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The map label for this gene is glmS [H]

Identifier: 116248920

GI number: 116248920

Start: 261571

End: 262524

Strand: Reverse

Name: glmS [H]

Synonym: pRL120250

Alternate gene names: 116248920

Gene position: 262524-261571 (Counterclockwise)

Preceding gene: 116248921

Following gene: 116248919

Centisome position: 30.17

GC content: 61.53

Gene sequence:

>954_bases
ATGAACACGACCGAAAAAGTCATTTTCGAGCAATTTCCCTACTGGGAGAAGGCGATCGGCTCGAATTCAGCTATCGATAG
CGCGGAGCTGCTCGTCTTCGTCGGCTGCGGCACGTCCTTCAATCTGGCGCTTGCGCTGGCATCCTATTCGAATATGGCCG
GACGCAAGGCAATCGCGGTTCCCGGTGCCGAATGGCAAAACCGGCCATCGGCCTTCTGGCCGGAGTGGCGCAACACGCAC
GTCGTCGCGCTTTCCCGGAGCGGCGAGACGACGGAGACAGTTGCCGCGGCAAAGGCAAGCCGAGCCGCCGGCGCCTTCGT
GACGGCGATAACCGTCGAACCGGAAAGCGCCCTCGCGAAAAATTGCGATCGGATGATCGCTGCCGAAACCCATCCGGACG
AAGGTATCGTTATGACGGTCTCGGCGAGCCTCATGGTCCTTCTCGGTTTGCAGATGATCGGGCAGAAAGTTCCTGCCTCT
GCCGTGAATTCCGCCCGCCAGCTCGCGAGCGCTCTCGATGCGGCGCTTCCTGGAATGATCGCCGACCGGTCGCACTTCGT
CTTTCTCGGTGGCGGGCCGCTCTTCGGCGTCGCCCTGGAGGGAGCGCTCAAGCTCATGGAAATGAGCCAGATCATGACCC
AGGCCTTCCACCCGCTGGAATACCGGCATGGGCCGATTAGCCTCGTTGACGCAAAGACGGTTGCGGTCATGCTGTATAGC
TCCGACCAGAGGGAGGCGGAGACGAAACTGGTGGGAGAACTGAGAGAAAAAGGCGCGGTCGTCATCGGTGTGGGTGGTCC
AGGCGATCTGGAACTCGCCGTCGATGTCGATCTGTCGCTTGCGGGTCTCGTTGTCCTTCCGGCGCTGCAGGTCCTCGGCG
AACGCGCCGCGCAGGCTCGGCAAATCGACACTGTCTCTCCCCGTCACCTGACGAAGGTTGTGACGCTCGCATGA

Upstream 100 bases:

>100_bases
GCTCGATCGCCATCGTCATCCCCGCCCTCGTCGTCATTTTCCTCAACCGTTACCTCGTCAGCGGCCTGTTGGCTGGTTCA
GTCAAATAATCGGAGACTTC

Downstream 100 bases:

>100_bases
CGTCGATCGAAGAGAACCGGCAGACCGCGCTGCGAAAGCTTTTCGGTGCCCGAAACCTGCCTTTTCCCCATGGCGTCACT
TCGGTCTGCTCGGCGCATCC

Product: putative glucosamine--fructose-6-phosphate aminotransferase (isomerizing)

Products: NA

Alternate protein names: D-fructose-6-phosphate amidotransferase; GFAT; Glucosamine-6-phosphate synthase; Hexosephosphate aminotransferase; L-glutamine-D-fructose-6-phosphate amidotransferase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MNTTEKVIFEQFPYWEKAIGSNSAIDSAELLVFVGCGTSFNLALALASYSNMAGRKAIAVPGAEWQNRPSAFWPEWRNTH
VVALSRSGETTETVAAAKASRAAGAFVTAITVEPESALAKNCDRMIAAETHPDEGIVMTVSASLMVLLGLQMIGQKVPAS
AVNSARQLASALDAALPGMIADRSHFVFLGGGPLFGVALEGALKLMEMSQIMTQAFHPLEYRHGPISLVDAKTVAVMLYS
SDQREAETKLVGELREKGAVVIGVGGPGDLELAVDVDLSLAGLVVLPALQVLGERAAQARQIDTVSPRHLTKVVTLA

Sequences:

