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The map label for this gene is fliY [H]

Identifier: 116248754

GI number: 116248754

Start: 78038

End: 78823

Strand: Direct

Name: fliY [H]

Synonym: pRL120078

Alternate gene names: 116248754

Gene position: 78038-78823 (Clockwise)

Preceding gene: 116248753

Following gene: 116248755

Centisome position: 8.97

GC content: 60.31

Gene sequence:

>786_bases
ATGCTGACAAGACGAGTCTTCTTCGCAATCGCGACCCTGGCTGCAACCTTCGGCTTCGGCTCCGCGTCTTACGCCGATGC
TCTTGCCGACATCACGGCGCGCGGTACGCTGCGTGTCGCCGTCCCGCAGGATTTTCCGCCCTTCGGTAGTGTCGGCACCG
ATATGGCGCCGATGGGCTACGATATCGATGTGGCCAATCTCATTGCCGAAAAGCTGGGCGTCAAGACCGAGCTCGTGCCG
GTCACCAGCGCCAACCGCGTTCCCTATCTGCAGACGAACAAAGTCGATCTGGTCATATCAAGCCTCGGCAAGAATGCGGA
GCGCGAAAAGGTGATCGATTTCACAACGGCCTACGCACCCTTCTTCAACGGCGTGTTCGCACCGGCCGATGTTTCGATCG
CCAAGGTGGAAGATCTCGCCGGCAAGAGCATCGGCGTCACGCGCGGCGCCGTGGAGGATCTCGAGCTGACGAAGATCGCG
CCGGCCGACGCTACGATCAAGCGTTACGAGGACAACAACGGCACTATCTCGGCCTTCCTGTCCGGCCAGGTCGACACCAT
CGCCACCGGCAACGTCGTGGCCGCTGCGATCCTCTCCAAAAATCCTCCCAAGCGCCCTGAGATGAAGTTCCTCATCAAGA
ACTCGCCCTGCTACATCGGTCTCAACAAGGAACAGACGGCCCTTCTGGAGAAGGTGAACGGCATTATCGCCGCCGCCAAA
ACCGATGGCGCGCTGAACGCCATATCGCAGAAGTGGCTCGGCGCCGATCTCCCGTCGGATCTCTAA

Upstream 100 bases:

>100_bases
CGGAACTGCGCAGTTCCCTGTCACTTCCTCCCAAGGCCGTGCCCTCGGTCTCCCAACTGTCAGACCCAAAAAACCACAAC
TTCAAAGGAGCAATTAAAAC

Downstream 100 bases:

>100_bases
GGACCGGTCTCACGGATGTCATCCCGCGCCCGCGCGGGATGACATGACGCTCCGTCGCCACAAGAGGGGACAATTCCTTG
AGTTACCATTTCGAATTCGG

Product: putative substrate-binding component of ABC transporter

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MLTRRVFFAIATLAATFGFGSASYADALADITARGTLRVAVPQDFPPFGSVGTDMAPMGYDIDVANLIAEKLGVKTELVP
VTSANRVPYLQTNKVDLVISSLGKNAEREKVIDFTTAYAPFFNGVFAPADVSIAKVEDLAGKSIGVTRGAVEDLELTKIA
PADATIKRYEDNNGTISAFLSGQVDTIATGNVVAAAILSKNPPKRPEMKFLIKNSPCYIGLNKEQTALLEKVNGIIAAAK
TDGALNAISQKWLGADLPSDL

Sequences:

>Translated_261_residues
MLTRRVFFAIATLAATFGFGSASYADALADITARGTLRVAVPQDFPPFGSVGTDMAPMGYDIDVANLIAEKLGVKTELVP
VTSANRVPYLQTNKVDLVISSLGKNAEREKVIDFTTAYAPFFNGVFAPADVSIAKVEDLAGKSIGVTRGAVEDLELTKIA
PADATIKRYEDNNGTISAFLSGQVDTIATGNVVAAAILSKNPPKRPEMKFLIKNSPCYIGLNKEQTALLEKVNGIIAAAK
TDGALNAISQKWLGADLPSDL
>Mature_261_residues
MLTRRVFFAIATLAATFGFGSASYADALADITARGTLRVAVPQDFPPFGSVGTDMAPMGYDIDVANLIAEKLGVKTELVP
VTSANRVPYLQTNKVDLVISSLGKNAEREKVIDFTTAYAPFFNGVFAPADVSIAKVEDLAGKSIGVTRGAVEDLELTKIA
PADATIKRYEDNNGTISAFLSGQVDTIATGNVVAAAILSKNPPKRPEMKFLIKNSPCYIGLNKEQTALLEKVNGIIAAAK
TDGALNAISQKWLGADLPSDL

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=263, Percent_Identity=28.1368821292776, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787085, Length=250, Percent_Identity=27.2, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1788648, Length=231, Percent_Identity=25.5411255411255, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787088, Length=230, Percent_Identity=23.4782608695652, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787031, Length=263, Percent_Identity=26.615969581749, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27598; Mature: 27598

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRRVFFAIATLAATFGFGSASYADALADITARGTLRVAVPQDFPPFGSVGTDMAPMGY
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCC
DIDVANLIAEKLGVKTELVPVTSANRVPYLQTNKVDLVISSLGKNAEREKVIDFTTAYAP
CCHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCEEEEEHHCCCCCHHHHHHHHHHHHH
FFNGVFAPADVSIAKVEDLAGKSIGVTRGAVEDLELTKIAPADATIKRYEDNNGTISAFL
HHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEE
SGQVDTIATGNVVAAAILSKNPPKRPEMKFLIKNSPCYIGLNKEQTALLEKVNGIIAAAK
CCCCCEEECCCHHHHHHHCCCCCCCCCEEEEEECCCEEEECCCHHHHHHHHHCCEEEEEC
TDGALNAISQKWLGADLPSDL
CCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLTRRVFFAIATLAATFGFGSASYADALADITARGTLRVAVPQDFPPFGSVGTDMAPMGY
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCC
DIDVANLIAEKLGVKTELVPVTSANRVPYLQTNKVDLVISSLGKNAEREKVIDFTTAYAP
CCHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCEEEEEHHCCCCCHHHHHHHHHHHHH
FFNGVFAPADVSIAKVEDLAGKSIGVTRGAVEDLELTKIAPADATIKRYEDNNGTISAFL
HHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEE
SGQVDTIATGNVVAAAILSKNPPKRPEMKFLIKNSPCYIGLNKEQTALLEKVNGIIAAAK
CCCCCEEECCCHHHHHHHCCCCCCCCCEEEEEECCCEEEECCCHHHHHHHHHCCEEEEEC
TDGALNAISQKWLGADLPSDL
CCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]