Click here to switch to the map view.

The map label for this gene is hyuA [H]

Identifier: 116248800

GI number: 116248800

Start: 124801

End: 126234

Strand: Reverse

Name: hyuA [H]

Synonym: pRL120126

Alternate gene names: 116248800

Gene position: 126234-124801 (Counterclockwise)

Preceding gene: 116248801

Following gene: 116248799

Centisome position: 14.51

GC content: 61.3

Gene sequence:

>1434_bases
ATGACGCTCGCTGAGCCTTATGACCTCGTGATCCGTCGGGGCCGTGTGGTGCTGCCGGATGCAACCAAGCAAATCGATAT
CGGCGTTCGCGACGGCGTGATTGCGGCGCTCGGGCCGGATCTGCCCGAGGGGAAGCATGAAGTCGCGGCCGAGGGGCGTA
TCGTGCTGCCCGGCGGCGTCGACAGCCATTGCCACATGGATCAGCAGCCATGGGAAGGGAAGGCGACAGCGGACGATTTC
AATACCGGCACGCTGTCGGCGATGTGCGGTGGCACGACAACTGTGGTGCCTTTCGCCATGCAGATGCGTGGCCAGTCGCT
ACGCGACATCGTCGAGGACTATCACGAGCGCGCCCGTTCGAAAGCGCATATCGACTATGGTTTTCACCTCATCGTCGGCG
ATCCCTCAGCCGAAGTGTTGCGCGACGAAATTCCTCGGCTGATTGCCGAAGGCTGCACCTCAATCAAGATTTACCTCACC
TATGACGGTTTGAAGCTCGATGACTATGAGGTGCTGAATGTGCTCGACCTTGCCCGCGCGCAGGGCGCGATGGTCATGGT
TCACGCGGAAAACGACGCCTGCATTCGCTGGCTGACCGAAAAATTCATTGCCGCGCGCAAGACCGAATTGCGCTACCATG
AAAAGGCGCATTCAGCGATCGGCGACCGTGAAGCGACCTTCCGGGCGATCAGCCTTTCGGAACTGATAGAGACGCCGATC
CTCGTCAGCCACGTCGCTGCGGGCGGTGCCGTCGAGGAGATCCGCCGCGCCAAGGCGCGTGGGCTTCCGATCTACGCCGA
GACGTGCCCCCAATATCTTTTCCTTTCGGCCGAAGATATCGACACCCATGATCTCTCCGGCTCCAAATGCGTCTGCACGC
CGCCGCCACGCGATAAGTCGAACCAGCCAGCAATCTGGGCCGGCATCCTGGACGGAACGCTGGAGGTCTTTTCTTCGGAT
CATTCGCCCTGGCACTATGCCGACAAGATTGCGGGCGGGCCGGGGACGCCGTTCCACCGAATTCCCAATGGCATTCCCGG
CATCGAGACGCGACTTGCCCTTTTGTTTTCGGCAGGCGTGAATGGTGGCTTGATCTCGCTGCAGAAATTCGCGGACCTCA
CGTCCGGCGCGCCGGCACGCCTTTTCGGGCTTGCTCCGCGCAAGGGGCAGATTGCCGTCGGGGCAGACGCCGATATTGCG
ATCTGGGATCCGGATCGCAGCGTGACGATCACTAATTCCCTGCTGCACCACGCCACCGACTACACGCCCTACGAGGGGCA
GGTCGTCAAGGGCTGGCCCATCATGACGATCTCGCGCGGCGATATCGTCTGGAAGGACGGAAAAATCCTGGCGGAACCGG
GCCGCGGCCAATTCATCGCCAGAGAGCGGCCTTTTCCGCCGCAGCAGGGACTTTCTAAGGTCCTCGCATCATGA

Upstream 100 bases:

>100_bases
TCGGGAGCGGAGCGTTCCTGGTTGCTGCCACTGCCTGCTTCAATCTCCTGCGCCGTCCGATCGGCGCCGCCAAGCCTTGC
TTCACTACGGGAGGAAACGA

Downstream 100 bases:

