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The map label for this gene is melA [H]

Identifier: 116248926

GI number: 116248926

Start: 267534

End: 268850

Strand: Reverse

Name: melA [H]

Synonym: pRL120256

Alternate gene names: 116248926

Gene position: 268850-267534 (Counterclockwise)

Preceding gene: 116248928

Following gene: 116248925

Centisome position: 30.9

GC content: 57.18

Gene sequence:

>1317_bases
ATGCCGAAAATTTGCCTGGTAGGCGCTGGGAGCACCGTTTTTGCTCAGAACATTCTGGGAGATGTCCTGTCTTCGCAAAG
AGGCGGCGACTATCTCATCAGCCTCTTCGATATCGATCCAGAGCGACTGAAGACTTCAGAGATCGTTGCTCGCCGGATCT
GCGAGTCGCTTAAGCTTTCAAACGTCAGGATAGACGCCACGCTCGACCGGCGGGAAGCGCTGCGCGGTTCCGATTTCGTG
ATCCTCATGATGCAGGTCGGCGGCTACAAGCCGGCGACGGTCACGGACTTCGACATTCCAAAAAAATATGGCTTGCGTCA
GACGATCGCCGACACCCTTGGCATCGGCGGCATTTTCCGCGGTTTGCGGACGATACCAGTCCTTGAGGCGATCTGCCGAG
ACATGCAGGAGGTCTGCCCACAGGCTCTTCTGATGCAGTACGTCAACCCGATGGCCATCAACTGCTGGGCGATCAAGGAT
CTGGCCCCGGAAATCCGCACCGTCGGCTTGTGTCACAGTGTGCAGCACACAGCCGGGCATCTCGCGCAATGCCTCGGCGA
GGACATTGCCGACGTGAACTATGTTTCGGCTGGGATCAACCACGTCGCCTTCTTCCTGAAATATGAGAAAATTCATAGCG
ACGGACGGCGGGAAGACCTTTATCCGAGACTGAACGCTCTCGCCGCCGATGGCCGCGTTCCGCCGGACGATCGGGTGCGG
TTCGATGTTCTCAAACGGCTCGGCCACTTCGTCACCGAATCCAGCGAGCATTTCTCCGAGTATACCTCCTGGTACATCAA
GGAGGGACGAGGGGATCTGATCGATCAGCTCAATATTCCGTTGGACGAATACATCCGGCGCTGCGAGGTGCAGATCAAAG
AGTGGCATGCCCTTCGCAAGGAACTGGAAGGCGACAAGCCGATCGAGGTGTGCCGCAGCAATGAATATGCGGCCGGCATC
ATTCATGCCGCAATAACCGGCAACCCGGCGCTGATATACGGCAATGTCCCAAACAACGGCCTGATCGAGAATCTGCCCGA
TGAATGCATCGTGGAAGTGCCTTGCCATGTCGACAGAAACGGAATTCAGCCGGTCCGGGTCGGTCGGATCCCCTCTCAGC
TTTCCGCCGTCATGAACTTGAGCGTTTCCGTTCAGCAGTTGACGGTCGAGGCGGCTCTTACAAGAAACCGCGAGCGCATC
TACCAGGCCGCTCTGCTCGATCCGCATACGTCCGCGGAATTGTCGCCGGACCAAATCTGGAACCTTGTCGACGACCTGAT
CGTCGCACACGGCGATTTGTTGCCGAGATATCAGTGA

Upstream 100 bases:

>100_bases
TTTGCAGAAGACTCGAATCCCGGAATTAATAGGCCGATGCCAGGAGGAAATTCTGATCTAGAAGGAGCAACAAGCAATCA
CAACCCATGGGGAGATTGCA

Downstream 100 bases:

>100_bases
GACCCCGCGGATAAGCCAGCCACAAGCGCAGGGCAGTTCATTTGTTTAGACGCGTCGTGAATTCACGGCGCGTTTTTTTC
ATTCCAGCGTCATCCAATAA

Product: alpha-galactosidase (melibiase)

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MPKICLVGAGSTVFAQNILGDVLSSQRGGDYLISLFDIDPERLKTSEIVARRICESLKLSNVRIDATLDRREALRGSDFV
ILMMQVGGYKPATVTDFDIPKKYGLRQTIADTLGIGGIFRGLRTIPVLEAICRDMQEVCPQALLMQYVNPMAINCWAIKD
LAPEIRTVGLCHSVQHTAGHLAQCLGEDIADVNYVSAGINHVAFFLKYEKIHSDGRREDLYPRLNALAADGRVPPDDRVR
FDVLKRLGHFVTESSEHFSEYTSWYIKEGRGDLIDQLNIPLDEYIRRCEVQIKEWHALRKELEGDKPIEVCRSNEYAAGI
IHAAITGNPALIYGNVPNNGLIENLPDECIVEVPCHVDRNGIQPVRVGRIPSQLSAVMNLSVSVQQLTVEAALTRNRERI
YQAALLDPHTSAELSPDQIWNLVDDLIVAHGDLLPRYQ

Sequences:

>Translated_438_residues
MPKICLVGAGSTVFAQNILGDVLSSQRGGDYLISLFDIDPERLKTSEIVARRICESLKLSNVRIDATLDRREALRGSDFV
ILMMQVGGYKPATVTDFDIPKKYGLRQTIADTLGIGGIFRGLRTIPVLEAICRDMQEVCPQALLMQYVNPMAINCWAIKD
LAPEIRTVGLCHSVQHTAGHLAQCLGEDIADVNYVSAGINHVAFFLKYEKIHSDGRREDLYPRLNALAADGRVPPDDRVR
FDVLKRLGHFVTESSEHFSEYTSWYIKEGRGDLIDQLNIPLDEYIRRCEVQIKEWHALRKELEGDKPIEVCRSNEYAAGI
IHAAITGNPALIYGNVPNNGLIENLPDECIVEVPCHVDRNGIQPVRVGRIPSQLSAVMNLSVSVQQLTVEAALTRNRERI
YQAALLDPHTSAELSPDQIWNLVDDLIVAHGDLLPRYQ
>Mature_437_residues
PKICLVGAGSTVFAQNILGDVLSSQRGGDYLISLFDIDPERLKTSEIVARRICESLKLSNVRIDATLDRREALRGSDFVI
LMMQVGGYKPATVTDFDIPKKYGLRQTIADTLGIGGIFRGLRTIPVLEAICRDMQEVCPQALLMQYVNPMAINCWAIKDL
APEIRTVGLCHSVQHTAGHLAQCLGEDIADVNYVSAGINHVAFFLKYEKIHSDGRREDLYPRLNALAADGRVPPDDRVRF
DVLKRLGHFVTESSEHFSEYTSWYIKEGRGDLIDQLNIPLDEYIRRCEVQIKEWHALRKELEGDKPIEVCRSNEYAAGII
HAAITGNPALIYGNVPNNGLIENLPDECIVEVPCHVDRNGIQPVRVGRIPSQLSAVMNLSVSVQQLTVEAALTRNRERIY
QAALLDPHTSAELSPDQIWNLVDDLIVAHGDLLPRYQ

Specific function: Unknown

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=445, Percent_Identity=51.9101123595506, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1788029, Length=460, Percent_Identity=25, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 48915; Mature: 48784

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKICLVGAGSTVFAQNILGDVLSSQRGGDYLISLFDIDPERLKTSEIVARRICESLKLS
CCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHC
NVRIDATLDRREALRGSDFVILMMQVGGYKPATVTDFDIPKKYGLRQTIADTLGIGGIFR
CEEEEEEECHHHHCCCCCEEEEEEECCCCCCCEECCCCCCHHHCHHHHHHHHHCHHHHHH
GLRTIPVLEAICRDMQEVCPQALLMQYVNPMAINCWAIKDLAPEIRTVGLCHSVQHTAGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
LAQCLGEDIADVNYVSAGINHVAFFLKYEKIHSDGRREDLYPRLNALAADGRVPPDDRVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHH
FDVLKRLGHFVTESSEHFSEYTSWYIKEGRGDLIDQLNIPLDEYIRRCEVQIKEWHALRK
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ELEGDKPIEVCRSNEYAAGIIHAAITGNPALIYGNVPNNGLIENLPDECIVEVPCHVDRN
HCCCCCCHHHHCCCCCHHEEEEEEECCCCEEEECCCCCCCCCCCCCCHHEEECCCCCCCC
GIQPVRVGRIPSQLSAVMNLSVSVQQLTVEAALTRNRERIYQAALLDPHTSAELSPDQIW
CCCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHH
NLVDDLIVAHGDLLPRYQ
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PKICLVGAGSTVFAQNILGDVLSSQRGGDYLISLFDIDPERLKTSEIVARRICESLKLS
CCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHC
NVRIDATLDRREALRGSDFVILMMQVGGYKPATVTDFDIPKKYGLRQTIADTLGIGGIFR
CEEEEEEECHHHHCCCCCEEEEEEECCCCCCCEECCCCCCHHHCHHHHHHHHHCHHHHHH
GLRTIPVLEAICRDMQEVCPQALLMQYVNPMAINCWAIKDLAPEIRTVGLCHSVQHTAGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
LAQCLGEDIADVNYVSAGINHVAFFLKYEKIHSDGRREDLYPRLNALAADGRVPPDDRVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHH
FDVLKRLGHFVTESSEHFSEYTSWYIKEGRGDLIDQLNIPLDEYIRRCEVQIKEWHALRK
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ELEGDKPIEVCRSNEYAAGIIHAAITGNPALIYGNVPNNGLIENLPDECIVEVPCHVDRN
HCCCCCCHHHHCCCCCHHEEEEEEECCCCEEEECCCCCCCCCCCCCCHHEEECCCCCCCC
GIQPVRVGRIPSQLSAVMNLSVSVQQLTVEAALTRNRERIYQAALLDPHTSAELSPDQIW
CCCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHH
NLVDDLIVAHGDLLPRYQ
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3031590; 7610040; 9278503; 6329200; 2831880 [H]