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The map label for this gene is ycdT [H]

Identifier: 116248992

GI number: 116248992

Start: 346868

End: 348154

Strand: Direct

Name: ycdT [H]

Synonym: pRL120324

Alternate gene names: 116248992

Gene position: 346868-348154 (Clockwise)

Preceding gene: 116248989

Following gene: 116248993

Centisome position: 39.87

GC content: 57.96

Gene sequence:

>1287_bases
ATGGATACCGAGCGAACCACAGCTCCTATCAAACCGGTAAGGGCGTCTTCGCGGAAACCTCTTCAGAGGTTGAGCCCGGC
CATTGTCGAGCAGGTCGAGCGCCTGCTCGGTGGACGCACGCGTGATATCCGCTTCAATGGTGAACTGGCCCTGCTTTTTC
GCGAACGCTCCTGGCCGCGGACTGCCAAGATCATCCGCGCGTGGATGGTTTGGGTAATCGTGCTCGACGTTCTGACGTTG
GGCCTCAACGCGGTCTTGCTTCCGACCGAAACTGTGATGTCGATGCTTTGGCCAGCGTCTATCCTTCCCCCCGCAGCTCT
TGTCGCATCGATAGCCTTCCTGCGGCCGCATGCCTTGTGGGTGCAGGGAGTTTTCATCCTCTCGGCTGTTTTTCTCATCC
TCCTGTCGGTCGCCTTGGTGGGCGTTAACACCGGTGGCGAGTTTTACGAGCGGCATTTGAACATCATGCTTTTTGTGGCC
GTCACCGCCATTATCATATTTCCCGTTCCACTCGGTTGGGCGATGGCGATCGGTGCTACTGCCCTCGGTATCTACCTTGT
GTTTCAGCTGCGCAATCCAGACATTAAAGCGGGGAGTGCTCTGGCCGGTACGCTGTTTTTCGCAAGCGGTGTGGCTGCAA
CCGTCGTTGCCCGACGCACCGCAACCATCTTCGCTCAGAAGACTTTCTTGCTCGAGTTACGAGATCGAAGTCGCCTCGCG
GAGCTTACCGACGCCAACTCGCAGCTTGAATTGCTGGCGCGGACCGATCCGCTGACGGGTGTCGCCAACCGGCGCTCGAT
GATGGAAACGCTCCATTATTTCTGGAGCGAGGATCTTAAACGAACGAGCGGCGCGGCGATGCTGATGTGCGATATCGACG
ACTTCAAGCATCTCAACGATAATCTCGGACATGCCGAAGGCGACCGCTGCCTGGTGAAAGTTGCCGGCATCATTCAGAGC
AGCATGAGAGACGAGCGGGACCAGGTCGCCCGGTACGGAGGCGAAGAATTTCTCGTCTTTCTCCCAGGCTCTGATGAACA
AGAGGCCGGGGTGATCGCTGAGAGAATTCGCAGCCGAGTAGAAGCTGCGTCTCTTCCAAATCCTACCTCCCGCGTCGGCC
CCTGGGTCACTGTCAGCATCGGAGTAGCGATATTGATGCAAAACAGCGGACTCGTGTCAGCAGAGCACATGCAGCGACAA
GCGGATGCGGCGCTCTACCTTGCCAAAAAGACCGGCCGCAACTGCGTGGTGGTCCATGCTTCAGAGGTCGGTCAAGCCTC
CGGCTGA

Upstream 100 bases:

>100_bases
TCGTGAAGTGGTCCAAAATCATTAGGGCAAGCATATCGTCTTTTGCGGCGCAATTTGCGGCTCGTCTTGTCTCCGGTCGT
CTGCTCCCTAGATCCGACAC

Downstream 100 bases:

>100_bases
GGTCATTCCAGAAGAACTGTGCTGTTTGCCTGTTGTCGTTCTGGCGCCAAGAACGACCGCTTCAAACGGCTGCGAGCGGA
GGGGCCAGCCATTCCAAGCC

Product: GGDEF domain-containing regulatory protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MDTERTTAPIKPVRASSRKPLQRLSPAIVEQVERLLGGRTRDIRFNGELALLFRERSWPRTAKIIRAWMVWVIVLDVLTL
GLNAVLLPTETVMSMLWPASILPPAALVASIAFLRPHALWVQGVFILSAVFLILLSVALVGVNTGGEFYERHLNIMLFVA
VTAIIIFPVPLGWAMAIGATALGIYLVFQLRNPDIKAGSALAGTLFFASGVAATVVARRTATIFAQKTFLLELRDRSRLA
ELTDANSQLELLARTDPLTGVANRRSMMETLHYFWSEDLKRTSGAAMLMCDIDDFKHLNDNLGHAEGDRCLVKVAGIIQS
SMRDERDQVARYGGEEFLVFLPGSDEQEAGVIAERIRSRVEAASLPNPTSRVGPWVTVSIGVAILMQNSGLVSAEHMQRQ
ADAALYLAKKTGRNCVVVHASEVGQASG

Sequences:

>Translated_428_residues
MDTERTTAPIKPVRASSRKPLQRLSPAIVEQVERLLGGRTRDIRFNGELALLFRERSWPRTAKIIRAWMVWVIVLDVLTL
GLNAVLLPTETVMSMLWPASILPPAALVASIAFLRPHALWVQGVFILSAVFLILLSVALVGVNTGGEFYERHLNIMLFVA
VTAIIIFPVPLGWAMAIGATALGIYLVFQLRNPDIKAGSALAGTLFFASGVAATVVARRTATIFAQKTFLLELRDRSRLA
ELTDANSQLELLARTDPLTGVANRRSMMETLHYFWSEDLKRTSGAAMLMCDIDDFKHLNDNLGHAEGDRCLVKVAGIIQS
SMRDERDQVARYGGEEFLVFLPGSDEQEAGVIAERIRSRVEAASLPNPTSRVGPWVTVSIGVAILMQNSGLVSAEHMQRQ
ADAALYLAKKTGRNCVVVHASEVGQASG
>Mature_428_residues
MDTERTTAPIKPVRASSRKPLQRLSPAIVEQVERLLGGRTRDIRFNGELALLFRERSWPRTAKIIRAWMVWVIVLDVLTL
GLNAVLLPTETVMSMLWPASILPPAALVASIAFLRPHALWVQGVFILSAVFLILLSVALVGVNTGGEFYERHLNIMLFVA
VTAIIIFPVPLGWAMAIGATALGIYLVFQLRNPDIKAGSALAGTLFFASGVAATVVARRTATIFAQKTFLLELRDRSRLA
ELTDANSQLELLARTDPLTGVANRRSMMETLHYFWSEDLKRTSGAAMLMCDIDDFKHLNDNLGHAEGDRCLVKVAGIIQS
SMRDERDQVARYGGEEFLVFLPGSDEQEAGVIAERIRSRVEAASLPNPTSRVGPWVTVSIGVAILMQNSGLVSAEHMQRQ
ADAALYLAKKTGRNCVVVHASEVGQASG

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=173, Percent_Identity=41.0404624277457, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87081881, Length=195, Percent_Identity=35.3846153846154, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1786584, Length=174, Percent_Identity=32.183908045977, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082007, Length=173, Percent_Identity=36.9942196531792, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI145693134, Length=193, Percent_Identity=35.2331606217617, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787802, Length=183, Percent_Identity=36.6120218579235, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787541, Length=188, Percent_Identity=32.9787234042553, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788085, Length=168, Percent_Identity=32.7380952380952, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788381, Length=181, Percent_Identity=33.7016574585635, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788956, Length=169, Percent_Identity=35.5029585798817, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787056, Length=164, Percent_Identity=31.0975609756098, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87081977, Length=250, Percent_Identity=28.4, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081974, Length=157, Percent_Identity=29.9363057324841, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787816, Length=178, Percent_Identity=29.7752808988764, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 46852; Mature: 46852

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS50887 GGDEF ; PS00284 SERPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTERTTAPIKPVRASSRKPLQRLSPAIVEQVERLLGGRTRDIRFNGELALLFRERSWPR
CCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCH
TAKIIRAWMVWVIVLDVLTLGLNAVLLPTETVMSMLWPASILPPAALVASIAFLRPHALW
HHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHCCHHCCCHHHHHHHHHHHCHHHHH
VQGVFILSAVFLILLSVALVGVNTGGEFYERHLNIMLFVAVTAIIIFPVPLGWAMAIGAT
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
ALGIYLVFQLRNPDIKAGSALAGTLFFASGVAATVVARRTATIFAQKTFLLELRDRSRLA
HHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHH
ELTDANSQLELLARTDPLTGVANRRSMMETLHYFWSEDLKRTSGAAMLMCDIDDFKHLND
HHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHCC
NLGHAEGDRCLVKVAGIIQSSMRDERDQVARYGGEEFLVFLPGSDEQEAGVIAERIRSRV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
EAASLPNPTSRVGPWVTVSIGVAILMQNSGLVSAEHMQRQADAALYLAKKTGRNCVVVHA
HHHCCCCCCHHCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHEEEECCCCCEEEEEEE
SEVGQASG
CCCCCCCC
>Mature Secondary Structure
MDTERTTAPIKPVRASSRKPLQRLSPAIVEQVERLLGGRTRDIRFNGELALLFRERSWPR
CCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCH
TAKIIRAWMVWVIVLDVLTLGLNAVLLPTETVMSMLWPASILPPAALVASIAFLRPHALW
HHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHCCHHCCCHHHHHHHHHHHCHHHHH
VQGVFILSAVFLILLSVALVGVNTGGEFYERHLNIMLFVAVTAIIIFPVPLGWAMAIGAT
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
ALGIYLVFQLRNPDIKAGSALAGTLFFASGVAATVVARRTATIFAQKTFLLELRDRSRLA
HHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHH
ELTDANSQLELLARTDPLTGVANRRSMMETLHYFWSEDLKRTSGAAMLMCDIDDFKHLND
HHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHCC
NLGHAEGDRCLVKVAGIIQSSMRDERDQVARYGGEEFLVFLPGSDEQEAGVIAERIRSRV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
EAASLPNPTSRVGPWVTVSIGVAILMQNSGLVSAEHMQRQADAALYLAKKTGRNCVVVHA
HHHCCCCCCHHCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHEEEECCCCCEEEEEEE
SEVGQASG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]