>Translated_317_residues
MNTTEKVIFEQFPYWEKAIGSNSAIDSAELLVFVGCGTSFNLALALASYSNMAGRKAIAVPGAEWQNRPSAFWPEWRNTH
VVALSRSGETTETVAAAKASRAAGAFVTAITVEPESALAKNCDRMIAAETHPDEGIVMTVSASLMVLLGLQMIGQKVPAS
AVNSARQLASALDAALPGMIADRSHFVFLGGGPLFGVALEGALKLMEMSQIMTQAFHPLEYRHGPISLVDAKTVAVMLYS
SDQREAETKLVGELREKGAVVIGVGGPGDLELAVDVDLSLAGLVVLPALQVLGERAAQARQIDTVSPRHLTKVVTLA
>Mature_317_residues
MNTTEKVIFEQFPYWEKAIGSNSAIDSAELLVFVGCGTSFNLALALASYSNMAGRKAIAVPGAEWQNRPSAFWPEWRNTH
VVALSRSGETTETVAAAKASRAAGAFVTAITVEPESALAKNCDRMIAAETHPDEGIVMTVSASLMVLLGLQMIGQKVPAS
AVNSARQLASALDAALPGMIADRSHFVFLGGGPLFGVALEGALKLMEMSQIMTQAFHPLEYRHGPISLVDAKTVAVMLYS
SDQREAETKLVGELREKGAVVIGVGGPGDLELAVDVDLSLAGLVVLPALQVLGERAAQARQIDTVSPRHLTKVVTLA

Specific function: Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source [H]

COG id: COG2222

COG function: function code M; Predicted phosphosugar isomerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 SIS domains [H]

Homologues:

Organism=Escherichia coli, GI1790167, Length=343, Percent_Identity=26.2390670553936, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1789525, Length=249, Percent_Identity=27.710843373494, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17539970, Length=325, Percent_Identity=24.6153846153846, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17532899, Length=324, Percent_Identity=24.6913580246914, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17532897, Length=324, Percent_Identity=24.6913580246914, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6322745, Length=330, Percent_Identity=24.2424242424242, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR005855
- InterPro:   IPR001347 [H]

Pfam domain/function: PF00310 GATase_2; PF01380 SIS [H]

EC number: =2.6.1.16 [H]

Molecular weight: Translated: 33496; Mature: 33496

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTEKVIFEQFPYWEKAIGSNSAIDSAELLVFVGCGTSFNLALALASYSNMAGRKAIAV
CCCHHHHHHHHCCCHHHHCCCCCCCCCCEEEEEEECCCCCCHHEEEHHHHCCCCCEEEEC
PGAEWQNRPSAFWPEWRNTHVVALSRSGETTETVAAAKASRAAGAFVTAITVEPESALAK
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCHHHHHH
NCDRMIAAETHPDEGIVMTVSASLMVLLGLQMIGQKVPASAVNSARQLASALDAALPGMI
CHHHEEEECCCCCCCEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCE
ADRSHFVFLGGGPLFGVALEGALKLMEMSQIMTQAFHPLEYRHGPISLVDAKTVAVMLYS
ECCCCEEEEECCCEEHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEECCEEEEEEEC
SDQREAETKLVGELREKGAVVIGVGGPGDLELAVDVDLSLAGLVVLPALQVLGERAAQAR
CCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
QIDTVSPRHLTKVVTLA
CCCCCCHHHHHEEEECC
>Mature Secondary Structure
MNTTEKVIFEQFPYWEKAIGSNSAIDSAELLVFVGCGTSFNLALALASYSNMAGRKAIAV
CCCHHHHHHHHCCCHHHHCCCCCCCCCCEEEEEEECCCCCCHHEEEHHHHCCCCCEEEEC
PGAEWQNRPSAFWPEWRNTHVVALSRSGETTETVAAAKASRAAGAFVTAITVEPESALAK
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCHHHHHH
NCDRMIAAETHPDEGIVMTVSASLMVLLGLQMIGQKVPASAVNSARQLASALDAALPGMI
CHHHEEEECCCCCCCEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCE
ADRSHFVFLGGGPLFGVALEGALKLMEMSQIMTQAFHPLEYRHGPISLVDAKTVAVMLYS
ECCCCEEEEECCCEEHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEECCEEEEEEEC
SDQREAETKLVGELREKGAVVIGVGGPGDLELAVDVDLSLAGLVVLPALQVLGERAAQAR
CCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
QIDTVSPRHLTKVVTLA
CCCCCCHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]