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The map label for this gene is mepA [H]

Identifier: 116248986

GI number: 116248986

Start: 339572

End: 340993

Strand: Direct

Name: mepA [H]

Synonym: pRL120317

Alternate gene names: 116248986

Gene position: 339572-340993 (Clockwise)

Preceding gene: 116248985

Following gene: 116248987

Centisome position: 39.03

GC content: 62.73

Gene sequence:

>1422_bases
ATGAACGATATGAGCGACACAAGCGGCAGCTTCCGGAAACGGCAGCGCCTCGCACCGGAGGACCTTGCCAGTCCTGAGCT
CCTCAGGCTGCTGCTTCGGCTCGGACTGCCGGCCATGTTCGGCCTGTCGATCAACGCCGCCCATCACACGATCAACATGA
TTTTCGTCGGGATGATCGGTGACGACCAAATCGCGGCGATCATGATCGTCCTGCCGGTCCTCATGCTCGTCGCCGCCTTC
GGCGAAGGGATCGGCGTCGGTGTCGCAACAGAGGTCGGACGCACGCTCGGCGCCGGCAACCGATCCCGGGCCGGCGCCAT
TGCCTCGATCAGCCTTGCTGCCGGCGTCGTCTTCGGCGCGGCAAGCGCCATCGCAATCCTCGCATTTCCGGATTTGCTGC
TGTTTGGCGCCACGCCTGCCCTTCAGCCGCTCGCGCAACATTACCTGATGATCATTGCGGTCTCGGTGCCGCTGACGATG
GCGCAGATCATTCTCGATTTCCTGGCAATAGCAGAGGGCAATGCCCGTTTCAGCATGTGGACGCTGGTCGCCTGCTTTGC
GCTGAACATGATCCTCGACCCGATCATGATCTTCGGCTTCGGCCTCGGCCTGCAGGGCGTTGCAATCGCAACCATCCTGT
CCCAGCTCGTCGCGCTTTGCATCTATGCTGCTTACCACGCCAGGCGACTTGGGACCGTCCGGCTGACCTTTGGCTGGCGG
TGGAGAGATATCGGCTATCTCAGGCCTGTGCTTGCTGTGGGCGCACCGACGACACTGACCAGTCTCGCAACCGCAGGCGC
GATTGCGGCCGTGGTATCGCTCGCCGGCACCTATCACGGAGAAGCCGGCATTGCCGGCGTCGGGATCGCCTTGCGGCTGC
TGGCGGTCGGAACCCTCCCCGTCATCGGCATTTCACTCGGAGCCCAGTCGATCCTGAGCTTTGCCTGGGGTCGAGGCGAT
ACGACCCGGATGCTGTCGGCAGCCCGCATCCTCACAGTGGTCACCAGTGCGGTGAGTGGCGCATACGGGTTGGGGGCGCT
CGTTTTCTCCGAAGAGCTTGCCTCGTTCTTCACCGACGATGCCACGGTGATCGGGATCGCCGGGCAAGCGATCATTGCGA
CCCATCTTCCCCTTTTGCTGTTCGGCCTGCGGCAAACGGTGCTGATCCTCTTCCAGGCCCAGGGCAGACCGAAAGCGGCG
GTTGCCATCGGCCTGGCGCAGAATGGCTATCTTCTTTTTCCGCTGCTTGCTCTTCTGCCGCCGTTCTTCGGTTTTTCAGG
CTTGCTGGCAGCAATGTTCCTGGCCTCTGCCTTGACCGGCCTGCTGTCAGCCGGCTGCCTGGCGAGGACGCTCAGCGCGC
TCCGTCAGCGCGCTGCCGACCATCTGACCTCCATCCGTCCATATCCCAACTTTTGTCCATGA

Upstream 100 bases:

>100_bases
TTGCCAAGGTCAAGCATTTCGATTTTCCGCATAAGCGGGCGCTCCTGGTGATGCTCGGCCGCGAGCGTTTTTTCGGCGAT
CGTCTCTGGGTGCCTGCGTC

Downstream 100 bases:

>100_bases
GAGGATGACCATGAACCAGTCGATCAACCCGCACGACCTTGGCGCCCATGCGGCCGCTGACGATCTCTTTACGCCTGTCG
ATCCGGCGGCATTCAACGCC

Product: putative efflux transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MNDMSDTSGSFRKRQRLAPEDLASPELLRLLLRLGLPAMFGLSINAAHHTINMIFVGMIGDDQIAAIMIVLPVLMLVAAF
GEGIGVGVATEVGRTLGAGNRSRAGAIASISLAAGVVFGAASAIAILAFPDLLLFGATPALQPLAQHYLMIIAVSVPLTM
AQIILDFLAIAEGNARFSMWTLVACFALNMILDPIMIFGFGLGLQGVAIATILSQLVALCIYAAYHARRLGTVRLTFGWR
WRDIGYLRPVLAVGAPTTLTSLATAGAIAAVVSLAGTYHGEAGIAGVGIALRLLAVGTLPVIGISLGAQSILSFAWGRGD
TTRMLSAARILTVVTSAVSGAYGLGALVFSEELASFFTDDATVIGIAGQAIIATHLPLLLFGLRQTVLILFQAQGRPKAA
VAIGLAQNGYLLFPLLALLPPFFGFSGLLAAMFLASALTGLLSAGCLARTLSALRQRAADHLTSIRPYPNFCP

Sequences:

>Translated_473_residues
MNDMSDTSGSFRKRQRLAPEDLASPELLRLLLRLGLPAMFGLSINAAHHTINMIFVGMIGDDQIAAIMIVLPVLMLVAAF
GEGIGVGVATEVGRTLGAGNRSRAGAIASISLAAGVVFGAASAIAILAFPDLLLFGATPALQPLAQHYLMIIAVSVPLTM
AQIILDFLAIAEGNARFSMWTLVACFALNMILDPIMIFGFGLGLQGVAIATILSQLVALCIYAAYHARRLGTVRLTFGWR
WRDIGYLRPVLAVGAPTTLTSLATAGAIAAVVSLAGTYHGEAGIAGVGIALRLLAVGTLPVIGISLGAQSILSFAWGRGD
TTRMLSAARILTVVTSAVSGAYGLGALVFSEELASFFTDDATVIGIAGQAIIATHLPLLLFGLRQTVLILFQAQGRPKAA
VAIGLAQNGYLLFPLLALLPPFFGFSGLLAAMFLASALTGLLSAGCLARTLSALRQRAADHLTSIRPYPNFCP
>Mature_473_residues
MNDMSDTSGSFRKRQRLAPEDLASPELLRLLLRLGLPAMFGLSINAAHHTINMIFVGMIGDDQIAAIMIVLPVLMLVAAF
GEGIGVGVATEVGRTLGAGNRSRAGAIASISLAAGVVFGAASAIAILAFPDLLLFGATPALQPLAQHYLMIIAVSVPLTM
AQIILDFLAIAEGNARFSMWTLVACFALNMILDPIMIFGFGLGLQGVAIATILSQLVALCIYAAYHARRLGTVRLTFGWR
WRDIGYLRPVLAVGAPTTLTSLATAGAIAAVVSLAGTYHGEAGIAGVGIALRLLAVGTLPVIGISLGAQSILSFAWGRGD
TTRMLSAARILTVVTSAVSGAYGLGALVFSEELASFFTDDATVIGIAGQAIIATHLPLLLFGLRQTVLILFQAQGRPKAA
VAIGLAQNGYLLFPLLALLPPFFGFSGLLAAMFLASALTGLLSAGCLARTLSALRQRAADHLTSIRPYPNFCP

Specific function: Multidrug resistance efflux protein [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MepA subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR015522 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49237; Mature: 49237

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDMSDTSGSFRKRQRLAPEDLASPELLRLLLRLGLPAMFGLSINAAHHTINMIFVGMIG
CCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHCCEEEHHHHEEEEEEEEEEC
DDQIAAIMIVLPVLMLVAAFGEGIGVGVATEVGRTLGAGNRSRAGAIASISLAAGVVFGA
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
ASAIAILAFPDLLLFGATPALQPLAQHYLMIIAVSVPLTMAQIILDFLAIAEGNARFSMW
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
TLVACFALNMILDPIMIFGFGLGLQGVAIATILSQLVALCIYAAYHARRLGTVRLTFGWR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECEE
WRDIGYLRPVLAVGAPTTLTSLATAGAIAAVVSLAGTYHGEAGIAGVGIALRLLAVGTLP
ECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
VIGISLGAQSILSFAWGRGDTTRMLSAARILTVVTSAVSGAYGLGALVFSEELASFFTDD
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
ATVIGIAGQAIIATHLPLLLFGLRQTVLILFQAQGRPKAAVAIGLAQNGYLLFPLLALLP
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHH
PFFGFSGLLAAMFLASALTGLLSAGCLARTLSALRQRAADHLTSIRPYPNFCP
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNDMSDTSGSFRKRQRLAPEDLASPELLRLLLRLGLPAMFGLSINAAHHTINMIFVGMIG
CCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHCCEEEHHHHEEEEEEEEEEC
DDQIAAIMIVLPVLMLVAAFGEGIGVGVATEVGRTLGAGNRSRAGAIASISLAAGVVFGA
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
ASAIAILAFPDLLLFGATPALQPLAQHYLMIIAVSVPLTMAQIILDFLAIAEGNARFSMW
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
TLVACFALNMILDPIMIFGFGLGLQGVAIATILSQLVALCIYAAYHARRLGTVRLTFGWR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECEE
WRDIGYLRPVLAVGAPTTLTSLATAGAIAAVVSLAGTYHGEAGIAGVGIALRLLAVGTLP
ECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
VIGISLGAQSILSFAWGRGDTTRMLSAARILTVVTSAVSGAYGLGALVFSEELASFFTDD
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
ATVIGIAGQAIIATHLPLLLFGLRQTVLILFQAQGRPKAAVAIGLAQNGYLLFPLLALLP
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHH
PFFGFSGLLAAMFLASALTGLLSAGCLARTLSALRQRAADHLTSIRPYPNFCP
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA