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The map label for this gene is ycaN [H]

Identifier: 116248963

GI number: 116248963

Start: 311189

End: 312091

Strand: Direct

Name: ycaN [H]

Synonym: pRL120294

Alternate gene names: 116248963

Gene position: 311189-312091 (Clockwise)

Preceding gene: 116248960

Following gene: 116248965

Centisome position: 35.77

GC content: 60.24

Gene sequence:

>903_bases
ATGGAACACGCCAGCTTGAAGGAACTCGAAGCGGCCATTGCGATTGCCCGGCGCGGGACATTCCGCGCAGCAGCCATCGA
TCTCGGCATGTCGACCACCGCGCTCAGCCACACCATAGGCAGGCTTGAGGCCGGGCTCGGCGTGCGACTGTTCAACCGCA
CCACACGCAGCGTGTCGCTGACTGATGCCGGCAGGCTGTTTGTGCAGCAGGTAGGGCCTACGCTTCAGGATCTCCATGCT
GCTCTGGATCTGGTACGCTCCCACCGCGAGACTCCTTCCGGAACAATACGGATCAACGCAGCGCCGTTTGCGGCGCGCGC
CATCATCTCACCGCTCGTGCTGGAGTTCCTGCGCCGCTATCCCGACATGAATGTCGATATCGTTACCGAGGGGAAAATGG
TCGATATCGTCAAGGAGGGGTTCGATCTGGGCGTCAGGGTTGAAGGCCTCGTTCCCACCGACATGATTGCCGTTTCGCTC
GGGCGGCCTCAGCGACACGCTGTCGTCGGGTCTCCTGAGTATTTCAAGAAGCACGGTAGGCCCATGGTGCCTCCGGATCT
TCTCAATCACCGATGCATCCGCGTGCGGCTGCCTGACGGCTCCTTGTTTCGATGGCGGTTCGAAAAAGACGGCGAGCCGG
TGCAGATAGACGTGCGGGGGCCAATCGCACTCGATGAGGCCAGCCTTACGCGGACTGCCGTGCTTGAAAACGCCGGCGTG
GGCTACCTCTTTGAACAAGACATTCTCTCGGACATAGAGGCGGGGCGCGTTGCTCGTGTTCTAGAAGATTGGACGCCGCC
CTATCCAGGCCTGTGCCTCTACTATCCTGGACGCCGAAATCTATCGGCTGGCGTCAGAGCATTTCTCGACTTAGCTCGTG
AGTTCTCCCGAAAAGCGGCTTAA

Upstream 100 bases:

>100_bases
CCATCCTGTTTCTCCTCATGATCTGCCCCGATAGTTAGCGCTCGACCATTGCATAGATAAGTGGGATAAACCGGAATTCG
GTATTGAACGGATCGGGACA

Downstream 100 bases:

>100_bases
CCCACTGCCTTCCGCCTTGAACGCTTTCGAACCGTTGGCCGGTGAACCCTCCTGCTTCGTTCCTGTCTACCGCCCGCCCT
GCTACTGCGCCGGCTCCAGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MEHASLKELEAAIAIARRGTFRAAAIDLGMSTTALSHTIGRLEAGLGVRLFNRTTRSVSLTDAGRLFVQQVGPTLQDLHA
ALDLVRSHRETPSGTIRINAAPFAARAIISPLVLEFLRRYPDMNVDIVTEGKMVDIVKEGFDLGVRVEGLVPTDMIAVSL
GRPQRHAVVGSPEYFKKHGRPMVPPDLLNHRCIRVRLPDGSLFRWRFEKDGEPVQIDVRGPIALDEASLTRTAVLENAGV
GYLFEQDILSDIEAGRVARVLEDWTPPYPGLCLYYPGRRNLSAGVRAFLDLAREFSRKAA

Sequences:

