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The map label for this gene is gatA2 [H]

Identifier: 116248810

GI number: 116248810

Start: 136751

End: 138124

Strand: Reverse

Name: gatA2 [H]

Synonym: pRL120136

Alternate gene names: 116248810

Gene position: 138124-136751 (Counterclockwise)

Preceding gene: 116248811

Following gene: 116248809

Centisome position: 15.88

GC content: 66.3

Gene sequence:

>1374_bases
ATGACCCTTTCTTACGGCTCCCTCTCGATCGCCCAGCTCTCCACCCTCATCCAGGGCGGCAGTGTCGATCCTGTCACGGT
GGCAGAGCAGGTCTTGGACGCGATCGAGAGCCATGACGACAAGGCGGTCTTCATCTCGTTGACGCGGGAGCGGGCAATGG
CGGAAGCGCATGCCTCGTCGAAGCGGCTGAATGACGGTCGTTCGCTTGGCTTGCTCGAAGGCATTCCGGTTGCCTGGAAG
GATCTCTTCGATGTTGCCGGCAGCGTCACGGCGGCGGGGTCCAGGCTGCTTCTACGTGATCAGGCACCGGCCGCAGTCGA
CGCCGCCGTCGTCTCCGCGCTGGCTTCGGCGGGTATGGTGGCGACGGGCCGGGTCAATATGAGCGAGTTCGCCTTTTCCG
GCCTTGGCATCAATCCGCATTTCGGCACGCCGAAAAACCCTCGCAGCACCGATGTCGCCCGCATTCCGGGCGGCTCGTCC
TCCGGTTCGGGCGTGGCGGTGGCATCAGCTCTCGTGCCGGTCTCGATCGGCTCCGACACAGGTGGTTCAGTGCGGATCCC
GGCGGCGCTGAACGGCATCGTCGGCTACAAGGCGACGCGCGGACGATACCCGATGACAGGCGTGTTCCCCCTTTCCGGCA
GCCTCGATTCGCTCGGGCCGCTCTGCCGTTCGGTGCAGGATGCCATCTGGATAGACGCCGCGATGCGCGGAGCCATGGCG
CCGACGGCCAGTCGCCGTCCGCTCGCCGGGCTGGAGGTCGTCATCCCCACCAACGTCGTCTTCGACGATGCCGAGCCGGG
TGTCGTTGCGGCCTTCGAAGCGGCGGTTGAGCGTTTGTCCGCCGCCGGCGTCATGTTCACGCGGCTGGAGATCCCGGCCT
TCGCGGAAATTCTGCAGTTGATGGCAAGCCACGGAGCCCTGGTGACGGCGGAGGCCTACTGGCTGCACAAGGCGCGGATC
ACCGGGCCTGATGCCGGACTGATCGACCCGCGTGTGGTCTCGCGCACCCGCTTCGGTGAGGAGATCACCCTTCCGCACTA
CCTCGACATACTCTCTGCCCGCAAGCGGCTGATCGAAGCGGTGGACCGGCTGGTCGGCGACCGCCTGATCGCCTTCCCGA
CGGTCGCGCATGTGGCACCGTTGCTCGAGCCGCTGAAGACCGATGACCAGCTTTTCGTGGCGACCAACGGCAAGACACTG
CGCAACACGCTGCTCGGCAATTTTCTGGACTGGTGCGGGGTGTCGATCCCTTGCGGGGCAGGCGACGCCGGCATGCCGGT
CGGCTTCCTGCTGTCGGGGCTGGCGCGCGGCGACGAGCGGCTGCTGTCGGTGGCGCTCGTCGCCGAACCGGTCATTCGCG
GCGAGACAATTTGA

Upstream 100 bases:

>100_bases
AACTCGATCGCCGTCTGGAACACGACCATGGACGAACACAATTATTTGGTGCGCCGCTGGATGCAGTTCGTGGCGGCGTG
AGCAGAACTGGAACGACAGA

Downstream 100 bases:

