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The map label for this gene is ycaN [H]

Identifier: 116248780

GI number: 116248780

Start: 99541

End: 100443

Strand: Direct

Name: ycaN [H]

Synonym: pRL120104

Alternate gene names: 116248780

Gene position: 99541-100443 (Clockwise)

Preceding gene: 116248778

Following gene: 116248781

Centisome position: 11.44

GC content: 58.47

Gene sequence:

>903_bases
ATGCAGTTCAAGCGCAATGAAATTCACGAACTGTCGATCTTTCTCGCGATAGTCCAGTACCGTAGCTTTCGGAAAGCGGC
GGATCACCTGGAAGTAACGGCTTCCGCCCTGAGCCACAGCATGAAGGCGCTTGAGCAAAGGCTGGGCGTTCGCCTCCTTA
ATCGAACCAGCCGCAGCGTTGCACCAACCGCCGCCGGAACAGCCCTCGCCGAGAAGATTTCTGCCGGGCTCGAGCTTATC
AACTCCGGATTGGAGGACTTGAACGGACATTATCAAGGTGGCGCCGGCAGCATTCGCATCAATGTTCTGAAAGATGCCGC
CGTTCTGCTTTTGCGGCCAGCCATCCCGGTGTTCCAGCAGCGCTTCCCGAATGTTGAACTCGAGGTCGCCGTGGATGACC
AGTTCGTCGATGTCACCGCGGACGGCTTCGATGCCGGTATCCGGTACAGCGGCACCATCCCGGAAGACATGATCGCAGTT
CCTCTCACTCCTCCTTTAAAGTGGGTCGCGGTCGCTGCCCCTGATTATCTCAGAAGTCATGGTCGCCCTGTCATGCCGGA
GGACCTCAATTCCCACCACTGCATCCGGATACGGACGGGCCGCGGCCAGATCTACAAGTGGGAATTCGAACGCGGCGATG
ACCGGCGCGAGATCGACGTTCCCGGAGCGCTGATATCAGGCGAAACCGATCTTGCCATCAATGCGGCTCTGGAAGGCGCC
GGGCTGTGCTATTGCCTGGAACGGTTGGCAAGCCCCTACGTTTCCGCAGGCCGTCTGGAAGTCGTTCTTCCCAAATGGGC
ATCGATAGGCCCGCCGTTTTCGATGTATTATTCCAGCCGGCGGCAGCTTCCCTTCGGCGTCGACGCGCTGATCAAGATCG
TTCGCGGCTTGAACGGATTTTAG

Upstream 100 bases:

>100_bases
CGAGCCGGTAAACCAGCATCCGAGTGCTGAGTTGCACCGAACGAGCTAGAGCCTTTAAGACCGGTTGCGAAGTATCGCCA
ACTAGCCTCCTGAGATCAAC

Downstream 100 bases:

>100_bases
CGGCTGGTTCCAATCGATGAGCCTGGTTCATCGGTTCAGTGATTTCCACTCTATTGATTCATCGCTCTCCCGCGTTGTAC
CGCTTCCATCGAACCTTCAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MQFKRNEIHELSIFLAIVQYRSFRKAADHLEVTASALSHSMKALEQRLGVRLLNRTSRSVAPTAAGTALAEKISAGLELI
NSGLEDLNGHYQGGAGSIRINVLKDAAVLLLRPAIPVFQQRFPNVELEVAVDDQFVDVTADGFDAGIRYSGTIPEDMIAV
PLTPPLKWVAVAAPDYLRSHGRPVMPEDLNSHHCIRIRTGRGQIYKWEFERGDDRREIDVPGALISGETDLAINAALEGA
GLCYCLERLASPYVSAGRLEVVLPKWASIGPPFSMYYSSRRQLPFGVDALIKIVRGLNGF

Sequences:

>Translated_300_residues
MQFKRNEIHELSIFLAIVQYRSFRKAADHLEVTASALSHSMKALEQRLGVRLLNRTSRSVAPTAAGTALAEKISAGLELI
NSGLEDLNGHYQGGAGSIRINVLKDAAVLLLRPAIPVFQQRFPNVELEVAVDDQFVDVTADGFDAGIRYSGTIPEDMIAV
PLTPPLKWVAVAAPDYLRSHGRPVMPEDLNSHHCIRIRTGRGQIYKWEFERGDDRREIDVPGALISGETDLAINAALEGA
GLCYCLERLASPYVSAGRLEVVLPKWASIGPPFSMYYSSRRQLPFGVDALIKIVRGLNGF
>Mature_300_residues
MQFKRNEIHELSIFLAIVQYRSFRKAADHLEVTASALSHSMKALEQRLGVRLLNRTSRSVAPTAAGTALAEKISAGLELI
NSGLEDLNGHYQGGAGSIRINVLKDAAVLLLRPAIPVFQQRFPNVELEVAVDDQFVDVTADGFDAGIRYSGTIPEDMIAV
PLTPPLKWVAVAAPDYLRSHGRPVMPEDLNSHHCIRIRTGRGQIYKWEFERGDDRREIDVPGALISGETDLAINAALEGA
GLCYCLERLASPYVSAGRLEVVLPKWASIGPPFSMYYSSRRQLPFGVDALIKIVRGLNGF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=36.426116838488, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1787589, Length=281, Percent_Identity=33.4519572953737, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI145693193, Length=299, Percent_Identity=31.7725752508361, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1786401, Length=284, Percent_Identity=27.112676056338, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789440, Length=267, Percent_Identity=28.0898876404494, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789639, Length=285, Percent_Identity=25.2631578947368, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87081978, Length=271, Percent_Identity=25.830258302583, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789173, Length=258, Percent_Identity=26.7441860465116, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788706, Length=262, Percent_Identity=23.6641221374046, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788748, Length=120, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786448, Length=238, Percent_Identity=25.6302521008403, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787879, Length=155, Percent_Identity=29.0322580645161, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790399, Length=213, Percent_Identity=28.169014084507, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI157672245, Length=155, Percent_Identity=28.3870967741935, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32962; Mature: 32962

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFKRNEIHELSIFLAIVQYRSFRKAADHLEVTASALSHSMKALEQRLGVRLLNRTSRSV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
APTAAGTALAEKISAGLELINSGLEDLNGHYQGGAGSIRINVLKDAAVLLLRPAIPVFQQ
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHCCCCHHHHH
RFPNVELEVAVDDQFVDVTADGFDAGIRYSGTIPEDMIAVPLTPPLKWVAVAAPDYLRSH
HCCCCEEEEEECCEEEEEECCCCCCCCEECCCCCHHCEEECCCCCCEEEEEECCHHHHHC
GRPVMPEDLNSHHCIRIRTGRGQIYKWEFERGDDRREIDVPGALISGETDLAINAALEGA
CCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCEEECCCEEECCCCCEEEEEHHCCC
GLCYCLERLASPYVSAGRLEVVLPKWASIGPPFSMYYSSRRQLPFGVDALIKIVRGLNGF
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MQFKRNEIHELSIFLAIVQYRSFRKAADHLEVTASALSHSMKALEQRLGVRLLNRTSRSV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
APTAAGTALAEKISAGLELINSGLEDLNGHYQGGAGSIRINVLKDAAVLLLRPAIPVFQQ
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHCCCCHHHHH
RFPNVELEVAVDDQFVDVTADGFDAGIRYSGTIPEDMIAVPLTPPLKWVAVAAPDYLRSH
HCCCCEEEEEECCEEEEEECCCCCCCCEECCCCCHHCEEECCCCCCEEEEEECCHHHHHC
GRPVMPEDLNSHHCIRIRTGRGQIYKWEFERGDDRREIDVPGALISGETDLAINAALEGA
CCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCEEECCCEEECCCCCEEEEEHHCCC
GLCYCLERLASPYVSAGRLEVVLPKWASIGPPFSMYYSSRRQLPFGVDALIKIVRGLNGF
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]