEEEEEEECCCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCEEEEECE
QVLGATTVVTNAVLERKGVETGFVTTDGFQDMLRIRNEGRYDLYDLNIKYPDPLVTRANS
EEECHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHCCCCEEEEEEEEECCCHHHHCCCC
FGAEERIAADGSVMTELKEETVREIAGRLKEKGIRSVAVCLLHAYKYPQHEQRVAALLRE
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EHPDIFVSLSSEVCPEMREFDRASTTVVNAYTRPQMAGHVAHLQREFAAKGIDRQVLWMT
CCCCEEEEECHHHCHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
SSGGLVPSRRAAELPVRLIESGPAAGAVAAAEFGRTAGEASVLSFDMGGTTAKLCLIPDG
CCCCCCCCCCHHHCCHHHCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCEEEEEEECCC
EPNVGTDLEVAHYQRFRKGSGFPLKIQSIQMIEIGAGGGSIAAKNPLGLLDVGPHSAGAM
CCCCCCCCCHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCEEECCCCCCCCC
PGPAAYQRGGTQPTVTDADILLGYMGTESFVGGSFKVSKEAAHEAMAKLAASLEVSVERC
CCCHHHHCCCCCCCCCCHHHHEEECCCHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHH
AWGIHDLVNESMSKAAAMHATDLGVDPRSLPMVAFGGAGPVHAYGIARKLGISRIICPTG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCEEECCCC
AGVSSAIGLLIAPVAVDLSASHPMALDAWNMDAMNRLLDELSAQGAEVVSAAGVPKDTIS
CCHHHHHHHHHHHHEEECCCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHCCCCHHHHC
NRYTVDMRHVGQGHEITVVLPDRNLPKEEFLSELRGNFFKLYHELFGRTVAAPLEVITWR
CCEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCHHHHHEEH
LRAGGKKDQVTRPHETVVADAKKGSRKVYFQEIGGFTETAVYDHYKLPVGEEVRGPAIVE
HHCCCCCCCCCCCHHHHEECCCCCCCEEEHHHHCCCHHHHEECCEECCCCCCCCCCHHHC
QRESTAVVGPSGAFHVDAHGNLVINIR
CCCCCEEECCCCCEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]