>100_bases
AGGCAGTCGCTCCGGTGGCTGCGAACATGAACAAGGAGAGTTGCAATGTGCATGGCGTGTTATGTCGACGAAGCGACCAA
GAGCAAGATCATCGCTTACA

Product: hypothetical protein

Products: NA

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTLSYGSLSIAQLSTLIQGGSVDPVTVAEQVLDAIESHDDKAVFISLTRERAMAEAHASSKRLNDGRSLGLLEGIPVAWK
DLFDVAGSVTAAGSRLLLRDQAPAAVDAAVVSALASAGMVATGRVNMSEFAFSGLGINPHFGTPKNPRSTDVARIPGGSS
SGSGVAVASALVPVSIGSDTGGSVRIPAALNGIVGYKATRGRYPMTGVFPLSGSLDSLGPLCRSVQDAIWIDAAMRGAMA
PTASRRPLAGLEVVIPTNVVFDDAEPGVVAAFEAAVERLSAAGVMFTRLEIPAFAEILQLMASHGALVTAEAYWLHKARI
TGPDAGLIDPRVVSRTRFGEEITLPHYLDILSARKRLIEAVDRLVGDRLIAFPTVAHVAPLLEPLKTDDQLFVATNGKTL
RNTLLGNFLDWCGVSIPCGAGDAGMPVGFLLSGLARGDERLLSVALVAEPVIRGETI

Sequences:

>Translated_457_residues
MTLSYGSLSIAQLSTLIQGGSVDPVTVAEQVLDAIESHDDKAVFISLTRERAMAEAHASSKRLNDGRSLGLLEGIPVAWK
DLFDVAGSVTAAGSRLLLRDQAPAAVDAAVVSALASAGMVATGRVNMSEFAFSGLGINPHFGTPKNPRSTDVARIPGGSS
SGSGVAVASALVPVSIGSDTGGSVRIPAALNGIVGYKATRGRYPMTGVFPLSGSLDSLGPLCRSVQDAIWIDAAMRGAMA
PTASRRPLAGLEVVIPTNVVFDDAEPGVVAAFEAAVERLSAAGVMFTRLEIPAFAEILQLMASHGALVTAEAYWLHKARI
TGPDAGLIDPRVVSRTRFGEEITLPHYLDILSARKRLIEAVDRLVGDRLIAFPTVAHVAPLLEPLKTDDQLFVATNGKTL
RNTLLGNFLDWCGVSIPCGAGDAGMPVGFLLSGLARGDERLLSVALVAEPVIRGETI
>Mature_456_residues
TLSYGSLSIAQLSTLIQGGSVDPVTVAEQVLDAIESHDDKAVFISLTRERAMAEAHASSKRLNDGRSLGLLEGIPVAWKD
LFDVAGSVTAAGSRLLLRDQAPAAVDAAVVSALASAGMVATGRVNMSEFAFSGLGINPHFGTPKNPRSTDVARIPGGSSS
GSGVAVASALVPVSIGSDTGGSVRIPAALNGIVGYKATRGRYPMTGVFPLSGSLDSLGPLCRSVQDAIWIDAAMRGAMAP
TASRRPLAGLEVVIPTNVVFDDAEPGVVAAFEAAVERLSAAGVMFTRLEIPAFAEILQLMASHGALVTAEAYWLHKARIT
GPDAGLIDPRVVSRTRFGEEITLPHYLDILSARKRLIEAVDRLVGDRLIAFPTVAHVAPLLEPLKTDDQLFVATNGKTLR
NTLLGNFLDWCGVSIPCGAGDAGMPVGFLLSGLARGDERLLSVALVAEPVIRGETI

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=505, Percent_Identity=25.1485148514851, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI195972892, Length=232, Percent_Identity=35.3448275862069, Blast_Score=113, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17543272, Length=470, Percent_Identity=27.6595744680851, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17537465, Length=520, Percent_Identity=24.6153846153846, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=214, Percent_Identity=30.3738317757009, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556264, Length=208, Percent_Identity=31.25, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=202, Percent_Identity=31.1881188118812, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=202, Percent_Identity=31.1881188118812, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=394, Percent_Identity=28.1725888324873, Blast_Score=104, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6323950, Length=344, Percent_Identity=27.0348837209302, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24648113, Length=251, Percent_Identity=35.8565737051793, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24652985, Length=411, Percent_Identity=25.0608272506083, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19922090, Length=411, Percent_Identity=25.0608272506083, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24652981, Length=411, Percent_Identity=25.0608272506083, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24652983, Length=411, Percent_Identity=25.0608272506083, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45550774, Length=185, Percent_Identity=31.8918918918919, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648435, Length=185, Percent_Identity=31.8918918918919, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648437, Length=185, Percent_Identity=31.8918918918919, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648441, Length=185, Percent_Identity=31.8918918918919, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648439, Length=185, Percent_Identity=31.8918918918919, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21356731, Length=184, Percent_Identity=32.6086956521739, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 47603; Mature: 47472

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSYGSLSIAQLSTLIQGGSVDPVTVAEQVLDAIESHDDKAVFISLTRERAMAEAHASS
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHH
KRLNDGRSLGLLEGIPVAWKDLFDVAGSVTAAGSRLLLRDQAPAAVDAAVVSALASAGMV
HHCCCCCCCCHHCCCCHHHHHHHHHHCCHHHCCCEEEEECCCCHHHHHHHHHHHHHCCEE
ATGRVNMSEFAFSGLGINPHFGTPKNPRSTDVARIPGGSSSGSGVAVASALVPVSIGSDT
EECCCCHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEHEEEEEEECCCC
GGSVRIPAALNGIVGYKATRGRYPMTGVFPLSGSLDSLGPLCRSVQDAIWIDAAMRGAMA
CCCEECCHHHCCHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
PTASRRPLAGLEVVIPTNVVFDDAEPGVVAAFEAAVERLSAAGVMFTRLEIPAFAEILQL
CCCCCCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHH
MASHGALVTAEAYWLHKARITGPDAGLIDPRVVSRTRFGEEITLPHYLDILSARKRLIEA
HHHCCCEEEEEEEEEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
VDRLVGDRLIAFPTVAHVAPLLEPLKTDDQLFVATNGKTLRNTLLGNFLDWCGVSIPCGA
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCC
GDAGMPVGFLLSGLARGDERLLSVALVAEPVIRGETI
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TLSYGSLSIAQLSTLIQGGSVDPVTVAEQVLDAIESHDDKAVFISLTRERAMAEAHASS
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHH
KRLNDGRSLGLLEGIPVAWKDLFDVAGSVTAAGSRLLLRDQAPAAVDAAVVSALASAGMV
HHCCCCCCCCHHCCCCHHHHHHHHHHCCHHHCCCEEEEECCCCHHHHHHHHHHHHHCCEE
ATGRVNMSEFAFSGLGINPHFGTPKNPRSTDVARIPGGSSSGSGVAVASALVPVSIGSDT
EECCCCHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEHEEEEEEECCCC
GGSVRIPAALNGIVGYKATRGRYPMTGVFPLSGSLDSLGPLCRSVQDAIWIDAAMRGAMA
CCCEECCHHHCCHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
PTASRRPLAGLEVVIPTNVVFDDAEPGVVAAFEAAVERLSAAGVMFTRLEIPAFAEILQL
CCCCCCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHH
MASHGALVTAEAYWLHKARITGPDAGLIDPRVVSRTRFGEEITLPHYLDILSARKRLIEA
HHHCCCEEEEEEEEEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
VDRLVGDRLIAFPTVAHVAPLLEPLKTDDQLFVATNGKTLRNTLLGNFLDWCGVSIPCGA
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCC
GDAGMPVGFLLSGLARGDERLLSVALVAEPVIRGETI
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]