>100_bases
CGACGCCGATGTCACGTCTTGAAGGCAAGGTGATTTTCCTGACCGGTGCAGCAAGCGGCATCGGCGCGGCGATCGCGCGC
CGATGCATCGACGAGAAAGC

Product: putative D-hydantoinase

Products: 3-ureidopropanoate

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MTLAEPYDLVIRRGRVVLPDATKQIDIGVRDGVIAALGPDLPEGKHEVAAEGRIVLPGGVDSHCHMDQQPWEGKATADDF
NTGTLSAMCGGTTTVVPFAMQMRGQSLRDIVEDYHERARSKAHIDYGFHLIVGDPSAEVLRDEIPRLIAEGCTSIKIYLT
YDGLKLDDYEVLNVLDLARAQGAMVMVHAENDACIRWLTEKFIAARKTELRYHEKAHSAIGDREATFRAISLSELIETPI
LVSHVAAGGAVEEIRRAKARGLPIYAETCPQYLFLSAEDIDTHDLSGSKCVCTPPPRDKSNQPAIWAGILDGTLEVFSSD
HSPWHYADKIAGGPGTPFHRIPNGIPGIETRLALLFSAGVNGGLISLQKFADLTSGAPARLFGLAPRKGQIAVGADADIA
IWDPDRSVTITNSLLHHATDYTPYEGQVVKGWPIMTISRGDIVWKDGKILAEPGRGQFIARERPFPPQQGLSKVLAS

Sequences:

>Translated_477_residues
MTLAEPYDLVIRRGRVVLPDATKQIDIGVRDGVIAALGPDLPEGKHEVAAEGRIVLPGGVDSHCHMDQQPWEGKATADDF
NTGTLSAMCGGTTTVVPFAMQMRGQSLRDIVEDYHERARSKAHIDYGFHLIVGDPSAEVLRDEIPRLIAEGCTSIKIYLT
YDGLKLDDYEVLNVLDLARAQGAMVMVHAENDACIRWLTEKFIAARKTELRYHEKAHSAIGDREATFRAISLSELIETPI
LVSHVAAGGAVEEIRRAKARGLPIYAETCPQYLFLSAEDIDTHDLSGSKCVCTPPPRDKSNQPAIWAGILDGTLEVFSSD
HSPWHYADKIAGGPGTPFHRIPNGIPGIETRLALLFSAGVNGGLISLQKFADLTSGAPARLFGLAPRKGQIAVGADADIA
IWDPDRSVTITNSLLHHATDYTPYEGQVVKGWPIMTISRGDIVWKDGKILAEPGRGQFIARERPFPPQQGLSKVLAS
>Mature_476_residues
TLAEPYDLVIRRGRVVLPDATKQIDIGVRDGVIAALGPDLPEGKHEVAAEGRIVLPGGVDSHCHMDQQPWEGKATADDFN
TGTLSAMCGGTTTVVPFAMQMRGQSLRDIVEDYHERARSKAHIDYGFHLIVGDPSAEVLRDEIPRLIAEGCTSIKIYLTY
DGLKLDDYEVLNVLDLARAQGAMVMVHAENDACIRWLTEKFIAARKTELRYHEKAHSAIGDREATFRAISLSELIETPIL
VSHVAAGGAVEEIRRAKARGLPIYAETCPQYLFLSAEDIDTHDLSGSKCVCTPPPRDKSNQPAIWAGILDGTLEVFSSDH
SPWHYADKIAGGPGTPFHRIPNGIPGIETRLALLFSAGVNGGLISLQKFADLTSGAPARLFGLAPRKGQIAVGADADIAI
WDPDRSVTITNSLLHHATDYTPYEGQVVKGWPIMTISRGDIVWKDGKILAEPGRGQFIARERPFPPQQGLSKVLAS

Specific function: Catalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin derivatives [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503375, Length=473, Percent_Identity=34.6723044397463, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI4503051, Length=464, Percent_Identity=32.1120689655172, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI62422571, Length=464, Percent_Identity=32.1120689655172, Blast_Score=239, Evalue=6e-63,
Organism=Homo sapiens, GI190194363, Length=464, Percent_Identity=31.6810344827586, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI4503377, Length=464, Percent_Identity=31.4655172413793, Blast_Score=224, Evalue=2e-58,
Organism=Homo sapiens, GI19923821, Length=464, Percent_Identity=31.25, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI4503379, Length=457, Percent_Identity=30.1969365426696, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI18105007, Length=442, Percent_Identity=26.9230769230769, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI87082175, Length=465, Percent_Identity=40.2150537634409, Blast_Score=321, Evalue=5e-89,
Organism=Escherichia coli, GI1786722, Length=471, Percent_Identity=29.9363057324841, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17539558, Length=458, Percent_Identity=32.9694323144105, Blast_Score=235, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI71989490, Length=465, Percent_Identity=32.4731182795699, Blast_Score=221, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI86575075, Length=464, Percent_Identity=26.0775862068966, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI193204318, Length=408, Percent_Identity=27.4509803921569, Blast_Score=108, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6322218, Length=425, Percent_Identity=28, Blast_Score=139, Evalue=9e-34,
Organism=Drosophila melanogaster, GI17137462, Length=458, Percent_Identity=32.5327510917031, Blast_Score=235, Evalue=5e-62,
Organism=Drosophila melanogaster, GI221377917, Length=470, Percent_Identity=33.1914893617021, Blast_Score=231, Evalue=9e-61,
Organism=Drosophila melanogaster, GI24642586, Length=415, Percent_Identity=28.9156626506024, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24644287, Length=320, Percent_Identity=30.9375, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24644289, Length=262, Percent_Identity=30.9160305343511, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI18859883, Length=366, Percent_Identity=27.0491803278689, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011778
- InterPro:   IPR011059
- InterPro:   IPR011612 [H]

Pfam domain/function: PF01979 Amidohydro_1; PF00449 Urease_alpha [H]

EC number: 3.5.2.2

Molecular weight: Translated: 51872; Mature: 51740

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAEPYDLVIRRGRVVLPDATKQIDIGVRDGVIAALGPDLPEGKHEVAAEGRIVLPGGV
CCCCCCHHHHHHCCEEECCCCCCEEECCCCCCEEEECCCCCCCCCHHHHCCCEEEECCCC
DSHCHMDQQPWEGKATADDFNTGTLSAMCGGTTTVVPFAMQMRGQSLRDIVEDYHERARS
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHC
KAHIDYGFHLIVGDPSAEVLRDEIPRLIAEGCTSIKIYLTYDGLKLDDYEVLNVLDLARA
CCEEEECEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEECCEEECCHHHHHHHHHHHC
QGAMVMVHAENDACIRWLTEKFIAARKTELRYHEKAHSAIGDREATFRAISLSELIETPI
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEHHHHHCCCH
LVSHVAAGGAVEEIRRAKARGLPIYAETCPQYLFLSAEDIDTHDLSGSKCVCTPPPRDKS
HHHHHHCCCHHHHHHHHHHCCCCEEHHCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCC
NQPAIWAGILDGTLEVFSSDHSPWHYADKIAGGPGTPFHRIPNGIPGIETRLALLFSAGV
CCCEEEEEEHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHCCC
NGGLISLQKFADLTSGAPARLFGLAPRKGQIAVGADADIAIWDPDRSVTITNSLLHHATD
CCCEEHHHHHHHHCCCCCHHEEECCCCCCCEEECCCCEEEEECCCCCEEEHHHHHHHHCC
YTPYEGQVVKGWPIMTISRGDIVWKDGKILAEPGRGQFIARERPFPPQQGLSKVLAS
CCCCCCEEEECCEEEEEECCCEEEECCEEEECCCCCCEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TLAEPYDLVIRRGRVVLPDATKQIDIGVRDGVIAALGPDLPEGKHEVAAEGRIVLPGGV
CCCCCHHHHHHCCEEECCCCCCEEECCCCCCEEEECCCCCCCCCHHHHCCCEEEECCCC
DSHCHMDQQPWEGKATADDFNTGTLSAMCGGTTTVVPFAMQMRGQSLRDIVEDYHERARS
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHC
KAHIDYGFHLIVGDPSAEVLRDEIPRLIAEGCTSIKIYLTYDGLKLDDYEVLNVLDLARA
CCEEEECEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEECCEEECCHHHHHHHHHHHC
QGAMVMVHAENDACIRWLTEKFIAARKTELRYHEKAHSAIGDREATFRAISLSELIETPI
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEHHHHHCCCH
LVSHVAAGGAVEEIRRAKARGLPIYAETCPQYLFLSAEDIDTHDLSGSKCVCTPPPRDKS
HHHHHHCCCHHHHHHHHHHCCCCEEHHCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCC
NQPAIWAGILDGTLEVFSSDHSPWHYADKIAGGPGTPFHRIPNGIPGIETRLALLFSAGV
CCCEEEEEEHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHCCC
NGGLISLQKFADLTSGAPARLFGLAPRKGQIAVGADADIAIWDPDRSVTITNSLLHHATD
CCCEEHHHHHHHHCCCCCHHEEECCCCCCCEEECCCCEEEEECCCCCEEEHHHHHHHHCC
YTPYEGQVVKGWPIMTISRGDIVWKDGKILAEPGRGQFIARERPFPPQQGLSKVLAS
CCCCCCEEEECCEEEEEECCCEEEECCEEEECCCCCCEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,6-dihydrouracil; H2O

Specific reaction: 5,6-dihydrouracil + H2O = 3-ureidopropanoate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA