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The map label for this gene is galE [C]

Identifier: 116248781

GI number: 116248781

Start: 100563

End: 101459

Strand: Direct

Name: galE [C]

Synonym: pRL120105

Alternate gene names: 116248781

Gene position: 100563-101459 (Clockwise)

Preceding gene: 116248780

Following gene: 116248782

Centisome position: 11.56

GC content: 60.09

Gene sequence:

>897_bases
ATGAGCAAGCGGATCATCTTTACCGGCGGCAGCGGCAAGGCCGGCCGCCATGCGGTTCCATATCTGGTGGAGAAGGGACA
CAAGGTCCGCAACCTCGACCTCGTTCCCTTGAACTGCCCCGGCGTGCAGACGTTGATCACCGATCTCAGCGATAGCGGCC
AGACCTTCAATGCCCTGTCGATGCATTTCAGCGGCGAGGGTCTGCAAACGCCGGGCGGACCGGCCAAGGTCGACGCCGTC
GTGCATTTCGCCGCTATACCGAGCCTGCTGCTCAAGCCCGACAACCAGACTTTTTCGGTCAATGCGATCTCGACCTACAA
CGTCATCGAAGCGGCGGTGAAGCTCGGCATTCCCAAGGTGATCATCGCGTCGAGCGAAACGACCTACGGCGTCTGTTTTG
CCGAGGGAGACAAGGATTTCCATTCGTTTCCGCTCGAAGAAGACTATGACATCGACCCGATGGACAGCTATGGGCTTTCC
AAGGTCGTCAACGAGAAGACGGCCCGCGCCTTTGCGATGCGCAGCGGCATCGACATCTACGCCCTGCGGATCGCCAACGT
CATCGAACCGCATGAATACGAGCGTTTTCCGGGTTTCCTTGCCGATCCGCCATCGCGCAAGCGCAACGCCTGGAGCTACA
TCGATGCCCGCGATCTCGGGCAGATCGTCGATCTTTGCCTGCGGGCCGACGGCCTCGGTTTCCAGGTCTTCAATGCCGTC
AACGACACGATCACGGCAAGCGAGCCAACGCGGGGTTTCCTCGGCAAATGGGCGCCGAACACGCCGATCCTCCGGGAGCT
CGGCGAATTCGAAGCGCCGCTCTCGAACCGGAAGATCCGGGAGGTTCTCGGTTTCAAGGAAGAACACAACTGGCGGAAAT
ACGTTTCCGGCGCATGA

Upstream 100 bases:

>100_bases
AGCCTGGTTCATCGGTTCAGTGATTTCCACTCTATTGATTCATCGCTCTCCCGCGTTGTACCGCTTCCATCGAACCTTCA
TCAGGAAGCGGACACATCAC

Downstream 100 bases:

>100_bases
TGCAGCGAGCCGCATTTGCGGCACCGCCGATCAACAACTCGGAGGAAAGACCATGAAGACCACAAGACTTGGCAAGACAG
GGCTTGAAGTCAGCCGCATC

Product: putative UDP-glucose 4-epimerase

Products: UDPglucoseal [C]

Alternate protein names: UDP-Glucose 4-Epimerase; UDP-Galactose 4-Epimerase; Nucleoside-Diphosphate-Sugar Epimerase; DTDP-Glucose 4 6-Dehydratase; NAD Dependent Epimerase/Dehydratase Family; Oxidoreductase Protein; UDP-Glucose-4-Epimerase; Nucleoside-Diphosphate-Sugar Epimerases; Epimerase/Dehydratase; Dehydratase/Oxidoreductase; Vegetative Cell Wall; P Nucleoside-Diphosphate-Sugar Epimerase Protein