>Translated_300_residues
MEHASLKELEAAIAIARRGTFRAAAIDLGMSTTALSHTIGRLEAGLGVRLFNRTTRSVSLTDAGRLFVQQVGPTLQDLHA
ALDLVRSHRETPSGTIRINAAPFAARAIISPLVLEFLRRYPDMNVDIVTEGKMVDIVKEGFDLGVRVEGLVPTDMIAVSL
GRPQRHAVVGSPEYFKKHGRPMVPPDLLNHRCIRVRLPDGSLFRWRFEKDGEPVQIDVRGPIALDEASLTRTAVLENAGV
GYLFEQDILSDIEAGRVARVLEDWTPPYPGLCLYYPGRRNLSAGVRAFLDLAREFSRKAA
>Mature_300_residues
MEHASLKELEAAIAIARRGTFRAAAIDLGMSTTALSHTIGRLEAGLGVRLFNRTTRSVSLTDAGRLFVQQVGPTLQDLHA
ALDLVRSHRETPSGTIRINAAPFAARAIISPLVLEFLRRYPDMNVDIVTEGKMVDIVKEGFDLGVRVEGLVPTDMIAVSL
GRPQRHAVVGSPEYFKKHGRPMVPPDLLNHRCIRVRLPDGSLFRWRFEKDGEPVQIDVRGPIALDEASLTRTAVLENAGV
GYLFEQDILSDIEAGRVARVLEDWTPPYPGLCLYYPGRRNLSAGVRAFLDLAREFSRKAA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=44.2906574394464, Blast_Score=265, Evalue=2e-72,
Organism=Escherichia coli, GI1787589, Length=291, Percent_Identity=35.0515463917526, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1786401, Length=298, Percent_Identity=28.5234899328859, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI87081978, Length=290, Percent_Identity=28.6206896551724, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1789440, Length=258, Percent_Identity=27.5193798449612, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1789639, Length=274, Percent_Identity=27.3722627737226, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788706, Length=229, Percent_Identity=25.764192139738, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787601, Length=137, Percent_Identity=32.8467153284672, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=24.5674740484429, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI157672245, Length=136, Percent_Identity=33.8235294117647, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788748, Length=147, Percent_Identity=31.9727891156463, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1786508, Length=125, Percent_Identity=32.8, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33114; Mature: 33114

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHASLKELEAAIAIARRGTFRAAAIDLGMSTTALSHTIGRLEAGLGVRLFNRTTRSVSL
CCCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEE
TDAGRLFVQQVGPTLQDLHAALDLVRSHRETPSGTIRINAAPFAARAIISPLVLEFLRRY
CHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHC
PDMNVDIVTEGKMVDIVKEGFDLGVRVEGLVPTDMIAVSLGRPQRHAVVGSPEYFKKHGR
CCCCEEEEECCCCHHHHHCCCCCCEEEECCCCHHHEEEECCCCCCCEEECCHHHHHHCCC
PMVPPDLLNHRCIRVRLPDGSLFRWRFEKDGEPVQIDVRGPIALDEASLTRTAVLENAGV
CCCCHHHHCCCEEEEECCCCCEEEEEECCCCCEEEEEECCCEEECCHHHHHHHHHHCCCC
GYLFEQDILSDIEAGRVARVLEDWTPPYPGLCLYYPGRRNLSAGVRAFLDLAREFSRKAA
CEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEHASLKELEAAIAIARRGTFRAAAIDLGMSTTALSHTIGRLEAGLGVRLFNRTTRSVSL
CCCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEE
TDAGRLFVQQVGPTLQDLHAALDLVRSHRETPSGTIRINAAPFAARAIISPLVLEFLRRY
CHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHC
PDMNVDIVTEGKMVDIVKEGFDLGVRVEGLVPTDMIAVSLGRPQRHAVVGSPEYFKKHGR
CCCCEEEEECCCCHHHHHCCCCCCEEEECCCCHHHEEEECCCCCCCEEECCHHHHHHCCC
PMVPPDLLNHRCIRVRLPDGSLFRWRFEKDGEPVQIDVRGPIALDEASLTRTAVLENAGV
CCCCHHHHCCCEEEEECCCCCEEEEEECCCCCEEEEEECCCEEECCHHHHHHHHHHCCCC
GYLFEQDILSDIEAGRVARVLEDWTPPYPGLCLYYPGRRNLSAGVRAFLDLAREFSRKAA
CEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]