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The map label for this gene is metC [H]

Identifier: 116248987

GI number: 116248987

Start: 340998

End: 342191

Strand: Direct

Name: metC [H]

Synonym: pRL120318

Alternate gene names: 116248987

Gene position: 340998-342191 (Clockwise)

Preceding gene: 116248986

Following gene: 116248989

Centisome position: 39.19

GC content: 61.64

Gene sequence:

>1194_bases
ATGACCATGAACCAGTCGATCAACCCGCACGACCTTGGCGCCCATGCGGCCGCTGACGATCTCTTTACGCCTGTCGATCC
GGCGGCATTCAACGCCGTGTCTCCGCCGATCTTCCAGACATCGCTCTTCACTTACGACAGTTACGAGGCGATGGAGGATG
TCTTTGCCGGACGCACGCGCAACTATATCTATTCGCGCGGCGACAATCCGACGGTTCGCGAATTCGAACTGCTGGTCGCC
CGCCTCGAGGGCGCAGAGGACGGACGTGCCTTCTCCAGCGGAACGGCCGCCATTACATCGACAATTCTCAGTCTTGTTGA
GGCGGGAGACCGGGTCGTTGCGGTCCGCCATCTCTACAACGATGTCTACCGCCTTCTGGTGAAGCTGCTTGCCAGGCTCG
GTGTCGCGGTGGATTTCGTCGATCCCGCCGACCACGACGAGGTCCGCAAGGCCCTGTCGGGTGCCAAGCTGCTCTACCTC
GAAAACCCCACATCCTTCGTCTTCGAGCTTCAGGACATTGCGGCGCTGTCGGCCATGGCAAAGGAGGCTGGCGTCACGAG
TATCATCGACAATTCCTGGGCAACGCCGCTCTTTCAGAAACCGATCCAGCATGGCGTCGATATCGTCATTCACGCCGCCT
CGAAATATCTCGGCGGTCACAGCGATACGGTCGCCGGCGTCGTCGTCGGCTCGAAAGAAGCCATCGCCAGGATCAACGCG
ACATCCTACCCCTATGTCGGCGCCAAACTGTCACCGTTCGAAGCCTGGCTGCTGCTGCGCGGCATGCGCACGCTGCGTGT
CCGTCTCAAGGAGCATGAGCGCAGCGGGCTTTTGCTGGCCGGGCGGCTGAAGGAGCATTCTGCGATCGGATGCGTTCGTC
ACCCGGCATTTCAGGAGCATCCCGGGCGGGCGACGCTGACGGGCTATGCCGGGCTGTTTGCCTTCGACCTCAATCCCGAT
ATCGATGTCGCACGCTTTGTGAATAGCCTTCGCGACATCCGTCTCGGTGTCAGCTGGGGGGGACCCGAAACGCTGGTGGT
CCCCGCCAAGGTCGCGCTGCAGATCCCCGACCGGATGACCTCCTTCATCCGGTTCGGCGTGAGCGAGCAGACGGTTCGTT
TCGCCGTTGGCCTGGAAGAGCCGGAACTGCTGTGGAGCGATTTGCAGCAGGCGCTGCACGCAGCGAAACGGTAG

Upstream 100 bases:

>100_bases
TCAGCCGGCTGCCTGGCGAGGACGCTCAGCGCGCTCCGTCAGCGCGCTGCCGACCATCTGACCTCCATCCGTCCATATCC
CAACTTTTGTCCATGAGAGG

Downstream 100 bases:

>100_bases
GCTCCTGCGGGTGCGCATGGTCGGGGCGGGAAGCAGGTTCGGCGATCGCCAGATGGCAATGTTCCTCTGCCGCCTTGATC
ATGAAGTTGACGAGGGTCCT

Product: hypothetical protein

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTMNQSINPHDLGAHAAADDLFTPVDPAAFNAVSPPIFQTSLFTYDSYEAMEDVFAGRTRNYIYSRGDNPTVREFELLVA
RLEGAEDGRAFSSGTAAITSTILSLVEAGDRVVAVRHLYNDVYRLLVKLLARLGVAVDFVDPADHDEVRKALSGAKLLYL
ENPTSFVFELQDIAALSAMAKEAGVTSIIDNSWATPLFQKPIQHGVDIVIHAASKYLGGHSDTVAGVVVGSKEAIARINA
TSYPYVGAKLSPFEAWLLLRGMRTLRVRLKEHERSGLLLAGRLKEHSAIGCVRHPAFQEHPGRATLTGYAGLFAFDLNPD
IDVARFVNSLRDIRLGVSWGGPETLVVPAKVALQIPDRMTSFIRFGVSEQTVRFAVGLEEPELLWSDLQQALHAAKR

Sequences:

>Translated_397_residues
MTMNQSINPHDLGAHAAADDLFTPVDPAAFNAVSPPIFQTSLFTYDSYEAMEDVFAGRTRNYIYSRGDNPTVREFELLVA
RLEGAEDGRAFSSGTAAITSTILSLVEAGDRVVAVRHLYNDVYRLLVKLLARLGVAVDFVDPADHDEVRKALSGAKLLYL
ENPTSFVFELQDIAALSAMAKEAGVTSIIDNSWATPLFQKPIQHGVDIVIHAASKYLGGHSDTVAGVVVGSKEAIARINA
TSYPYVGAKLSPFEAWLLLRGMRTLRVRLKEHERSGLLLAGRLKEHSAIGCVRHPAFQEHPGRATLTGYAGLFAFDLNPD
IDVARFVNSLRDIRLGVSWGGPETLVVPAKVALQIPDRMTSFIRFGVSEQTVRFAVGLEEPELLWSDLQQALHAAKR
>Mature_396_residues
TMNQSINPHDLGAHAAADDLFTPVDPAAFNAVSPPIFQTSLFTYDSYEAMEDVFAGRTRNYIYSRGDNPTVREFELLVAR
LEGAEDGRAFSSGTAAITSTILSLVEAGDRVVAVRHLYNDVYRLLVKLLARLGVAVDFVDPADHDEVRKALSGAKLLYLE
NPTSFVFELQDIAALSAMAKEAGVTSIIDNSWATPLFQKPIQHGVDIVIHAASKYLGGHSDTVAGVVVGSKEAIARINAT
SYPYVGAKLSPFEAWLLLRGMRTLRVRLKEHERSGLLLAGRLKEHSAIGCVRHPAFQEHPGRATLTGYAGLFAFDLNPDI
DVARFVNSLRDIRLGVSWGGPETLVVPAKVALQIPDRMTSFIRFGVSEQTVRFAVGLEEPELLWSDLQQALHAAKR

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=397, Percent_Identity=30.9823677581864, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI25453487, Length=406, Percent_Identity=29.5566502463054, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI299473759, Length=397, Percent_Identity=27.7078085642317, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1790375, Length=378, Percent_Identity=30.1587301587302, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1789383, Length=374, Percent_Identity=31.283422459893, Blast_Score=165, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17560056, Length=382, Percent_Identity=31.413612565445, Blast_Score=184, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI17538055, Length=382, Percent_Identity=31.9371727748691, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI71980708, Length=374, Percent_Identity=28.0748663101604, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321170, Length=327, Percent_Identity=33.3333333333333, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6319307, Length=355, Percent_Identity=32.112676056338, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6323333, Length=410, Percent_Identity=25.3658536585366, Blast_Score=119, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6321254, Length=315, Percent_Identity=30.1587301587302, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321904, Length=374, Percent_Identity=26.2032085561497, Blast_Score=109, Evalue=8e-25,
Organism=Drosophila melanogaster, GI19922554, Length=378, Percent_Identity=30.1587301587302, Blast_Score=185, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 43411; Mature: 43280

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMNQSINPHDLGAHAAADDLFTPVDPAAFNAVSPPIFQTSLFTYDSYEAMEDVFAGRTR
CCCCCCCCCHHCCCHHHHHCCCCCCCCHHHCCCCCCHHHHEEECCCCHHHHHHHHCCCCC
NYIYSRGDNPTVREFELLVARLEGAEDGRAFSSGTAAITSTILSLVEAGDRVVAVRHLYN
CEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHH
DVYRLLVKLLARLGVAVDFVDPADHDEVRKALSGAKLLYLENPTSFVFELQDIAALSAMA
HHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHH
KEAGVTSIIDNSWATPLFQKPIQHGVDIVIHAASKYLGGHSDTVAGVVVGSKEAIARINA
HHCCCHHHHCCCCCCHHHHHHHHCCCEEEEEEHHHHCCCCCCCEEEEEECCHHHHHEECC
TSYPYVGAKLSPFEAWLLLRGMRTLRVRLKEHERSGLLLAGRLKEHSAIGCVRHPAFQEH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHCCCCCCCC
PGRATLTGYAGLFAFDLNPDIDVARFVNSLRDIRLGVSWGGPETLVVPAKVALQIPDRMT
CCCEEEEECCEEEEEECCCCCHHHHHHHHHHHHEEEEECCCCCEEEECCHHEEECCHHHH
SFIRFGVSEQTVRFAVGLEEPELLWSDLQQALHAAKR
HHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TMNQSINPHDLGAHAAADDLFTPVDPAAFNAVSPPIFQTSLFTYDSYEAMEDVFAGRTR
CCCCCCCCHHCCCHHHHHCCCCCCCCHHHCCCCCCHHHHEEECCCCHHHHHHHHCCCCC
NYIYSRGDNPTVREFELLVARLEGAEDGRAFSSGTAAITSTILSLVEAGDRVVAVRHLYN
CEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHH
DVYRLLVKLLARLGVAVDFVDPADHDEVRKALSGAKLLYLENPTSFVFELQDIAALSAMA
HHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHH
KEAGVTSIIDNSWATPLFQKPIQHGVDIVIHAASKYLGGHSDTVAGVVVGSKEAIARINA
HHCCCHHHHCCCCCCHHHHHHHHCCCEEEEEEHHHHCCCCCCCEEEEEECCHHHHHEECC
TSYPYVGAKLSPFEAWLLLRGMRTLRVRLKEHERSGLLLAGRLKEHSAIGCVRHPAFQEH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHCCCCCCCC
PGRATLTGYAGLFAFDLNPDIDVARFVNSLRDIRLGVSWGGPETLVVPAKVALQIPDRMT
CCCEEEEECCEEEEEECCCCCHHHHHHHHHHHHEEEEECCCCCEEEECCHHEEECCHHHH
SFIRFGVSEQTVRFAVGLEEPELLWSDLQQALHAAKR
HHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]