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSKRIIFTGGSGKAGRHAVPYLVEKGHKVRNLDLVPLNCPGVQTLITDLSDSGQTFNALSMHFSGEGLQTPGGPAKVDAV
VHFAAIPSLLLKPDNQTFSVNAISTYNVIEAAVKLGIPKVIIASSETTYGVCFAEGDKDFHSFPLEEDYDIDPMDSYGLS
KVVNEKTARAFAMRSGIDIYALRIANVIEPHEYERFPGFLADPPSRKRNAWSYIDARDLGQIVDLCLRADGLGFQVFNAV
NDTITASEPTRGFLGKWAPNTPILRELGEFEAPLSNRKIREVLGFKEEHNWRKYVSGA

Sequences:

>Translated_298_residues
MSKRIIFTGGSGKAGRHAVPYLVEKGHKVRNLDLVPLNCPGVQTLITDLSDSGQTFNALSMHFSGEGLQTPGGPAKVDAV
VHFAAIPSLLLKPDNQTFSVNAISTYNVIEAAVKLGIPKVIIASSETTYGVCFAEGDKDFHSFPLEEDYDIDPMDSYGLS
KVVNEKTARAFAMRSGIDIYALRIANVIEPHEYERFPGFLADPPSRKRNAWSYIDARDLGQIVDLCLRADGLGFQVFNAV
NDTITASEPTRGFLGKWAPNTPILRELGEFEAPLSNRKIREVLGFKEEHNWRKYVSGA
>Mature_297_residues
SKRIIFTGGSGKAGRHAVPYLVEKGHKVRNLDLVPLNCPGVQTLITDLSDSGQTFNALSMHFSGEGLQTPGGPAKVDAVV
HFAAIPSLLLKPDNQTFSVNAISTYNVIEAAVKLGIPKVIIASSETTYGVCFAEGDKDFHSFPLEEDYDIDPMDSYGLSK
VVNEKTARAFAMRSGIDIYALRIANVIEPHEYERFPGFLADPPSRKRNAWSYIDARDLGQIVDLCLRADGLGFQVFNAVN
DTITASEPTRGFLGKWAPNTPILRELGEFEAPLSNRKIREVLGFKEEHNWRKYVSGA

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 32747; Mature: 32615

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRIIFTGGSGKAGRHAVPYLVEKGHKVRNLDLVPLNCPGVQTLITDLSDSGQTFNALS
CCCEEEEECCCCCCCCCCCCHHHHCCCEEECEEEEECCCCCHHHHHHHHCCCCCCEEEEE
MHFSGEGLQTPGGPAKVDAVVHFAAIPSLLLKPDNQTFSVNAISTYNVIEAAVKLGIPKV
EEECCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCEEEEEEEHHHHHHHHHHHHCCCEE
IIASSETTYGVCFAEGDKDFHSFPLEEDYDIDPMDSYGLSKVVNEKTARAFAMRSGIDIY
EEECCCCEEEEEEECCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
ALRIANVIEPHEYERFPGFLADPPSRKRNAWSYIDARDLGQIVDLCLRADGLGFQVFNAV
EEEHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHC
NDTITASEPTRGFLGKWAPNTPILRELGEFEAPLSNRKIREVLGFKEEHNWRKYVSGA
CCCEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHCCCC
>Mature Secondary Structure 
SKRIIFTGGSGKAGRHAVPYLVEKGHKVRNLDLVPLNCPGVQTLITDLSDSGQTFNALS
CCEEEEECCCCCCCCCCCCHHHHCCCEEECEEEEECCCCCHHHHHHHHCCCCCCEEEEE
MHFSGEGLQTPGGPAKVDAVVHFAAIPSLLLKPDNQTFSVNAISTYNVIEAAVKLGIPKV
EEECCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCEEEEEEEHHHHHHHHHHHHCCCEE
IIASSETTYGVCFAEGDKDFHSFPLEEDYDIDPMDSYGLSKVVNEKTARAFAMRSGIDIY
EEECCCCEEEEEEECCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
ALRIANVIEPHEYERFPGFLADPPSRKRNAWSYIDARDLGQIVDLCLRADGLGFQVFNAV
EEEHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHC
NDTITASEPTRGFLGKWAPNTPILRELGEFEAPLSNRKIREVLGFKEEHNWRKYVSGA
CCCEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA